EK117

Human IL-17A ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：IL17A, IL17, CTLA8
  • 种属：人 (Human)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：50 μL
  • 灵敏度：0.55 pg/mL
  • 检测范围：7.83 pg/mL - 500 pg/mL
  • 回收率：94% - 113%

在售SKU:70-EK117/2-48, 70-EK117/2-96

描述

商品名

Human IL-17A ELISA Kit (人白介素17A ELISA 试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：3.5% - 4.3%；板间变异系数：2.8% - 3.3%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

50 μL
灵敏度

0.55 pg/mL
检测范围

7.83 pg/mL - 500 pg/mL
回收率

94% - 113%
平均回收率

1.02
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗IL-17A单克隆抗体的96孔聚苯乙烯酶标板
  • 人IL-17A冻干标准品
  • IL-17A检测抗体
  • 标准品稀释液
  • 辣根过氧化物酶标记的链霉亲和素
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-17A抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-17A与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-17A的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human IL-17A ELISA Kit (人白介素17A ELISA 试剂盒) - 标准曲线
标准曲线

分子信息

概述人 IL17A, IL17, CTLA8 靶点信息

IL17A 分子靶点信息概述

  • 分子名：IL17A, interleukin 17A
  • 基因家族：Interleukins
  • 别名：IL-17A; IL-17
  • 曾用名：CTLA8; IL17
  • 全称：cytotoxic T-lymphocyte-associated protein 8; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)

IL17A 分子靶点综述

IL-17主要由Th17型细胞产生，是目前已发现的30余种白细胞介素中的一员，按序号排在第17位。该家族包括6个成员配体(IL-17A ~ IL-17F)和5个受体(IL-17RA ~ IL-17RD和SEF)。IL-17是一种主要由活化的T细胞产生的致炎细胞因子，可以促进T细胞的激活和刺激上皮细胞、内皮细胞、成纤维细胞产生多种细胞因子，从而导致炎症的产生。

人 Human IL17A 分子靶点信息

  • 分子名：IL17A, interleukin 17A
  • 别称：
    • CTLA-8
    • CTLA8
    • cytotoxic T-lymphocyte-associated antigen 8
    • cytotoxic T-lymphocyte-associated protein 8
    • cytotoxic T-lymphocyte-associated serine esterase 8
    • IL-17
    • IL-17A
    • IL17
    • ILA17
    • interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
    • interleukin-17
    • interleukin-17A
  • 基因序列：NCBI_Gene: 3605
  • 蛋白序列：UniProtKB: Q16552

人 Human IL17A靶点分子功能（预测）

Predicted to enable cytokine activity; protein heterodimerization activity; and protein homodimerization activity. Involved in several processes, including interleukin-17-mediated signaling pathway; positive regulation of cytokine production; and positive regulation of osteoclast differentiation. Acts upstream of or within positive regulation of cytokine production and positive regulation of transcription by RNA polymerase II. Predicted to be located in extracellular region. Implicated in Mycobacterium avium complex disease; autoimmune disease (multiple); bronchial disease (multiple); macular degeneration; and rhinitis (multiple). Biomarker of several diseases, including acne; autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and rhinitis (multiple).

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

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引用文献

文章目录[隐藏]

  1. RORγt inhibitor SR1001 alleviates acute pancreatitis by suppressing pancreatic IL-17-producing Th17 and γδ-T cells in mice with ceruletide-induced pancreatitis 

  2. RORγt agonist synergizes with CTLA-4 antibody to inhibit tumor growth through inhibition of Treg cells via TGF-β signaling in cancer 

  3. Involvement of impaired CD8+ mucosal-associated invariant T cells and myeloid-derived suppressor cells in polycystic ovary syndrome 

  4. RAS-association domain family 1A regulates the abnormal cell proliferation in psoriasis via inhibition of Yes-associated protein 

  5. Vitamin D deficiency is associated with thyroid autoimmunity: results from an epidemiological survey in Tianjin, China 

  6. Elevated Peripheral Frequencies of Th22 Cells: A Novel Potent Participant in Obesity and Type 2 Diabetes 

  7. CD4+/CD8+ mucosa-associated invariant T cells foster the development of endometriosis: a pilot study 

  8. Vitamin D Deficiency Contributes to the Reduction and Impaired Function of Naïve Regulatory T Cell in Chronic Heart Failure 

  9. Differentiation of T-helper cells in distinct phases of atopic dermatitis involves Th1/Th2 and Th17/Treg 

  10. Immunomodulatory effects of platelets on the severity of hand, foot, and mouth disease infected with enterovirus 71 

  11. Imbalance of Th17, Treg, and helper innate lymphoid cell in the peripheral blood of patients with rheumatoid arthritis 

  12. Increased AHR Transcripts Correlate With Pro-inflammatory T-Helper Lymphocytes Polarization in Both Metabolically Healthy Obesity and Type 2 Diabetic Patients 

  13. Metabolic Enzyme Triosephosphate Isomerase 1 and Nicotinamide Phosphoribosyltransferase, Two Independent Inflammatory Indicators in Rheumatoid Arthritis: Evidences From Collagen-Induced Arthritis and Clinical Samples 

  14. Total extract of Xin Jia Xuan Bai Cheng Qi decoction inhibits pulmonary fibrosis via the TGF-β/Smad signaling pathways in vivo and in vitro 

  15. Low Ratio of Treg to Th17 Cells After 36 Weeks of Telbivudine Therapy Predict HBeAg Seroconversion 

  16. Basal level of Th17 immune response is not enhanced in aplastic amemia 

  17. Involvement of interleukin-21 in the pathophysiology of aplastic anemia 

  18. Interleukin‑17 activates JAK2/STAT3, PI3K/Akt and nuclear factor‑κB signaling pathway to promote the tumorigenesis of cervical cancer 

  19. Interleukin-35 inhibits angiogenesis through T helper17/ Interleukin-17 related signaling pathways in IL-1β-stimulated SW1353 cells 

  20. The STING antagonist H-151 ameliorates psoriasis via suppression of STING/NF-κB-mediated inflammation 

  21. Metabolomics Profiles Associated with the Treatment of Zuojin Pill on Patients with Chronic Nonatrophic Gastritis 

  22. Glycolysis aggravates methotrexate toxicity by fueling RFC1-controlled intestinal absorption in rheumatic rats 

  23. Wnt/β-catenin signaling mediates the abnormal osteogenic and adipogenic capabilities of bone marrow mesenchymal stem cells from chronic graft-versus-host disease patients 

  24. Tonsillar Microbiome-Derived Lantibiotics Induce Structural Changes of IL-6 and IL-21 Receptors and Modulate Host Immunity 

  25. Changes of Th17/Tc17 and Th17/Treg cells in endometrial carcinoma 

  26. Berberine inhibits dendritic cells differentiation in DSS-induced colitis by promoting Bacteroides fragilis 

  27. Distribution of Th17 cells and Foxp3-expressing T cells in tumor-infiltrating lymphocytes in patients with uterine cervical cancer 

  28. A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide. 

  29. Colorectal Cancer Progression Is Associated with Accumulation of Th17 Lymphocytes in Tumor Tissues and Increased Serum Levels of Interleukin-6 

  30. Amelioration of immunoglobulin A vasculitis by suppression of the pathological expansion of T follicular helper 17?cells 

  31. A citrullinated antigenic vaccine in treatment of autoimmune arthritis 

  32. Gut Microbiota Disorder Contributes to the Production of IL-17A That Exerts Chemotaxis via Binding to IL-17RA in Endometriosis 

  33. IFN-α affects Th17/Treg cell balance through c-Maf and associated with the progression of EBV- SLE 

  34. Effects of silencing NLRP3 gene on proliferation of psoriasis-like HaCaT cells and expressions of cytokines 

  35. Small extracellular vesicles derived from human mesenchymal stem cells prevent Th17-dominant neutrophilic airway inflammation via immunoregulation on Th17 cells 

  36. DPSCs regulate epithelial-T cell interactions in oral submucous fibrosis 

  37. Characteristics of Serum Autoantibody Repertoire and Immune Subgroup Variation of Tuberculosis-Associated Obstructive Pulmonary Disease 

  38. Fe-curcumin nanozyme-mediated immunosuppression and anti-inflammation in experimental autoimmune uveitis 

  39. BMI-specific inflammatory response to phthalate exposure in early pregnancy: findings from the TMCHESC study 

  40. A role of NR4A2 in Graves’ disease: regulation of Th17/Treg 

  41. Hyperoside alleviates postmenopausal osteoporosis via regulating miR-19a-5p/IL-17A axis 

  42. Modified Gegen Qinlian decoction ameliorated ulcerative colitis by attenuating inflammation and oxidative stress and enhancing intestinal barrier function in vivo and in vitro 

  43. Human colon carcinogenesis is associated with increased interleukin-17-driven inflammatory responses 

  44. A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide 

  45. 腹水 血浆

    CD4+/CD8+ mucosa-associated invariant T cells foster the development of endometriosis: a pilot study 

  46. 腹腔上清液

    Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 

  47. 血清

    Participation of Increased Circulating MAIT Cells in Lung Cancer: a Pilot Study 

  48. 血浆

    Involvement of Impaired Cd8+ Mucosal-Associated Invariant T Cells and Myeloid-Derived Suppressor Cells in Polycystic Ovary Syndrome 

  49. 细胞培养上清 血清

    Casein Kinase II exacerbates rheumatoid arthritis via promoting Th1 and Th17 cell inflammatory responses 

  50. Low Ratio of Treg to Th17 Cells after 36 Weeks of Telbivudine Therapy Predict Hbeag Seroconversion 

  51. Human Colon Carcinogenesis Is Associated with Increased Interleukin-17-Driven Inflammatory Responses 

  52. Basal Level of Th17 Immune Response Is Not Enhanced in Aplastic Amemia 

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