EK133HSS
Human IL-33 High Sensitivity Standard (人白细胞介素33高敏标准品)
¥280.00
因产品会迭代升级，具体实验步骤请按纸质版说明书操作
描述
本产品只包含标准品试剂，如需购买试剂盒请点击下图
-
- EK133HS
- 高敏试剂盒
Human IL-33 High Sensitivity ELISA Kit检测试剂盒（酶联免疫吸附法）
- ¥2,000.00 – ¥3,400.00
| 商品名 |
Human IL-33 High Sensitivity Standard (人白细胞介素33高敏标准品) |
|---|---|
| 组分 |
人 IL-33 高敏标准品 |
| 检测方法 |
双抗夹心法 |
| 样本类型 |
血清，血浆，细胞培养上清及其他生物学样本 |
| 板式 |
管 |
| 保存 |
短期4℃，长期-20℃保存 |
| 运输条件 |
4℃蓝冰运输 |
检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-33抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的IL-33与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-33的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
- 一文掌握ELISA实验显色判断、数据分析及标曲拟合
- 血清OR血浆，哪个是ELISA的菜
- ELISA通关必备丨数据篇丨标准曲线不佳
- ELISA通关必备丨操作篇丨常见问题及解决方案
- ELISA通关必备丨操作篇丨溶解与稀释标准品
- ELISA通关必备丨样本篇丨不常见样本
- ELISA通关必备丨样本篇丨常见样本丨细胞
- ELISA通关必备丨如何选择试剂盒
- ELISA通关必备丨基础知识
- 真？假？ELISA试剂盒选择要小心
- ELISA常见类型一 | 双抗夹心法，你要的都在这里！
- ELISA常见类型二 | 竞争法，五分钟搞定！
- 叮！联科向您投递了个ELISA实验操作干货包，请查收~
- 【视频】ELISA实验操作步骤演示视频教程
- ELISA 组织样本的处理—大鼠组织
- 【视频】ELISA实验原理与常见问题分析
- 查看更多ELISA操作相关问题
ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
文章目录[隐藏]
- Imbalance of Th17, Treg, and helper innate lymphoid cell in the peripheral blood of patients with rheumatoid arthritis
- Effects of myeloid and plasmacytoid dendritic cells on ILC2s in patients with allergic rhinitis
- Soluble ST2 (sST2) as potential marker for hepatic cystic echinococcosis activity
- Altered levels of circulating CD8+CXCR5+PD-1+T follicular cytotoxic cells in primary Sjögren’s syndrome
- Predictive Values of Serum IL-33 and sST2 in Endotypes and Postoperative Recurrence of Chronic Rhinosinusitis with Nasal Polyps
- Lactate Transporter SLC16A3 (MCT4) as an Onco-Immunological Biomarker Associating Tumor Microenvironment and Immune Responses in Lung Cancer
- Revealing the regulation of allergic asthma airway epithelial cell inflammation by STEAP4 targeting MIF through machine learning algorithms and single-cell sequencing analysis
- The egg ribonuclease SjCP1412 accelerates liver fibrosis caused by Schistosoma japonicum infection involving damage associated molecular patterns (DAMPs)
- Shen Qi Wan ameliorates nephritis in chronic kidney disease via AQP1 and DEFB1 regulation
- The enrichment of Arg1+ILC2s and ILCregs facilitates the progression of endometriosis: A preliminary study
Imbalance of Th17, Treg, and helper innate lymphoid cell in the peripheral blood of patients with rheumatoid arthritis
Effects of myeloid and plasmacytoid dendritic cells on ILC2s in patients with allergic rhinitis
Soluble ST2 (sST2) as potential marker for hepatic cystic echinococcosis activity
Altered levels of circulating CD8+CXCR5+PD-1+T follicular cytotoxic cells in primary Sjögren’s syndrome
Predictive Values of Serum IL-33 and sST2 in Endotypes and Postoperative Recurrence of Chronic Rhinosinusitis with Nasal Polyps
Lactate Transporter SLC16A3 (MCT4) as an Onco-Immunological Biomarker Associating Tumor Microenvironment and Immune Responses in Lung Cancer
Revealing the regulation of allergic asthma airway epithelial cell inflammation by STEAP4 targeting MIF through machine learning algorithms and single-cell sequencing analysis
The egg ribonuclease SjCP1412 accelerates liver fibrosis caused by Schistosoma japonicum infection involving damage associated molecular patterns (DAMPs)
Shen Qi Wan ameliorates nephritis in chronic kidney disease via AQP1 and DEFB1 regulation
The enrichment of Arg1+ILC2s and ILCregs facilitates the progression of endometriosis: A preliminary study
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