EK287HSS

Mouse CCL2/MCP-1 High Sensitivity Standard (小鼠CCL2/MCP-1 高敏 标准品)

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¥180.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：CCL2, MCP-1, MCAF, SMC-CF
  • 种属：小鼠 (Mouse)
  • 试剂盒：EK287HS
  • 保存：短期4℃保存，长期-20℃保存
  • 运输条件：4℃蓝冰运输

在售SKU:70-EK287HSS

描述

本产品只包含标准品试剂，如需购买试剂盒请点击下图

商品名

Mouse CCL2/MCP-1 High Sensitivity Standard (小鼠CCL2/MCP-1 高敏 标准品)
组分

小鼠CCL2/MCP-1冻干标准品
检测方法

双抗夹心法
样本类型

血清，血浆，细胞培养上清及其他生物学样本
板式

管
保存

短期4℃，长期-20℃保存
运输条件

4℃蓝冰运输

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠CCL2抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的CCL2与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中CCL2的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse CCL2/MCP-1 High Sensitivity ELISA Kit (小鼠CCL2/MCP-1 高敏 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 CCL2, MCP-1, MCAF, SMC-CF 靶点信息

CCL2 分子靶点信息概述

  • 分子名：CCL2, C-C motif chemokine ligand 2
  • 基因家族：Chemokine ligands
  • 别名：MCP1; MCP-1; MCAF; SMC-CF; GDCF-2; HC11; MGC9434
  • 曾用名：SCYA2
  • 全称：monocyte chemotactic protein 1, homologous to mouse Sig-je; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; monocyte secretory protein JE; small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); chemokine (C-C motif) ligand 2

CCL2 分子靶点综述

趋化因子配体2(CCL2)，又名单核细胞趋化蛋白1(MCP-1)，是CC趋化因子家族的小分子量细胞因子。CCL2通过蛋白聚糖的粘多糖侧链锚定到内皮细胞的质膜上，主要由单核细胞、巨噬细胞和树突状细胞分泌。CCL2可招募单核细胞、记忆性T细胞和树突状细胞到组织损伤或感染引起的炎症位点。CCL2参与以单核细胞浸润为特征的一些疾病发病机制，如银屑病、类风湿性关节炎和动脉粥样硬化。CCL2参与发生在中枢神经系统的多种疾病的神经炎症过程，表现为神经元变性。

小鼠 Mouse Ccl2 分子靶点信息

小鼠 Mouse Ccl2 靶点分子功能（预测）

Enables CCR2 chemokine receptor binding activity and cytokine activity. Involved in several processes, including cellular response to cytokine stimulus; positive regulation of leukocyte chemotaxis; and positive regulation of macromolecule metabolic process. Acts upstream of or within response to bacterium and response to wounding. Located in extracellular space. Is expressed in several structures, including heart; intestine smooth muscle circular layer; nervous system; retina; and vibrissa. Used to study age related macular degeneration. Human ortholog(s) of this gene implicated in several diseases, including Henoch-Schoenlein purpura; aggressive periodontitis; autoimmune disease (multiple); gastrointestinal system cancer (multiple); and tuberculosis (multiple). Orthologous to human CCL2 (C-C motif chemokine ligand 2).

操作步骤

ELISA操作常见问题

一文掌握ELISA实验显色判断、数据分析及标曲拟合 

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. 5-Methoxyflavone alleviates LPS-mediated lung injury by promoting Nrf2-mediated the suppression of NOX4/TLR4 axis in bronchial epithelial cells and M1 polarization in macrophages 

  2. Consumption of Saturated Fatty Acids-Rich Lard Benefits Recovery of Experimental Arthritis by Activating PPAR-γ 

  3. Preferred M2 Polarization by ASC-Based Hydrogel Accelerated Angiogenesis and Myogenesis in Volumetric Muscle Loss Rats 

  4. MyD88-Dependent Signaling Is Required for HOIP Deficiency–Induced Autoinflammation 

  5. Effects of divalent cations on the physical, conformational and immunological properties of bovine allergen β-lactoglobulin aggregates 

  6. Effective Model of Food Allergy in Mice Sensitized with Ovalbumin and Freud’s Adjuvant 

  7. Eotaxin‑1 and MCP‑1 serve as circulating indicators in response to power frequency electromagnetic field exposure in mice 

  8. Card9 protects fungal peritonitis through regulating Malt1-mediated activation of autophagy in macrophage 

  9. Mep1a contributes to Ang II-induced cardiac remodeling by promoting cardiac hypertrophy, fibrosis and inflammation 

  10. Dietary Supplementation of Foxtail Millet Ameliorates Colitis-Associated Colorectal Cancer in Mice via Activation of Gut Receptors and Suppression of the STAT3 Pathway 

  11. pH-Switchable Antimicrobial Supramolecular Hydrogels for Synergistically Eliminating Biofilm and Promoting Wound Healing 

  12. Micronized Palmitoylethanolamide Ameliorates Methionine- and Choline-Deficient Diet-Induced Nonalcoholic Steatohepatitis via Inhibiting Inflammation and Restoring Autophagy 

  13. Alanyl-Glutamine Protects Mice against Methionine- and Choline-Deficient-Diet-Induced Steatohepatitis and Fibrosis by Modulating Oxidative Stress and Inflammation 

  14. TNF-Like Ligand 1 Aberrance Aggravates Nonalcoholic Steatohepatitis via M1 Macrophage Polarization 

  15. Colitis aggravated by Mrgprb2 knockout is associated with altered immune response, intestinal barrier function and gut microbiota 

  16. Watsonianone A from Rhodomyrtus tomentosa Fruit Attenuates Respiratory-Syncytial-Virus-Induced Inflammation In Vitro 

  17. Loganin prevents BV-2 microglia cells from Aβ1-42-induced inflammation via regulating TLR4/TRAF6/NF-κB axis 

  18. Indium oxide nanoparticles induce lung intercellular toxicity between bronchial epithelial cells and macrophages 

  19. Overexpressing six-transmembrane protein of prostate 2 (STAMP2) alleviates sepsis-induced acute lung injury probably by hindering M1 macrophage polarization via the NF-κB pathway 

  20. Molecular mechanisms associated with macrophage activation by Rhizoma Atractylodis Macrocephalae polysaccharides 

  21. TAB1 regulates glycolysis and activation of macrophages in diabetic nephropathy 

  22. Administration of isoliquiritigenin prevents nonalcoholic fatty liver disease through a novel IQGAP2-CREB-SIRT1 axis 

  23. High-mobility group box 1 protein participates in acute lung injury by activating protein kinase R and inducing M1 polarization 

  24. GTS-21 Reduces Inflammation in Acute Lung Injury by Regulating M1 Polarization and Function of Alveolar Macrophages 

  25. Branched-chain amino acid transaminase 1 inhibition attenuates childhood asthma in mice by effecting airway remodeling and autophagy 

  26. Modulation of the Crosstalk between Schwann Cells and Macrophages for Nerve Regeneration: A Therapeutic Strategy Based on a Multifunctional Tetrahedral Framework Nucleic Acids System 

  27. Chemical component and in vitro protective effects of Matricaria chamomilla (L.) against lipopolysaccharide insult 

  28. OLFM4 Regulates Lung Epithelial Cell Function in Sepsis-Associated ARDS/ALI via LDHA-Mediated NF-κB Signaling 

  29. MiR-126a-5p limits the formation of abdominal aortic aneurysm in mice and decreases ADAMTS-4 expression 

  30. A Frog-Derived Cathelicidin Peptide with Dual Antimicrobial and Immunomodulatory Activities Effectively Ameliorates Staphylococcus aureus-Induced Peritonitis in Mice 

  31. Remodeling on adipocytic physiology of organophosphorus esters in mature adipocytes 

  32. Anti-inflammatory activity of the Tongmai Yangxin pill in the treatment of coronary heart disease is associated with estrogen receptor and NF-κB signaling pathway 

  33. Exosome-mediated delivery of Cas9 ribonucleoprotein complexes for tissue-specific gene therapy of liver diseases 

  34. Fluorous-Tagged Peptide Nanoparticles Ameliorate Acute Lung Injury via Lysosomal Stabilization and Inflammation Inhibition in Pulmonary Macrophages 

  35. A reduction of Syndecan-4 in macrophages promotes atherosclerosis by aggravating the proinflammatory capacity of macrophages 

  36. HMGB1 aggravates lipopolysaccharide-induced acute lung injury through suppressing the activity and function of Tregs 

  37. GYY4137 ameliorates sepsis-induced cardiomyopathy via NLRP3 pathway 

  38. Platelet-derived growth factor B attenuates lethal sepsis through inhibition of inflammatory responses 

  39. Bovine α-lactalbumin hydrolysates (α-LAH) attenuate high-fat diet induced nonalcoholic fatty liver disease by modulating hepatic lipid metabolism in C57BL/6J mice 

  40. CX3CL1 Recruits NK Cells Into the Central Nervous System and Aggravates Brain Injury of Mice Caused by Angiostrongylus cantonensis Infection 

  41. Glycyrrhetinic acid regulates impaired macrophage autophagic flux in the treatment of non-alcoholic fatty liver disease 

  42. MiR-340 suppresses CCl4-induced acute liver injury through exerting anti-inflammation targeting Sigirr 

  43. Loss of Annexin A1 in macrophages restrains efferocytosis and remodels immune microenvironment in pancreatic cancer by activating the cGAS/STING pathway 

  44. M2 macrophage infusion ameliorates diabetic glomerulopathy via the JAK2/STAT3 pathway in db/db mice 

  45. PCLAF induces bone marrow adipocyte senescence and contributes to skeletal aging 

  46. Periodontal pathogen Fusobacterium nucleatum infection accelerates hepatic steatosis in high-fat diet-fed ApoE knockout mice by inhibiting Nrf2/Keap1 signaling 

  47. Systematic sequence characterization of enzymatic-derived soybean peptides for precision enhancement of anti-inflammatory properties 

  48. Epithelial cells derived exosomal miR-203a-3p facilitates stromal inflammation of type IIIA chronic prostatitis/chronic pelvic pain syndrome by targeting DUSP5 and increasing MCP-1 generation 

  49. NLRP3 inflammasome-mediated premature immunosenescence drives diabetic vascular aging dependent on the induction of perivascular adipose tissue dysfunction 

  50. A dual-targeting therapeutic nanobubble for imaging-guided atherosclerosis treatment 

  51. An amphibian-derived cathelicidin accelerates cutaneous wound healing through its main regulatory effect on phagocytes 

  52. New insight into prurigo nodularis: Proadrenomedullin N-terminal 20 peptide mediates mouse mast cell activation via Mrgprb2 

  53. Myeloid-derived grancalcin instigates obesity-induced insulin resistance and metabolic inflammation in male mice 

  54. TIFA contributes to periodontitis in diabetic mice via activating the NF?κB signaling pathway 

  55. Regulator of G protein signaling protein 6 alleviates acute lung injury by inhibiting inflammation and promoting cell self-renewal in mice 

  56. Manganese@Albumin Nanocomplex and Its Assembled Nanowire Activate TLR4-Dependent Signaling Cascades of Macrophages 

  57. MMP10 alleviates non-alcoholic steatohepatitis by regulating macrophage M2 polarization 

  58. Alanyl-Glutamine Dipeptide Attenuates Non-Alcoholic Fatty Liver Disease Induced by a High-Fat Diet in Mice by Improving Gut Microbiota Dysbiosis 

  59. Allicin ameliorates sepsis-induced acute kidney injury through Nrf2/HO-1 signaling pathway 

  60. Ferroptosis of macrophages facilitates bone loss in apical periodontitis via NRF2/FSP1/ROS pathway 

  61. Protective effect of phosphorylated Athyrium multidentatum (Doll.) Ching polysaccharide on vascular endothelial cells in vitro and in vivo 

  62. The activation of CaN/NFAT signaling pathway in macrophages aggravated Lactobacillus casei cell wall extract-induced Kawasaki disease vasculitis 

  63. Effects of total alkaloids from Alstonia scholaris (L.) R. Br. on ovalbumin-induced asthma mice 

  64. ATP-citrate lyase controls endothelial gluco-lipogenic metabolism and vascular inflammation in sepsis-associated organ injury 

  65. Trichosanates A–G and cucurbitacins W–Y, anticomplement monoterpenoids and cucurbitane-type triterpenoids from the pericarps of Trichosanthes kirilowii 

  66. LMCD1 is involved in tubulointerstitial inflammation in the early phase of renal fibrosis by promoting NFATc1-mediated NLRP3 activation 

  67. Macrophage scavenger receptor A1 antagonizes abdominal aortic aneurysm via upregulating IRG1 

  68. Exogenous Nucleotides Ameliorate Ageing-Related Intestinal Inflammation in Senescence-Accelerated Mouse Prone-8 (SAMP8) Mice 

  69. The efficacy and mechanism of salmeterol against influenza A virus in vitro and in vivo 

  70. Discovery of the First-in-Class Intestinal Restricted FXR and FABP1 Dual Modulator ZLY28 for the Treatment of Nonalcoholic Fatty Liver Disease 

  71. P2rx1 deficiency alleviates acetaminophen-induced acute liver failure by regulating the STING signaling pathway 

  72. Inhibition of interleukin-6 trans-signaling improves survival and prevents cognitive impairment in a mouse model of sepsis 

  73. Oroxylin A inhibited autoimmune hepatitis-induced liver injury and shifted Treg/Th17 balance to Treg differentiation 

  74. Prevention of Bleomycin-Induced Pulmonary Inflammation and Fibrosis in Mice by Bilobalide 

  75. Myricetin alleviated immunologic contact urticaria and mast cell degranulation via the PI3K/Akt/NF-κB pathway 

  76. TREM2hi resident macrophages protect the septic heart by maintaining cardiomyocyte homeostasis 

  77. Hypoxia-Inducible Factor 2α Attenuates Renal Ischemia-Reperfusion Injury by Suppressing CD36-Mediated Lipid Accumulation in Dendritic Cells in a Mouse Model 

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