EK183

Human VEGF ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥10,800.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：VEGFA, VEGF, VPF
  • 种属：人 (Human)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：50 μL
  • 灵敏度：0.95 pg/mL
  • 检测范围：31.25 pg/mL - 2000 pg/mL
  • 回收率：77% - 119%

在售SKU:70-EK183-48, 70-EK183-96

描述

商品名

Human VEGF ELISA Kit (人血管内皮生长因子 (VEGF) ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：3.3% - 4.5%；板间变异系数：4.2% - 6.8%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

50 μL
灵敏度

0.95 pg/mL
检测范围

31.25 pg/mL - 2000 pg/mL
回收率

77% - 119%
平均回收率

1
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗VEGF单克隆抗体的96孔聚苯乙烯酶标板
  • 人VEGF冻干标准品
  • VEGF检测抗体
  • 标准品稀释液
  • 辣根过氧化物酶标记的链霉亲和素
  • 信号增强剂浓缩液
  • 信号增强剂稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人VEGF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的VEGF与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中VEGF的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human VEGF ELISA Kit (人血管内皮生长因子 (VEGF) ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 VEGFA, VEGF, VPF 靶点信息

VEGFA 分子靶点信息概述

  • 分子名：VEGFA, vascular endothelial growth factor A
  • 基因家族：VEGF family
  • 别名：VEGF-A; VPF
  • 曾用名：VEGF
  • 全称：vascular endothelial growth factor

VEGFA 分子靶点综述

血管内皮生长因子(VEGF)是生长因子的亚家族，具体来说，属于胱氨酸结生长因子中的血小板衍生生长因子(PDGF)家族。它们是重要的信号转导蛋白，参与血管发生(胚胎循环系统从头形成)和血管生成(从已存在的脉管系统生长形成血管)。VEGF可刺激血管发生和血管生成。当血液循环不足时，可参与回复组织供氧系统。VEGF最主要的作用是血管生成。在胚胎形成期，VEGF调控内皮细胞的增殖、迁移和存活，从而调控血管密度和大小，但无法决定血管的类型。在支气管哮喘和糖尿病患者的血清中含有高浓度VEGF。当VEGF过表达时会引起疾病。供血不足时，实体肿瘤的大小受限；而表达VEGF的肿瘤则会不断生长并发生转移。VEGF过表达会导致眼睛视网膜和身体其他部分的血管性疾病。贝伐珠单抗和雷珠单抗可抑制VEGF，并控制或减缓疾病进程。

人 Human VEGFA 分子靶点信息

  • 分子名：VEGFA, vascular endothelial growth factor A
  • 别称：
    • MGC70609
    • MVCD1
    • vascular endothelial growth factor
    • vascular endothelial growth factor A121
    • vascular endothelial growth factor A165
    • vascular permeability factor
    • VEGF
    • VEGF-A
    • VPF
  • 基因序列：NCBI_Gene: 7422
  • 蛋白序列：UniProtKB: P15692

人 Human VEGFA靶点分子功能（预测）

Enables several functions, including fibronectin binding activity; heparin binding activity; and signaling receptor binding activity. Involved in several processes, including positive regulation of cell migration; positive regulation of intracellular signal transduction; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including branching involved in blood vessel morphogenesis; positive regulation of endothelial cell proliferation; and regulation of nitric oxide mediated signal transduction. Located in several cellular components, including adherens junction; cell surface; and secretory granule. Implicated in several diseases, including autoimmune disease (multiple); gastrointestinal system cancer (multiple); hematologic cancer (multiple); hypertension (multiple); and reproductive organ cancer (multiple). Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); inflammatory bowel disease (multiple); and respiratory system cancer (multiple).

操作步骤


下载本产品说明书

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. BRAFV600E mutant colorectal cancer cells mediate local immunosuppressive microenvironment through exosomal long noncoding RNAs 

  2. The coupling of reduced type H vessels with unloading-induced bone loss and the protection role of Panax quinquefolium saponin in the male mice 

  3. Human Umbilical Cord-Derived Mesenchymal Stem Cells Ameliorate Skin Aging of Nude Mice Through Autophagy-Mediated Anti-Senescent Mechanism 

  4. Polydopamine-modified decellularized intestinal scaffolds loaded with adipose-derived stem cells promote intestinal regeneration 

  5. Macrophage Polarization Mediated by Chitooligosaccharide (COS) and Associated Osteogenic and Angiogenic Activities 

  6. ACSL4 promotes colorectal cancer and is a potential therapeutic target of emodin 

  7. Comparison of the therapeutic effects of human umbilical cord blood-derived mesenchymal stem cells and adipose-derived stem cells on erectile dysfunction in a rat model of bilateral cavernous nerve injury. 

  8. In Vitro Angiogenic Behavior of HUVECs on Biomimetic SF/SA Composite Scaffolds 

  9. Tanshinone IIA inhibits β-catenin/VEGF-mediated angiogenesis by targeting TGF-β1 in normoxic and HIF-1α in hypoxic microenvironments in human colorectal cancer 

  10. Transverse Tibial Bone Transport Enhances Distraction Osteogenesis and Vascularization in the Treatment of Diabetic Foot 

  11. Utilizing 3D bioprinted platelet-rich fibrin-based materials to promote the regeneration of oral soft tissue 

  12. Involvement of activation of C-met signaling pathway in CD151-induced HUVECs angiogenesis 

  13. Advanced-platelet-rich fibrin extract promotes adipogenic and osteogenic differentiation of human adipose-derived stem cells in a dose-dependent manner in vitro 

  14. 590 nm LED Irradiation Improved Erythema through Inhibiting Angiogenesis of Human Microvascular Endothelial Cells and Ameliorated Pigmentation in Melasma 

  15. Biological responses of human bone mesenchymal stem cells to Ti and TiZr implant materials 

  16. Dose-dependent effects of tetramethylpyrazine on the characteristics of human umbilical cord mesenchymal stem cells for stroke therapy 

  17. Piperlongumine induces apoptosis and reduces bortezomib resistance by inhibiting STAT3 in multiple myeloma cells 

  18. Frozen bean curd-inspired xenogeneic acellular dermal matrix with triple pretreatment approach of freeze–thaw, laser drilling and ADSCs pre-culture for promoting early vascularization and integration 

  19. MSC-derived sEVs enhance patency and inhibit calcification of synthetic vascular grafts by immunomodulation in a rat model of hyperlipidemia 

  20. miR-524-5p inhibits angiogenesis through targeting WNK1 in colon cancer cells 

  21. Immunomodulatory effects of orally administrated florfenicol in rainbow trout (Oncorhynchus mykiss) following experimental challenge with streptococcosis/lactococcosis 

  22. New microtubulin inhibitor MT189 suppresses angiogenesis via the JNK-VEGF/VEGFR2 signaling axis 

  23. Tie1 contributes to the development of ovarian hyperstimulation syndrome under the regulation of EGR1 in granulosa cells 

  24. Tanshinone I inhibits tumor angiogenesis by reducing STAT3 phosphorylation at TYR705 and hypoxia-induced HIF-1α accumulation in both endothelial and tumor cells 

  25. The N6-Methyladenosine Regulator ALKBH5 Mediated Stromal Cell–Macrophage Interaction via VEGF Signaling to Promote Recurrent Spontaneous Abortion: A Bioinformatic and In Vitro Study 

  26. SRSF3 Promotes Angiogenesis in Colorectal Cancer by Splicing SRF 

  27. ALKBH5 Promotes Multiple Myeloma Tumorigenicity through inducing m6A-demethylation of SAV1 mRNA and Myeloma Stem Cell Phenotype 

  28. Inhibition of SIRT2 limits tumour angiogenesis via inactivation of the STAT3/VEGFA signalling pathway 

  29. Low-level laser therapy induces human umbilical vascular endothelial cell proliferation, migration and tube formation through activating the PI3K/Akt signaling pathway 

  30. Sanhuang Decoction Controls Tumor Microenvironment by Ameliorating Chronic Stress in Breast Cancer: A Report of Ninety Cases 

  31. Drug depot-anchoring hydrogel: A self-assembling scaffold for localized drug release and enhanced stem cell differentiation 

  32. Impacts of chitosan oligosaccharide (COS) on angiogenic activities 

  33. CircKDM4B suppresses breast cancer progression via the miR-675/NEDD4L axis 

  34. Photobiomodulation promotes angiogenesis in wound healing through stimulating the nuclear translocation of VEGFR2 and STAT3 

  35. Asparagus Polysaccharide Suppresses the Migration, Invasion, and Angiogenesis of Hepatocellular Carcinoma Cells Partly by Targeting the HIF-1α/VEGF Signalling Pathway In Vitro 

  36. Regulatory effect of microRNA-34a on osteogenesis and angiogenesis in glucocorticoid-induced osteonecrosis of the femoral head 

  37. Downregulation of glycoprotein non-metastatic melanoma protein B prevents high glucose-induced angiogenesis in diabetic retinopathy 

  38. NADH-Cytochrome B5 reductase 2 suppresses retinal vascular dysfunction through regulation of vascular endothelial growth factor A in diabetic retinopathy 

  39. Activating CD137 Signaling Promotes Sprouting Angiogenesis via Increased VEGFA Secretion and the VEGFR2/Akt/eNOS Pathway 

  40. B7-H3 expression in breast cancer and upregulation of VEGF through gene silence 

  41. CRHBP is degraded via autophagy and exerts anti-hepatocellular carcinoma effects by reducing cyclin B2 expression and dissociating cyclin B2-CDK1 complex 

  42. ATAD2 silencing decreases VEGFA secretion through targeting has-miR-520a to inhibit angiogenesis in colorectal cancer 

  43. Tetramethylpyrazine and Paeoniflorin Inhibit Oxidized LDL-Induced Angiogenesis in Human Umbilical Vein Endothelial Cells via VEGF and Notch Pathways 

  44. The loading of C-type natriuretic peptides improved hemocompatibility and vascular regeneration of electrospun poly(ε-caprolactone) grafts 

  45. Anti-angiogenic nano-delivery system promotes tumor vascular normalizing and micro-environment reprogramming in solid tumor 

  46. Correlations of serum VEGF and MMP-2 levels with CLM in CRC patients and effects of TACE on their expressions. 

  47. Influence of Cytochrome P450 2D6 Polymorphisms on the Efficacy of Oral Propranolol in Treating Infantile Hemangioma 

  48. VEGF-Modified PVA/Silicone Nanofibers Enhance Islet Function Transplanted in Subcutaneous Site Followed by Device-Less Procedure 

  49. NINJ1 triggers extravillous trophoblast cell dysfunction through blocking the STAT3 signaling pathway 

  50. The efficacy and safety of intravitreal injection of Ranibizumab as pre-treatment for vitrectomy in proliferative diabetic retinopathy with vitreous hemorrhage 

  51. Cancer-Associated Fibroblasts Promote Angiogenesis of Hepatocellular Carcinoma by VEGF-Mediated EZH2/VASH1 Pathway 

  52. Asparagus Polysaccharide inhibits the Hypoxia-induced migration, invasion and angiogenesis of Hepatocellular Carcinoma Cells partly through regulating HIF1α/VEGF expression via MAPK and PI3K signaling pathway 

  53. Lipopolysaccharide alters VEGF-A secretion of mesenchymal stem cells via the integrin β3-PI3K-AKT pathway 

  54. To improve the angiogenesis of endothelial cells on Ti-Cu alloy by the synergistic effects of Cu ions release and surface nanostructure 

  55. Determination of Vascular Endothelial Growth Factor (VEGF) in Cell Culture Medium by Gold-Coated Magnetic Nanoparticle Based Label-Free Electrochemical Impedance Spectroscopy (EIS) 

  56. A novel long-term intravenous combined with local treatment with human amnion-derived mesenchymal stem cells for a multidisciplinary rescued uremic calciphylaxis patient and the underlying mechanism 

  57. LncRNA-HNF1A-AS1 functions as a competing endogenous RNA to activate PI3K/AKT signalling pathway by sponging miR-30b-3p in gastric cancer 

  58. Sprouty2 regulates proliferation and survival of multiple myeloma by inhibiting activation of the ERK1/2 pathway in vitro and in vivo 

  59. α-Mangostin Alleviated HIF-1α-Mediated Angiogenesis in Rats With Adjuvant-Induced Arthritis by Suppressing Aerobic Glycolysis. 

  60. Elevated latent transforming growth factor beta binding protein 2 in endometriosis promotes endometrial stromal cell invasion and proliferation via the NF-kB signaling pathway 

  61. Alantolactone induces G1 phase arrest and apoptosis of multiple myeloma cells and overcomes bortezomib resistance 

  62. Metformin enhances gefitinib efficacy by interfering with interactions between tumor-associated macrophages and head and neck squamous cell carcinoma cells 

  63. Helicobacter pylori induces gastric cancer via down-regulating miR-375 to inhibit dendritic cell maturation 

  64. Effect of laser therapy on expression of angio- and fibrogenic factors, and cytokine concentrations during the healing process of human pressure ulcers 

  65. Hypoxia modifies the polarization of macrophages and their inflammatory microenvironment, and inhibits malignant behavior in cancer cells 

  66. Extracellular vesicles secreted by hypoxia pre-challenged mesenchymal stem cells promote non-small cell lung cancer cell growth and mobility as well as macrophage M2 polarization via miR-21-5p delivery 

  67. Effects of triptolide on bone marrow‑derived mesenchymal stem cells from patients with multiple myeloma 

  68. Heterogeneous porous hypoxia-mimicking scaffolds propel urethral reconstruction by promoting angiogenesis and regulating inflammation 

  69. Using Dual-Target rTMS, Single-Target rTMS, or Sham rTMS on Post-Stroke Cognitive Impairment 

  70. Knockdown of miR-1293 attenuates lung adenocarcinoma angiogenesis via Spry4 upregulation–mediated ERK1/2 signaling inhibition 

  71. ROS-scavenging lipid-based liquid crystalline as a favorable stem cell extracellular vesicles delivery vector to promote wound healing 

  72. Providing biomimetic microenvironment for pulp regeneration via hydrogel-mediated sustained delivery of tissue-specific developmental signals 

  73. Cysteine protease inhibitor S promotes lymph node metastasis of esophageal cancer cells via VEGF-MAPK/ERK-MMP9/2 pathway 

  74. Regulation of the immune microenvironment by pioglitazone-loaded polylactic glycolic acid nanosphere composite scaffolds to promote vascularization and bone regeneration 

  75. Simulated gastrointestinal digestion of walnut protein yields anti-inflammatory peptides 

  76. Brucea javanica derived exosome-like nanovesicles deliver miRNAs for cancer therapy 

  77. Helicobacter pylori-induced fibroblast-derived Serpin E1 promotes gastric cancer growth and peritoneal dissemination through p38 MAPK/VEGFA-mediated angiogenesis 

  78. EXOSC2 Mediates the Pro-tumor Role of WTAP in Breast Cancer Cells via Activating the Wnt/β-Catenin Signal 

  79. Immunomodulatory effect of Ti-Cu alloy by surface nanostructure synergistic with Cu2+ release 

  80. Rab27b promotes endometriosis by enhancing invasiveness of ESCs and promoting angiogenesis 

  81. Dihydroartemisinin inhibits angiogenesis in breast cancer via regulating VEGF and MMP-2/-9 

  82. Enhanced antibacterial activity, corrosion resistance and endothelialization potential of Ti-5Cu alloy by oxygen and nitrogen plasma-based surface modification 

  83. Upregulation of SEMP1 Contributes to Improving the Biological Functions of Trophoblast via the PI3K/AKT Pathway in Preeclampsia 

  84. Exosomal B7-H3 facilitates colorectal cancer angiogenesis and metastasis through AKT1/mTOR/VEGFA pathway 

  85. P-Hydroxylcinnamaldehyde induces tumor-associated macrophage polarization toward the M1 type by regulating the proteome and inhibits ESCC in vivo and in vitro 

  86. Resveratrol-induced SIRT1 activation inhibits glycolysis-fueled angiogenesis under rheumatoid arthritis conditions independent of HIF-1α 

  87. FOXN3 inhibits the progression of ovarian cancer through negatively regulating the expression of RPS15A 

  88. Nanotubular TiO2 regulates macrophage M2 polarization and increases macrophage secretion of VEGF to accelerate endothelialization via the ERK1/2 and PI3K/AKT pathways 

  89. 3D-bioprinted double-crosslinked angiogenic alginate/chondroitin sulfate patch for diabetic wound healing 

  90. Duo-role Platelet-rich Plasma: temperature-induced fibrin gel and growth factors’ reservoir for microneedles to promote hair regrowth 

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  92. 细胞培养上清

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  93. Tanshinone Iia Inhibits Β-Catenin/Vegf-Mediated Angiogenesis by Targeting Tgf-Β1 in Normoxic and Hif-1α?in Hypoxic Microenvironments in Human Colorectal Cancer 

  94. B7-H3 Expression in Breast Cancer and Upregulation of Vegf through Gene Silence 

  95. Tanshinone I Inhibits Tumor Angiogenesis by Reducing Stat3 Phosphorylation at Tyr705 and Hypoxia-Induced Hif-1α Accumulation in Both Endothelial and Tumor Cells 

  96. Levels of Erythropoietin and Vascular Endothelial Growth Factor in Surgery-Required Advanced Neovascular Glaucoma Eyes before and after Intravitreal Injection of Bevacizumab 

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