EK121

Human IL-21 ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：IL21, IL-21
  • 种属：人 (Human)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：血清血浆：50 μL；细胞培养上清：100 μL
  • 灵敏度：11.99 pg/mL
  • 检测范围：62.5 pg/mL - 4000 pg/mL
  • 回收率：87% - 112%

在售SKU:70-EK121-48, 70-EK121-96

描述

商品名

Human IL-21 ELISA Kit (人白介素21 ELISA 试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：4.5% - 5.1%；板间变异系数：7.2% - 7.7%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

血清血浆：50 μL；细胞培养上清：100 μL
灵敏度

11.99 pg/mL
检测范围

62.5 pg/mL - 4000 pg/mL
回收率

87% - 112%
平均回收率

1.02
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗IL-21单克隆抗体的96孔聚苯乙烯酶标板
  • 人IL-21冻干标准品
  • IL-21检测抗体
  • 标准品稀释液
  • 辣根过氧化物酶标记的链霉亲和素
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-21抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-21与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-21的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human IL-21 ELISA Kit (人白介素21 ELISA 试剂盒) - 标准曲线
标准曲线

分子信息

概述人 IL21, IL-21 靶点信息

IL21 分子靶点信息概述

  • 分子名：IL21, interleukin 21
  • 基因家族：Interleukins
  • 别名：Za11; IL-21

IL21 分子靶点综述

白细胞介素21(IL-21)对免疫细胞具有强烈的调节作用，包括可破坏病毒感染或肿瘤细胞的自然杀伤细胞(NK)和细胞毒性T细胞。该细胞因子可诱导靶细胞分裂/增殖。IL-21表达于人活化的CD4+T细胞，但在其他大多数组织中并没有表达。此外，IL-21在辅助性T细胞亚群Th2和Th17细胞以及滤泡性T细胞中表达上调。而且IL-21还表达于调控这些细胞功能的NKT细胞中。IL-21在免疫炎性疾病中具有病理作用。研究表明存在一个正向自分泌环，可放大并稳定IL-21驱使的、T细胞介导的应答。而且，它还具有抗肿瘤活性，对自身免疫进程具有作用。在临床上，IL-21还可作为潜在的抗肿瘤药物。

人 Human IL21 分子靶点信息

  • 分子名：IL21, interleukin 21
  • 别称：
    • CVID11
    • IL-21
    • interleukin-21
    • interleukin-21 isoform
    • Za11
  • 基因序列：NCBI_Gene: 59067
  • 蛋白序列：UniProtKB: Q9HBE4

人 Human IL21靶点分子功能（预测）

Enables interleukin-2 receptor binding activity. Involved in several processes, including positive regulation of interleukin-17 production; positive regulation of natural killer cell mediated cytotoxicity; and tyrosine phosphorylation of STAT protein. Acts upstream of or within positive regulation of B cell proliferation; positive regulation of T cell proliferation; and positive regulation of tyrosine phosphorylation of STAT protein. Predicted to be located in extracellular region. Implicated in asthma; common variable immunodeficiency; hepatocellular carcinoma; and systemic lupus erythematosus. Biomarker of several diseases, including anogenital venereal wart; autoimmune disease (multiple); dengue hemorrhagic fever; human immunodeficiency virus infectious disease; and tropical spastic paraparesis.

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. Vitamin D deficiency is associated with thyroid autoimmunity: results from an epidemiological survey in Tianjin, China 

  2. Interleukin-21 Inhibits HBV Replication in vitro 

  3. Original research: Switch receptor T3/28 improves long-term persistence and antitumor efficacy of CAR-T cells 

  4. Circulating memory T follicular helper subsets, Tfh2 and Tfh17, participate in the pathogenesis of Guillain-Barré syndrome 

  5. Circulating Memory T Follicular Helper Cells in Patients with Neuromyelitis Optica/Neuromyelitis Optica Spectrum Disorders 

  6. Altered levels of circulating CD8+CXCR5+PD-1+T follicular cytotoxic cells in primary Sjögren’s syndrome 

  7. The ratio of circulating follicular T helper cell to follicular T regulatory cell is correlated with disease activity in systemic lupus erythematosus 

  8. Circulating CCR7+ICOS+ Memory T Follicular Helper Cells in Patients with Multiple Sclerosis 

  9. Increased CD4+CXCR5+T follicular helper cells in diabetic nephropathy 

  10. Increased circulating CXCR5+ CD4+ T follicular helper-like cells in oral lichen planus 

  11. Double Photosystems-Based ‘Z-Scheme’ Photoelectrochemical Sensing Mode for Ultrasensitive Detection of Disease Biomarker Accompanying Three-Dimensional DNA Walker 

  12. Targeting the class IA PI3K isoforms p110α/δ attenuates heart allograft rejection in mice by suppressing the CD4+ T lymphocyte response 

  13. Tonsillar Microbiome-Derived Lantibiotics Induce Structural Changes of IL-6 and IL-21 Receptors and Modulate Host Immunity 

  14. Efficacy and safety of low-dose interleukin-2 in combination with methotrexate in patients with active rheumatoid arthritis: a randomized, double-blind, placebo-controlled phase 2 trial 

  15. IgD-activated mast cells induce IgE synthesis in B cells in nasal polyps 

  16. Changes in regulatory B cells and their relationship with rheumatoid arthritis disease activity 

  17. Amelioration of immunoglobulin A vasculitis by suppression of the pathological expansion of T follicular helper 17?cells 

  18. Lyophilized lymph nodes for improved delivery of chimeric antigen receptor T cells 

  19. The regulation of Tfh cell differentiation by β-hydroxybutyrylation modification of transcription factor Bcl6 

  20. Imbalance of follicular regulatory T (Tfr) cells/follicular helper T (Tfh) cells in adult patients with primary immune thrombocytopenia 

  21. Modified Gegen Qinlian decoction ameliorated ulcerative colitis by attenuating inflammation and oxidative stress and enhancing intestinal barrier function in vivo and in vitro 

  22. Circulating Memory T Follicular Helper Subsets, Tfh2 and Tfh17, Participate in the Pathogenesis of Guillain-Barré Syndrome 

  23. Circulating Ccr7+Icos+ Memory T Follicular Helper Cells in Patients with Multiple Sclerosis 

  24. Circulating Memory T Follicular Helper Cells in Patients with Neuromyelitis Optica/Neuromyelitis Optica Spectrum Disorders 

  25. Increased Circulating Cxcr5(+) Cd4(+) T Follicular Helper-Like Cells in Oral Lichen Planus 

  26. The Ratio of Circulating Follicular T Helper Cell to Follicular T Regulatory Cell Is Correlated with Disease Activity in Systemic Lupus Erythematosus 

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