EK9162

Human/Mouse/Rat TGF-β2 ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：TGFB2, TGB-β2
  • 种属：人 (Human), Mouse(小鼠), Rat(大鼠)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：100 μL
  • 灵敏度：0.33 pg/mL
  • 检测范围：31.25 pg/mL - 2000 pg/mL
  • 回收率：88% - 120%

在售SKU:70-EK9162-48, 70-EK9162-96

描述

商品名

Human/Mouse/Rat TGF-β2 ELISA Kit (人/小鼠/大鼠转化生长因子β2 (TGF-β2) ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：2.7% - 4.5%；板间变异系数：3.3% - 4.8%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

100 μL
灵敏度

0.33 pg/mL
检测范围

31.25 pg/mL - 2000 pg/mL
回收率

88% - 120%
平均回收率

1.07
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗TGF-β2单克隆抗体的96孔聚苯乙烯酶标板
  • 人/小鼠/大鼠TGF-β2冻干标准品
  • TGF-β2检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人/小鼠/大鼠TGF-β2抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的TGF-β2与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中TGF-β2的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human/Mouse/Rat TGF-β2 ELISA Kit (人/小鼠/大鼠转化生长因子β2 (TGF-β2) ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 TGFB2, TGB-β2 靶点信息

TGFB2 分子靶点信息概述

  • 分子名：TGFB2, transforming growth factor beta 2
  • 基因家族：Transforming growth factor beta family
  • 全称：prepro-transforming growth factor beta-2; transforming growth factor, beta 2

TGFB2 分子靶点综述

转化生长因子β2(TGF-β2)前体蛋白由19个氨基酸的信号肽、283个氨基酸的前区和112个氨基酸的成熟区组成。人TGF-β2成熟区与人TGF-β1和TGF-β3的同源性分别为71%和80%，与小鼠TGF-β2的同源性为97%。它可由各类细胞表达，包括破骨细胞、胸腺上皮细胞、角蛋白细胞、肝细胞、胃的主细胞和卫星细胞等。TGF-β2具有显著的种属交叉生物活性，如人TGF-β2对小鼠细胞具有活性，而猪TGF-β2对兔细胞具有活性等。TGF-β2具有4种基本活性：是大部分类型细胞的生长抑制因子；可增强细胞外基质的沉积；具有免疫抑制作用，抑制抗原递呈细胞表达IL-12和CD40L，上调IL-10分泌；在胚胎发育期表达于离散区域，如上皮、心肌、手足的软骨和骨骼、以及神经系统，提示其具有特异性的功能。

人 Human TGFB2 分子靶点信息

  • 分子名：TGFB2, transforming growth factor beta 2
  • 别称：
    • BSC-1 cell growth inhibitor
    • cetermin
    • G-TSF
    • glioblastoma-derived T-cell suppressor factor
    • LDS4
    • MGC116892
    • polyergin
    • prepro-transforming growth factor beta-2
    • TGF-beta-2
    • TGF-beta2
    • transforming growth factor beta-2
    • transforming growth factor beta-2 proprotein
    • transforming growth factor, beta 2
  • 基因序列：NCBI_Gene: 7042
  • 蛋白序列：UniProtKB: P61812

人 Human TGFB2靶点分子功能（预测）

Enables amyloid-beta binding activity; protein homodimerization activity; and transforming growth factor beta receptor binding activity. Involved in several processes, including animal organ development; cell surface receptor signaling pathway; and positive regulation of signal transduction. Located in collagen-containing extracellular matrix and extracellular space. Implicated in Loeys-Dietz syndrome 4 and colorectal cancer. Biomarker of colon carcinoma; colorectal cancer; and liver cirrhosis.

操作步骤


下载本产品说明书

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

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引用文献

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