EK1M09

Human MMP-9 ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：MMP9, MMP-9, CLG4B
  • 种属：人 (Human)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：100 μL
  • 灵敏度：1.70 pg/mL
  • 检测范围：0.16 ng/mL - 10 ng/mL
  • 回收率：96% - 108%

在售SKU:70-EK1M09-48, 70-EK1M09-96

描述

商品名

Human MMP-9 ELISA Kit (人基质金属蛋白酶9ELISA 试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：3.9% - 5.7%；板间变异系数：5.8% - 7.8%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

100 μL
灵敏度

1.70 pg/mL
检测范围

0.16 ng/mL - 10 ng/mL
回收率

96% - 108%
平均回收率

1.07
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗MMP-9单克隆抗体的96孔聚苯乙烯酶标板
  • 人MMP-9冻干标准品
  • MMP-9 检测抗体
  • 标准品稀释液
  • 辣根过氧化物酶标记的链霉亲和素
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人MMP-9抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的MMP-9与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中MMP-9的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human MMP-9 ELISA Kit (人基质金属蛋白酶9ELISA 试剂盒) - 标准曲线
标准曲线

分子信息

概述人 MMP9, MMP-9, CLG4B 靶点信息

MMP9 分子靶点信息概述

  • 分子名：MMP9, matrix metallopeptidase 9
  • 基因家族：M10 matrix metallopeptidases
  • 曾用名：CLG4B
  • 全称：matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)

MMP9 分子靶点综述

基质金属蛋白酶9(MMP-9)，又名92kDaIV型胶原酶、92kDa明胶酶或者明胶酶B，是一类属于锌-金属蛋白酶家族的酶。MMP-9参与正常生理过程(如胚胎发育、繁殖、血管形成、骨骼发育、伤口愈细胞迁移、学习和记忆)和病理过程(如关节炎、脑出血和肿瘤转移)的细胞外基质降解。MMP-9和弹性蛋白酶似乎是中性粒细胞迁移通过基底膜的调节因子。MMP-9在血管和新生血管形成中起重要作用。它在人类呼吸道上皮愈合过程中被显著上调，并被发现与许多病理过程相关，如癌症、免疫和心血管疾病。在类风湿性关节炎、局灶性脑缺血案例中可发现MMP-9的水平是升高的。它的水平随着特发性房颤的发展而增加。

人 Human MMP9 分子靶点信息

  • 分子名：MMP9, matrix metallopeptidase 9
  • 别称：
    • 92 kDa gelatinase
    • 92 kDa type IV collagenase
    • CLG4B
    • GELB
    • macrophage gelatinase
    • MANDP2
    • matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
    • matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
    • matrix metalloproteinase-9
    • MMP-9
    • type V collagenase
  • 基因序列：NCBI_Gene: 4318
  • 蛋白序列：UniProtKB: P14780

人 Human MMP9靶点分子功能（预测）

Enables identical protein binding activity; metalloendopeptidase activity; and serine-type endopeptidase activity. Involved in several processes, including cellular response to UV-A; negative regulation of apoptotic signaling pathway; and positive regulation of keratinocyte migration. Located in extracellular space. Implicated in several diseases, including Down syndrome; anodontia; artery disease (multiple); autoimmune disease (multiple); and chronic obstructive pulmonary disease (multiple). Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); brain disease (multiple); carcinoma (multiple); and lung disease (multiple).

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. Tumor associated neutrophils promote the metastasis of pancreatic ductal adenocarcinoma 

  2. SREBP1 silencing inhibits the proliferation and motility of human esophageal squamous carcinoma cells via the Wnt/β‑catenin signaling pathway 

  3. MicroRNA‑675‑3p promotes esophageal squamous cell cancer cell migration and invasion 

  4. Odd-skipped related 1 plays a tumor suppressor role in ovarian cancer via promoting follistatin-like protein 1 transcription 

  5. Rab11a promotes the malignant progression of ovarian cancer by inducing autophagy 

  6. Elevated microRNA‑145‑5p increases matrix metalloproteinase‑9 by activating the nuclear factor‑κB pathway in rheumatoid arthritis 

  7. Insulin Exacerbates Inflammation in Fibroblast-Like Synoviocytes 

  8. Effects of β2/aβ2 on oxLDL-induced CD36 activation in THP-1 macrophages 

  9. CD4+/CD8+ mucosa-associated invariant T cells foster the development of endometriosis: a pilot study 

  10. Lin28/microRNA-let-7a promotes metastasis under circumstances of hyperactive Wnt signaling in esophageal squamous cell carcinoma 

  11. Dysfunction of low-density neutrophils in peripheral circulation in patients with sepsis 

  12. Associations of Interleukin 10, Matrix Metallopeptidase 9, and Legumain with Blood Pressure Variability and Neurologic Outcomes in Patients with Ischemic Stroke 

  13. N-acetylgalactosaminyltransferase GALNT6 is a potential therapeutic target of clear cell renal cell carcinoma progression 

  14. Correlation between matrix metalloproteinase-2, matrix metalloproteinase-9, and tissue inhibitor of metalloproteinases-1 and white matter hyperintensities in patients with cerebral small vessel disease based on cranial magnetic resonance 3D imaging 

  15. NR2F1 overexpression alleviates trophoblast cell dysfunction by inhibiting GDF15/MAPK axis in preeclampsia 

  16. Providing biomimetic microenvironment for pulp regeneration via hydrogel-mediated sustained delivery of tissue-specific developmental signals 

  17. New link between RNH1 and E2F1: regulates the development of lung adenocarcinoma 

  18. PELI1 overexpression contributes to pancreatic cancer progression through upregulating ubiquitination-mediated INPP5J degradation 

  19. Lentinan inhibits cell invasion via M2 polarization of tumor-associated macrophages and Wnt/β-catenin signaling in non-small cell lung cancer 

  20. Stomatin promotes neutrophil degranulation and vascular leakage in the early stage of severe burn injury via enhancement of the intracellular binding of neutrophil primary granules to F-actin 

  21. Kallikrein-Related Peptidase 4 Promotes Proliferation, Migration, Invasion, and Pro-Angiogenesis of Endometrial Stromal Cells via Regulation of Brain-Derived Neurotrophic Factor Production in Endometriosis 

  22. Inhibition of LAMP3 mediates the protective effect of vitamin D against hypoxia/reoxygenation in trophoblast cells 

  23. A novel functional peptide, named EQ-9 (ESETRILLQ), identified by virtual screening from regenerative cell secretome and its potential anti-aging and restoration effects in topical applications 

  24. Rab27b promotes endometriosis by enhancing invasiveness of ESCs and promoting angiogenesis 

  25. Oncogenic Role of the NFATC2/NEDD4/FBP1 Axis in Cholangiocarcinoma 

  26. 腹水 血浆

    CD4+/CD8+ mucosa-associated invariant T cells foster the development of endometriosis: a pilot study 

  27. 腹腔上清液

    Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 

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