Human IL-15 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:IL15
- 种属:人 (Human)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:50 μL
- 灵敏度:3.78 pg/mL
- 检测范围:3.13 pg/mL - 200 pg/mL
- 回收率:87% - 111%
在售SKU:70-EK115-48, 70-EK115-96
描述
商品名 |
Human IL-15 ELISA Kit (人白细胞介素15ELISA 试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:7.1% - 9.0%;板间变异系数:7.8% - 9.5% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
50 μL |
灵敏度 |
3.78 pg/mL |
检测范围 |
3.13 pg/mL - 200 pg/mL |
回收率 |
87% - 111% |
平均回收率 |
0.97 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-15抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-15与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-15的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
IL15 分子靶点信息概述
- 分子名:IL15, interleukin 15
- 基因家族:Interleukins
- 别名:IL-15; MGC9721
IL15 分子靶点综述
白细胞介素15(IL-15)是一种促炎性蛋白,在中性粒细胞、树突状细胞和巨噬细胞的活化中起作用,对于NK细胞和CD8+T细胞的发育和生存是必不可少的。IL-15通过异三聚体受体信号激活Jak/Stat途径。虽然IL-15mRNA在许多细胞类型中表达,但是该分泌蛋白在生物样品中很少检测得到。最近的研究表明,IL-15滞留在胞内,只有当与其唯一的受体IL-15Rα形成复合物才能分泌到胞外。可溶性IL-15/IL-15R复合物由小鼠树突状细胞、成纤维细胞和巨噬细胞产生,它的结合活性比单独的IL-15提高10-100倍。
人 Human IL15 分子靶点信息
- 分子名:IL15, interleukin 15
- 别称:
- IL-15
- interleukin-15
- MGC9721
- 基因序列:NCBI_Gene: 3600
- 蛋白序列:UniProtKB: P40933
人 Human IL15靶点分子功能(预测)
Predicted to enable cytokine activity. Involved in several processes, including cell surface receptor signaling pathway; macrophage differentiation; and positive regulation of macromolecule metabolic process. Located in cytosol and nuclear speck. Implicated in aspergillosis; asthma; pleurisy; and type 1 diabetes mellitus. Biomarker of several diseases, including artery disease (multiple); brain infarction; lung disease (multiple); obesity; and viral infectious disease (multiple).
操作步骤
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引用文献
文章目录[隐藏]
- Involvement of natural killer cells in the pathogenesis of endometriosis in patients with pelvic pain 
- Efficacy of NKG2D CAR-T cells with IL-15/IL-15Rα signaling for treating Epstein-Barr virus-associated lymphoproliferative disorder 
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- Targeting osteopontin alleviates endometriosis and inflammation by inhibiting the RhoA/ROS axis and achieves non-invasive in vitro detection via menstrual blood 
- CD19/CD20 dual-targeted chimeric antigen receptor-engineered natural killer cells exhibit improved cytotoxicity against acute lymphoblastic leukemia 
- Lyophilized lymph nodes for improved delivery of chimeric antigen receptor T cells 
Involvement of natural killer cells in the pathogenesis of endometriosis in patients with pelvic pain 
Efficacy of NKG2D CAR-T cells with IL-15/IL-15Rα signaling for treating Epstein-Barr virus-associated lymphoproliferative disorder 
Epithelial cells derived exosomal miR-203a-3p facilitates stromal inflammation of type IIIA chronic prostatitis/chronic pelvic pain syndrome by targeting DUSP5 and increasing MCP-1 generation 
Targeting osteopontin alleviates endometriosis and inflammation by inhibiting the RhoA/ROS axis and achieves non-invasive in vitro detection via menstrual blood 
CD19/CD20 dual-targeted chimeric antigen receptor-engineered natural killer cells exhibit improved cytotoxicity against acute lymphoblastic leukemia 
Lyophilized lymph nodes for improved delivery of chimeric antigen receptor T cells 
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