EK981

Human/Mouse TGF-β1 ELISA Kit 检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥10,800.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：TGFB1, TGB-β1, TGFB
  • 种属：人 (Human); 小鼠 (Mouse)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本

  • 灵敏度：3.36 pg/mL
  • 检测范围：31.25 pg/mL - 2000 pg/mL
  • 回收率：83% - 118%

在售SKU:70-EK981-48, 70-EK981-96

描述

商品名

Human/Mouse TGF-β1 ELISA Kit 检测试剂盒（酶联免疫吸附法）
检测方法

双抗夹心法
精密度

板内变异系数：2.0% - 5.3%；板间变异系数：4.1% - 6.0%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积
  • 人血清：活化样本80 μl + 720 μl 1×检测缓冲液
  • 小鼠血清：活化样本 20 μl + 480 μl 1×检测缓冲液
  • 人/小鼠血浆：活化样本 80 μl + 80μl 1×检测缓冲液
灵敏度

3.36 pg/mL
检测范围

31.25 pg/mL - 2000 pg/mL
回收率

83% - 118%
平均回收率

0.99
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗TGF-β1A单克隆抗体的96孔聚苯乙烯酶标板
  • 人/小鼠TGF-β1A冻干标准品
  • TGF-β1A检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人/小鼠 TGF-β1 抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的 TGF-β1 与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中TGF-β1 的浓度成正比。加入终止液终止反应， 在450nm 波长(参考波长570 - 630 nm)测定吸光度值。

Human/Mouse TGF-β1 ELISA Kit (人/小鼠/大鼠转化生长因子β1 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述

TGFB1 分子靶点信息概述

  • 分子名：TGFB1, transforming growth factor beta 1
  • 基因家族：Transforming growth factor beta family
  • 别名：CED; TGFbeta
  • 曾用名：TGFB; DPD1
  • 全称：Camurati-Engelmann disease; prepro-transforming growth factor beta-1; Diaphyseal dysplasia 1, progressive; transforming growth factor, beta 1

TGFB1 分子靶点综述

转化生长因子β1 (TGF-β1)是转化生长因子β超家族的多肽成员，具有多种细胞学功能，包括调控细胞生长、增殖、分化和凋亡。TGF-β与TGFA协同诱导转化。它也能作为负向自分泌生长因子。TGF-β活化和信号传导的失调可能会引起凋亡。很多细胞可合成TGF-β，且几乎都有特异性的受体。TGF-β1、TGF-β2和TGF-β3都通过相同的受体信号通路发挥功能。TGF-β1对调控免疫系统具有重要作用，对不同类型的细胞或不同发育阶段的细胞具有不同的活性。大部分免疫细胞 (或白细胞) 分泌TGF-β1。TGF-β1与癌症、自身免疫疾病、肝脏疾病、肾脏疾病、糖尿病、心血管疾病、哮喘、慢性阻塞性肺疾病 (COPD)和囊肿性纤维化 (CF) 等相关。

人 Human TGFB1 分子靶点信息

  • 分子名：TGFB1, transforming growth factor beta 1
  • 别称：
    • CED
    • DPD1
    • IBDIMDE
    • LAP
    • latency-associated peptide
    • prepro-transforming growth factor beta-1
    • TGF-beta 1 protein
    • TGF-beta-1
    • TGF-beta1
    • TGFB
    • TGFbeta
    • transforming growth factor beta-1
    • transforming growth factor beta-1 proprotein
    • transforming growth factor beta1
  • 基因序列：NCBI_Gene: 7040
  • 蛋白序列：UniProtKB: P01137

人 Human TGFB1靶点分子功能（预测）

Enables several functions, including antigen binding activity; identical protein binding activity; and transforming growth factor beta receptor binding activity. Involved in several processes, including cell surface receptor signaling pathway; positive regulation of macromolecule metabolic process; and positive regulation of signal transduction. Acts upstream of or within epithelial to mesenchymal transition; negative regulation of gene expression; and positive regulation of isotype switching to IgA isotypes. Located in several cellular components, including cell surface; extracellular space; and nucleus. Colocalizes with microvillus. Implicated in several diseases, including Behcet's disease; Camurati-Engelmann disease; aphthous stomatitis; artery disease (multiple); and lung disease (multiple). Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); carcinoma (multiple); hematologic cancer (multiple); and lung disease (multiple).

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. Bone marrow-derived mesenchymal stem cells attenuate myocardial ischemia–reperfusion injury via upregulation of splenic regulatory T cells 

  2. A pH-responsive polymersome depleting regulatory T cells and blocking A2A receptor for cancer immunotherapy 

  3. Tumor associated neutrophils promote the metastasis of pancreatic ductal adenocarcinoma 

  4. In vivo effect of magnetic microspheres loaded with E2-a in the treatment of alveolar echinococcosis 

  5. Berberine-Loaded Carboxylmethyl Chitosan Nanoparticles Ameliorate DSS-Induced Colitis and Remodel Gut Microbiota in Mice 

  6. Endothelial cells promote triple-negative breast cancer cell metastasis via PAI-1 and CCL5 signaling 

  7. S-allylmercaptocysteine inhibits mucin overexpression and inflammation via MAPKs and PI3K-Akt signaling pathways in acute respiratory distress syndrome 

  8. Enhancing anti-tumor efficacy and immune memory by combining 3p-GPC-3 siRNA treatment with PD-1 blockade in hepatocellular carcinoma 

  9. Involvement of MM cell‑derived exosomes in T lymphocytes immune responses 

  10. Indigo Naturalis Alleviates Dextran Sulfate Sodium-Induced Colitis in Rats via Altering Gut Microbiota 

  11. Tetrahedral Framework Nucleic Acids Inhibit Skin Fibrosis via the Pyroptosis Pathway 

  12. Simultaneous solubilization and extended release of insoluble drug as payload in highly soluble particles of γ-cyclodextrin metal-organic frameworks 

  13. Peritoneal metastatic gastric carcinoma cells exhibit more malignant behavior when co-cultured with HMrSV5 cells 

  14. Tumor-associated macrophages promote pancreatic ductal adenocarcinoma progression by inducing epithelial-to-mesenchymal transition 

  15. Synergistic effect of constituent drugs of Baibutang on improving Yin-deficiency pulmonary fibrosis in rats 

  16. Liuweiwuling tablets attenuate BDL-induced hepatic fibrosis via modulation of TGF-β/Smad and NF-κB signaling pathways 

  17. Combination of Salvia miltiorrhiza and ligustrazine attenuates bleomycin-induced pulmonary fibrosis in rats via modulating TNF-α and TGF-β 

  18. Metabolomics and pharmacodynamic analysis reveal the therapeutic role of Prunella vulgaris oil on intrauterine adhesion rats 

  19. Salt sensitive purely zwitterionic physical hydrogel for prevention of postoperative tissue adhesion 

  20. Analysis of the Potential Relationship between Aging and Pulmonary Fibrosis Based on Transcriptome 

  21. Berberine plays a cardioprotective role by inhibiting macrophage Wnt5a/β-catenin pathway in the myocardium of mice after myocardial infarction 

  22. Protective effects and possible mechanism of 6-gingerol against arsenic trioxide-induced nephrotoxicity based on network pharmacological analysis and experimental validation 

  23. Melatonin regulates the immune response and improves Sjögren's syndrome-like symptoms in NOD/Ltj Mice. 

  24. Cpt1a alleviates cigarette smoke‑induced chronic obstructive pulmonary disease 

  25. Curcumin suppresses the proliferation and tumorigenicity of Cal27 by modulating cancer-associated fibroblasts of TSCC 

  26. Liproxstatin-1 attenuates unilateral ureteral obstruction-induced renal fibrosis by inhibiting renal tubular epithelial cells ferroptosis 

  27. IGFBP7 regulates sepsis-induced epithelial-mesenchymal transition through ERK1/2 signaling 

  28. RORγt agonist synergizes with CTLA-4 antibody to inhibit tumor growth through inhibition of Treg cells via TGF-β signaling in cancer 

  29. Involvement of impaired CD8+ mucosal-associated invariant T cells and myeloid-derived suppressor cells in polycystic ovary syndrome 

  30. BRAFV600E mutant colorectal cancer cells mediate local immunosuppressive microenvironment through exosomal long noncoding RNAs 

  31. Citrus alkaline extracts prevent endoplasmic reticulum stress in type II alveolar epithelial cells to ameliorate pulmonary fibrosis via the ATF3/PINK1 pathway 

  32. Exosomal miR-10b-5p mediates cell communication of gastric cancer cells and fibroblasts and facilitates cell proliferation 

  33. tRNA-Derived Fragment tRF-17-79MP9PP Attenuates Cell Invasion and Migration via THBS1/TGF-β1/Smad3 Axis in Breast Cancer 

  34. High-Dose TGF-β1 Impairs Mesenchymal Stem Cell–Mediated Bone Regeneration via Bmp2 Inhibition 

  35. SGLT2 inhibitors attenuate nephrin loss and enhance TGF-β1 secretion in type 2 diabetes patients with albuminuria: a randomized clinical trial 

  36. Dectin-1 plays a deleterious role in high fat diet-induced NAFLD of mice through enhancing macrophage activation 

  37. Zyxin-Siah2–Lats2 axis mediates cooperation between Hippo and TGF-β signalling pathways 

  38. Novel immunosuppressive effect of FK506 by upregulation of PD-L1 via FKBP51 in heart transplantation 

  39. Iron Overload Impairs Bone Marrow Mesenchymal Stromal Cells from Higher-Risk MDS Patients by Regulating the ROS-Related Wnt/β-Catenin Pathway 

  40. Blood donation improves skin aging through the reduction of iron deposits and the increase of TGF-β1 in elderly skin 

  41. MicroRNA-181b promotes schistosomiasis-induced hepatic fibrosis by targeting Smad7 

  42. Megakaryocytes participate in the occurrence of bleomycin-induced pulmonary fibrosis 

  43. Anti-Fibrosis Effects of Magnesium Lithospermate B in Experimental Pulmonary Fibrosis: By Inhibiting TGF-βRI/Smad Signaling 

  44. PEDF reduces malignant cells proliferation and inhibits the progression of myelofibrosis in myeloproliferative neoplasms 

  45. Roflumilast-Mediated Phosphodiesterase 4D Inhibition Reverses Diabetes-Associated Cardiac Dysfunction and Remodeling: Effects Beyond Glucose Lowering 

  46. Puerarin Ameliorates Caerulein-Induced Chronic Pancreatitis via Inhibition of MAPK Signaling Pathway 

  47. TMEM158 promotes pancreatic cancer aggressiveness by activation of TGFβ1 and PI3K/AKT signaling pathway 

  48. Cardiac microvascular functions improved by MSC-derived exosomes attenuate cardiac fibrosis after ischemia–reperfusion via PDGFR-β modulation 

  49. miR-1273g-3p modulates activation and apoptosis of hepatic stellate cells by directly targeting PTEN in HCV-related liver fibrosis 

  50. YKT6, as a potential predictor of prognosis and immunotherapy response for oral squamous cell carcinoma, is related to cell invasion, metastasis, and CD8+ T cell infiltration 

  51. Hepatoprotective Effect and Potential Mechanism of Aqueous Extract from Phyllanthus emblica on Carbon-Tetrachloride-Induced Liver Fibrosis in Rats 

  52. miR-30c inhibits angiogenesis by targeting delta-like ligand 4 in liver sinusoidal endothelial cell to attenuate liver fibrosis 

  53. PGE2 protects against heart failure through inhibiting TGF-β1 synthesis in cardiomyocytes and crosstalk between TGF-β1 and GRK2 

  54. Platelets promote tumour metastasis via interaction between TLR4 and tumour cell-released high-mobility group box1 protein 

  55. Forecasting sensitive targets of the kynurenine pathway in pancreatic adenocarcinoma using mathematical modeling 

  56. Schisandra Inhibit Bleomycin-Induced Idiopathic Pulmonary Fibrosis in Rats via Suppressing M2 Macrophage Polarization 

  57. Inhibition of miR-182-5p attenuates pulmonary fibrosis via TGF-β/Smad pathway 

  58. Target acquisition of anti-aging manno-oligosaccharide that triggers ECM process via TGF-β/Smads-SIRT1 signalling pathway 

  59. Mesenchymal Stem Cells Alleviate Renal Fibrosis and Inhibit Autophagy via Exosome Transfer of miRNA-122a 

  60. Mechanical Stretching Can Modify the Papillary Dermis Pattern and Papillary Fibroblast Characteristics during Skin Regeneration 

  61. Dasatinib ameliorates chronic pancreatitis induced by caerulein via anti-fibrotic and anti-inflammatory mechanism 

  62. Protective effect of amygdalin on epithelial–mesenchymal transformation in experimental chronic obstructive pulmonary disease mice 

  63. CpG-C ODN M362 as an immunoadjuvant for HBV therapeutic vaccine reverses the systemic tolerance against HBV 

  64. Immobilized Transforming Growth Factor-Beta 1 in a Stiffness-Tunable Artificial Extracellular Matrix Enhances Mechanotransduction in the Epithelial Mesenchymal Transition of Hepatocellular Carcinoma 

  65. The Combination of piR-823 and Eukaryotic Initiation Factor 3 B (EIF3B) Activates Hepatic Stellate Cells via Upregulating TGF-β1 in Liver Fibrogenesis 

  66. Extracellular HMGB1 promotes CD44 expression in hepatocellular carcinoma via regulating miR-21 

  67. Enhanced Expression of ARK5 in Hepatic Stellate Cell and Hepatocyte Synergistically Promote Liver Fibrosis 

  68. Role of the Hippo signaling pathway in safflower yellow pigment treatment of paraquat-induced pulmonary fibrosis 

  69. Systemic osteoprotective effects of Epimedii Folium and Ligustri Lucidi Fructus in senile osteoporosis rats by promoting the osteoblastogenesis and osteoclastogenesis based on MLP-ANN model 

  70. Intensive statin treatment ameliorate the Th17/Treg functional imbalance in patients with non-ST elevation acute coronary syndrome underwent percutaneous coronary intervention 

  71. IL-37 protects against airway remodeling by reversing bronchial epithelial–mesenchymal transition via IL-24 signaling pathway in chronic asthma 

  72. Preventive effects of phenylethanol glycosides from Cistanche tubulosa on bovine serum albumin-induced hepatic fibrosis in rats 

  73. Microarray Profiling of TGF-β1-Induced Long Non-Coding RNA Expression Patterns in Human Lung Bronchial Epithelial BEAS-2B Cells 

  74. Hyperbaric Oxygen Improves the Survival and Angiogenesis of Fat Grafts after Autologous Fat Transplantation 

  75. ROS-responsive liposomes as an inhaled drug delivery nanoplatform for idiopathic pulmonary fibrosis treatment via Nrf2 signaling 

  76. Chondroprotective effects of platelet lysate towards monoiodoacetate-induced arthritis by suppression of TNF-α-induced activation of NF-ĸB pathway in chondrocytes 

  77. Chitosan-Gelatin Scaffolds Incorporating Decellularized Platelet-Rich Fibrin Promote Bone Regeneration 

  78. Nanofat functionalized injectable super-lubricating microfluidic microspheres for treatment of osteoarthritis 

  79. Bortezomib alleviates antibody-mediated rejection in kidney transplantation by facilitating Atg5 expression 

  80. Probiotics ameliorates pulmonary inflammation via modulating gut microbiota and rectifying Th17/Treg imbalance in a rat model of PM2.5 induced lung injury 

  81. miR-181b-5p inhibits endothelial-mesenchymal transition in monocrotaline-induced pulmonary arterial hypertension by targeting endocan and TGFBR1 

  82. Silica dust exposure induces pulmonary fibrosis through autophagy signaling 

  83. Plantaginis Semen polysaccharides ameliorate renal damage through regulating NLRP3 inflammasome in gouty nephropathy rats 

  84. Human amnion-derived mesenchymal stem cells alleviate lung injury induced by white smoke inhalation in rats 

  85. Bone marrow mesenchymal stem cells modified with heme oxygenase-1 alleviate rejection of donation after circulatory death liver transplantation by inhibiting dendritic cell maturation in rats 

  86. Gallic Acid Attenuates Postoperative Intra-Abdominal Adhesion by Inhibiting Inflammatory Reaction in a Rat Model 

  87. Biomimetic micelles to accurately regulate the inflammatory microenvironment for glomerulonephritis treatment 

  88. Louqin Zhisou Decoction Inhibits Mucus Hypersecretion for Acute Exacerbation of Chronic Obstructive Pulmonary Disease Rats by Suppressing EGFR-PI3K-AKT Signaling Pathway and Restoring Th17/Treg Balance 

  89. Glucagon like peptide 2 has a positive impact on osteoporosis in ovariectomized rats 

  90. Changes and significance of inflammatory cytokines in a rat model of cervical spondylosis 

  91. Aerobic Exercise Improves Pulmonary Fibrosis by Improving Insulin Resistance and Inflammation in Obese Mice 

  92. A network pharmacology strategy combined with in vitro experiments to investigate the potential anti-inflammatory mechanism of Prunus cerasifera Ehrhart 

  93. The Nocardia Rubra Cell Wall Skeleton Regulates Macrophages and Promotes Wound Healing 

  94. Exosome-liposome hybrid nanoparticle codelivery of TP and miR497 conspicuously overcomes chemoresistant ovarian cancer 

  95. A programmed surface on polyetheretherketone for sequentially dictating osteoimmunomodulation and bone regeneration to achieve ameliorative osseointegration under osteoporotic conditions 

  96. S-allylmercapto-N-acetylcysteine ameliorates pulmonary fibrosis in mice via Nrf2 pathway activation and NF-κB, TGF-β1/Smad2/3 pathway suppression 

  97. Dual Inhibition of Endoplasmic Reticulum Stress and Oxidation Stress Manipulates the Polarization of Macrophages under Hypoxia to Sensitize Immunotherapy 

  98. Bushen Jianpi Quyu Formula Alleviates Myelosuppression of an Immune-Mediated Aplastic Anemia Mouse Model via Inhibiting Expression of the PI3K/AKT/NF-κB Signaling Pathway 

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  108. Tetrahedral Framework Nucleic Acid Promotes the Treatment of Bisphosphonate-Related Osteonecrosis of the Jaws by Promoting Angiogenesis and M2 Polarization 

  109. Overexpression of Tumor Necrosis Factor-Like Ligand 1 A in Myeloid Cells Aggravates Liver Fibrosis in Mice 

  110. Emodin attenuates silica-induced lung injury by inhibition of inflammation, apoptosis and epithelial-mesenchymal transition 

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  120. Synergistic effects of low‑dose chemotherapy and T cells in renal cell carcinoma 

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