Human P4HB Standard (人脯氨酸4-羟化酶β亚基 (P4HB) 标准品)
¥280.00
描述
文章目录[隐藏]
本产品只包含标准品试剂,如需购买试剂盒请点击下图
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- EK1368
- ELISA试剂盒
Human P4HB ELISA Kit检测试剂盒(酶联免疫吸附法)
- ¥2,000.00 – ¥3,400.00
商品名 |
Human P4HB Standard (人脯氨酸4-羟化酶β亚基 (P4HB) 标准品) |
---|---|
组分 |
人P4HB冻干标准品 |
检测方法 |
双抗夹心法 |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
板式 |
管 |
保存 |
短期4℃,长期-20℃保存 |
运输条件 |
4℃蓝冰运输 |
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人P4HB预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和辣根过氧化物酶标记的检测抗体,经过孵育,样本中存在的P4HB与固相抗体和检测抗体结合。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中P4HB的浓度成正比。加入终止液终止反应,在450 nm波长 (参考波长570 - 630 nm)测定吸光度值。
![Human P4HB ELISA Kit (人脯氨酸4-羟化酶β亚基 (P4HB) ELISA试剂盒) - 标准曲线](https://www.liankebio.com/wp-content/uploads/EK1368-01.png)
分子信息
P4HB 分子靶点信息概述
- 分子名:P4HB, prolyl 4-hydroxylase subunit beta
- 基因家族:Protein disulfide isomerases
- 别名:PDIA1; PROHB; DSI; GIT; PDI; PO4HB; P4Hbeta
- 曾用名:PO4DB; ERBA2L
- 全称:protein disulfide isomerase-associated 1; protein disulfide isomerase family A, member 1; collagen prolyl 4-hydroxylase beta; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide (protein disulfide isomerase; thyroid hormone binding protein p55); procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide (protein disulfide isomerase-associated 1); procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide; prolyl 4-hydroxylase, beta polypeptide
P4HB 分子靶点综述
蛋白质二硫键异构酶,又称脯氨酸4-羟化酶β亚基(P4HB)、细胞甲状腺激素结合蛋白(p55)和谷胱甘肽-胰岛素转氢酶,是一种外周膜蛋白,属于蛋白二硫键异构酶家族。它包含两个硫氧还蛋白结构域,催化二硫键的形成、断裂和重排。P4HB定位于淋巴细胞表面CD4富集区附近。在黑素小体组分中,P4HB可降低并激活HIV-1 gp120表面蛋白的融合特性,从而使HIV-1进入细胞。P4HB作为一种伴侣,抑制错误折叠蛋白的聚集。P4HB可能与其它分子伴侣参与了激素生物合成中TG前体的结构修饰。
人 Human P4HB 分子靶点信息
- 分子名:P4HB, prolyl 4-hydroxylase subunit beta
- 别称:
- cellular thyroid hormone-binding protein
- CLCRP1
- collagen prolyl 4-hydroxylase beta
- DSI
- ERBA2L
- GIT
- glutathione-insulin transhydrogenase
- P4Hbeta
- p55
- PDI
- PDIA1
- PHDB
- PO4DB
- PO4HB
- procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide
- PROHB
- prolyl 4-hydroxylase, beta polypeptide
- protein disulfide isomerase family A, member 1
- protein disulfide isomerase-associated 1
- protein disulfide isomerase/oxidoreductase
- protein disulfide-isomerase
- protocollagen hydroxylase
- testicular secretory protein Li 32
- thyroid hormone-binding protein p55
- v-erb-a avian erythroblastic leukemia viral oncogene homolog 2-like
- 基因序列:NCBI_Gene: 5034
- 蛋白序列:UniProtKB: P07237
人 Human P4HB靶点分子功能(预测)
Enables several functions, including oxidoreductase activity, acting on a sulfur group of donors; protein disulfide isomerase activity; and protein heterodimerization activity. Contributes to procollagen-proline 4-dioxygenase activity. Involved in several processes, including positive regulation of substrate adhesion-dependent cell spreading; positive regulation of viral entry into host cell; and protein folding in endoplasmic reticulum. Acts upstream of or within peptidyl-proline hydroxylation to 4-hydroxy-L-proline. Located in several cellular components, including endoplasmic reticulum-Golgi intermediate compartment; external side of plasma membrane; and lamellipodium. Part of procollagen-proline 4-dioxygenase complex. Implicated in Cole-Carpenter syndrome.
操作步骤
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