EK9137

Human/Rat PDGF-BB ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

分子靶点：待定
种属：人 (Human); 大鼠 (Rat)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：100 μL
灵敏度：2.13 pg/mL
检测范围：31.25 pg/mL - 2000 pg/mL
回收率：82% - 116%

在售SKU:70-EK9137-48, 70-EK9137-96

描述

商品名	Human/Rat PDGF-BB ELISA Kit (人/大鼠PDGF-BB ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：2.3% - 4.5%；板间变异系数：3.3% - 4.9%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	100 μL
灵敏度	2.13 pg/mL
检测范围	31.25 pg/mL - 2000 pg/mL
回收率	82% - 116%
平均回收率	0.95
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗PDGF-BB单克隆抗体的96孔聚苯乙烯酶标板人/大鼠PDGF-BB冻干标准品 PDGF-BB检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人/大鼠PDGF-BB抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的PDGF-BB与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中PDGF-BB的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human/Rat PDGF-BB ELISA Kit (人/大鼠PDGF-BB ELISA试剂盒) - 标准曲线 — 标准曲线

操作步骤

文章目录[隐藏]

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引用文献

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The conditioned medium from mesenchymal stromal cells pretreated with proinflammatory cytokines promote fibroblasts migration and activation
The phenotype of vascular smooth muscle cells co-cultured with endothelial cells is modulated by PDGFR-β/IQGAP1 signaling in LPS-induced intravascular injury
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Macrophage Polarization Mediated by Chitooligosaccharide (COS) and Associated Osteogenic and Angiogenic Activities
Advanced-platelet-rich fibrin extract promotes adipogenic and osteogenic differentiation of human adipose-derived stem cells in a dose-dependent manner in vitro
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IRF1/ZNF350/GPX4-mediated ferroptosis of renal tubular epithelial cells promote chronic renal allograft interstitial fibrosis
Ferroptosis contributes to endometrial fibrosis in intrauterine adhesions
The Role of Macrophage Phenotype in the Vascularization of Prevascularized Human Bone Marrow Mesenchymal Stem Cell Sheets
Duo-role Platelet-rich Plasma: temperature-induced fibrin gel and growth factors’ reservoir for microneedles to promote hair regrowth
Proximity Hybridization-Induced on Particle DNA Walker for Ultrasensitive Protein Detection
Simvastatin Preparations Promote Pdgf-Bb Secretion to Repair Lps-Induced Endothelial Injury through the Pdgfrβ/Pi3k/Akt/Iqgap1 Signalling Pathway

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