EK110

Human IL-10 ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：IL10, CSIF, TGIF
  • 种属：人 (Human)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：血清血浆：50 μL；细胞培养上清：100 μL
  • 灵敏度：0.43 pg/mL
  • 检测范围：3.13 pg/mL - 200 pg/mL
  • 回收率：80% - 117%

在售SKU:70-EK110/2-48, 70-EK110/2-96, 人白细胞介素10, 白细胞介素10, 白细胞介素, 10, 白介素

描述

商品名

Human IL-10 ELISA Kit (人白细胞介素10ELISA 试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：3.2% - 5.7%；板间变异系数：2.9% - 8.2%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

血清血浆：50 μL；细胞培养上清：100 μL
灵敏度

0.43 pg/mL
检测范围

3.13 pg/mL - 200 pg/mL
回收率

80% - 117%
平均回收率

1.04
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗IL-10单克隆抗体的96孔聚苯乙烯酶标板
  • 人IL-10冻干标准品
  • IL-10 检测抗体
  • 标准品稀释液
  • 辣根过氧化物酶标记的链霉亲和素
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-10抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-10与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-10的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human IL-10 ELISA Kit (人白细胞介素10ELISA 试剂盒) - 标准曲线
标准曲线

分子信息

概述人 IL10, CSIF, TGIF 靶点信息

IL10 分子靶点信息概述

  • 分子名：IL10, interleukin 10
  • 基因家族：Interleukins
  • 别名：CSIF; TGIF; IL10A; IL-10
  • 全称：cytokine synthesis inhibitory factor; T-cell growth inhibitory factor

IL10 分子靶点综述

白细胞介素10(IL-10)是由2个长178个氨基酸的亚基构成的同源二聚体。在人类中，它由位于1号染色体的IL-10基因编码，由5个外显子组成。IL-10主要由单核细胞分泌，淋巴细胞、II型辅助性T细胞(Th2)、肥大细胞、CD4+CD25+Foxp3+调节性T细胞和部分活化的T细胞和B细胞分泌较少。它对免疫调控和炎症具有多种效应。它下调巨噬细胞的Th1细胞因子、II类MHC抗原和共刺激分子。它还能增强B细胞存活、增殖和抗体分泌。IL-10能阻断NF-κB活性，并参与JAK-STAT信号通路调控。IL-10的免疫抑制特性提示其可能应用于临床，抑制器官移植后的排斥。IL-10还具有强烈的消炎特性。

人 Human IL10 分子靶点信息

  • 分子名：IL10, interleukin 10
  • 别称：
    • CSIF
    • cytokine synthesis inhibitory factor
    • GVHDS
    • IL-10
    • IL10A
    • interleukin-10
    • MGC126450
    • MGC126451
    • T-cell growth inhibitory factor
    • TGIF
  • 基因序列：NCBI_Gene: 3586
  • 蛋白序列：UniProtKB: P22301

人 Human IL10靶点分子功能（预测）

Enables protein dimerization activity. Involved in several processes, including negative regulation of cellular catabolic process; regulation of gene expression; and regulation of signal transduction. Acts upstream of or within several processes, including negative regulation of B cell proliferation; positive regulation of B cell apoptotic process; and positive regulation of signaling receptor activity. Located in extracellular space. Implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Biomarker of several diseases, including autoimmune disease (multiple); diabetes mellitus (multiple); eye disease (multiple); female reproductive organ cancer (multiple); and hematologic cancer (multiple).

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]

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  2. Construction of PD1/CD28 chimeric-switch receptor enhances anti-tumor ability of c-Met CAR-T in gastric cancer 

  3. Involvement of MM cell‑derived exosomes in T lymphocytes immune responses 

  4. Involvement of impaired CD8+ mucosal-associated invariant T cells and myeloid-derived suppressor cells in polycystic ovary syndrome 

  5. Citrus alkaline extracts prevent endoplasmic reticulum stress in type II alveolar epithelial cells to ameliorate pulmonary fibrosis via the ATF3/PINK1 pathway 

  6. Paclitaxel alleviated sepsis-induced acute lung injury by activating MUC1 and suppressing TLR-4/NF-κB pathway 

  7. Wilforlide A ameliorates the progression of rheumatoid arthritis by inhibiting M1 macrophage polarization 

  8. Mycobacterial PPE13 activates inflammasome by interacting with the NATCH and LRR domains of NLRP3 

  9. Platelet-derived growth factor B attenuates lethal sepsis through inhibition of inflammatory responses 

  10. Polysaccharides from Dendrobium officinale ameliorate colitis-induced lung injury via inhibiting inflammation and oxidative stress 

  11. miR-374b-5p is increased in deep vein thrombosis and negatively targets IL-10 

  12. Cytokine nanosponges suppressing overactive macrophages and dampening systematic cytokine storm for the treatment of hemophagocytic lymphohistiocytosis 

  13. Effects of the Chinese herbal medicine Hong Huang decoction, on myocardial injury in breast cancer patients who underwent anthracycline-based chemotherapy 

  14. Inhibition of microRNA-92a ameliorates lipopolysaccharide-induced endothelial barrier dysfunction by targeting ITGA5 through the PI3K/Akt signaling pathway in human pulmonary microvascular endothelial cells 

  15. Protective mechanisms of Zanthoxylum bungeanum essential oil on DSS-induced ulcerative colitis in mice based on a colonic mucosal transcriptomic approach 

  16. Vitamin D deficiency is associated with thyroid autoimmunity: results from an epidemiological survey in Tianjin, China 

  17. MiR-146a-5p Mimic Inhibits NLRP3 Inflammasome Downstream Inflammatory Factors and CLIC4 in Neonatal Necrotizing Enterocolitis 

  18. A cross-sectional study reveals a chronic low-grade inflammation in achalasia 

  19. Adoptive immunotherapy using T lymphocytes redirected to glypican-3 for the treatment of lung squamous cell carcinoma 

  20. The Effect of Entecavir Therapy on Immune Status in Chronic Hepatitis B Patients 

  21. Interleukin-21 Inhibits HBV Replication in vitro 

  22. Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 

  23. A novel bispecific antibody targeting CD3 and prolactin receptor (PRLR) against PRLR-expression breast cancer 

  24. MicroRNA 27b-3p Modulates SYK in Pediatric Asthma Induced by Dust Mites 

  25. Cytokine-induced killer cells efficiently kill stem-like cancer cells of nasopharyngeal carcinoma via the NKG2D-ligands recognition 

  26. Vitamin D Deficiency Contributes to the Reduction and Impaired Function of Naïve Regulatory T Cell in Chronic Heart Failure 

  27. Imbalance of Th17, Treg, and helper innate lymphoid cell in the peripheral blood of patients with rheumatoid arthritis 

  28. High-Dose Aluminum Exposure Further Alerts Immune Phenotype in Aplastic Anemia Patients 

  29. Cell-free miR-17-5p as a diagnostic biomarker for gastric cancer inhibits dendritic cell maturation 

  30. Human umbilical vein endothelial cells promote the inhibitory activation of CD4+CD25+Foxp3+ regulatory T cells via PD-L1 

  31. Volume kinetics of Ringer's lactate solution in acute inflammatory disease 

  32. Targeting the class IA PI3K isoforms p110α/δ attenuates heart allograft rejection in mice by suppressing the CD4+ T lymphocyte response 

  33. Circulating CD14+CD163+CD206+ M2 Monocytes Are Increased in Patients with Early Stage of Idiopathic Membranous Nephropathy 

  34. Acute Myeloid Leukemia Cells Express ICOS Ligand to Promote the Expansion of Regulatory T Cells 

  35. Wnt/β-catenin signaling mediates the abnormal osteogenic and adipogenic capabilities of bone marrow mesenchymal stem cells from chronic graft-versus-host disease patients 

  36. Myeloid-derived suppressor cell cytokine secretion as prognostic factor in myelodysplastic syndromes 

  37. Physical confinement in alginate cryogels determines macrophage polarization to a M2 phenotype by regulating a STAT-related mRNA transcription pathway 

  38. Circular RNA circWWC3 augments breast cancer progression through promoting M2 macrophage polarization and tumor immune escape via regulating the expression and secretion of IL-4 

  39. Associations of Interleukin 10, Matrix Metallopeptidase 9, and Legumain with Blood Pressure Variability and Neurologic Outcomes in Patients with Ischemic Stroke 

  40. Effects of high serum uric acid levels on oxidative stress levels and semen parameters in male infertile patients 

  41. Increased Tim-3+ monocytes/macrophages are associated with disease severity in patients with IgA nephropathy 

  42. Lipopolysaccharide from biofilm-forming Pseudomonas aeruginosa PAO1 induces macrophage hyperinflammatory responses 

  43. Comparison of morphology, phenotypes and function between cultured human IL‑4‑DC and IFN‑DC 

  44. Tonsillar Microbiome-Derived Lantibiotics Induce Structural Changes of IL-6 and IL-21 Receptors and Modulate Host Immunity 

  45. Changes of Th17/Tc17 and Th17/Treg cells in endometrial carcinoma 

  46. Characterization of CD19+CD24hiCD38hi B cells in Chinese adult patients with atopic dermatitis 

  47. Adhesion-Related Immunomodulatory Activity of the Screened Lactobacillus plantarum from Sichuan Pickle 

  48. Increased Expression of TLR10 in B Cell Subsets Correlates with Disease Activity in Rheumatoid Arthritis 

  49. Shotgun Lipidomics Revealed Altered Profiles of Serum Lipids in Systemic Lupus Erythematosus Closely Associated with Disease Activity 

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  51. Deficiency of MST1 in endometriosis related peritoneal macrophages promoted the autophagy of ectopic endometrial stromal cells by IL-10 

  52. Decreased expression of IL-27 in moderate-to-severe psoriasis and its anti-inflammation role in imiquimod-induced psoriasis-like mouse model 

  53. Ulcerative Colitis Is Characterized by a Decrease in Regulatory B Cells 

  54. In vitro neutralization of autocrine IL‑10 affects Op18/stathmin signaling in non‑small cell lung cancer cells 

  55. A long-term anti-inflammation markedly alleviated high-fat diet-induced obesity by repeated administrations of overexpressing IL10 human umbilical cord-derived mesenchymal stromal cells 

  56. Interleukin-10 genetically modified clinical-grade mesenchymal stromal cells markedly reinforced functional recovery after spinal cord injury via directing alternative activation of macrophages 

  57. Expression profile of PU.1 in CD4+T cells from patients with systemic lupus erythematosus 

  58. Changes in peripheral blood cytokines in patients with severe fever with thrombocytopenia syndrome 

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  60. Glucose Metabolism Reprogramming of Regulatory T Cells in Concanavalin A-Induced Hepatitis 

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  70. Helicobacter pylori induces gastric cancer via down-regulating miR-375 to inhibit dendritic cell maturation 

  71. Tumor-Derived Autophagosomes (DRibbles) Activate Human B Cells to Induce Efficient Antigen-Specific Human Memory T-Cell Responses 

  72. Human bone marrow mesenchymal stem cells-derived exosomes protect against nerve injury via regulating immune microenvironment in neonatal hypoxic-ischemic brain damage model 

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  75. Expression of coinhibitory PD-L1 on CD4+CD25+FOXP3+ regulatory T cells is elevated in patients with acute coronary syndrome 

  76. Recognition and killing of cancer stem-like cell population in hepatocellular carcinoma cells by cytokine-induced killer cells via NKG2d-ligands recognition 

  77. Infant immune response to hepatitis B vaccine after fetal exposure to telbivudine 

  78. A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide. 

  79. Modulating macrophage polarization on titanium implant surface by poly(dopamine)-assisted immobilization of IL4 

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  84. Time‑series clustering of cytokine expression after transarterial chemoembolization in patients with hepatocellular carcinoma 

  85. Isopsoralen ameliorates rheumatoid arthritis by targeting MIF 

  86. Oxidative stress leads to reduction of plasmalogen serving as a novel biomarker for systemic lupus erythematosus 

  87. Effect of soothing-liver and nourishing-heart acupuncture on early selective serotonin reuptake inhibitor treatment onset for depressive disorder and related indicators of neuroimmunology: a randomized controlled clinical trial 

  88. IFN-DC Loaded with Autophagosomes containing Virus Antigen is Highly Efficient in Inducing Virus-Specific Human T Cells 

  89. Changes in regulatory B cells and their relationship with rheumatoid arthritis disease activity 

  90. Expression and significance of peripheral myeloid-derived suppressor cells in chronic hepatitis B patients 

  91. Decreased frequency of circulating Th9 cells in patients with chronic hepatitis B infection 

  92. Targeting CD47 and Angiogenesis Demonstrates Effective Anti-Tumor Effect in Bladder Cancer 

  93. Spatio-temporally controlled all-in-one hydrogel microspheres for ulcerative colitis treatment based on cocktail strategy 

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