EK104HS

Human IL-4 High Sensitivity ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥2,000.00 – ¥3,400.00

  • 分子靶点：IL4, IL-4, BSF1, BCGF1
  • 种属：人 (Human)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：20 μL
  • 灵敏度：0.03 pg/mL
  • 检测范围：0.25 pg/mL - 16 pg/mL
  • 回收率：94% - 105%

在售SKU:70-EK104HS-48, 70-EK104HS-96, 人白细胞介素4, 白细胞介素4, 白细胞介素, 人, 白介素, 4

描述

商品名

Human IL-4 High Sensitivity ELISA Kit (人白介素4高敏 ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：4.2% - 4.8%；板间变异系数：3.6% - 8.1%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

20 μL
灵敏度

0.03 pg/mL
检测范围

0.25 pg/mL - 16 pg/mL
回收率

94% - 105%
平均回收率

0.98
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗IL-4单克隆抗体的96孔聚苯乙烯酶标板
  • 人IL-4冻干标准品
  • IL-4检测抗体
  • 标准品稀释液
  • 辣根过氧化物酶标记的链霉亲和素
  • 信号增强剂浓缩液
  • 信号增强剂稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人 IL-4 抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的 IL-4 与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入 Streptavidin-HRP。洗涤后，加入显色底物 TMB，避光显色。颜色反应的深浅与样本中 IL-4 的浓度成正比。加入终止液终止反应，在 450 nm 波长(参考波长 570 - 630 nm)测定吸光度
值。

Human IL-4 High Sensitivity ELISA Kit (人白介素4高敏 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 IL4, IL-4, BSF1, BCGF1 靶点信息

IL4 分子靶点信息概述

  • 分子名：IL4, interleukin 4
  • 基因家族：Interleukins
  • 别名：BSF1; IL-4; BCGF1; BCGF-1; MGC79402
  • 全称：B_cell stimulatory factor 1; lymphocyte stimulatory factor 1; B cell growth factor 1

IL4 分子靶点综述

IL-4是II型辅助T细胞(Th2细胞)分泌的细胞因子。IL-4是一种多效性细胞因子，在调节T、B淋巴细胞和其他类型细胞的增殖、分化、凋亡，促进以Th2细胞为特征的免疫应答过程中发挥重要作用。IL-4多重生物效应依赖于细胞类型和分化状态，研究表明其在介导过敏性疾病、自身免疫性疾病、感染性疾病、肿瘤等疾病的免疫反应中有多重作用，对肿瘤、自身免疫性疾病和感染性疾病等有治疗作用，并且IL-4对疫苗免疫应答具有调节作用。

人 Human IL4 分子靶点信息

  • 分子名：IL4, interleukin 4
  • 别称：
    • B cell growth factor 1
    • B_cell stimulatory factor 1
    • B-cell stimulatory factor 1
    • BCGF-1
    • BCGF1
    • binetrakin
    • BSF-1
    • BSF1
    • IL-4
    • interleukin 4 variant 2
    • interleukin-4
    • lymphocyte stimulatory factor 1
    • MGC79402
    • pitrakinra
  • 基因序列：NCBI_Gene: 3565
  • 蛋白序列：UniProtKB: P05112

人 Human IL4靶点分子功能（预测）

Predicted to enable cytokine activity. Involved in several processes, including negative regulation of cellular response to transforming growth factor beta stimulus; negative regulation of complement-dependent cytotoxicity; and positive regulation of macromolecule metabolic process. Acts upstream of or within positive regulation of cell migration and positive regulation of cell population proliferation. Predicted to be located in external side of plasma membrane. Predicted to be active in extracellular space. Implicated in several diseases, including asthma (multiple); autoimmune disease (multiple); hepatitis B; hepatitis C; and pancreatic cancer (multiple). Biomarker of several diseases, including allergic rhinitis; alopecia universalis; anogenital venereal wart; autoimmune disease (multiple); and liver disease (multiple).

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. IFNA-AS1 regulates CD4+ T cell activation in myasthenia gravis though HLA-DRB1 

  2. Advanced-platelet-rich fibrin extract promotes adipogenic and osteogenic differentiation of human adipose-derived stem cells in a dose-dependent manner in vitro 

  3. Inflammatory Cytokines Changed in Patients With Depression Before and After Repetitive Transcranial Magnetic Stimulation Treatment 

  4. Vitamin D deficiency is associated with thyroid autoimmunity: results from an epidemiological survey in Tianjin, China 

  5. Progesterone-induced blocking factor-mediated Th1/Th2 balance correlates with fetal arrest in women who underwent in vitro fertilization and embryo transfer 

  6. Adoptive immunotherapy using T lymphocytes redirected to glypican-3 for the treatment of lung squamous cell carcinoma 

  7. Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 

  8. MicroRNA 27b-3p Modulates SYK in Pediatric Asthma Induced by Dust Mites 

  9. Cytokine-induced killer cells efficiently kill stem-like cancer cells of nasopharyngeal carcinoma via the NKG2D-ligands recognition 

  10. Treatment of hepatocellular carcinoma with a GPC3-targeted bispecific T cell engager 

  11. Development of GPC3 and EGFR-dual-targeting chimeric antigen receptor-T cells for adoptive T cell therapy 

  12. Differentiation of T-helper cells in distinct phases of atopic dermatitis involves Th1/Th2 and Th17/Treg 

  13. Immunomodulatory effects of platelets on the severity of hand, foot, and mouth disease infected with enterovirus 71 

  14. Imbalance of Th17, Treg, and helper innate lymphoid cell in the peripheral blood of patients with rheumatoid arthritis 

  15. The Combined Use of Cytokine-Induced Killer Cells and Cyclosporine A for the Treatment of Aplastic Anemia in a Mouse Model 

  16. Identification of Robust Biomarkers for Early Predicting Efficacy of Subcutaneous Immunotherapy in Children With House Dust Mite-Induced Allergic Rhinitis by Multiple Cytokine Profiling 

  17. Aberrant expression of inhibitory receptors on B cells in patients with Graves’ disease 

  18. Human bone marrow mesenchymal stem cells-derived exosomes protect against nerve injury via regulating immune microenvironment in neonatal hypoxic-ischemic brain damage model 

  19. Sputum basophils from allergic asthmatic patients do not express IL-7Rα that is essential for TSLP signalling 

  20. Involvement of the Tim-3 Pathway in the Pathogenesis of Pre-Eclampsia 

  21. Regulation of CBL and ESR1 expression by microRNA-22‑3p, 513a-5p and 625-5p may impact the pathogenesis of dust mite-induced pediatric asthma 

  22. ILC2 Cells Promote Th2 Cell Differentiation in AECOPD Through Activated Notch-GATA3 Signaling Pathway 

  23. Interleukin 3-induced GITR promotes the activation of human basophils 

  24. Induction of the apoptosis, degranulation and IL-13 production of human basophils by butyrate and propionate via suppression of histone deacetylation 

  25. Follistatin-Like Protein-1 Upregulates Dendritic Cell-Based Immunity in Patients with Nasopharyngeal Carcinoma 

  26. Effects of Sarcoptes scabiei Translationally Controlled Tumor Protein (TCTP) on Histamine Release and Degranulation of KU812 Cells 

  27. Expression of coinhibitory PD-L1 on CD4+CD25+FOXP3+ regulatory T cells is elevated in patients with acute coronary syndrome 

  28. Recognition and killing of cancer stem-like cell population in hepatocellular carcinoma cells by cytokine-induced killer cells via NKG2d-ligands recognition 

  29. Infant immune response to hepatitis B vaccine after fetal exposure to telbivudine 

  30. Development of T cells carrying two complementary chimeric antigen receptors against glypican-3 and asialoglycoprotein receptor 1 for the treatment of hepatocellular carcinoma 

  31. Ultrasound-Guided Percutaneous Transhepatic Gallbladder Drainage Improves the Prognosis of Patients with Severe Acute Cholecystitis 

  32. A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide. 

  33. Effect of soothing-liver and nourishing-heart acupuncture on early selective serotonin reuptake inhibitor treatment onset for depressive disorder and related indicators of neuroimmunology: a randomized controlled clinical trial 

  34. Demethoxycurcumin mitigates inflammatory responses in lumbar disc herniation via MAPK and NF-κB pathways in vivo and in vitro 

  35. Revealing the regulation of allergic asthma airway epithelial cell inflammation by STEAP4 targeting MIF through machine learning algorithms and single-cell sequencing analysis 

  36. A citrullinated antigenic vaccine in treatment of autoimmune arthritis 

  37. Solobacterium moorei promotes the progression of adenomatous polyps by causing inflammation and disrupting the intestinal barrier 

  38. Keratinocyte-to-macrophage communication exacerbate psoriasiform dermatitis via LRG1-enriched extracellular vesicles 

  39. Characteristics of Serum Autoantibody Repertoire and Immune Subgroup Variation of Tuberculosis-Associated Obstructive Pulmonary Disease 

  40. CLDN1 silencing suppresses the proliferation and migration of airway smooth muscle cells by modulating MMP14 

  41. CAP2 promotes gastric cancer metastasis by mediating the interaction between tumor cells and tumor-associated macrophages 

  42. The Effect of an Extract of Sappanwood, Protosappanin A and Protosappanin B on Osteogenesis in Periodontitis 

  43. Modified Gegen Qinlian decoction ameliorated ulcerative colitis by attenuating inflammation and oxidative stress and enhancing intestinal barrier function in vivo and in vitro 

  44. MicroRNA-143-3p ameliorates collagen-induced arthritis by polarizing naive CD4+T cells into Treg cells 

  45. A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide 

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