Mouse CCL5/RANTES ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:CCL5, RANTES, SISd
- 种属:小鼠 (Mouse)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:20 μL
- 灵敏度:0.12 pg/mL
- 检测范围:15.63 pg/mL - 1000 pg/mL
- 回收率:80% - 113%
在售SKU:70-EK2129/2-48, 70-EK2129/2-96
描述
商品名 |
Mouse CCL5/RANTES ELISA Kit (小鼠 CCL5/RANTES ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:2.6% - 3.9%;板间变异系数:3.0% - 5.8% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
20 μL |
灵敏度 |
0.12 pg/mL |
检测范围 |
15.63 pg/mL - 1000 pg/mL |
回收率 |
80% - 113% |
平均回收率 |
0.96 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠CCL5抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的CCL5与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中CCL5的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
CCL5 分子靶点信息概述
- 分子名:CCL5, C-C motif chemokine ligand 5
- 基因家族:Chemokine ligands
- 别名:RANTES; SISd; TCP228; MGC17164
- 曾用名:D17S136E; SCYA5
- 全称:T-cell specific protein p288; T-cell specific RANTES protein; SIS-delta; regulated upon activation, normally T-expressed, and presumably secreted; beta-chemokine RANTES; small inducible cytokine subfamily A (Cys-Cys), member 5; small inducible cytokine A5 (RANTES); chemokine (C-C motif) ligand 5
CCL5 分子靶点综述
趋化因子配体5(CCL5),又名RANTES,是一种趋化细胞因子。它可趋化T细胞、嗜酸性粒细胞和嗜碱性粒细胞,在招募白细胞到炎症位点发挥着积极的作用。在T细胞释放的细胞因子如IL-2和IFN-γ的帮助下,CCL5可诱导某些自然杀伤细胞(NK)增殖和活化形成CC趋化因子活化的杀伤(CHAK)细胞。CCL5也是一种由CD8+T细胞释放的HIV抑制因子。最近,研究者正在开发完善乳酸菌体内产生CCL5的方案,以期其成为抑制HIV进入的局部杀菌剂。
小鼠 Mouse Ccl5 分子靶点信息
- 分子名:Ccl5, chemokine (C-C motif) ligand 5
- 别称:
- MuRantes
- RANTES
- Scya5
- SISd
- small inducible cytokine A5
- TCP228
- 基因序列:NCBI_Gene: 20304
- 蛋白序列:
小鼠 Mouse Ccl5 靶点分子功能(预测)
Enables CCR1 chemokine receptor binding activity. Involved in negative regulation of macrophage apoptotic process and positive regulation of monocyte chemotaxis. Acts upstream of or within inflammatory response; positive regulation of epithelial cell proliferation; and response to tumor necrosis factor. Located in cytoplasm and extracellular space. Is expressed in several structures, including central nervous system; gonad; hemolymphoid system; late tubule; and retina. Human ortholog(s) of this gene implicated in several diseases, including hepatitis B; hepatitis C; liver disease (multiple); pulmonary tuberculosis; and severe acute respiratory syndrome. Orthologous to human CCL5 (C-C motif chemokine ligand 5).
操作步骤
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引用文献
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Cytosolic delivery of the immunological adjuvant Poly I:C and cytotoxic drug crystals via a carrier-free strategy significantly amplifies immune response 
miR-130b-3p regulates M1 macrophage polarization via targeting IRF1 
Hypofractionated Irradiation Suppressed the Off-Target Mouse Hepatocarcinoma Growth by Inhibiting Myeloid-Derived Suppressor Cell-Mediated Immune Suppression 
CX3CL1 Recruits NK Cells Into the Central Nervous System and Aggravates Brain Injury of Mice Caused by Angiostrongylus cantonensis Infection 
Insulin Exacerbates Inflammation in Fibroblast-Like Synoviocytes 
Monocytes Undergo Functional Reprogramming to Generate Immunosuppression through HIF-1α Signaling Pathway in the Late Phase of Sepsis 
Self-delivery nanodrug to manipulate tumor microenvironment for boosting photodynamic cancer immunotherapy 
MiR-9 promotes G-MDSC recruitment and tumor proliferation by targeting SOCS3 in breast cancer 
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Human umbilical cord mesenchymal stem cells ameliorate colon inflammation via modulation of gut microbiota-SCFAs-immune axis 
IGF2BP3 Enhances the Growth of Hepatocellular Carcinoma Tumors by Regulating the Properties of Macrophages and CD8+ T Cells in the Tumor Microenvironment 
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