Human IL-9 High Sensitivity ELISA Kit检测试剂盒(酶联免疫吸附法)
¥2,000.00 – ¥3,400.00
- 分子靶点:IL9, IL-9, P40, HP40
- 种属:人 (Human)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:血清血浆:20 μL;细胞培养上清:100 μL
- 灵敏度:0.16 pg/mL
- 检测范围:0.63 pg/mL - 40 pg/mL
- 回收率:98% - 116%
在售SKU:70-EK109HS-48, 70-EK109HS-96, 人白细胞介素9, 白细胞介素9, 白细胞介素, 人, 白介素, 9
描述
商品名 |
Human IL-9 High Sensitivity ELISA Kit (人白细胞介素9高敏ELISA 试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:3.8% - 4.3%;板间变异系数:3.9% - 4.8% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
血清血浆:20 μL;细胞培养上清:100 μL |
灵敏度 |
0.16 pg/mL |
检测范围 |
0.63 pg/mL - 40 pg/mL |
回收率 |
98% - 116% |
平均回收率 |
1.02 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-9抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的IL-9与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后,加入信号增强剂孵育,洗涤去除未结合的物质后,再次加入Streptavidin-HRP。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-9的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
IL9 分子靶点信息概述
- 分子名:IL9, interleukin 9
- 基因家族:Interleukins
- 别名:IL-9; HP40; P40
- 全称:p40 T-cell and mast cell growth factor; T-cell growth factor p40; p40 cytokine; homolog of mouse T cell and mast cell growth factor 40
IL9 分子靶点综述
白细胞介素9(IL-9)是一个分子量为14kDa的糖基化蛋白,包含10个参与二硫键连接的半胱氨酸残基。它是由T细胞特别是CD4+辅助性细胞产生的细胞因子,可调节多种造血细胞。该细胞因子刺激细胞增殖、阻止细胞凋亡。它是通过与IL-9受体结合起作用的,可激活不同的信号转导及激活(STAT)蛋白,从而参与多种生物过程。编码该细胞因子的基因已被确定为哮喘的候选基因。小鼠模型的哮喘遗传研究表明,这种细胞因子是支气管高反应发病机制的一个决定性因素。IL-9介导的自分泌环的存在暗示着一些恶性肿瘤如霍奇金病的存在。IL-9由李-斯二氏细胞、霍奇金淋巴瘤细胞和一些大型再生障碍性淋巴瘤细胞表达,而非霍奇金淋巴瘤和外周T细胞淋巴瘤不表达。
人 Human IL9 分子靶点信息
- 分子名:IL9, interleukin 9
- 别称:
- cytokine P40
- homolog of mouse T cell and mast cell growth factor 40
- HP40
- IL-9
- interleukin-9
- P40
- p40 cytokine
- p40 T-cell and mast cell growth factor
- T-cell growth factor p40
- 基因序列:NCBI_Gene: 3578
- 蛋白序列:UniProtKB: P15248
人 Human IL9靶点分子功能(预测)
Predicted to enable cytokine activity and interleukin-9 receptor binding activity. Predicted to be involved in positive regulation of interleukin-5 production. Predicted to act upstream of or within several processes, including B cell activation; regulation of cellular protein metabolic process; and regulation of receptor signaling pathway via JAK-STAT. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Implicated in asthma and respiratory syncytial virus infectious disease. Biomarker of COVID-19; asthma; cystic fibrosis; lung disease (multiple); and rhinitis.
操作步骤
文章目录[隐藏]
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- 真?假?ELISA试剂盒选择要小心 
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引用文献
文章目录[隐藏]
- Anti−IL-12/23 p40 antibody attenuates chronic graft-versus-host disease with lupus nephritis via inhibiting Tfh cell in mice 
- HMGB1-induced ILC2s activate dendritic cells by producing IL-9 in asthmatic mouse model 
- Acupuncture inhibited airway inflammation and group 2 innate lymphoid cells in the lung in an ovalbumin-induced murine asthma model 
- TL1A modulates the severity of colitis by promoting Th9 differentiation and IL-9 secretion 
- Interleukin-9 blockage reduces early hepatic granuloma formation and fibrosis during Schistosoma japonicum infection in mice 
- The Effect of Entecavir Therapy on Immune Status in Chronic Hepatitis B Patients 
- Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 
- Effect of crosstalk between Th17 and Th9 cells on the activation of dermal vascular smooth muscle cells in systemic scleroderma and regulation of tanshinone IIA 
- Expression profile of PU.1 in CD4+T cells from patients with systemic lupus erythematosus 
- Decreased frequency of circulating Th9 cells in patients with chronic hepatitis B infection 
Anti−IL-12/23 p40 antibody attenuates chronic graft-versus-host disease with lupus nephritis via inhibiting Tfh cell in mice 
HMGB1-induced ILC2s activate dendritic cells by producing IL-9 in asthmatic mouse model 
Acupuncture inhibited airway inflammation and group 2 innate lymphoid cells in the lung in an ovalbumin-induced murine asthma model 
TL1A modulates the severity of colitis by promoting Th9 differentiation and IL-9 secretion 
Interleukin-9 blockage reduces early hepatic granuloma formation and fibrosis during Schistosoma japonicum infection in mice 
The Effect of Entecavir Therapy on Immune Status in Chronic Hepatitis B Patients 
Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 
Effect of crosstalk between Th17 and Th9 cells on the activation of dermal vascular smooth muscle cells in systemic scleroderma and regulation of tanshinone IIA 
Expression profile of PU.1 in CD4+T cells from patients with systemic lupus erythematosus 
Decreased frequency of circulating Th9 cells in patients with chronic hepatitis B infection 
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