EK190

Human sVCAM-1/CD106 ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:VCAM1, CD106
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:100 μL预稀释样本
  • 灵敏度:2.65 pg/mL
  • 检测范围:31.25 pg/mL - 2000 pg/mL
  • 回收率:86% - 111%

在售SKU:70-EK190-48, 70-EK190-96


描述

商品名

Human sVCAM-1/CD106 ELISA Kit (人血管细胞粘附分子1 (VCAM-1) ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:1.9% - 2.6%;板间变异系数:3.2% - 5.0%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

100 μL预稀释样本

灵敏度

2.65 pg/mL

检测范围

31.25 pg/mL - 2000 pg/mL

回收率

86% - 111%

平均回收率

0.99

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗sVCAM-1/CD106单克隆抗体的96孔聚苯乙烯酶标板
  • 人sVCAM-1/CD106冻干标准品
  • sVCAM-1/CD106检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人sVCAM-1抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的sVCAM-1与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中sVCAM-1的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human sVCAM-1/CD106 ELISA Kit (人血管细胞粘附分子1 (VCAM-1) ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 VCAM1, CD106 靶点信息

VCAM1 分子靶点信息概述

  • 分子名:VCAM1, vascular cell adhesion molecule 1
  • 基因家族:CD molecules; Receptor ligands; C2-set domain containing; I-set domain containing; Ig-like cell adhesion molecule family
  • 别名:CD106

VCAM1 分子靶点综述

血管细胞粘附分子1(VCAM-1)也称为CD106,是100-110kDaI型跨膜蛋白,通常具有7个C2型免疫球蛋白结构域,作为细胞黏附分子发挥功能。VCAM-1蛋白介导淋巴细胞、单核细胞、嗜酸性粒细胞和嗜碱性粒细胞等黏附于血管内皮。VCAM-1是整合素β1亚家族VLA-4(极迟抗原-4或α4β1)和整合素α4β7的内皮配体。在其他类型细胞上也观察到VCAM-1的表达。它也能与EZR和膜突蛋白作用。VCAM-1的其它功能包括通过细胞-细胞接触调控破骨细胞生成、血氧不足时诱导镰状细胞黏附于血管内皮细胞。它还在白细胞-内皮细胞信号传导中发挥作用。监测血清中的sVCAM-1表达水平可为多种病理状态提供更详细的视角,如动脉粥样硬化和类风湿性关节炎。

人 Human VCAM1 分子靶点信息

  • 分子名:VCAM1, vascular cell adhesion molecule 1
  • 别称:
    • CD106
    • CD106 antigen
    • DKFZp779G2333
    • INCAM-100
    • MGC99561
    • vascular cell adhesion protein 1
  • 基因序列:NCBI_Gene: 7412
  • 蛋白序列:UniProtKB: P19320

人 Human VCAM1靶点分子功能(预测)

Enables integrin binding activity and primary amine oxidase activity. Involved in several processes, including calcium-mediated signaling using intracellular calcium source; cell-cell adhesion in response to extracellular stimulus; and cellular response to amyloid-beta. Located in several cellular components, including early endosome; external side of plasma membrane; and podosome. Part of alpha9-beta1 integrin-vascular cell adhesion molecule-1 complex. Biomarker of several diseases, including artery disease (multiple); diabetes mellitus (multiple); essential thrombocythemia; glomerulonephritis (multiple); and interstitial cystitis.

操作步骤

ELISA操作常见问题

查看更多ELISA操作相关问题

引用文献


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  2. Inhibition of Matrix Metalloproteinase-8 Protects Against Sepsis Serum Mediated Leukocyte Adhesion 

  3. Plasma exosomal miRNAs involved in endothelial injury in microscopic polyangiitis patients 

  4. Involvement of activation of C-met signaling pathway in CD151-induced HUVECs angiogenesis 

  5. Nesfatin-1 inhibits free fatty acid (FFA)-induced endothelial inflammation via Gfi1/NF-κB signaling 

  6. Procyanidin B2 alleviates oxidized low-density lipoprotein-induced cell injury, inflammation, monocyte chemotaxis, and oxidative stress by inhibiting the nuclear factor kappa-B pathway in human umbilical vein endothelial cells 

  7. LncRNA MALAT1 suppresses monocyte-endothelial cell interactions by targeting miR-30b-5p and enhancing ATG5-mediated autophagy 

  8. A dual-targeting therapeutic nanobubble for imaging-guided atherosclerosis treatment 

  9. Long-term abuse of caffeine sodium benzoate induces endothelial cells injury and leads to coagulation dysfunction 

  10. ATP-citrate lyase controls endothelial gluco-lipogenic metabolism and vascular inflammation in sepsis-associated organ injury 

  11. 5-epi-ent-Kaurane diterpenoids from the aerial parts of Isodon eriocalyx and their anti-atherosclerotic potential 

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