EK1213
Human PDGF-AA ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
在售SKU:70-EK1213-48, 70-EK1213-96, EK1213
- 种属:人
- 样本类型:血清、血浆、细胞培养上清及其他生物学样本
- 检测样本体积:血清、血浆样本10μL
- 灵敏度:2.47pg/mL
- 检测范围:15.63-1000pg/mL
- 回收率:90%-110%
ELISA试剂盒详细信息
商品名 | 人血小板衍生因子AA酶联免疫检测试剂盒 |
---|---|
种属 | 人 |
检测方法 | 双抗体夹心法 |
检测样本类型 | 血清、血浆、细胞培养上清及其他生物学样本 |
检测样本体积 | 血清、血浆样本10μL |
灵敏度 | 2.47pg/mL |
线性范围 | 15.63-1000pg/mL |
精密度 | 板内变异系数:3.4%-4.9%;板间变异系数:3.5%-4.5% |
回收率 | 90%-110% |
平均回收率 | 101% |
板式 | 96孔板,可拆 |
保存条件 | 2-8℃保存。已拆开:标准品-20℃保存,其它4℃。 |
运输条件 | 2-8℃冰袋运输 |
检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人PDGF-AA抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的PDGF-AA与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中PDGF-AA的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。 |
引用文献统计
该产品被引用的文献总数为:8
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