EK110HSS

Human IL-10 High Sensitivity Standard (人白介素10 高敏 标准品)

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¥180.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：IL10, CSIF, TGIF
  • 种属：人 (Human)
  • 试剂盒：EK110HS
  • 保存：短期4℃保存，长期-20℃保存
  • 运输条件：4℃蓝冰运输

在售SKU:70-EK110HSS, 人白细胞介素10, 白细胞介素10, 白细胞介素, 人, 白介素, 10, 高敏

描述

本产品只包含标准品试剂，如需购买试剂盒请点击下图

商品名

Human IL-10 High Sensitivity Standard (人白介素10 高敏 标准品)
组分

人IL-10高敏标准品
检测方法

双抗夹心法
样本类型

血清，血浆，细胞培养上清及其他生物学样本
板式

管
保存

短期4℃，长期-20℃保存
运输条件

4℃蓝冰运输

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-10抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的IL-10与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-10的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human IL-10 High Sensitivity ELISA Kit (人白介素10 高敏 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 IL10, CSIF, TGIF 靶点信息

IL10 分子靶点信息概述

  • 分子名：IL10, interleukin 10
  • 基因家族：Interleukins
  • 别名：CSIF; TGIF; IL10A; IL-10
  • 全称：cytokine synthesis inhibitory factor; T-cell growth inhibitory factor

IL10 分子靶点综述

白细胞介素10(IL-10)是由2个长178个氨基酸的亚基构成的同源二聚体。在人类中，它由位于1号染色体的IL-10基因编码，由5个外显子组成。IL-10主要由单核细胞分泌，淋巴细胞、II型辅助性T细胞(Th2)、肥大细胞、CD4+CD25+Foxp3+调节性T细胞和部分活化的T细胞和B细胞分泌较少。它对免疫调控和炎症具有多种效应。它下调巨噬细胞的Th1细胞因子、II类MHC抗原和共刺激分子。它还能增强B细胞存活、增殖和抗体分泌。IL-10能阻断NF-κB活性，并参与JAK-STAT信号通路调控。IL-10的免疫抑制特性提示其可能应用于临床，抑制器官移植后的排斥。IL-10还具有强烈的消炎特性。

人 Human IL10 分子靶点信息

  • 分子名：IL10, interleukin 10
  • 别称：
    • CSIF
    • cytokine synthesis inhibitory factor
    • GVHDS
    • IL-10
    • IL10A
    • interleukin-10
    • MGC126450
    • MGC126451
    • T-cell growth inhibitory factor
    • TGIF
  • 基因序列：NCBI_Gene: 3586
  • 蛋白序列：UniProtKB: P22301

人 Human IL10靶点分子功能（预测）

Enables protein dimerization activity. Involved in several processes, including negative regulation of cellular catabolic process; regulation of gene expression; and regulation of signal transduction. Acts upstream of or within several processes, including negative regulation of B cell proliferation; positive regulation of B cell apoptotic process; and positive regulation of signaling receptor activity. Located in extracellular space. Implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Biomarker of several diseases, including autoimmune disease (multiple); diabetes mellitus (multiple); eye disease (multiple); female reproductive organ cancer (multiple); and hematologic cancer (multiple).

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


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  2. Involvement of impaired CD8+ mucosal-associated invariant T cells and myeloid-derived suppressor cells in polycystic ovary syndrome 

  3. Citrus alkaline extracts prevent endoplasmic reticulum stress in type II alveolar epithelial cells to ameliorate pulmonary fibrosis via the ATF3/PINK1 pathway 

  4. Paclitaxel alleviated sepsis-induced acute lung injury by activating MUC1 and suppressing TLR-4/NF-κB pathway 

  5. Mycobacterial PPE13 activates inflammasome by interacting with the NATCH and LRR domains of NLRP3 

  6. Polysaccharides from Dendrobium officinale ameliorate colitis-induced lung injury via inhibiting inflammation and oxidative stress 

  7. miR-374b-5p is increased in deep vein thrombosis and negatively targets IL-10 

  8. Cytokine nanosponges suppressing overactive macrophages and dampening systematic cytokine storm for the treatment of hemophagocytic lymphohistiocytosis 

  9. Effects of the Chinese herbal medicine Hong Huang decoction, on myocardial injury in breast cancer patients who underwent anthracycline-based chemotherapy 

  10. Inhibition of microRNA-92a ameliorates lipopolysaccharide-induced endothelial barrier dysfunction by targeting ITGA5 through the PI3K/Akt signaling pathway in human pulmonary microvascular endothelial cells 

  11. Protective mechanisms of Zanthoxylum bungeanum essential oil on DSS-induced ulcerative colitis in mice based on a colonic mucosal transcriptomic approach 

  12. Vitamin D deficiency is associated with thyroid autoimmunity: results from an epidemiological survey in Tianjin, China 

  13. MiR-146a-5p Mimic Inhibits NLRP3 Inflammasome Downstream Inflammatory Factors and CLIC4 in Neonatal Necrotizing Enterocolitis 

  14. A cross-sectional study reveals a chronic low-grade inflammation in achalasia 

  15. Adoptive immunotherapy using T lymphocytes redirected to glypican-3 for the treatment of lung squamous cell carcinoma 

  16. The Effect of Entecavir Therapy on Immune Status in Chronic Hepatitis B Patients 

  17. Interleukin-21 Inhibits HBV Replication in vitro 

  18. Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 

  19. A novel bispecific antibody targeting CD3 and prolactin receptor (PRLR) against PRLR-expression breast cancer 

  20. MicroRNA 27b-3p Modulates SYK in Pediatric Asthma Induced by Dust Mites 

  21. Cytokine-induced killer cells efficiently kill stem-like cancer cells of nasopharyngeal carcinoma via the NKG2D-ligands recognition 

  22. Vitamin D Deficiency Contributes to the Reduction and Impaired Function of Naïve Regulatory T Cell in Chronic Heart Failure 

  23. Imbalance of Th17, Treg, and helper innate lymphoid cell in the peripheral blood of patients with rheumatoid arthritis 

  24. Cell-free miR-17-5p as a diagnostic biomarker for gastric cancer inhibits dendritic cell maturation 

  25. Human umbilical vein endothelial cells promote the inhibitory activation of CD4+CD25+Foxp3+ regulatory T cells via PD-L1 

  26. Volume kinetics of Ringer's lactate solution in acute inflammatory disease 

  27. Targeting the class IA PI3K isoforms p110α/δ attenuates heart allograft rejection in mice by suppressing the CD4+ T lymphocyte response 

  28. Circulating CD14+CD163+CD206+ M2 Monocytes Are Increased in Patients with Early Stage of Idiopathic Membranous Nephropathy 

  29. Acute Myeloid Leukemia Cells Express ICOS Ligand to Promote the Expansion of Regulatory T Cells 

  30. RNASE2 Mediates Age-Associated B Cell Expansion Through Monocyte Derived IL-10 in Patients With Systemic Lupus Erythematosus 

  31. Relationship between IL-10 and PD-L1 in esophageal carcinoma tissues and IL-10 down-regulates PD-L1 expression via Met signaling pathway 

  32. Myeloid-derived suppressor cell cytokine secretion as prognostic factor in myelodysplastic syndromes 

  33. Physical confinement in alginate cryogels determines macrophage polarization to a M2 phenotype by regulating a STAT-related mRNA transcription pathway 

  34. Circular RNA circWWC3 augments breast cancer progression through promoting M2 macrophage polarization and tumor immune escape via regulating the expression and secretion of IL-4 

  35. Associations of Interleukin 10, Matrix Metallopeptidase 9, and Legumain with Blood Pressure Variability and Neurologic Outcomes in Patients with Ischemic Stroke 

  36. Effects of high serum uric acid levels on oxidative stress levels and semen parameters in male infertile patients 

  37. Increased Tim-3+ monocytes/macrophages are associated with disease severity in patients with IgA nephropathy 

  38. Lipopolysaccharide from biofilm-forming Pseudomonas aeruginosa PAO1 induces macrophage hyperinflammatory responses 

  39. Comparison of morphology, phenotypes and function between cultured human IL‑4‑DC and IFN‑DC 

  40. Tonsillar Microbiome-Derived Lantibiotics Induce Structural Changes of IL-6 and IL-21 Receptors and Modulate Host Immunity 

  41. Changes of Th17/Tc17 and Th17/Treg cells in endometrial carcinoma 

  42. Characterization of CD19+CD24hiCD38hi B cells in Chinese adult patients with atopic dermatitis 

  43. Adhesion-Related Immunomodulatory Activity of the Screened Lactobacillus plantarum from Sichuan Pickle 

  44. Increased Expression of TLR10 in B Cell Subsets Correlates with Disease Activity in Rheumatoid Arthritis 

  45. Shotgun Lipidomics Revealed Altered Profiles of Serum Lipids in Systemic Lupus Erythematosus Closely Associated with Disease Activity 

  46. PD-L1 Is Expressed and Promotes the Expansion of Regulatory T Cells in Acute Myeloid Leukemia 

  47. Deficiency of MST1 in endometriosis related peritoneal macrophages promoted the autophagy of ectopic endometrial stromal cells by IL-10 

  48. Decreased expression of IL-27 in moderate-to-severe psoriasis and its anti-inflammation role in imiquimod-induced psoriasis-like mouse model 

  49. Ulcerative Colitis Is Characterized by a Decrease in Regulatory B Cells 

  50. Expression profile of PU.1 in CD4+T cells from patients with systemic lupus erythematosus 

  51. Changes in peripheral blood cytokines in patients with severe fever with thrombocytopenia syndrome 

  52. Fibrinogen-Like Protein 1 as a Novel Biomarker of Psoriasis Severity 

  53. Comparison and relationship of thyroid hormones, IL-6, IL-10 and albumin as mortality predictors in case-mix critically ill patients 

  54. Glucose Metabolism Reprogramming of Regulatory T Cells in Concanavalin A-Induced Hepatitis 

  55. Potential biomarkers for diagnosis and assessment of disease activity in systemic lupus erythematosus 

  56. Impaired myeloid-derived suppressor cells are associated with recurrent implantation failure: A case-control study 

  57. The clinical study on treatment of CD19-directed chimeric antigen receptor-modified T cells in a case of refractory Richter syndrome 

  58. Distribution of Th17 cells and Foxp3-expressing T cells in tumor-infiltrating lymphocytes in patients with uterine cervical cancer 

  59. Helicobacter pylori induces gastric cancer via down-regulating miR-375 to inhibit dendritic cell maturation 

  60. Tumor-Derived Autophagosomes (DRibbles) Activate Human B Cells to Induce Efficient Antigen-Specific Human Memory T-Cell Responses 

  61. Human bone marrow mesenchymal stem cells-derived exosomes protect against nerve injury via regulating immune microenvironment in neonatal hypoxic-ischemic brain damage model 

  62. Involvement of the Tim-3 Pathway in the Pathogenesis of Pre-Eclampsia 

  63. Regulation of CBL and ESR1 expression by microRNA-22‑3p, 513a-5p and 625-5p may impact the pathogenesis of dust mite-induced pediatric asthma 

  64. Expression of coinhibitory PD-L1 on CD4+CD25+FOXP3+ regulatory T cells is elevated in patients with acute coronary syndrome 

  65. Recognition and killing of cancer stem-like cell population in hepatocellular carcinoma cells by cytokine-induced killer cells via NKG2d-ligands recognition 

  66. Infant immune response to hepatitis B vaccine after fetal exposure to telbivudine 

  67. A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide. 

  68. Modulating macrophage polarization on titanium implant surface by poly(dopamine)-assisted immobilization of IL4 

  69. IgD-activated mast cells induce IgE synthesis in B cells in nasal polyps 

  70. Study on the Mechanism of Improving HIV/AIDS Immune Function with Jian Aikang Concentrated Pill Based on Network Pharmacology Combined with Experimental Validation 

  71. Extracellular vesicles secreted by hypoxia pre-challenged mesenchymal stem cells promote non-small cell lung cancer cell growth and mobility as well as macrophage M2 polarization via miR-21-5p delivery 

  72. Time‑series clustering of cytokine expression after transarterial chemoembolization in patients with hepatocellular carcinoma 

  73. Isopsoralen ameliorates rheumatoid arthritis by targeting MIF 

  74. Oxidative stress leads to reduction of plasmalogen serving as a novel biomarker for systemic lupus erythematosus 

  75. Effect of soothing-liver and nourishing-heart acupuncture on early selective serotonin reuptake inhibitor treatment onset for depressive disorder and related indicators of neuroimmunology: a randomized controlled clinical trial 

  76. IFN-DC Loaded with Autophagosomes containing Virus Antigen is Highly Efficient in Inducing Virus-Specific Human T Cells 

  77. Changes in regulatory B cells and their relationship with rheumatoid arthritis disease activity 

  78. Expression and significance of peripheral myeloid-derived suppressor cells in chronic hepatitis B patients 

  79. Decreased frequency of circulating Th9 cells in patients with chronic hepatitis B infection 

  80. Potential Action Mechanism of Erianin in Relieving MNNG-triggered Chronic Atrophic Gastritis 

  81. Negative impact of maternal depressive symptoms on infancy neurodevelopment: a moderated mediation effect of maternal inflammation 

  82. Spatio-temporally controlled all-in-one hydrogel microspheres for ulcerative colitis treatment based on cocktail strategy 

  83. Effects of continuous negative pressure suction combined with autologous platelet-rich gel on the levels of CRP, IL-6, wound healing and length of stay in clients with diabetic foot 

  84. A citrullinated antigenic vaccine in treatment of autoimmune arthritis 

  85. Monocarboxylate Transporter 4 (Mct4) Facilitates Mycobacterium tuberculosis Survival through NF-κB p65 Mediated Interleukin-10 Production 

  86. Structure and biological activity in vitro of Flagellin and its mutants from Escherichia coli Nissle 1917 

  87. Edible bird's nest alleviates pneumonia caused by tobacco smoke inhalation through the TNFR1/NF-κB/NLRP3 pathway 

  88. 23-Hydroxybetulinic acid attenuates 5-fluorouracil resistance of colorectal cancer by modulating M2 macrophage polarization via STAT6 signaling 

  89. A smart band-aid with temperature and reactive oxygen species dual-responsiveness for synergistic mechanical and pharmacological therapy of chronic wounds 

  90. Poly(rC)-binding protein 1 alleviates neurotoxicity in 6-OHDA-induced SH-SY5Y cells and modulates glial cells in neuroinflammation 

  91. Development and experimental validation of an M2 macrophage and platelet-associated gene signature to predict prognosis and immunotherapy sensitivity in bladder cancer 

  92. Solobacterium moorei promotes the progression of adenomatous polyps by causing inflammation and disrupting the intestinal barrier 

  93. Glycolysis Induced by METTL14 Is Essential for Macrophage Phagocytosis and Phenotype in Cervical Cancer 

  94. Prophylactic treatment with Bacteroides uniformis and Bifidobacterium bifidum counteracts hepatic NK cell immune tolerance in nonalcoholic steatohepatitis induced by high fat diet 

  95. IL4I1 in M2-like macrophage promotes glioma progression and is a promising target for immunotherapy 

  96. Keratinocyte-to-macrophage communication exacerbate psoriasiform dermatitis via LRG1-enriched extracellular vesicles 

  97. Characteristics of Serum Autoantibody Repertoire and Immune Subgroup Variation of Tuberculosis-Associated Obstructive Pulmonary Disease 

  98. Single-cell transcriptomics reveals variations in monocytes and Tregs between gout flare and remission 

  99. BMI-specific inflammatory response to phthalate exposure in early pregnancy: findings from the TMCHESC study 

  100. hPMSCs Regulate the Level of TNF-α and IL-10 in Th1 Cells and Improve Hepatic Injury in a GVHD Mouse Model via CD73/ADO/Fyn/Nrf2 Axis 

  101. CAP2 promotes gastric cancer metastasis by mediating the interaction between tumor cells and tumor-associated macrophages 

  102. α5-nAChR/STAT3/CD47 axis contributed to nicotine-related lung adenocarcinoma progression and immune escape 

  103. A role of NR4A2 in Graves’ disease: regulation of Th17/Treg 

  104. Orosomucoid 1 promotes colorectal cancer progression and liver metastasis by affecting PI3K/AKT pathway and inducing macrophage M2 polarization 

  105. The Effect of an Extract of Sappanwood, Protosappanin A and Protosappanin B on Osteogenesis in Periodontitis 

  106. Blocking IL-6 signaling improves glucose tolerance via SLC39A5-mediated suppression of glucagon secretion 

  107. The enrichment of Arg1+ILC2s and ILCregs facilitates the progression of endometriosis: A preliminary study 

  108. Potentiated effects of lactate receptor GPR81 on immune microenvironment in breast cancer 

  109. Imbalance of follicular regulatory T (Tfr) cells/follicular helper T (Tfh) cells in adult patients with primary immune thrombocytopenia 

  110. Parthenolide alleviates microglia‐mediated neuroinflammation via MAPK/TRIM31/NLRP3 signaling to ameliorate cognitive disorder 

  111. Clonorchis sinensis granulin promotes malignant transformation of human intrahepatic biliary epithelial cells through interaction with M2 macrophages via regulation of STAT3? phosphorylation and the MEK/ERK pathway 

  112. Modified Gegen Qinlian decoction ameliorated ulcerative colitis by attenuating inflammation and oxidative stress and enhancing intestinal barrier function in vivo and in vitro 

  113. MicroRNA-143-3p ameliorates collagen-induced arthritis by polarizing naive CD4+T cells into Treg cells 

  114. Azithromycin attenuates wheezing after pulmonary inflammation through inhibiting histone H3K27me3 hypermethylation mediated by EZH2 

  115. A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide 

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