EK2129

Mouse CCL5/RANTES ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:CCL5, RANTES, SISd
  • 种属:小鼠 (Mouse)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:20 μL
  • 灵敏度:0.12 pg/mL
  • 检测范围:15.63 pg/mL - 1000 pg/mL
  • 回收率:80% - 113%

在售SKU:70-EK2129/2-48, 70-EK2129/2-96


描述

商品名

Mouse CCL5/RANTES ELISA Kit (小鼠 CCL5/RANTES ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:2.6% - 3.9%;板间变异系数:3.0% - 5.8%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

20 μL

灵敏度

0.12 pg/mL

检测范围

15.63 pg/mL - 1000 pg/mL

回收率

80% - 113%

平均回收率

0.96

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗CCL5/RANTES单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠CCL5/RANTES冻干标准品
  • CCL5/RANTES检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠CCL5抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的CCL5与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中CCL5的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse CCL5/RANTES ELISA Kit (小鼠 CCL5/RANTES ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 CCL5, RANTES, SISd 靶点信息

CCL5 分子靶点信息概述

  • 分子名:CCL5, C-C motif chemokine ligand 5
  • 基因家族:Chemokine ligands
  • 别名:RANTES; SISd; TCP228; MGC17164
  • 曾用名:D17S136E; SCYA5
  • 全称:T-cell specific protein p288; T-cell specific RANTES protein; SIS-delta; regulated upon activation, normally T-expressed, and presumably secreted; beta-chemokine RANTES; small inducible cytokine subfamily A (Cys-Cys), member 5; small inducible cytokine A5 (RANTES); chemokine (C-C motif) ligand 5

CCL5 分子靶点综述

趋化因子配体5(CCL5),又名RANTES,是一种趋化细胞因子。它可趋化T细胞、嗜酸性粒细胞和嗜碱性粒细胞,在招募白细胞到炎症位点发挥着积极的作用。在T细胞释放的细胞因子如IL-2和IFN-γ的帮助下,CCL5可诱导某些自然杀伤细胞(NK)增殖和活化形成CC趋化因子活化的杀伤(CHAK)细胞。CCL5也是一种由CD8+T细胞释放的HIV抑制因子。最近,研究者正在开发完善乳酸菌体内产生CCL5的方案,以期其成为抑制HIV进入的局部杀菌剂。

小鼠 Mouse Ccl5 分子靶点信息

小鼠 Mouse Ccl5 靶点分子功能(预测)

Enables CCR1 chemokine receptor binding activity. Involved in negative regulation of macrophage apoptotic process and positive regulation of monocyte chemotaxis. Acts upstream of or within inflammatory response; positive regulation of epithelial cell proliferation; and response to tumor necrosis factor. Located in cytoplasm and extracellular space. Is expressed in several structures, including central nervous system; gonad; hemolymphoid system; late tubule; and retina. Human ortholog(s) of this gene implicated in several diseases, including hepatitis B; hepatitis C; liver disease (multiple); pulmonary tuberculosis; and severe acute respiratory syndrome. Orthologous to human CCL5 (C-C motif chemokine ligand 5).

操作步骤

ELISA操作常见问题

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引用文献

文章目录[隐藏]


  1. Endothelial cells promote triple-negative breast cancer cell metastasis via PAI-1 and CCL5 signaling 

  2. Micronized Palmitoylethanolamide Ameliorates Methionine- and Choline-Deficient Diet-Induced Nonalcoholic Steatohepatitis via Inhibiting Inflammation and Restoring Autophagy 

  3. A Network Pharmacology-Based Investigation to the Pharmacodynamic Material Basis and Mechanisms of the Anti-Inflammatory and Anti-Viral Effect of Isatis indigotica 

  4. Alanyl-Glutamine Protects Mice against Methionine- and Choline-Deficient-Diet-Induced Steatohepatitis and Fibrosis by Modulating Oxidative Stress and Inflammation 

  5. Molecular mechanisms associated with macrophage activation by Rhizoma Atractylodis Macrocephalae polysaccharides 

  6. Alanyl-Glutamine Protects against Lipopolysaccharide-Induced Liver Injury in Mice via Alleviating Oxidative Stress, Inhibiting Inflammation, and Regulating Autophagy 

  7. Plant-derived RNAi therapeutics: A strategic inhibitor of HBsAg 

  8. TMT-based quantitative proteomics reveals suppression of SLC3A2 and ATP1A3 expression contributes to the inhibitory role of acupuncture on airway inflammation in an OVA-induced mouse asthma model 

  9. Cytosolic delivery of the immunological adjuvant Poly I:C and cytotoxic drug crystals via a carrier-free strategy significantly amplifies immune response 

  10. miR-130b-3p regulates M1 macrophage polarization via targeting IRF1 

  11. Hypofractionated Irradiation Suppressed the Off-Target Mouse Hepatocarcinoma Growth by Inhibiting Myeloid-Derived Suppressor Cell-Mediated Immune Suppression 

  12. CX3CL1 Recruits NK Cells Into the Central Nervous System and Aggravates Brain Injury of Mice Caused by Angiostrongylus cantonensis Infection 

  13. Insulin Exacerbates Inflammation in Fibroblast-Like Synoviocytes 

  14. Monocytes Undergo Functional Reprogramming to Generate Immunosuppression through HIF-1α Signaling Pathway in the Late Phase of Sepsis 

  15. Self-delivery nanodrug to manipulate tumor microenvironment for boosting photodynamic cancer immunotherapy 

  16. MiR-9 promotes G-MDSC recruitment and tumor proliferation by targeting SOCS3 in breast cancer 

  17. Hepatocyte CHRNA4 mediates the MASH-promotive effects of immune cell-produced acetylcholine and smoking exposure in mice and humans 

  18. Human umbilical cord mesenchymal stem cells ameliorate colon inflammation via modulation of gut microbiota-SCFAs-immune axis 

  19. IGF2BP3 Enhances the Growth of Hepatocellular Carcinoma Tumors by Regulating the Properties of Macrophages and CD8+ T Cells in the Tumor Microenvironment 

  20. Comprehensive immune landscape of lung-resident memory CD8+ T cells after influenza infection and reinfection in a mouse model 

  21. Hypoxia-Inducible Factor 2α Attenuates Renal Ischemia-Reperfusion Injury by Suppressing CD36-Mediated Lipid Accumulation in Dendritic Cells in a Mouse Model 

  22. RAW 264.7 细胞培养上清

    A Network Pharmacology-Based Investigation to the Pharmacodynamic Material Basis and Mechanisms of the Anti-Inflammatory and Anti-Viral Effect of Isatis Indigotica 

  23. 组织匀浆 肿瘤

    A carrier-free photodynamic nanodrug to enable regulation of dendritic cells for boosting cancer immunotherapy 

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