EK117HS

Human IL-17A High Sensitivity ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥2,000.00 – ¥3,400.00

  • 分子靶点：IL17A, IL17, CTLA8
  • 种属：人 (Human)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：血清血浆：20 μL；细胞培养上清：100 μL
  • 灵敏度：0.20 pg/mL
  • 检测范围：0.78 pg/mL - 50 pg/mL
  • 回收率：80% - 95%

在售SKU:70-EK117HS-48, 70-EK117HS-96

描述

商品名

Human IL-17A High Sensitivity ELISA Kit (人白介素17A 高敏ELISA 试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：4.3% - 4.6%；板间变异系数：5.9% - 9.0%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

血清血浆：20 μL；细胞培养上清：100 μL
灵敏度

0.20 pg/mL
检测范围

0.78 pg/mL - 50 pg/mL
回收率

80% - 95%
平均回收率

0.86
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗IL-17A单克隆抗体的96孔聚苯乙烯酶标板
  • 人IL-17A冻干标准品
  • IL-17A检测抗体
  • 标准品稀释液
  • 辣根过氧化物酶标记的链霉亲和素
  • 信号增强剂浓缩液
  • 信号增强剂稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-17A抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-17A与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-17A的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human IL-17A High Sensitivity ELISA Kit (人白介素17A 高敏ELISA 试剂盒) - 标准曲线
标准曲线

分子信息

概述人 IL17A, IL17, CTLA8 靶点信息

IL17A 分子靶点信息概述

  • 分子名：IL17A, interleukin 17A
  • 基因家族：Interleukins
  • 别名：IL-17A; IL-17
  • 曾用名：CTLA8; IL17
  • 全称：cytotoxic T-lymphocyte-associated protein 8; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)

IL17A 分子靶点综述

IL-17主要由Th17型细胞产生，是目前已发现的30余种白细胞介素中的一员，按序号排在第17位。该家族包括6个成员配体(IL-17A ~ IL-17F)和5个受体(IL-17RA ~ IL-17RD和SEF)。IL-17是一种主要由活化的T细胞产生的致炎细胞因子，可以促进T细胞的激活和刺激上皮细胞、内皮细胞、成纤维细胞产生多种细胞因子，从而导致炎症的产生。

人 Human IL17A 分子靶点信息

  • 分子名：IL17A, interleukin 17A
  • 别称：
    • CTLA-8
    • CTLA8
    • cytotoxic T-lymphocyte-associated antigen 8
    • cytotoxic T-lymphocyte-associated protein 8
    • cytotoxic T-lymphocyte-associated serine esterase 8
    • IL-17
    • IL-17A
    • IL17
    • ILA17
    • interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
    • interleukin-17
    • interleukin-17A
  • 基因序列：NCBI_Gene: 3605
  • 蛋白序列：UniProtKB: Q16552

人 Human IL17A靶点分子功能（预测）

Predicted to enable cytokine activity; protein heterodimerization activity; and protein homodimerization activity. Involved in several processes, including interleukin-17-mediated signaling pathway; positive regulation of cytokine production; and positive regulation of osteoclast differentiation. Acts upstream of or within positive regulation of cytokine production and positive regulation of transcription by RNA polymerase II. Predicted to be located in extracellular region. Implicated in Mycobacterium avium complex disease; autoimmune disease (multiple); bronchial disease (multiple); macular degeneration; and rhinitis (multiple). Biomarker of several diseases, including acne; autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and rhinitis (multiple).

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. RORγt agonist synergizes with CTLA-4 antibody to inhibit tumor growth through inhibition of Treg cells via TGF-β signaling in cancer 

  2. Involvement of impaired CD8+ mucosal-associated invariant T cells and myeloid-derived suppressor cells in polycystic ovary syndrome 

  3. RAS-association domain family 1A regulates the abnormal cell proliferation in psoriasis via inhibition of Yes-associated protein 

  4. Vitamin D deficiency is associated with thyroid autoimmunity: results from an epidemiological survey in Tianjin, China 

  5. Elevated Peripheral Frequencies of Th22 Cells: A Novel Potent Participant in Obesity and Type 2 Diabetes 

  6. CD4+/CD8+ mucosa-associated invariant T cells foster the development of endometriosis: a pilot study 

  7. Vitamin D Deficiency Contributes to the Reduction and Impaired Function of Naïve Regulatory T Cell in Chronic Heart Failure 

  8. Differentiation of T-helper cells in distinct phases of atopic dermatitis involves Th1/Th2 and Th17/Treg 

  9. Immunomodulatory effects of platelets on the severity of hand, foot, and mouth disease infected with enterovirus 71 

  10. Imbalance of Th17, Treg, and helper innate lymphoid cell in the peripheral blood of patients with rheumatoid arthritis 

  11. Metabolic Enzyme Triosephosphate Isomerase 1 and Nicotinamide Phosphoribosyltransferase, Two Independent Inflammatory Indicators in Rheumatoid Arthritis: Evidences From Collagen-Induced Arthritis and Clinical Samples 

  12. Triptolide inhibits Th17 differentiation via controlling PKM2-mediated glycolysis in rheumatoid arthritis 

  13. MicroRNA-26b-5p alleviates murine collagen-induced arthritis by modulating Th17 cell plasticity 

  14. Total extract of Xin Jia Xuan Bai Cheng Qi decoction inhibits pulmonary fibrosis via the TGF-β/Smad signaling pathways in vivo and in vitro 

  15. Low Ratio of Treg to Th17 Cells After 36 Weeks of Telbivudine Therapy Predict HBeAg Seroconversion 

  16. Basal level of Th17 immune response is not enhanced in aplastic amemia 

  17. Involvement of interleukin-21 in the pathophysiology of aplastic anemia 

  18. Targeting the class IA PI3K isoforms p110α/δ attenuates heart allograft rejection in mice by suppressing the CD4+ T lymphocyte response 

  19. Glycolysis aggravates methotrexate toxicity by fueling RFC1-controlled intestinal absorption in rheumatic rats 

  20. Tonsillar Microbiome-Derived Lantibiotics Induce Structural Changes of IL-6 and IL-21 Receptors and Modulate Host Immunity 

  21. Changes of Th17/Tc17 and Th17/Treg cells in endometrial carcinoma 

  22. Fibrinogen-Like Protein 1 as a Novel Biomarker of Psoriasis Severity 

  23. Berberine inhibits dendritic cells differentiation in DSS-induced colitis by promoting Bacteroides fragilis 

  24. Participation of Increased Circulating MAIT Cells in Lung Cancer: a Pilot Study 

  25. Distribution of Th17 cells and Foxp3-expressing T cells in tumor-infiltrating lymphocytes in patients with uterine cervical cancer 

  26. Efficacy and safety of low-dose interleukin-2 in combination with methotrexate in patients with active rheumatoid arthritis: a randomized, double-blind, placebo-controlled phase 2 trial 

  27. A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide. 

  28. Interleukin-25 is upregulated in patients with systemic lupus erythematosus and ameliorates murine lupus by inhibiting inflammatory cytokine production 

  29. Colorectal Cancer Progression Is Associated with Accumulation of Th17 Lymphocytes in Tumor Tissues and Increased Serum Levels of Interleukin-6 

  30. Amelioration of immunoglobulin A vasculitis by suppression of the pathological expansion of T follicular helper 17?cells 

  31. A citrullinated antigenic vaccine in treatment of autoimmune arthritis 

  32. Gut Microbiota Disorder Contributes to the Production of IL-17A That Exerts Chemotaxis via Binding to IL-17RA in Endometriosis 

  33. Effects of silencing NLRP3 gene on proliferation of psoriasis-like HaCaT cells and expressions of cytokines 

  34. Small extracellular vesicles derived from human mesenchymal stem cells prevent Th17-dominant neutrophilic airway inflammation via immunoregulation on Th17 cells 

  35. DPSCs regulate epithelial-T cell interactions in oral submucous fibrosis 

  36. Periodontitis aggravates COPD through the activation of γδ T cell and M2 macrophage 

  37. Characteristics of Serum Autoantibody Repertoire and Immune Subgroup Variation of Tuberculosis-Associated Obstructive Pulmonary Disease 

  38. Fe-curcumin nanozyme-mediated immunosuppression and anti-inflammation in experimental autoimmune uveitis 

  39. SLAMF3 promotes Th17 differentiation and is reversed by iguratimod through JAK1/STAT3 pathway in primary Sj?gren's syndrome 

  40. BMI-specific inflammatory response to phthalate exposure in early pregnancy: findings from the TMCHESC study 

  41. A role of NR4A2 in Graves’ disease: regulation of Th17/Treg 

  42. Hyperoside alleviates postmenopausal osteoporosis via regulating miR-19a-5p/IL-17A axis 

  43. Modified Gegen Qinlian decoction ameliorated ulcerative colitis by attenuating inflammation and oxidative stress and enhancing intestinal barrier function in vivo and in vitro 

  44. MicroRNA-143-3p ameliorates collagen-induced arthritis by polarizing naive CD4+T cells into Treg cells 

  45. Human colon carcinogenesis is associated with increased interleukin-17-driven inflammatory responses 

  46. A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide 

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