EK295

Mouse Adiponectin ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:ADIPOQ, ACRP30, GBP28
  • 种属:小鼠 (Mouse)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:10 μL
  • 灵敏度:0.73 pg/mL
  • 检测范围:31.25 pg/mL - 2000 pg/mL
  • 回收率:92% - 113%

在售SKU:70-EK295/2-48, 70-EK295/2-96


描述

商品名

Mouse Adiponectin/Acrp30 ELISA Kit (小鼠Adiponectin/Acrp30 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:5.1% - 8.8%;板间变异系数:7.3% - 7.7%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

10 μL

灵敏度

0.73 pg/mL

检测范围

31.25 pg/mL - 2000 pg/mL

回收率

92% - 113%

平均回收率

1.07

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗Adiponectin/Acrp30单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠Adiponectin/Acrp30冻干标准品
  • Adiponectin/Acrp30检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠 Adiponectin 抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的 Adiponectin 与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物 TMB,避光显色。颜色反应的深浅与样本中 Adiponectin 的浓度成正比。加入终止液终止反应,在 450 nm 波长(参考波长 570 - 630nm)测定吸光度值。

Mouse Adiponectin/Acrp30 ELISA Kit (小鼠Adiponectin/Acrp30 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 ADIPOQ, ACRP30, GBP28 靶点信息

ADIPOQ 分子靶点信息概述

  • 分子名:ADIPOQ, adiponectin, C1Q and collagen domain containing
  • 基因家族:C1q and TNF related
  • 别名:ACRP30; apM1; GBP28; adiponectin
  • 曾用名:ACDC
  • 全称:adipose most abundant gene transcript 1; adiponectin precursor; adipocyte, C1Q and collagen domain containing

ADIPOQ 分子靶点综述

脂联素,又称脂肪细胞补体相关蛋白30(Acrp30),参与调节葡萄糖水平和脂肪酸分解。它组装成不同的复合物包括三聚体、六聚体和高阶寡聚体结构,进而可能影响生物活性。脂联素是一种蛋白质激素,调节许多代谢过程,包括葡萄糖调节和脂肪酸氧化。脂肪组织(以及妊娠期的胎盘)专门分泌脂联素到血液中,因此相对于其它许多激素,血浆中的脂联素含量很丰富。脂联素可能具有抗动脉粥样硬化和消炎的作用,可负向调节粒单祖细胞的生长和TNF-α在巨噬细胞中的产生。监测一些病理状态下的血清脂联素水平,如2型糖尿病、肥胖、动脉粥样硬化和非酒精性脂肪肝疾病,可为我们了解脂联素提供更详细的见解。

小鼠 Mouse Adipoq 分子靶点信息

小鼠 Mouse Adipoq 靶点分子功能(预测)

Enables hormone activity and identical protein binding activity. Involved in several processes, including detection of oxidative stress; negative regulation of cold-induced thermogenesis; and regulation of protein serine/threonine kinase activity. Acts upstream of or within with a positive effect on gene expression. Acts upstream of or within several processes, including brown fat cell differentiation; negative regulation of gluconeogenesis; and positive regulation of glucose import. Located in endoplasmic reticulum and extracellular space. Is expressed in several structures, including adipose tissue; axial musculature; gut; male reproductive gland or organ; and serum. Human ortholog(s) of this gene implicated in several diseases, including carcinoma (multiple); cardiovascular system disease (multiple); non-alcoholic fatty liver disease; primary open angle glaucoma; and type 2 diabetes mellitus. Orthologous to human ADIPOQ (adiponectin, C1Q and collagen domain containing).

操作步骤

ELISA操作常见问题

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引用文献


  1. Weight-Reducing Effect of Lactobacillus Plantarum ZJUFT17 Isolated from Sourdough Ecosystem 

  2. Anti-diabetic activities of catalpol in db/db mice 

  3. Pyrocincholic acid 3β-O-β-d-quinovopyranosyl-28-O-β-d-glucopyranoside suppresses adipogenesis and regulates lipid metabolism in 3T3-L1 adipocytes 

  4. Chronic high-dosage fish oil exacerbates gut–liver axis injury in alcoholic steatohepatitis in mice: the roles of endotoxin and IL-4 in Kupffer cell polarization imbalance 

  5. A high-fat, high-protein diet attenuates the negative impact of casein-induced chronic inflammation on testicular steroidogenesis and sperm parameters in adult mice 

  6. Remodeling on adipocytic physiology of organophosphorus esters in mature adipocytes 

  7. SIRT1 inhibitors within Qing-Luo-Yin alleviated white adipose tissues-mediated inflammation in antigen-induced arthritis mice 

  8. Injectable cell-targeting fiber rods to promote lipolysis and regulate inflammation for obesity treatment 

  9. CircHOMER1 inhibits porcine adipogenesis via the miR-23b/SIRT1 axis 

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