Mouse CXCL2/MIP-2 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:CXCL2, MIP-2a, GRO2, GROb
- 种属:小鼠 (Mouse)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:20 μL
- 灵敏度:5.23 pg/mL
- 检测范围:7.81 pg/mL - 500 pg/mL
- 回收率:81% - 99%
在售SKU:70-EK2142/2-48, 70-EK2142/2-96
描述
商品名 |
Mouse CXCL2/MIP-2 ELISA Kit (小鼠趋化因子CXC配体2/巨噬细胞炎症蛋白2 ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:1.2% - 6.8%;板间变异系数:2.1% - 6.0% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
20 μL |
灵敏度 |
5.23 pg/mL |
检测范围 |
7.81 pg/mL - 500 pg/mL |
回收率 |
81% - 99% |
平均回收率 |
0.92 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠MIP-2单克隆抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的MIP-2与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中MIP-2的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
CXCL2 分子靶点信息概述
- 分子名:CXCL2, C-X-C motif chemokine ligand 2
- 基因家族:Chemokine ligands
- 别名:SCYB2; GROb; MIP-2a; MGSA-b; CINC-2a
- 曾用名:GRO2
- 全称:GRO2 oncogene; chemokine (C-X-C motif) ligand 2
CXCL2 分子靶点综述
人生长调节致癌基因β (GRO-β)也称为 CXCL2,是 CXC 蛋白趋化因子家族成员之一。GRO-β 在小鼠中也称为 MIP-2,在大鼠中称为 CINC-3。人 GRO-β由 107 个氨基酸(aa)构成,其分子量约 为 11 kDa。GRO-β是由炎症部位的多种细胞(包括单核细胞和巨噬细胞)分泌,对粒细胞包括中性粒 细胞具有趋化作用。GRO-β在炎症反应和损伤修复过程中起着重要作用。研究逐渐发现 GRO 为一种原癌基因。GRO-β作为表达产物在血管新生、肿瘤形成转移和肿瘤与免疫细胞相互作用中起到关键作用。
小鼠 Mouse Cxcl2 分子靶点信息
- 分子名:Cxcl2, chemokine (C-X-C motif) ligand 2
- 别称:
- CINC-2a
- Gro2
- GROb
- macrophage inflammatory protein 2
- Mgsa-b
- MIP-2
- MIP-2a
- Mip2
- Scyb
- Scyb2
- small inducible cytokine B subfamily
- small inducible cytokine subfamily, member 2
- 基因序列:NCBI_Gene: 20310
- 蛋白序列:
小鼠 Mouse Cxcl2 靶点分子功能(预测)
Predicted to enable CXCR chemokine receptor binding activity and chemokine activity. Involved in response to molecule of bacterial origin. Located in extracellular space. Is expressed in several structures, including alimentary system; embryo mesenchyme; floor plate; respiratory system; and skin. Orthologous to several human genes including CXCL3 (C-X-C motif chemokine ligand 3).
引用文献统计
该产品被引用的文献总数为:25
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刊物:Biomed Pharmacother. 2022 Aug;152:113258.
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