Mouse Adiponectin ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:ADIPOQ, ACRP30, GBP28
- 种属:小鼠 (Mouse)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:10 μL
- 灵敏度:0.73 pg/mL
- 检测范围:31.25 pg/mL - 2000 pg/mL
- 回收率:92% - 113%
在售SKU:70-EK295/2-48, 70-EK295/2-96
描述
商品名 |
Mouse Adiponectin/Acrp30 ELISA Kit (小鼠Adiponectin/Acrp30 ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:5.1% - 8.8%;板间变异系数:7.3% - 7.7% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
10 μL |
灵敏度 |
0.73 pg/mL |
检测范围 |
31.25 pg/mL - 2000 pg/mL |
回收率 |
92% - 113% |
平均回收率 |
1.07 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠 Adiponectin 抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的 Adiponectin 与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物 TMB,避光显色。颜色反应的深浅与样本中 Adiponectin 的浓度成正比。加入终止液终止反应,在 450 nm 波长(参考波长 570 - 630nm)测定吸光度值。
分子信息
ADIPOQ 分子靶点信息概述
- 分子名:ADIPOQ, adiponectin, C1Q and collagen domain containing
- 基因家族:C1q and TNF related
- 别名:ACRP30; apM1; GBP28; adiponectin
- 曾用名:ACDC
- 全称:adipose most abundant gene transcript 1; adiponectin precursor; adipocyte, C1Q and collagen domain containing
ADIPOQ 分子靶点综述
脂联素,又称脂肪细胞补体相关蛋白30(Acrp30),参与调节葡萄糖水平和脂肪酸分解。它组装成不同的复合物包括三聚体、六聚体和高阶寡聚体结构,进而可能影响生物活性。脂联素是一种蛋白质激素,调节许多代谢过程,包括葡萄糖调节和脂肪酸氧化。脂肪组织(以及妊娠期的胎盘)专门分泌脂联素到血液中,因此相对于其它许多激素,血浆中的脂联素含量很丰富。脂联素可能具有抗动脉粥样硬化和消炎的作用,可负向调节粒单祖细胞的生长和TNF-α在巨噬细胞中的产生。监测一些病理状态下的血清脂联素水平,如2型糖尿病、肥胖、动脉粥样硬化和非酒精性脂肪肝疾病,可为我们了解脂联素提供更详细的见解。
小鼠 Mouse Adipoq 分子靶点信息
- 分子名:Adipoq, adiponectin, C1Q and collagen domain containing
- 别称:
- Acdc
- Acrp30
- Ad
- adipo
- adipocyte complement related protein
- adipocyte, C1Q and collagen domain containing
- adiponectin
- apM1
- APN
- GBP28
- 基因序列:NCBI_Gene: 11450
- 蛋白序列:
小鼠 Mouse Adipoq 靶点分子功能(预测)
Enables hormone activity and identical protein binding activity. Involved in several processes, including detection of oxidative stress; negative regulation of cold-induced thermogenesis; and regulation of protein serine/threonine kinase activity. Acts upstream of or within with a positive effect on gene expression. Acts upstream of or within several processes, including brown fat cell differentiation; negative regulation of gluconeogenesis; and positive regulation of glucose import. Located in endoplasmic reticulum and extracellular space. Is expressed in several structures, including adipose tissue; axial musculature; gut; male reproductive gland or organ; and serum. Human ortholog(s) of this gene implicated in several diseases, including carcinoma (multiple); cardiovascular system disease (multiple); non-alcoholic fatty liver disease; primary open angle glaucoma; and type 2 diabetes mellitus. Orthologous to human ADIPOQ (adiponectin, C1Q and collagen domain containing).
操作步骤
文章目录[隐藏]
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引用文献
文章目录[隐藏]
- Weight-Reducing Effect of Lactobacillus Plantarum ZJUFT17 Isolated from Sourdough Ecosystem 
- Anti-diabetic activities of catalpol in db/db mice 
- Pyrocincholic acid 3β-O-β-d-quinovopyranosyl-28-O-β-d-glucopyranoside suppresses adipogenesis and regulates lipid metabolism in 3T3-L1 adipocytes 
- Chronic high-dosage fish oil exacerbates gut–liver axis injury in alcoholic steatohepatitis in mice: the roles of endotoxin and IL-4 in Kupffer cell polarization imbalance 
- A high-fat, high-protein diet attenuates the negative impact of casein-induced chronic inflammation on testicular steroidogenesis and sperm parameters in adult mice 
- Remodeling on adipocytic physiology of organophosphorus esters in mature adipocytes 
- SIRT1 inhibitors within Qing-Luo-Yin alleviated white adipose tissues-mediated inflammation in antigen-induced arthritis mice 
- Injectable cell-targeting fiber rods to promote lipolysis and regulate inflammation for obesity treatment 
- CircHOMER1 inhibits porcine adipogenesis via the miR-23b/SIRT1 axis 
Weight-Reducing Effect of Lactobacillus Plantarum ZJUFT17 Isolated from Sourdough Ecosystem 
Anti-diabetic activities of catalpol in db/db mice 
Pyrocincholic acid 3β-O-β-d-quinovopyranosyl-28-O-β-d-glucopyranoside suppresses adipogenesis and regulates lipid metabolism in 3T3-L1 adipocytes 
Chronic high-dosage fish oil exacerbates gut–liver axis injury in alcoholic steatohepatitis in mice: the roles of endotoxin and IL-4 in Kupffer cell polarization imbalance 
A high-fat, high-protein diet attenuates the negative impact of casein-induced chronic inflammation on testicular steroidogenesis and sperm parameters in adult mice 
Remodeling on adipocytic physiology of organophosphorus esters in mature adipocytes 
SIRT1 inhibitors within Qing-Luo-Yin alleviated white adipose tissues-mediated inflammation in antigen-induced arthritis mice 
Injectable cell-targeting fiber rods to promote lipolysis and regulate inflammation for obesity treatment 
CircHOMER1 inhibits porcine adipogenesis via the miR-23b/SIRT1 axis 
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