EK9137

Human/Rat PDGF-BB ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

分子靶点：待定
种属：人 (Human); 大鼠 (Rat)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：100 μL
灵敏度：2.13 pg/mL
检测范围：31.25 pg/mL - 2000 pg/mL
回收率：82% - 116%

在售SKU:70-EK9137-48, 70-EK9137-96

描述

商品名	Human/Rat PDGF-BB ELISA Kit (人/大鼠PDGF-BB ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：2.3% - 4.5%；板间变异系数：3.3% - 4.9%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	100 μL
灵敏度	2.13 pg/mL
检测范围	31.25 pg/mL - 2000 pg/mL
回收率	82% - 116%
平均回收率	0.95
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗PDGF-BB单克隆抗体的96孔聚苯乙烯酶标板人/大鼠PDGF-BB冻干标准品 PDGF-BB检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人/大鼠PDGF-BB抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的PDGF-BB与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中PDGF-BB的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human/Rat PDGF-BB ELISA Kit (人/大鼠PDGF-BB ELISA试剂盒) - 标准曲线 — 标准曲线

操作步骤

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引用文献

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The conditioned medium from mesenchymal stromal cells pretreated with proinflammatory cytokines promote fibroblasts migration and activation
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Duo-role Platelet-rich Plasma: temperature-induced fibrin gel and growth factors’ reservoir for microneedles to promote hair regrowth
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