Human IL-18 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:IL18, IGIF, IL1F4
- 种属:人 (Human)
- 样本类型:血清,血浆,细胞培养上清
- 检测样本体积:血清血浆:20 μL;细胞培养上清:100 μL
- 灵敏度:19.52 pg/mL
- 检测范围:0.16 ng/mL - 10 ng/mL
- 回收率:87% - 114%
在售SKU:70-EK118-48, 70-EK118-96
描述
商品名 |
Human IL-18 ELISA Kit (人白介素18 ELISA 试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:3.4% - 6.4%;板间变异系数:3.4% - 4.7% |
样本类型 |
血清,血浆,细胞培养上清 |
检测样本体积 |
血清血浆:20 μL;细胞培养上清:100 μL |
灵敏度 |
19.52 pg/mL |
检测范围 |
0.16 ng/mL - 10 ng/mL |
回收率 |
87% - 114% |
平均回收率 |
1.00 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-18抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和辣根过氧化物酶标记检测抗体,经过孵育,样本中存在的IL-18与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-18的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
IL18 分子靶点信息概述
- 分子名:IL18, interleukin 18
- 基因家族:Interleukins
- 别名:IGIF; IL1F4; IL-1g; IL-18
- 全称:interferon-gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor)
IL18 分子靶点综述
白细胞介素18(IL-18),又名干扰素γ诱导因子,是一种促炎性细胞因子,在人类中由IL18基因编码。IL-18属于IL-1超家族,由巨噬细胞和其它细胞分泌产生。IL-18刺激后,自然杀伤细胞(NK)和某些T细胞就会释放另一种重要的细胞因子—干扰素γ或Ⅱ型干扰素,进而激活巨噬细胞或其它细胞。IL-18可诱导产生严重的炎症反应,同样可增加人类神经元中β-淀粉样蛋白(与阿尔茨海默氏病相关)的产生。
人 Human IL18 分子靶点信息
- 分子名:IL18, interleukin 18
- 别称:
- iboctadekin
- IFN-gamma-inducing factor
- IGIF
- IL-1 gamma
- IL-18
- IL-1g
- IL1F4
- interferon gamma-inducing factor
- interleukin 18 (interferon-gamma-inducing factor)
- interleukin-1 gamma
- interleukin-18
- MGC12320
- 基因序列:NCBI_Gene: 3606
- 蛋白序列:UniProtKB: Q14116
人 Human IL18靶点分子功能(预测)
Enables cytokine activity and interleukin-18 receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of intracellular signal transduction; and positive regulation of macromolecule metabolic process. Located in extracellular space. Implicated in several diseases, including Kawasaki disease; allergic rhinitis; autoimmune disease (multiple); biliary atresia; and hepatitis B. Biomarker of several diseases, including autoimmune disease (multiple); cholestasis (multiple); liver disease (multiple); lung disease (multiple); and myositis (multiple).
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
- 血清OR血浆,哪个是ELISA的菜 
- ELISA通关必备丨数据篇丨标准曲线不佳 
- ELISA通关必备丨操作篇丨常见问题及解决方案 
- ELISA通关必备丨操作篇丨溶解与稀释标准品 
- ELISA通关必备丨样本篇丨不常见样本 
- ELISA通关必备丨样本篇丨常见样本丨细胞 
- ELISA通关必备丨如何选择试剂盒 
- ELISA通关必备丨基础知识 
- 真?假?ELISA试剂盒选择要小心 
- ELISA常见类型一 | 双抗夹心法,你要的都在这里! 
- ELISA常见类型二 | 竞争法,五分钟搞定! 
- 叮!联科向您投递了个ELISA实验操作干货包,请查收~ 
- 【视频】ELISA实验操作步骤演示视频教程 
- ELISA 组织样本的处理—大鼠组织 
- 【视频】ELISA实验原理与常见问题分析 
- 查看更多ELISA操作相关问题
ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
文章目录[隐藏]
- Suppression of NOD-like receptor protein 3 inflammasome activation and macrophage M1 polarization by hederagenin contributes to attenuation of sepsis-induced acute lung injury in rats 
- Involvement of impaired CD8+ mucosal-associated invariant T cells and myeloid-derived suppressor cells in polycystic ovary syndrome 
- Overexpression of TOLLIP Protects against Acute Kidney Injury after Paraquat Intoxication through Inhibiting NLRP3 Inflammasome Activation Modulated by Toll-Like Receptor 2/4 Signaling 
- Smoking increases oral mucosa susceptibility to Candida albicans infection via the Nrf2 pathway: In vitro and animal studies 
- miR-146a is directly regulated by STAT3 in human hepatocellular carcinoma cells and involved in anti-tumor immune suppression 
- Dimethyl fumarate ameliorates lipopolysaccharide-induced acute lung injury by inhibiting NLRP3 inflammasome-mediated pyroptosis through enhancing Nrf2 signaling 
- Role of ASM/Cer/TXNIP signaling module in the NLRP3 inflammasome activation 
- USP22 suppresses the NLRP3 inflammasome by degrading NLRP3 via ATG5-dependent autophagy 
- Identification of Tubocapsanolide A as a novel NLRP3 inhibitor for potential treatment of colitis 
- GSDMD contributes to myocardial reperfusion injury by regulating pyroptosis 
- Fibroblast growth factor 10 protects against particulate matter-induced lung injury by inhibiting oxidative stress-mediated pyroptosis via the PI3K/Akt/Nrf2 signaling pathway 
- The combined action of monocytic myeloid-derived suppressor cells and mucosal-associated invariant T cells promotes the progression of cervical cancer 
- Piperlongumine induces apoptosis and reduces bortezomib resistance by inhibiting STAT3 in multiple myeloma cells 
- Licochalcone E improves insulin sensitivity in palmitic acid-treated HepG2 cells through inhibition of the NLRP3 signaling pathway 
- MiR-146a-5p Mimic Inhibits NLRP3 Inflammasome Downstream Inflammatory Factors and CLIC4 in Neonatal Necrotizing Enterocolitis 
- Dynamic changes of innate lymphoid cells in acute ST-segment elevation myocardial infarction and its association with clinical outcomes 
- CD4+/CD8+ mucosa-associated invariant T cells foster the development of endometriosis: a pilot study 
- Mdivi-1 alleviates atopic dermatitis through the inhibition of NLRP3 inflammasome 
- A novel function of NLRP3 independent of inflammasome as a key transcription factor of IL-33 in epithelial cells of atopic dermatitis 
- Astragaloside IV attenuate MI-induced myocardial fibrosis and cardiac remodeling by inhibiting ROS/caspase-1/GSDMD signaling pathway 
- Hypaphorine exerts anti-inflammatory effects in sepsis induced acute lung injury via modulating DUSP1/p38/JNK pathway 
- α-NETA induces pyroptosis of epithelial ovarian cancer cells through the GSDMD/caspase-4 pathway 
- Comparison of morphology, phenotypes and function between cultured human IL‑4‑DC and IFN‑DC 
- Participation of Increased Circulating MAIT Cells in Lung Cancer: a Pilot Study 
- SARS-CoV-2 N protein promotes NLRP3 inflammasome activation to induce hyperinflammation 
- RIPK3-Dependent Necroptosis Is Induced and Restricts Viral Replication in Human Astrocytes Infected With Zika Virus 
- NLRP3 inflammasome-mediated pyroptosis contributes to the pathogenesis of non-ischemic dilated cardiomyopathy 
- MiR-21-5p Induces Pyroptosis in Colorectal Cancer via TGFBI 
- Citric acid of ovarian cancer metabolite induces pyroptosis via the caspase-4/TXNIP-NLRP3-GSDMD pathway in ovarian cancer 
- Hypothermia Inhibits Cerebral Necroptosis and NOD-Like Receptor Pyrin Domain Containing 3 Pathway in a Swine Model of Cardiac Arrest 
- Monocytes Undergo Functional Reprogramming to Generate Immunosuppression through HIF-1α Signaling Pathway in the Late Phase of Sepsis 
- NLRP3 inflammasome and lipid metabolism analysis based on UPLC-Q-TOF-MS in gouty nephropathy 
- Pyroptosis engagement and bladder urothelial cell-derived exosomes recruit mast cells and induce barrier dysfunction of bladder urothelium after uropathogenic E. coli infection 
- Association between Nod‑like receptor protein 3 inflammasome and gouty nephropathy 
- Serum microtubule-associated protein light chain 3 type II levels correlate with aggravation and multi-organ dysfunction in septic patients 
- Neutrophil Extracellular Traps Augmented Alveolar Macrophage Pyroptosis via AIM2 Inflammasome Activation in LPS-Induced ALI/ARDS 
- miR-513c-5p Suppression Aggravates Pyroptosis of Endothelial Cell in Deep Venous Thrombosis by Promoting Caspase-1 
- Levels of Interleukin-1β, Interleukin-18, and Tumor Necrosis Factor-α in Cerebrospinal Fluid of Aneurysmal Subarachnoid Hemorrhage Patients May Be Predictors of Early Brain Injury and Clinical Prognosis 
- Overexpression of TRIM3 protects against LPS-induced acute kidney injury via repressing IRF3 pathway and NLRP3 inflammasome 
- The role of α5-nicotinic acetylcholine receptor/NLRP3 signaling pathway in lung adenocarcinoma cell proliferation and migration 
- TIM3/CEACAM1 pathway involves in myeloid-derived suppressor cells induced CD8+ T cells exhaustion and bone marrow inflammatory microenvironment in myelodysplastic syndrome 
- NLRP3 inflammasome is required for apoptosis of Aggregatibacter actinomycetemcomitans-infected human osteoblastic MG63 cells 
- miR-155 promotes macrophage pyroptosis induced by Porphyromonas gingivalis through regulating the NLRP3 inflammasome 
- The anti-periodontitis action of metformin via targeting NLRP3 inflammasome 
- Pericentriolar material 1 promotes intestinal inflammation in ulcerative colitis by activating NLRP3/gasdermin D-mediated macrophage pyroptosis 
- Pectolinarigenin targeting FGFR3 alleviates osteoarthritis progression by regulating the NF-κB/NLRP3 inflammasome pyroptotic pathway 
- Hydrogen sulfide mitigates ox?LDL?induced NLRP3/caspase?1/GSDMD dependent macrophage pyroptosis by S?sulfhydrating caspase?1 
- Novel Isoalantolactone-Based Derivatives as Potent NLRP3 Inflammasome Inhibitors: Design, Synthesis, and Biological Characterization 
- Fabrication of a Coculture Organoid Model in the Biomimetic Matrix of Alginate to Investigate Breast Cancer Progression in a TAMs-Leading Immune Microenvironment 
- FABP4 in macrophages facilitates obesity-associated pancreatic cancer progression via the NLRP3/IL-1β axis 
- A study on the level of NLRP3 inflammasome in patients with premature rupture of membranes 
- Mesenchymal stem cells overexpressing PBX1 alleviates haemorrhagic shock-induced kidney damage by inhibiting NF-κB activation 
- SMAD2 inhibits pyroptosis of fibroblast-like synoviocytes and secretion of inflammatory factors via the TGF-β pathway in rheumatoid arthritis 
- Huoxue Jiedu Recipe represses mitochondrial fission to alleviate submandibular gland inflammation in Sj?gren's syndrome 
- LMCD1 is involved in tubulointerstitial inflammation in the early phase of renal fibrosis by promoting NFATc1-mediated NLRP3 activation 
- Coptisine mitigates diabetic nephropathy via repressing the NRLP3 inflammasome 
- Circ_0001818 targets miR-136-5p to increase lipopolysaccharide-induced HK2 cell injuries by activating TXNIP/NLRP3 inflammasome pathway 
- Ginsenoside Rh3 induces pyroptosis and ferroptosis through the Stat3/p53/NRF2 axis in colorectal cancer cells 
- Hyperoside exerts protective effects against anticardiolipin antibody-induced recurrent pregnancy loss in vivo and in vitro 
- Sleep Deprivation Promotes Endothelial Inflammation and Atherogenesis by Reducing Exosomal miR-182-5p 
- Tilianin Protects against Nonalcoholic Fatty Liver Disease in Early Obesity Mice 
- Knocking down GALNT6 promotes pyroptosis of pancreatic ductal adenocarcinoma cells through NF-κB/NLRP3/GSDMD and GSDME signaling pathway. 
- The sodium new houttuyfonate suppresses NSCLC via activating pyroptosis through TCONS-14036/miR-1228-5p/PRKCDBP pathway 
- USP22 suppresses the NLRP3 inflammasome by degrading NLRP3 via ATG5-dependent autophagy 
- CD4+/CD8+ mucosa-associated invariant T cells foster the development of endometriosis: a pilot study 
- Participation of Increased Circulating MAIT Cells in Lung Cancer: a Pilot Study 
- Sars-Cov-2 N Protein Promotes Nlrp3 Inflammasome Activation to Induce Hyperinflammation 
- Involvement of Impaired Cd8+ Mucosal-Associated Invariant T Cells and Myeloid-Derived Suppressor Cells in Polycystic Ovary Syndrome 
- Citric acid of ovarian cancer metabolite induces pyroptosis via the caspase-4/TXNIP-NLRP3-GSDMD pathway in ovarian cancer 
- A novel function of NLRP3 independent of inflammasome as a key transcription factor of IL-33 in epithelial cells of atopic dermatitis 
- Α-Neta Induces Pyroptosis of Epithelial Ovarian Cancer Cells through the Gsdmd/Caspase-4 Pathway 
- Lymphotoxins Promote the Progression of Human Lymphatic Malformation by Enhancing Lymphatic Endothelial Cell Proliferation 
Suppression of NOD-like receptor protein 3 inflammasome activation and macrophage M1 polarization by hederagenin contributes to attenuation of sepsis-induced acute lung injury in rats 
Involvement of impaired CD8+ mucosal-associated invariant T cells and myeloid-derived suppressor cells in polycystic ovary syndrome 
Overexpression of TOLLIP Protects against Acute Kidney Injury after Paraquat Intoxication through Inhibiting NLRP3 Inflammasome Activation Modulated by Toll-Like Receptor 2/4 Signaling 
Smoking increases oral mucosa susceptibility to Candida albicans infection via the Nrf2 pathway: In vitro and animal studies 
miR-146a is directly regulated by STAT3 in human hepatocellular carcinoma cells and involved in anti-tumor immune suppression 
Dimethyl fumarate ameliorates lipopolysaccharide-induced acute lung injury by inhibiting NLRP3 inflammasome-mediated pyroptosis through enhancing Nrf2 signaling 
Role of ASM/Cer/TXNIP signaling module in the NLRP3 inflammasome activation 
USP22 suppresses the NLRP3 inflammasome by degrading NLRP3 via ATG5-dependent autophagy 
Identification of Tubocapsanolide A as a novel NLRP3 inhibitor for potential treatment of colitis 
GSDMD contributes to myocardial reperfusion injury by regulating pyroptosis 
Fibroblast growth factor 10 protects against particulate matter-induced lung injury by inhibiting oxidative stress-mediated pyroptosis via the PI3K/Akt/Nrf2 signaling pathway 
The combined action of monocytic myeloid-derived suppressor cells and mucosal-associated invariant T cells promotes the progression of cervical cancer 
Piperlongumine induces apoptosis and reduces bortezomib resistance by inhibiting STAT3 in multiple myeloma cells 
Licochalcone E improves insulin sensitivity in palmitic acid-treated HepG2 cells through inhibition of the NLRP3 signaling pathway 
MiR-146a-5p Mimic Inhibits NLRP3 Inflammasome Downstream Inflammatory Factors and CLIC4 in Neonatal Necrotizing Enterocolitis 
Dynamic changes of innate lymphoid cells in acute ST-segment elevation myocardial infarction and its association with clinical outcomes 
CD4+/CD8+ mucosa-associated invariant T cells foster the development of endometriosis: a pilot study 
Mdivi-1 alleviates atopic dermatitis through the inhibition of NLRP3 inflammasome 
A novel function of NLRP3 independent of inflammasome as a key transcription factor of IL-33 in epithelial cells of atopic dermatitis 
Astragaloside IV attenuate MI-induced myocardial fibrosis and cardiac remodeling by inhibiting ROS/caspase-1/GSDMD signaling pathway 
Hypaphorine exerts anti-inflammatory effects in sepsis induced acute lung injury via modulating DUSP1/p38/JNK pathway 
α-NETA induces pyroptosis of epithelial ovarian cancer cells through the GSDMD/caspase-4 pathway 
Comparison of morphology, phenotypes and function between cultured human IL‑4‑DC and IFN‑DC 
Participation of Increased Circulating MAIT Cells in Lung Cancer: a Pilot Study 
SARS-CoV-2 N protein promotes NLRP3 inflammasome activation to induce hyperinflammation 
RIPK3-Dependent Necroptosis Is Induced and Restricts Viral Replication in Human Astrocytes Infected With Zika Virus 
NLRP3 inflammasome-mediated pyroptosis contributes to the pathogenesis of non-ischemic dilated cardiomyopathy 
MiR-21-5p Induces Pyroptosis in Colorectal Cancer via TGFBI 
Citric acid of ovarian cancer metabolite induces pyroptosis via the caspase-4/TXNIP-NLRP3-GSDMD pathway in ovarian cancer 
Hypothermia Inhibits Cerebral Necroptosis and NOD-Like Receptor Pyrin Domain Containing 3 Pathway in a Swine Model of Cardiac Arrest 
Monocytes Undergo Functional Reprogramming to Generate Immunosuppression through HIF-1α Signaling Pathway in the Late Phase of Sepsis 
NLRP3 inflammasome and lipid metabolism analysis based on UPLC-Q-TOF-MS in gouty nephropathy 
Pyroptosis engagement and bladder urothelial cell-derived exosomes recruit mast cells and induce barrier dysfunction of bladder urothelium after uropathogenic E. coli infection 
Association between Nod‑like receptor protein 3 inflammasome and gouty nephropathy 
Serum microtubule-associated protein light chain 3 type II levels correlate with aggravation and multi-organ dysfunction in septic patients 
Neutrophil Extracellular Traps Augmented Alveolar Macrophage Pyroptosis via AIM2 Inflammasome Activation in LPS-Induced ALI/ARDS 
miR-513c-5p Suppression Aggravates Pyroptosis of Endothelial Cell in Deep Venous Thrombosis by Promoting Caspase-1 
Levels of Interleukin-1β, Interleukin-18, and Tumor Necrosis Factor-α in Cerebrospinal Fluid of Aneurysmal Subarachnoid Hemorrhage Patients May Be Predictors of Early Brain Injury and Clinical Prognosis 
Overexpression of TRIM3 protects against LPS-induced acute kidney injury via repressing IRF3 pathway and NLRP3 inflammasome 
The role of α5-nicotinic acetylcholine receptor/NLRP3 signaling pathway in lung adenocarcinoma cell proliferation and migration 
TIM3/CEACAM1 pathway involves in myeloid-derived suppressor cells induced CD8+ T cells exhaustion and bone marrow inflammatory microenvironment in myelodysplastic syndrome 
NLRP3 inflammasome is required for apoptosis of Aggregatibacter actinomycetemcomitans-infected human osteoblastic MG63 cells 
miR-155 promotes macrophage pyroptosis induced by Porphyromonas gingivalis through regulating the NLRP3 inflammasome 
The anti-periodontitis action of metformin via targeting NLRP3 inflammasome 
Pericentriolar material 1 promotes intestinal inflammation in ulcerative colitis by activating NLRP3/gasdermin D-mediated macrophage pyroptosis 
Pectolinarigenin targeting FGFR3 alleviates osteoarthritis progression by regulating the NF-κB/NLRP3 inflammasome pyroptotic pathway 
Hydrogen sulfide mitigates ox?LDL?induced NLRP3/caspase?1/GSDMD dependent macrophage pyroptosis by S?sulfhydrating caspase?1 
Novel Isoalantolactone-Based Derivatives as Potent NLRP3 Inflammasome Inhibitors: Design, Synthesis, and Biological Characterization 
Fabrication of a Coculture Organoid Model in the Biomimetic Matrix of Alginate to Investigate Breast Cancer Progression in a TAMs-Leading Immune Microenvironment 
FABP4 in macrophages facilitates obesity-associated pancreatic cancer progression via the NLRP3/IL-1β axis 
A study on the level of NLRP3 inflammasome in patients with premature rupture of membranes 
Mesenchymal stem cells overexpressing PBX1 alleviates haemorrhagic shock-induced kidney damage by inhibiting NF-κB activation 
SMAD2 inhibits pyroptosis of fibroblast-like synoviocytes and secretion of inflammatory factors via the TGF-β pathway in rheumatoid arthritis 
Huoxue Jiedu Recipe represses mitochondrial fission to alleviate submandibular gland inflammation in Sj?gren's syndrome 
LMCD1 is involved in tubulointerstitial inflammation in the early phase of renal fibrosis by promoting NFATc1-mediated NLRP3 activation 
Coptisine mitigates diabetic nephropathy via repressing the NRLP3 inflammasome 
Circ_0001818 targets miR-136-5p to increase lipopolysaccharide-induced HK2 cell injuries by activating TXNIP/NLRP3 inflammasome pathway 
Ginsenoside Rh3 induces pyroptosis and ferroptosis through the Stat3/p53/NRF2 axis in colorectal cancer cells 
Hyperoside exerts protective effects against anticardiolipin antibody-induced recurrent pregnancy loss in vivo and in vitro 
Sleep Deprivation Promotes Endothelial Inflammation and Atherogenesis by Reducing Exosomal miR-182-5p 
Tilianin Protects against Nonalcoholic Fatty Liver Disease in Early Obesity Mice 
Knocking down GALNT6 promotes pyroptosis of pancreatic ductal adenocarcinoma cells through NF-κB/NLRP3/GSDMD and GSDME signaling pathway. 
The sodium new houttuyfonate suppresses NSCLC via activating pyroptosis through TCONS-14036/miR-1228-5p/PRKCDBP pathway 
- 小鼠腹膜巨噬细胞 细胞培养上清 血清
USP22 suppresses the NLRP3 inflammasome by degrading NLRP3 via ATG5-dependent autophagy 
- 腹水 血浆
CD4+/CD8+ mucosa-associated invariant T cells foster the development of endometriosis: a pilot study 
- 血清
Participation of Increased Circulating MAIT Cells in Lung Cancer: a Pilot Study 
- 细胞培养上清 血清
Sars-Cov-2 N Protein Promotes Nlrp3 Inflammasome Activation to Induce Hyperinflammation 
- 血浆
Involvement of Impaired Cd8+ Mucosal-Associated Invariant T Cells and Myeloid-Derived Suppressor Cells in Polycystic Ovary Syndrome 
- 细胞培养上清
Citric acid of ovarian cancer metabolite induces pyroptosis via the caspase-4/TXNIP-NLRP3-GSDMD pathway in ovarian cancer 
- 组织匀浆 细胞培养上清 耳朵(匀浆) 血清
A novel function of NLRP3 independent of inflammasome as a key transcription factor of IL-33 in epithelial cells of atopic dermatitis 
Α-Neta Induces Pyroptosis of Epithelial Ovarian Cancer Cells through the Gsdmd/Caspase-4 Pathway 
Lymphotoxins Promote the Progression of Human Lymphatic Malformation by Enhancing Lymphatic Endothelial Cell Proliferation 
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