EK189

Human sICAM-1/CD54 ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：ICAM1, BB2, CD54
  • 种属：人 (Human)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：50 μL
  • 灵敏度：3.81 pg/mL
  • 检测范围：31.25 pg/mL - 2000 pg/mL
  • 回收率：99% - 118%

在售SKU:70-EK189-48, 70-EK189-96

描述

商品名

Human sICAM-1/CD54 ELISA Kit (人细胞间粘附分子1 (ICAM-1) ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：4.9%-6.7%；板间变异系数：6.3% - 7.5%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

50 μL
灵敏度

3.81 pg/mL
检测范围

31.25 pg/mL - 2000 pg/mL
回收率

99% - 118%
平均回收率

1.1
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗sICAM-1/CD54单克隆抗体的96孔聚苯乙烯酶标板
  • 人sICAM-1/CD54冻干标准品
  • sICAM-1/CD54检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人sICAM-1抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的sICAM-1与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中sICAM-1的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human sICAM-1/CD54 ELISA Kit (人细胞间粘附分子1 (ICAM-1) ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 ICAM1, BB2, CD54 靶点信息

ICAM1 分子靶点信息概述

  • 分子名：ICAM1, intercellular adhesion molecule 1
  • 基因家族：CD molecules; Receptor ligands; Immunoglobulin like domain containing; Ig-like cell adhesion molecule family
  • 别名：BB2; CD54
  • 全称：human rhinovirus receptor

ICAM1 分子靶点综述

细胞间粘附分子1(ICAM-1)也称为CD54，是一个几乎无所不在的跨膜糖蛋白，通常表达于内皮细胞和免疫细胞，对白细胞迁移和活化具有关键作用。它与CD11a/CD18或CD11b/CD18型整合素结合，也会被鼻病毒作为受体利用。可溶性细胞间粘附分子1(sICAM-1)促进血管生成，可作为血管内皮细胞活化或受损的指示剂。它也可作为跨膜ICAM-1抑制剂，介导活性如单核细胞对活化的内皮细胞的黏附以及肿瘤细胞对NK细胞介导的溶解作用的敏感性。监测血清中的sICAM-1表达水平可对多种病理状态提供更加详细的视角，如心血管疾病、II型糖尿病、器官移植障碍、氧化应激、腹部脂肪团、高血压、肝脏疾病和某些恶性肿瘤等。

人 Human ICAM1 分子靶点信息

  • 分子名：ICAM1, intercellular adhesion molecule 1
  • 别称：
    • BB2
    • CD54
    • cell surface glycoprotein P3.58
    • human rhinovirus receptor
    • ICAM-1
    • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
    • major group rhinovirus receptor
    • P3.58
  • 基因序列：NCBI_Gene: 3383
  • 蛋白序列：UniProtKB: P05362

人 Human ICAM1靶点分子功能（预测）

Enables integrin binding activity. Involved in several processes, including T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; cellular response to amyloid-beta; and negative regulation of apoptotic process. Located in extracellular exosome. Implicated in several diseases, including autoimmune disease (multiple); biliary atresia; diabetic retinopathy; inflammatory bowel disease (multiple); and liver cirrhosis. Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); diabetes mellitus (multiple); hematologic cancer (multiple); and lung disease (multiple).

操作步骤


下载本产品说明书

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

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