Human sVCAM-1/CD106 ELISA Kit检测试剂盒（酶联免疫吸附法）
¥1,600.00 – ¥2,650.00
因产品会迭代升级，具体实验步骤请按纸质版说明书操作
- 分子靶点：VCAM1, CD106
- 种属：人 (Human)
- 样本类型：血清，血浆，细胞培养上清及其他生物学样本
- 检测样本体积：100 μL预稀释样本
- 灵敏度：2.65 pg/mL
- 检测范围：31.25 pg/mL - 2000 pg/mL
- 回收率：86% - 111%
在售SKU:70-EK190-48, 70-EK190-96
描述
| 商品名 |
Human sVCAM-1/CD106 ELISA Kit (人血管细胞粘附分子1 (VCAM-1) ELISA试剂盒) |
|---|---|
| 检测方法 |
双抗夹心法 |
| 精密度 |
板内变异系数：1.9% - 2.6%；板间变异系数：3.2% - 5.0% |
| 样本类型 |
血清，血浆，细胞培养上清及其他生物学样本 |
| 检测样本体积 |
100 μL预稀释样本 |
| 灵敏度 |
2.65 pg/mL |
| 检测范围 |
31.25 pg/mL - 2000 pg/mL |
| 回收率 |
86% - 111% |
| 平均回收率 |
0.99 |
| 板式 |
96孔板，可拆 |
| 保存 |
试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。 |
| 运输条件 |
4℃蓝冰运输 |
| 组分 |
|
检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人sVCAM-1抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的sVCAM-1与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中sVCAM-1的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
VCAM1 分子靶点信息概述
- 分子名：VCAM1, vascular cell adhesion molecule 1
- 基因家族：CD molecules; Receptor ligands; C2-set domain containing; I-set domain containing; Ig-like cell adhesion molecule family
- 别名：CD106
VCAM1 分子靶点综述
血管细胞粘附分子1(VCAM-1)也称为CD106，是100-110kDaI型跨膜蛋白，通常具有7个C2型免疫球蛋白结构域，作为细胞黏附分子发挥功能。VCAM-1蛋白介导淋巴细胞、单核细胞、嗜酸性粒细胞和嗜碱性粒细胞等黏附于血管内皮。VCAM-1是整合素β1亚家族VLA-4(极迟抗原-4或α4β1)和整合素α4β7的内皮配体。在其他类型细胞上也观察到VCAM-1的表达。它也能与EZR和膜突蛋白作用。VCAM-1的其它功能包括通过细胞-细胞接触调控破骨细胞生成、血氧不足时诱导镰状细胞黏附于血管内皮细胞。它还在白细胞-内皮细胞信号传导中发挥作用。监测血清中的sVCAM-1表达水平可为多种病理状态提供更详细的视角，如动脉粥样硬化和类风湿性关节炎。
人 Human VCAM1 分子靶点信息
- 分子名：VCAM1, vascular cell adhesion molecule 1
- 别称：
- CD106
- CD106 antigen
- DKFZp779G2333
- INCAM-100
- MGC99561
- vascular cell adhesion protein 1
- 基因序列：NCBI_Gene: 7412
- 蛋白序列：UniProtKB: P19320
人 Human VCAM1靶点分子功能（预测）
Enables integrin binding activity and primary amine oxidase activity. Involved in several processes, including calcium-mediated signaling using intracellular calcium source; cell-cell adhesion in response to extracellular stimulus; and cellular response to amyloid-beta. Located in several cellular components, including early endosome; external side of plasma membrane; and podosome. Part of alpha9-beta1 integrin-vascular cell adhesion molecule-1 complex. Biomarker of several diseases, including artery disease (multiple); diabetes mellitus (multiple); essential thrombocythemia; glomerulonephritis (multiple); and interstitial cystitis.
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
- 血清OR血浆，哪个是ELISA的菜
- ELISA通关必备丨数据篇丨标准曲线不佳
- ELISA通关必备丨操作篇丨常见问题及解决方案
- ELISA通关必备丨操作篇丨溶解与稀释标准品
- ELISA通关必备丨样本篇丨不常见样本
- ELISA通关必备丨样本篇丨常见样本丨细胞
- ELISA通关必备丨如何选择试剂盒
- ELISA通关必备丨基础知识
- 真？假？ELISA试剂盒选择要小心
- ELISA常见类型一 | 双抗夹心法，你要的都在这里！
- ELISA常见类型二 | 竞争法，五分钟搞定！
- 叮！联科向您投递了个ELISA实验操作干货包，请查收~
- 【视频】ELISA实验操作步骤演示视频教程
- ELISA 组织样本的处理—大鼠组织
- 【视频】ELISA实验原理与常见问题分析
- 查看更多ELISA操作相关问题
ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
文章目录[隐藏]
- Metabolic Profiling in Association with Vascular Endothelial Cell Dysfunction Following Non-Toxic Cadmium Exposure
- Inhibition of Matrix Metalloproteinase-8 Protects Against Sepsis Serum Mediated Leukocyte Adhesion
- Plasma exosomal miRNAs involved in endothelial injury in microscopic polyangiitis patients
- Involvement of activation of C-met signaling pathway in CD151-induced HUVECs angiogenesis
- Nesfatin-1 inhibits free fatty acid (FFA)-induced endothelial inflammation via Gfi1/NF-κB signaling
- Procyanidin B2 alleviates oxidized low-density lipoprotein-induced cell injury, inflammation, monocyte chemotaxis, and oxidative stress by inhibiting the nuclear factor kappa-B pathway in human umbilical vein endothelial cells
- LncRNA MALAT1 suppresses monocyte-endothelial cell interactions by targeting miR-30b-5p and enhancing ATG5-mediated autophagy
- A dual-targeting therapeutic nanobubble for imaging-guided atherosclerosis treatment
- Long-term abuse of caffeine sodium benzoate induces endothelial cells injury and leads to coagulation dysfunction
- ATP-citrate lyase controls endothelial gluco-lipogenic metabolism and vascular inflammation in sepsis-associated organ injury
- 5-epi-ent-Kaurane diterpenoids from the aerial parts of Isodon eriocalyx and their anti-atherosclerotic potential
Metabolic Profiling in Association with Vascular Endothelial Cell Dysfunction Following Non-Toxic Cadmium Exposure
Inhibition of Matrix Metalloproteinase-8 Protects Against Sepsis Serum Mediated Leukocyte Adhesion
Plasma exosomal miRNAs involved in endothelial injury in microscopic polyangiitis patients
Involvement of activation of C-met signaling pathway in CD151-induced HUVECs angiogenesis
Nesfatin-1 inhibits free fatty acid (FFA)-induced endothelial inflammation via Gfi1/NF-κB signaling
Procyanidin B2 alleviates oxidized low-density lipoprotein-induced cell injury, inflammation, monocyte chemotaxis, and oxidative stress by inhibiting the nuclear factor kappa-B pathway in human umbilical vein endothelial cells
LncRNA MALAT1 suppresses monocyte-endothelial cell interactions by targeting miR-30b-5p and enhancing ATG5-mediated autophagy
A dual-targeting therapeutic nanobubble for imaging-guided atherosclerosis treatment
Long-term abuse of caffeine sodium benzoate induces endothelial cells injury and leads to coagulation dysfunction
ATP-citrate lyase controls endothelial gluco-lipogenic metabolism and vascular inflammation in sepsis-associated organ injury
5-epi-ent-Kaurane diterpenoids from the aerial parts of Isodon eriocalyx and their anti-atherosclerotic potential
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