EK268

Mouse CXCL10/IP-10 ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：CXCL10, INP10, IP-10
  • 种属：小鼠 (Mouse)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：血清血浆：50 μL；细胞培养上清：100 μL
  • 灵敏度：19.03 pg/mL
  • 检测范围：312.5 pg/mL - 20000 pg/mL
  • 回收率：71% - 122%

在售SKU:70-EK268/2-48, 70-EK268/2-96

描述

商品名

Mouse CXCL10/IP-10 ELISA Kit (小鼠C-X-C基序趋化因子10 ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：2.2% - 4.6%；板间变异系数：1.3% - 3.8%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

血清血浆：50 μL；细胞培养上清：100 μL
灵敏度

19.03 pg/mL
检测范围

312.5 pg/mL - 20000 pg/mL
回收率

71% - 122%
平均回收率

0.95
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗CXCL10/IP-10单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠CXCL10/IP-10冻干标准品
  • CXCL10/IP-10 检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IP-10抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IP-10与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IP-10的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse CXCL10/IP-10 ELISA Kit (小鼠C-X-C基序趋化因子10 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 CXCL10, INP10, IP-10 靶点信息

CXCL10 分子靶点信息概述

  • 分子名：CXCL10, C-X-C motif chemokine ligand 10
  • 基因家族：Chemokine ligands
  • 别名：IFI10; IP-10; crg-2; mob-1; C7; gIP-10
  • 曾用名：INP10; SCYB10
  • 全称：small inducible cytokine subfamily B (Cys-X-Cys), member 10; chemokine (C-X-C motif) ligand 10

CXCL10 分子靶点综述

趋化因子配体10(CXCL10)又称为γ-干扰素诱导蛋白10(IP-10)或微小诱导细胞因子B10，是属于CXC趋化因子家族的小分子量细胞因子。多种类型的细胞对IFN-γ产生应答后可分泌IP-10，包括单核细胞、内皮细胞和成纤维细胞等。IP-10具有多种功能，如对单核/巨噬细胞、T细胞、NK细胞和树突细胞的趋化作用，促进T细胞黏附于内皮细胞，抗肿瘤活性和抑制骨髓集落形成和血管生成。

小鼠 Mouse Cxcl10 分子靶点信息

小鼠 Mouse Cxcl10 靶点分子功能（预测）

Predicted to enable chemoattractant activity; chemokine receptor binding activity; and heparin binding activity. Acts upstream of or within several processes, including defense response to virus; negative regulation of myoblast differentiation; and negative regulation of myoblast fusion. Located in external side of plasma membrane and extracellular space. Is expressed in several structures, including alimentary system; axial skeleton; hemolymphoid system; and liver. Human ortholog(s) of this gene implicated in hepatitis B; middle cerebral artery infarction; and type 1 diabetes mellitus. Orthologous to human CXCL10 (C-X-C motif chemokine ligand 10).

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. Mast cell degranulation impairs pneumococcus clearance in mice via IL-6 dependent and TNF-α independent mechanisms 

  2. Fullerenols boosting the therapeutic effect of anti-CD47 antibody to trigger robust anti-tumor immunity by inducing calreticulin exposure 

  3. MiR-19a-3p Suppresses M1 Macrophage Polarization by Inhibiting STAT1/IRF1 Pathway 

  4. Phospho-Tyr705 of STAT3 is a therapeutic target for sepsis through regulating inflammation and coagulation 

  5. CXCL10-armed oncolytic adenovirus promotes tumor-infiltrating T-cell chemotaxis to enhance anti-PD-1 therapy 

  6. Cytosolic delivery of the immunological adjuvant Poly I:C and cytotoxic drug crystals via a carrier-free strategy significantly amplifies immune response 

  7. Lacticaseibacillus paracasei sh2020 induced antitumor immunity and synergized with anti-programmed cell death 1 to reduce tumor burden in mice 

  8. CDKN2A Deletion in Melanoma Excludes T Cell Infiltration by Repressing Chemokine Expression in a Cell Cycle-Dependent Manner 

  9. Isoforskolin Alleviates AECOPD by Improving Pulmonary Function and Attenuating Inflammation Which Involves Downregulation of Th17/IL-17A and NF-κB/NLRP3 

  10. Combination of Fruquintinib and Anti–PD-1 for the Treatment of Colorectal Cancer 

  11. miR-130b-3p regulates M1 macrophage polarization via targeting IRF1 

  12. A non-bactericidal cathelicidin provides prophylactic efficacy against bacterial infection by driving phagocyte influx 

  13. Viperin deficiency promotes dendritic cell activation and function via NF-kappaB activation during Mycobacterium tuberculosis infection 

  14. Molecular mechanisms underlying macrophage immunomodulatory activity of Rubus chingii Hu polysaccharides 

  15. Oral administration of MnCl2 attenuated hyperlipidemia-related cardiac remodeling in ApoE−/− mice 

  16. Exosome-mediated delivery of Cas9 ribonucleoprotein complexes for tissue-specific gene therapy of liver diseases 

  17. Peucedanum praeruptorum Dunn polysaccharides regulate macrophage inflammatory response through TLR2/TLR4-mediated MAPK and NF-κB pathways 

  18. CX3CL1 Recruits NK Cells Into the Central Nervous System and Aggravates Brain Injury of Mice Caused by Angiostrongylus cantonensis Infection 

  19. Inhibition of Fibroblast Growth Factor Receptor by AZD4547 Protects Against Inflammation in Septic Mice 

  20. Targeted Delivery of STING Agonist via Albumin Nanoreactor Boosts Immunotherapeutic Efficacy against Aggressive Cancers 

  21. Reconstituted CD74+ NK cells trigger chronic graft versus host disease after allogeneic bone marrow transplantation 

  22. Reversing EZH2-Mediated Epigenetic Silence with Photodynamic Polymeric Nanoparticles for Synergistically Enhanced Antitumor Immunotherapy 

  23. Psoralen attenuates cigarette smoke extract-induced inflammation by modulating CD8+ T lymphocyte recruitment and chemokines via the JAK2/STAT1 signaling pathway 

  24. Chemical conjugation mitigates immunotoxicity of chemotherapy via reducing receptor-mediated drug leakage from lipid nanoparticles 

  25. Parabacteroides distasonis-Derived Outer Membrane Vesicles Enhance Antitumor Immunity Against Colon Tumors by Modulating CXCL10 and CD8+ T Cells 

  26. HIF-1α Mediates Immunosuppression and Chemoresistance in Colorectal Cancer by Inhibiting CXCL9, ?10 and ?11 

  27. Neutrophil extracellular trap-related mechanisms in acne vulgaris inspire a novel treatment strategy with adipose-derived stem cells 

  28. MiR-9 promotes G-MDSC recruitment and tumor proliferation by targeting SOCS3 in breast cancer 

  29. Hydrogel-mediated tumor T cell infiltration and immune evasion to reinforce cancer immunotherapy 

  30. Manganese@Albumin Nanocomplex and Its Assembled Nanowire Activate TLR4-Dependent Signaling Cascades of Macrophages 

  31. Tumor Microenvironment Sequential Drug/Gene Delivery Nanosystem for Realizing Multistage Boosting of Cancer–Immunity Cycle on Cancer Immunotherapy 

  32. Immunostimulant Hydrogel-Guided Tumor Microenvironment Reprogramming to Efficiently Potentiate Macrophage-Mediated Cellular Phagocytosis for Systemic Cancer Immunotherapy 

  33. Enhancement of Microglia Functions by Developed Nano-Immuno-Synergist to Ameliorate Immunodeficiency for Malignant Glioma Treatment 

  34. FABP4 in LSECs promotes CXCL10-mediated macrophage recruitment and M1 polarization during NAFLD progression 

  35. FOSL2 promotes intertumoral infiltration of T cells and increases pathological complete response rates in locally advanced rectal cancer patients 

  36. Oroxylin A inhibited autoimmune hepatitis-induced liver injury and shifted Treg/Th17 balance to Treg differentiation 

  37. Prevention of Bleomycin-Induced Pulmonary Inflammation and Fibrosis in Mice by Bilobalide 

  38. Hypoxia-Inducible Factor 2α Attenuates Renal Ischemia-Reperfusion Injury by Suppressing CD36-Mediated Lipid Accumulation in Dendritic Cells in a Mouse Model 

  39. 细胞培养上清

    Molecular Mechanisms Underlying Macrophage Immunomodulatory Activity of Rubus Chingii Hu Polysaccharides 

  40. 细胞培养上清

    Peucedanum praeruptorum Dunn polysaccharides regulate macrophage inflammatory response through TLR2/TLR4-mediated MAPK and NF-κB pathways 

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