EK118

Human IL-18 ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：IL18, IGIF, IL1F4
  • 种属：人 (Human)
  • 样本类型：血清，血浆，细胞培养上清
  • 检测样本体积：血清血浆：20 μL；细胞培养上清：100 μL
  • 灵敏度：19.52 pg/mL
  • 检测范围：0.16 ng/mL - 10 ng/mL
  • 回收率：87% - 114%

在售SKU:70-EK118-48, 70-EK118-96

描述

商品名

Human IL-18 ELISA Kit (人白介素18 ELISA 试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：3.4% - 6.4%；板间变异系数：3.4% - 4.7%
样本类型

血清，血浆，细胞培养上清
检测样本体积

血清血浆：20 μL；细胞培养上清：100 μL
灵敏度

19.52 pg/mL
检测范围

0.16 ng/mL - 10 ng/mL
回收率

87% - 114%
平均回收率

1.00
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗IL-18单克隆抗体的96孔聚苯乙烯酶标板
  • 人IL-18冻干标准品
  • 检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 显色底物(TMB)
  • 终止液
  • 洗液(20×)

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-18抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和辣根过氧化物酶标记检测抗体，经过孵育，样本中存在的IL-18与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-18的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630 nm)测定吸光度值。

Human IL-18 ELISA Kit (人白介素18 ELISA 试剂盒) - 标准曲线
标准曲线

分子信息

概述人 IL18, IGIF, IL1F4 靶点信息

IL18 分子靶点信息概述

  • 分子名：IL18, interleukin 18
  • 基因家族：Interleukins
  • 别名：IGIF; IL1F4; IL-1g; IL-18
  • 全称：interferon-gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor)

IL18 分子靶点综述

白细胞介素18(IL-18)，又名干扰素γ诱导因子，是一种促炎性细胞因子，在人类中由IL18基因编码。IL-18属于IL-1超家族，由巨噬细胞和其它细胞分泌产生。IL-18刺激后，自然杀伤细胞(NK)和某些T细胞就会释放另一种重要的细胞因子—干扰素γ或Ⅱ型干扰素，进而激活巨噬细胞或其它细胞。IL-18可诱导产生严重的炎症反应，同样可增加人类神经元中β-淀粉样蛋白(与阿尔茨海默氏病相关)的产生。

人 Human IL18 分子靶点信息

  • 分子名：IL18, interleukin 18
  • 别称：
    • iboctadekin
    • IFN-gamma-inducing factor
    • IGIF
    • IL-1 gamma
    • IL-18
    • IL-1g
    • IL1F4
    • interferon gamma-inducing factor
    • interleukin 18 (interferon-gamma-inducing factor)
    • interleukin-1 gamma
    • interleukin-18
    • MGC12320
  • 基因序列：NCBI_Gene: 3606
  • 蛋白序列：UniProtKB: Q14116

人 Human IL18靶点分子功能（预测）

Enables cytokine activity and interleukin-18 receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of intracellular signal transduction; and positive regulation of macromolecule metabolic process. Located in extracellular space. Implicated in several diseases, including Kawasaki disease; allergic rhinitis; autoimmune disease (multiple); biliary atresia; and hepatitis B. Biomarker of several diseases, including autoimmune disease (multiple); cholestasis (multiple); liver disease (multiple); lung disease (multiple); and myositis (multiple).

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]

  1. Suppression of NOD-like receptor protein 3 inflammasome activation and macrophage M1 polarization by hederagenin contributes to attenuation of sepsis-induced acute lung injury in rats 

  2. Involvement of impaired CD8+ mucosal-associated invariant T cells and myeloid-derived suppressor cells in polycystic ovary syndrome 

  3. Overexpression of TOLLIP Protects against Acute Kidney Injury after Paraquat Intoxication through Inhibiting NLRP3 Inflammasome Activation Modulated by Toll-Like Receptor 2/4 Signaling 

  4. Smoking increases oral mucosa susceptibility to Candida albicans infection via the Nrf2 pathway: In vitro and animal studies 

  5. miR-146a is directly regulated by STAT3 in human hepatocellular carcinoma cells and involved in anti-tumor immune suppression 

  6. Dimethyl fumarate ameliorates lipopolysaccharide-induced acute lung injury by inhibiting NLRP3 inflammasome-mediated pyroptosis through enhancing Nrf2 signaling 

  7. Role of ASM/Cer/TXNIP signaling module in the NLRP3 inflammasome activation 

  8. USP22 suppresses the NLRP3 inflammasome by degrading NLRP3 via ATG5-dependent autophagy 

  9. Identification of Tubocapsanolide A as a novel NLRP3 inhibitor for potential treatment of colitis 

  10. GSDMD contributes to myocardial reperfusion injury by regulating pyroptosis 

  11. Fibroblast growth factor 10 protects against particulate matter-induced lung injury by inhibiting oxidative stress-mediated pyroptosis via the PI3K/Akt/Nrf2 signaling pathway 

  12. The combined action of monocytic myeloid-derived suppressor cells and mucosal-associated invariant T cells promotes the progression of cervical cancer 

  13. Piperlongumine induces apoptosis and reduces bortezomib resistance by inhibiting STAT3 in multiple myeloma cells 

  14. Licochalcone E improves insulin sensitivity in palmitic acid-treated HepG2 cells through inhibition of the NLRP3 signaling pathway 

  15. MiR-146a-5p Mimic Inhibits NLRP3 Inflammasome Downstream Inflammatory Factors and CLIC4 in Neonatal Necrotizing Enterocolitis 

  16. Dynamic changes of innate lymphoid cells in acute ST-segment elevation myocardial infarction and its association with clinical outcomes 

  17. CD4+/CD8+ mucosa-associated invariant T cells foster the development of endometriosis: a pilot study 

  18. Mdivi-1 alleviates atopic dermatitis through the inhibition of NLRP3 inflammasome 

  19. A novel function of NLRP3 independent of inflammasome as a key transcription factor of IL-33 in epithelial cells of atopic dermatitis 

  20. Astragaloside IV attenuate MI-induced myocardial fibrosis and cardiac remodeling by inhibiting ROS/caspase-1/GSDMD signaling pathway 

  21. Hypaphorine exerts anti-inflammatory effects in sepsis induced acute lung injury via modulating DUSP1/p38/JNK pathway 

  22. α-NETA induces pyroptosis of epithelial ovarian cancer cells through the GSDMD/caspase-4 pathway 

  23. Comparison of morphology, phenotypes and function between cultured human IL‑4‑DC and IFN‑DC 

  24. Participation of Increased Circulating MAIT Cells in Lung Cancer: a Pilot Study 

  25. SARS-CoV-2 N protein promotes NLRP3 inflammasome activation to induce hyperinflammation 

  26. RIPK3-Dependent Necroptosis Is Induced and Restricts Viral Replication in Human Astrocytes Infected With Zika Virus 

  27. NLRP3 inflammasome-mediated pyroptosis contributes to the pathogenesis of non-ischemic dilated cardiomyopathy 

  28. MiR-21-5p Induces Pyroptosis in Colorectal Cancer via TGFBI 

  29. Citric acid of ovarian cancer metabolite induces pyroptosis via the caspase-4/TXNIP-NLRP3-GSDMD pathway in ovarian cancer 

  30. Hypothermia Inhibits Cerebral Necroptosis and NOD-Like Receptor Pyrin Domain Containing 3 Pathway in a Swine Model of Cardiac Arrest 

  31. Monocytes Undergo Functional Reprogramming to Generate Immunosuppression through HIF-1α Signaling Pathway in the Late Phase of Sepsis 

  32. NLRP3 inflammasome and lipid metabolism analysis based on UPLC-Q-TOF-MS in gouty nephropathy 

  33. Pyroptosis engagement and bladder urothelial cell-derived exosomes recruit mast cells and induce barrier dysfunction of bladder urothelium after uropathogenic E. coli infection 

  34. Association between Nod‑like receptor protein 3 inflammasome and gouty nephropathy 

  35. Serum microtubule-associated protein light chain 3 type II levels correlate with aggravation and multi-organ dysfunction in septic patients 

  36. Neutrophil Extracellular Traps Augmented Alveolar Macrophage Pyroptosis via AIM2 Inflammasome Activation in LPS-Induced ALI/ARDS 

  37. miR-513c-5p Suppression Aggravates Pyroptosis of Endothelial Cell in Deep Venous Thrombosis by Promoting Caspase-1 

  38. Levels of Interleukin-1β, Interleukin-18, and Tumor Necrosis Factor-α in Cerebrospinal Fluid of Aneurysmal Subarachnoid Hemorrhage Patients May Be Predictors of Early Brain Injury and Clinical Prognosis 

  39. Overexpression of TRIM3 protects against LPS-induced acute kidney injury via repressing IRF3 pathway and NLRP3 inflammasome 

  40. The role of α5-nicotinic acetylcholine receptor/NLRP3 signaling pathway in lung adenocarcinoma cell proliferation and migration 

  41. TIM3/CEACAM1 pathway involves in myeloid-derived suppressor cells induced CD8+ T cells exhaustion and bone marrow inflammatory microenvironment in myelodysplastic syndrome 

  42. NLRP3 inflammasome is required for apoptosis of Aggregatibacter actinomycetemcomitans-infected human osteoblastic MG63 cells 

  43. miR-155 promotes macrophage pyroptosis induced by Porphyromonas gingivalis through regulating the NLRP3 inflammasome 

  44. The anti-periodontitis action of metformin via targeting NLRP3 inflammasome 

  45. Pericentriolar material 1 promotes intestinal inflammation in ulcerative colitis by activating NLRP3/gasdermin D-mediated macrophage pyroptosis 

  46. Pectolinarigenin targeting FGFR3 alleviates osteoarthritis progression by regulating the NF-κB/NLRP3 inflammasome pyroptotic pathway 

  47. Hydrogen sulfide mitigates ox?LDL?induced NLRP3/caspase?1/GSDMD dependent macrophage pyroptosis by S?sulfhydrating caspase?1 

  48. Novel Isoalantolactone-Based Derivatives as Potent NLRP3 Inflammasome Inhibitors: Design, Synthesis, and Biological Characterization 

  49. Fabrication of a Coculture Organoid Model in the Biomimetic Matrix of Alginate to Investigate Breast Cancer Progression in a TAMs-Leading Immune Microenvironment 

  50. FABP4 in macrophages facilitates obesity-associated pancreatic cancer progression via the NLRP3/IL-1β axis 

  51. A study on the level of NLRP3 inflammasome in patients with premature rupture of membranes 

  52. Mesenchymal stem cells overexpressing PBX1 alleviates haemorrhagic shock-induced kidney damage by inhibiting NF-κB activation 

  53. SMAD2 inhibits pyroptosis of fibroblast-like synoviocytes and secretion of inflammatory factors via the TGF-β pathway in rheumatoid arthritis 

  54. Huoxue Jiedu Recipe represses mitochondrial fission to alleviate submandibular gland inflammation in Sj?gren's syndrome 

  55. LMCD1 is involved in tubulointerstitial inflammation in the early phase of renal fibrosis by promoting NFATc1-mediated NLRP3 activation 

  56. Coptisine mitigates diabetic nephropathy via repressing the NRLP3 inflammasome 

  57. Circ_0001818 targets miR-136-5p to increase lipopolysaccharide-induced HK2 cell injuries by activating TXNIP/NLRP3 inflammasome pathway 

  58. Ginsenoside Rh3 induces pyroptosis and ferroptosis through the Stat3/p53/NRF2 axis in colorectal cancer cells 

  59. Hyperoside exerts protective effects against anticardiolipin antibody-induced recurrent pregnancy loss in vivo and in vitro 

  60. Sleep Deprivation Promotes Endothelial Inflammation and Atherogenesis by Reducing Exosomal miR-182-5p 

  61. Tilianin Protects against Nonalcoholic Fatty Liver Disease in Early Obesity Mice 

  62. Knocking down GALNT6 promotes pyroptosis of pancreatic ductal adenocarcinoma cells through NF-κB/NLRP3/GSDMD and GSDME signaling pathway. 

  63. The sodium new houttuyfonate suppresses NSCLC via activating pyroptosis through TCONS-14036/miR-1228-5p/PRKCDBP pathway 

  64. 小鼠腹膜巨噬细胞 细胞培养上清 血清

    USP22 suppresses the NLRP3 inflammasome by degrading NLRP3 via ATG5-dependent autophagy 

  65. 腹水 血浆

    CD4+/CD8+ mucosa-associated invariant T cells foster the development of endometriosis: a pilot study 

  66. 血清

    Participation of Increased Circulating MAIT Cells in Lung Cancer: a Pilot Study 

  67. 细胞培养上清 血清

    Sars-Cov-2 N Protein Promotes Nlrp3 Inflammasome Activation to Induce Hyperinflammation 

  68. 血浆

    Involvement of Impaired Cd8+ Mucosal-Associated Invariant T Cells and Myeloid-Derived Suppressor Cells in Polycystic Ovary Syndrome 

  69. 细胞培养上清

    Citric acid of ovarian cancer metabolite induces pyroptosis via the caspase-4/TXNIP-NLRP3-GSDMD pathway in ovarian cancer 

  70. 组织匀浆 细胞培养上清 耳朵（匀浆） 血清

    A novel function of NLRP3 independent of inflammasome as a key transcription factor of IL-33 in epithelial cells of atopic dermatitis 

  71. Α-Neta Induces Pyroptosis of Epithelial Ovarian Cancer Cells through the Gsdmd/Caspase-4 Pathway 

  72. Lymphotoxins Promote the Progression of Human Lymphatic Malformation by Enhancing Lymphatic Endothelial Cell Proliferation 

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