Human LIF ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:LIF, CDF, DIA
- 种属:人 (Human)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:50 μL
- 灵敏度:0.64 pg/mL
- 检测范围:15.63 pg/mL - 1000 pg/mL
- 回收率:81% - 117%
在售SKU:70-EK1107-48, 70-EK1107-96, 人, 白血病, 抑制, 因子
描述
商品名 |
Human LIF ELISA Kit (人白血病抑制因子 ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:3.6%-3.8%;板间变异系数:2.0% - 4.8% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
50 μL |
灵敏度 |
0.64 pg/mL |
检测范围 |
15.63 pg/mL - 1000 pg/mL |
回收率 |
81% - 117% |
平均回收率 |
0.97 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人LIF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的LIF与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中LIF的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
LIF 分子靶点信息概述
- 分子名:LIF, LIF interleukin 6 family cytokine
- 基因家族:Receptor ligands; Interleukin 6 type cytokine family
- 别名:CDF; DIA; HILDA
- 全称:differentiation inhibitory activity; differentiation-inducing factor; hepatocyte-stimulating factor III; cholinergic differentiation factor; human interleukin in DA cells; leukemia inhibitory factor; LIF, interleukin 6 family cytokine
LIF 分子靶点综述
白血病抑制因子(LIF)是一种白细胞介素6家族细胞因子,通过抑制分化影响细胞生长。当LIF水平下降时,细胞就会分化。活化的T细胞、单核细胞、星形胶质细胞、成骨细胞、角质形成细胞、肥大细胞和成纤维细胞都可表达LIF。LIF因其能诱导髓系白血病细胞的终端分化,阻止白血病细胞的持续增长而得名。LIF的其它特性包括促进生长、促进不同类型的靶细胞分化、影响骨代谢、恶病质、神经发育、胚胎发育和炎症。LIF参与孕期子宫内膜蜕膜化和胚胎植入子宫内膜的过程。女性体内的LIF和其它细胞因子水平下降时,就容易怀孕,但是不明原因复发性流产的风险会增加。研究表明重组人LIF可能有助于改善女性不明原因不孕不育的着床率。另外,LIF通常用来添加到干细胞培养基中来减少自发分化。
人 Human LIF 分子靶点信息
- 分子名:LIF, LIF interleukin 6 family cytokine
- 别称:
- CDF
- cholinergic differentiation factor
- D factor
- DIA
- differentiation inhibitory activity
- differentiation stimulating factor
- differentiation-inducing factor
- differentiation-stimulating factor
- hepatocyte-stimulating factor III
- HILDA
- human interleukin in DA cells
- leukemia inhibitory factor
- LIF, interleukin 6 family cytokine
- melanoma-derived LPL inhibitor
- MLPLI
- 基因序列:NCBI_Gene: 3976
- 蛋白序列:UniProtKB: P15018
人 Human LIF靶点分子功能(预测)
Enables cytokine activity; growth factor activity; and leukemia inhibitory factor receptor binding activity. Involved in several processes, including leukemia inhibitory factor signaling pathway; positive regulation of protein modification process; and regulation of epithelial cell differentiation involved in kidney development. Acts upstream of or within several processes, including positive regulation of MAPK cascade; positive regulation of macrophage differentiation; and positive regulation of protein phosphorylation. Located in cytosol.
操作步骤
文章目录[隐藏]
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引用文献
文章目录[隐藏]
- Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 
- Metabolic Enzyme Triosephosphate Isomerase 1 and Nicotinamide Phosphoribosyltransferase, Two Independent Inflammatory Indicators in Rheumatoid Arthritis: Evidences From Collagen-Induced Arthritis and Clinical Samples 
- Cancer-associated adipocytes promote the invasion and metastasis in breast cancer through LIF/CXCLs positive feedback loop 
- Glycolysis aggravates methotrexate toxicity by fueling RFC1-controlled intestinal absorption in rheumatic rats 
- Potential biomarkers for diagnosis and assessment of disease activity in systemic lupus erythematosus 
- IDH2/R140Q mutation confers cytokine-independent proliferation of TF-1 cells by activating constitutive STAT3/5 phosphorylation 
- Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 
Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 
Metabolic Enzyme Triosephosphate Isomerase 1 and Nicotinamide Phosphoribosyltransferase, Two Independent Inflammatory Indicators in Rheumatoid Arthritis: Evidences From Collagen-Induced Arthritis and Clinical Samples 
Cancer-associated adipocytes promote the invasion and metastasis in breast cancer through LIF/CXCLs positive feedback loop 
Glycolysis aggravates methotrexate toxicity by fueling RFC1-controlled intestinal absorption in rheumatic rats 
Potential biomarkers for diagnosis and assessment of disease activity in systemic lupus erythematosus 
IDH2/R140Q mutation confers cytokine-independent proliferation of TF-1 cells by activating constitutive STAT3/5 phosphorylation 
- 腹腔上清液
Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 
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