EK9162

Human/Mouse/Rat TGF-β2 ELISA Kit检测试剂盒（酶联免疫吸附法）

X

¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：TGFB2, TGB-β2
  • 种属：人 (Human), Mouse(小鼠), Rat(大鼠)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：100 μL
  • 灵敏度：0.33 pg/mL
  • 检测范围：31.25 pg/mL - 2000 pg/mL
  • 回收率：88% - 120%

在售SKU:70-EK9162-48, 70-EK9162-96

描述

商品名

Human/Mouse/Rat TGF-β2 ELISA Kit (人/小鼠/大鼠转化生长因子β2 (TGF-β2) ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：2.7% - 4.5%；板间变异系数：3.3% - 4.8%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

100 μL
灵敏度

0.33 pg/mL
检测范围

31.25 pg/mL - 2000 pg/mL
回收率

88% - 120%
平均回收率

1.07
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗TGF-β2单克隆抗体的96孔聚苯乙烯酶标板
  • 人/小鼠/大鼠TGF-β2冻干标准品
  • TGF-β2检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人/小鼠/大鼠TGF-β2抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的TGF-β2与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中TGF-β2的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human/Mouse/Rat TGF-β2 ELISA Kit (人/小鼠/大鼠转化生长因子β2 (TGF-β2) ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 TGFB2, TGB-β2 靶点信息

TGFB2 分子靶点信息概述

  • 分子名：TGFB2, transforming growth factor beta 2
  • 基因家族：Transforming growth factor beta family
  • 全称：prepro-transforming growth factor beta-2; transforming growth factor, beta 2

TGFB2 分子靶点综述

转化生长因子β2(TGF-β2)前体蛋白由19个氨基酸的信号肽、283个氨基酸的前区和112个氨基酸的成熟区组成。人TGF-β2成熟区与人TGF-β1和TGF-β3的同源性分别为71%和80%，与小鼠TGF-β2的同源性为97%。它可由各类细胞表达，包括破骨细胞、胸腺上皮细胞、角蛋白细胞、肝细胞、胃的主细胞和卫星细胞等。TGF-β2具有显著的种属交叉生物活性，如人TGF-β2对小鼠细胞具有活性，而猪TGF-β2对兔细胞具有活性等。TGF-β2具有4种基本活性：是大部分类型细胞的生长抑制因子；可增强细胞外基质的沉积；具有免疫抑制作用，抑制抗原递呈细胞表达IL-12和CD40L，上调IL-10分泌；在胚胎发育期表达于离散区域，如上皮、心肌、手足的软骨和骨骼、以及神经系统，提示其具有特异性的功能。

人 Human TGFB2 分子靶点信息

  • 分子名：TGFB2, transforming growth factor beta 2
  • 别称：
    • BSC-1 cell growth inhibitor
    • cetermin
    • G-TSF
    • glioblastoma-derived T-cell suppressor factor
    • LDS4
    • MGC116892
    • polyergin
    • prepro-transforming growth factor beta-2
    • TGF-beta-2
    • TGF-beta2
    • transforming growth factor beta-2
    • transforming growth factor beta-2 proprotein
    • transforming growth factor, beta 2
  • 基因序列：NCBI_Gene: 7042
  • 蛋白序列：UniProtKB: P61812

人 Human TGFB2靶点分子功能（预测）

Enables amyloid-beta binding activity; protein homodimerization activity; and transforming growth factor beta receptor binding activity. Involved in several processes, including animal organ development; cell surface receptor signaling pathway; and positive regulation of signal transduction. Located in collagen-containing extracellular matrix and extracellular space. Implicated in Loeys-Dietz syndrome 4 and colorectal cancer. Biomarker of colon carcinoma; colorectal cancer; and liver cirrhosis.

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


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  5. S-allylmercaptocysteine inhibits mucin overexpression and inflammation via MAPKs and PI3K-Akt signaling pathways in acute respiratory distress syndrome 

  6. Enhancing anti-tumor efficacy and immune memory by combining 3p-GPC-3 siRNA treatment with PD-1 blockade in hepatocellular carcinoma 

  7. Involvement of MM cell‑derived exosomes in T lymphocytes immune responses 

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  11. Peritoneal metastatic gastric carcinoma cells exhibit more malignant behavior when co-cultured with HMrSV5 cells 

  12. Tumor-associated macrophages promote pancreatic ductal adenocarcinoma progression by inducing epithelial-to-mesenchymal transition 

  13. Hyperbaric Oxygen Improves the Survival and Angiogenesis of Fat Grafts after Autologous Fat Transplantation 

  14. ROS-responsive liposomes as an inhaled drug delivery nanoplatform for idiopathic pulmonary fibrosis treatment via Nrf2 signaling 

  15. HOXA10 mediates epithelial-mesenchymal transition to promote gastric cancer metastasis partly via modulation of TGFB2/Smad/METTL3 signaling axis 

  16. Chondroprotective effects of platelet lysate towards monoiodoacetate-induced arthritis by suppression of TNF-α-induced activation of NF-ĸB pathway in chondrocytes 

  17. Bortezomib alleviates antibody-mediated rejection in kidney transplantation by facilitating Atg5 expression 

  18. Silica dust exposure induces pulmonary fibrosis through autophagy signaling 

  19. Plantaginis Semen polysaccharides ameliorate renal damage through regulating NLRP3 inflammasome in gouty nephropathy rats 

  20. Bone marrow mesenchymal stem cells modified with heme oxygenase-1 alleviate rejection of donation after circulatory death liver transplantation by inhibiting dendritic cell maturation in rats 

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  51. Perdurable PD-1 blockage awakes anti-tumor immunity suppressed by precise chemotherapy 

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