EK1213S
Human PDGF-AA Standard (人血小板衍生因子 (PDGF) 标准品)
¥580.00
因产品会迭代升级，具体实验步骤请按纸质版说明书操作
描述
本产品只包含标准品试剂，如需购买试剂盒请点击下图
-
- EK1213
- ELISA试剂盒
Human PDGF-AA ELISA Kit检测试剂盒（酶联免疫吸附法）
- ¥1,600.00 – ¥2,650.00
| 商品名 |
Human PDGF-AA Standard (人血小板衍生因子 (PDGF) 标准品) |
|---|---|
| 组分 |
人PDGF-AA 标准品 |
| 检测方法 |
双抗夹心法 |
| 样本类型 |
血清，血浆，细胞培养上清及其他生物学样本 |
| 板式 |
管 |
| 保存 |
短期4℃，长期-20℃保存 |
| 运输条件 |
4℃蓝冰运输 |
检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人PDGF-AA抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的PDGF-AA与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中PDGF-AA的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
- 血清OR血浆，哪个是ELISA的菜
- ELISA通关必备丨数据篇丨标准曲线不佳
- ELISA通关必备丨操作篇丨常见问题及解决方案
- ELISA通关必备丨操作篇丨溶解与稀释标准品
- ELISA通关必备丨样本篇丨不常见样本
- ELISA通关必备丨样本篇丨常见样本丨细胞
- ELISA通关必备丨如何选择试剂盒
- ELISA通关必备丨基础知识
- 真？假？ELISA试剂盒选择要小心
- ELISA常见类型一 | 双抗夹心法，你要的都在这里！
- ELISA常见类型二 | 竞争法，五分钟搞定！
- 叮！联科向您投递了个ELISA实验操作干货包，请查收~
- 【视频】ELISA实验操作步骤演示视频教程
- ELISA 组织样本的处理—大鼠组织
- 【视频】ELISA实验原理与常见问题分析
- 查看更多ELISA操作相关问题
ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
文章目录[隐藏]
- Liproxstatin-1 attenuates unilateral ureteral obstruction-induced renal fibrosis by inhibiting renal tubular epithelial cells ferroptosis
- Growth factors-based platelet lysate rejuvenates skin against ageing through NF-κB signalling pathway: In vitro and in vivo mechanistic and clinical studies
- Utilizing 3D bioprinted platelet-rich fibrin-based materials to promote the regeneration of oral soft tissue
- Growth factors-based beneficial effects of platelet lysate on umbilical cord-derived stem cells and their synergistic use in osteoarthritis treatment
- FASN promotes lymph node metastasis in cervical cancer via cholesterol reprogramming and lymphangiogenesis
- Andrographolide suppresses breast cancer progression by modulating tumor-associated macrophage polarization through the Wnt/β-catenin pathway
- Comparison of the efficacy and safety of warfarin anticoagulation and left atrial appendage transcatheter occlusion in the treatment of non-valvular atrial fibrillation and investigation of ET-1, hs-CRP and PDGFs
- Tumor-associated macrophages drive glycolysis through the IL-8/STAT3/GLUT3 signaling pathway in pancreatic cancer progression
Liproxstatin-1 attenuates unilateral ureteral obstruction-induced renal fibrosis by inhibiting renal tubular epithelial cells ferroptosis
Growth factors-based platelet lysate rejuvenates skin against ageing through NF-κB signalling pathway: In vitro and in vivo mechanistic and clinical studies
Utilizing 3D bioprinted platelet-rich fibrin-based materials to promote the regeneration of oral soft tissue
Growth factors-based beneficial effects of platelet lysate on umbilical cord-derived stem cells and their synergistic use in osteoarthritis treatment
FASN promotes lymph node metastasis in cervical cancer via cholesterol reprogramming and lymphangiogenesis
Andrographolide suppresses breast cancer progression by modulating tumor-associated macrophage polarization through the Wnt/β-catenin pathway
Comparison of the efficacy and safety of warfarin anticoagulation and left atrial appendage transcatheter occlusion in the treatment of non-valvular atrial fibrillation and investigation of ET-1, hs-CRP and PDGFs
Tumor-associated macrophages drive glycolysis through the IL-8/STAT3/GLUT3 signaling pathway in pancreatic cancer progression
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