EK133

Human IL-33 ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

分子靶点：待定
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：50 μL
灵敏度：2.17 pg/mL
检测范围：31.25 pg/mL - 2000 pg/mL
回收率：80% - 116%

在售SKU:70-EK133-48, 70-EK133-96

描述

商品名	Human IL-33 ELISA Kit (人白介素33 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：3.8% - 4.5%；板间变异系数：1.5% - 2.9%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	50 μL
灵敏度	2.17 pg/mL
检测范围	31.25 pg/mL - 2000 pg/mL
回收率	80% - 116%
平均回收率	0.93
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗IL-33单克隆抗体的96孔聚苯乙烯酶标板人IL-33冻干标准品 IL-33检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-33抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-33与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-33的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human IL-33 ELISA Kit (人白介素33 ELISA试剂盒) - 标准曲线 — 标准曲线

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引用文献

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Imbalance of Th17, Treg, and helper innate lymphoid cell in the peripheral blood of patients with rheumatoid arthritis
Soluble ST2 (sST2) as potential marker for hepatic cystic echinococcosis activity
IL-33 promotes the progression of nonrheumatic aortic valve stenosis via inducing differential phenotypic transition in valvular interstitial cells
Altered levels of circulating CD8+CXCR5+PD-1+T follicular cytotoxic cells in primary Sjögren’s syndrome
Predictive Values of Serum IL-33 and sST2 in Endotypes and Postoperative Recurrence of Chronic Rhinosinusitis with Nasal Polyps
Type 2 innate lymphoid cells participate in IL‑33‑stimulated Th2‑associated immune response in chronic obstructive pulmonary disease
Lactate Transporter SLC16A3 (MCT4) as an Onco-Immunological Biomarker Associating Tumor Microenvironment and Immune Responses in Lung Cancer
Revealing the regulation of allergic asthma airway epithelial cell inflammation by STEAP4 targeting MIF through machine learning algorithms and single-cell sequencing analysis
The egg ribonuclease SjCP1412 accelerates liver fibrosis caused by Schistosoma japonicum infection involving damage associated molecular patterns (DAMPs)
Shen Qi Wan ameliorates nephritis in chronic kidney disease via AQP1 and DEFB1 regulation
The enrichment of Arg1+ILC2s and ILCregs facilitates the progression of endometriosis: A preliminary study
Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis

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