EK180HS

Human IFN-γ High Sensitivity ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥2,000.00 – ¥3,400.00

  • 分子靶点：IFNG, IFN-γ
  • 种属：人 (Human)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：血清血浆：50 μL；细胞培养上清：100 μL
  • 灵敏度：0.04 pg/mL
  • 检测范围：0.39 pg/mL - 25 pg/mL
  • 回收率：85% - 94%

在售SKU:70-EK180HS-48, 70-EK180HS-96

描述

商品名

Human IFN-γ High Sensitivity ELISA Kit (人γ干扰素高敏 ELISA 试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：4.3% - 5.5%；板间变异系数：4.7% - 7.8%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

血清血浆：50 μL；细胞培养上清：100 μL
灵敏度

0.04 pg/mL
检测范围

0.39 pg/mL - 25 pg/mL
回收率

85% - 94%
平均回收率

0.89
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗IFN-γ单克隆抗体的96孔聚苯乙烯酶标板
  • 人IFN-γ冻干标准品
  • IFN-γ检测抗体
  • 标准品稀释液
  • 辣根过氧化物酶标记的链霉亲和素
  • 信号增强剂浓缩液
  • 信号增强剂稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IFN-γ抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的IFN-γ与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IFN-γ的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human IFN-γ High Sensitivity ELISA Kit (人γ干扰素高敏 ELISA 试剂盒) - 标准曲线
标准曲线

分子信息

概述人 IFNG, IFN-γ 靶点信息

IFNG 分子靶点信息概述

  • 分子名：IFNG, interferon gamma
  • 基因家族：Interferons

IFNG 分子靶点综述

IFN-γ主要由Th1型细胞产生，属于II型细胞因子中干扰素家族中的一员，是一类分泌型糖蛋白，由143个氨基酸组成，相对分子质量是16942。IFN-γ主要由活化的T细胞和NK细胞产生，具有调节机体免疫状态，介导细胞免疫，加强受体介导的吞噬功能，增强巨噬细胞和中性粒细胞的杀伤活性，具有抗病毒感染及抗肿瘤等生物学活性。

人 Human IFNG 分子靶点信息

  • 分子名：IFNG, interferon gamma
  • 别称：
    • IFG
    • IFI
    • IFN-gamma
    • IMD69
    • immune interferon
    • interferon, gamma
  • 基因序列：NCBI_Gene: 3458
  • 蛋白序列：UniProtKB: P01579

人 Human IFNG靶点分子功能（预测）

Predicted to enable cytokine activity. Involved in several processes, including positive regulation of cell death; positive regulation of cell differentiation; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including positive regulation of gene expression; positive regulation of protein phosphorylation; and response to virus. Located in extracellular region. Implicated in several diseases, including ataxia telangiectasia; autoimmune disease (multiple); eczema herpeticum; factor VIII deficiency; and leukemia (multiple). Biomarker of several diseases, including acute retinal necrosis syndrome; allergic rhinitis; autoimmune disease (multiple); nephritis (multiple); and otitis media (multiple).

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. Construction of PD1/CD28 chimeric-switch receptor enhances anti-tumor ability of c-Met CAR-T in gastric cancer 

  2. Involvement of impaired CD8+ mucosal-associated invariant T cells and myeloid-derived suppressor cells in polycystic ovary syndrome 

  3. Role of CD8+ T cell exhaustion in the progression and prognosis of acute respiratory distress syndrome induced by sepsis: a prospective observational study 

  4. Cytokine nanosponges suppressing overactive macrophages and dampening systematic cytokine storm for the treatment of hemophagocytic lymphohistiocytosis 

  5. Inflammatory Cytokines Changed in Patients With Depression Before and After Repetitive Transcranial Magnetic Stimulation Treatment 

  6. Promotion of angiogenesis in vitro by Astragalus polysaccharide via activation of TLR4 signaling pathway 

  7. Vitamin D deficiency is associated with thyroid autoimmunity: results from an epidemiological survey in Tianjin, China 

  8. Original research: Shed antigen-induced blocking effect on CAR-T cells targeting Glypican-3 in Hepatocellular Carcinoma 

  9. A Multistage Subunit Vaccine Effectively Protects Mice Against Primary Progressive Tuberculosis, Latency and Reactivation 

  10. Progesterone-induced blocking factor-mediated Th1/Th2 balance correlates with fetal arrest in women who underwent in vitro fertilization and embryo transfer 

  11. Reducing Hinge Flexibility of CAR-T Cells Prolongs Survival In Vivo With Low Cytokines Release 

  12. Adoptive immunotherapy using T lymphocytes redirected to glypican-3 for the treatment of lung squamous cell carcinoma 

  13. Development of bispecific anti-c-Met/PD-1 diabodies for the treatment of solid tumors and the effect of c-Met binding affinity on efficacy 

  14. Preclinical characterization of a Fab-like CD3/CLDN18.2 XFab® bispecific antibody against solid tumors 

  15. Notch1 signalling controls the differentiation and function of myeloid-derived suppressor cells in systemic lupus erythematosus 

  16. Targeting Wnt Signaling in the Tumor Immune Microenvironment to Enhancing EpCAM CAR T-Cell therapy 

  17. Targeting integrin αvβ3 with indomethacin inhibits patient-derived xenograft tumour growth and recurrence in oesophageal squamous cell carcinoma 

  18. Niraparib exhibits a synergistic anti-tumor effect with PD-L1 blockade by inducing an immune response in ovarian cancer 

  19. Dual Mechanisms of Novel CD73-Targeted Antibody and Antibody–Drug Conjugate in Inhibiting Lung Tumor Growth and Promoting Antitumor Immune-Effector Function 

  20. Construction and expression of Mycobacterium tuberculosis fusion protein AR2 and its immunogenicity in combination with various adjuvants to form vaccine 

  21. Enhanced immunogenicity of the tuberculosis subunit Rv0572c vaccine delivered in DMT liposome adjuvant as a BCG-booster 

  22. Human natural killer cells for targeting delivery of gold nanostars and bimodal imaging directed photothermal/photodynamic therapy and immunotherapy 

  23. Decidua-derived granulocyte macrophage colony-stimulating factor induces polymorphonuclear myeloid-derived suppressor cells from circulating CD15+ neutrophils 

  24. Dynamic changes of innate lymphoid cells in acute ST-segment elevation myocardial infarction and its association with clinical outcomes 

  25. Long Noncoding RNA and Circular RNA Expression Profiles of Monocyte-Derived Dendritic Cells in Autoimmune Hepatitis 

  26. The Effect of Entecavir Therapy on Immune Status in Chronic Hepatitis B Patients 

  27. Interleukin-21 Inhibits HBV Replication in vitro 

  28. Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 

  29. A novel bispecific antibody targeting CD3 and prolactin receptor (PRLR) against PRLR-expression breast cancer 

  30. Elevated Peripheral Frequencies of Th22 Cells: A Novel Potent Participant in Obesity and Type 2 Diabetes 

  31. MicroRNA 27b-3p Modulates SYK in Pediatric Asthma Induced by Dust Mites 

  32. Cytokine-induced killer cells efficiently kill stem-like cancer cells of nasopharyngeal carcinoma via the NKG2D-ligands recognition 

  33. JAK-STAT Domain Enhanced MUC1-CAR-T Cells Induced Esophageal Cancer Elimination 

  34. Chimeric cytokine receptor enhancing PSMA-CAR-T cell-mediated prostate cancer regression 

  35. Treatment of hepatocellular carcinoma with a GPC3-targeted bispecific T cell engager 

  36. Development of GPC3 and EGFR-dual-targeting chimeric antigen receptor-T cells for adoptive T cell therapy 

  37. Selective Targeting of Glioblastoma with EGFRvIII/EGFR Bitargeted Chimeric Antigen Receptor T Cell 

  38. Claudin18.2-Specific Chimeric Antigen Receptor Engineered T Cells for the Treatment of Gastric Cancer 

  39. Treatment Efficacy of Chuang Ling Ye, a Traditional Chinese Herbal Medicine Compound, on Idiopathic Granulomatous Mastitis: A Randomized Controlled Trial 

  40. Original research: Switch receptor T3/28 improves long-term persistence and antitumor efficacy of CAR-T cells 

  41. CD4+/CD8+ mucosa-associated invariant T cells foster the development of endometriosis: a pilot study 

  42. Vitamin D Deficiency Contributes to the Reduction and Impaired Function of Naïve Regulatory T Cell in Chronic Heart Failure 

  43. Simultaneous blocking of CD47 and PD-L1 increases innate and adaptive cancer immune responses and cytokine release 

  44. A novel long-term intravenous combined with local treatment with human amnion-derived mesenchymal stem cells for a multidisciplinary rescued uremic calciphylaxis patient and the underlying mechanism 

  45. Inverse relationship between toxic shock syndrome toxin-1 antibodies and interferon-γ and interleukin-6 in peripheral blood mononuclear cells from patients with pediatric tonsillitis caused by Staphylococcus aureus 

  46. Differentiation of T-helper cells in distinct phases of atopic dermatitis involves Th1/Th2 and Th17/Treg 

  47. Immunomodulatory effects of platelets on the severity of hand, foot, and mouth disease infected with enterovirus 71 

  48. Imbalance of Th17, Treg, and helper innate lymphoid cell in the peripheral blood of patients with rheumatoid arthritis 

  49. The Combined Use of Cytokine-Induced Killer Cells and Cyclosporine A for the Treatment of Aplastic Anemia in a Mouse Model 

  50. Engineered IL-7 Receptor Enhances the Therapeutic Effect of AXL-CAR-T Cells on Triple-Negative Breast Cancer 

  51. Nanobody-based CAR T cells targeting intracellular tumor antigens 

  52. A PD-1 peptide antagonist exhibits potent anti-tumor and immune regulatory activity 

  53. Design and Synthesis of A PD-1 Binding Peptide and Evaluation of Its Anti-Tumor Activity 

  54. Development of T Cells Redirected to Glypican-3 for the Treatment of Hepatocellular Carcinoma 

  55. Innate lymphoid cell disturbance with increase in ILC1 in systemic lupus erythematosus 

  56. The preparation of N-IgY targeting SARS-CoV-2 and its immunomodulation to IFN-γ production in vitro 

  57. MicroRNA-34a promotes MICB expression in hepatocytes 

  58. Antitumor effect of IL-12 gene-modified bone marrow mesenchymal stem cells combined with Fuzheng Yiliu decoction in an in vivo glioma nude mouse model 

  59. B7-H3 on breast cancer cell MCF7 inhibits IFN-γ release from tumour-infiltrating T cells 

  60. Generation of hypoimmunogenic human pluripotent stem cells via expression of membrane-bound and secreted β2m-HLA-G fusion proteins 

  61. HOXA11-AS1 Promotes PD-L1-Mediated Immune Escape and Metastasis of Hypopharyngeal Carcinoma by Facilitating PTBP1 and FOSL1 Association 

  62. Increased AHR Transcripts Correlate With Pro-inflammatory T-Helper Lymphocytes Polarization in Both Metabolically Healthy Obesity and Type 2 Diabetic Patients 

  63. Identification of Robust Biomarkers for Early Predicting Efficacy of Subcutaneous Immunotherapy in Children With House Dust Mite-Induced Allergic Rhinitis by Multiple Cytokine Profiling 

  64. High-Dose Aluminum Exposure Further Alerts Immune Phenotype in Aplastic Anemia Patients 

  65. Targeting the class IA PI3K isoforms p110α/δ attenuates heart allograft rejection in mice by suppressing the CD4+ T lymphocyte response 

  66. Tonsillar Microbiome-Derived Lantibiotics Induce Structural Changes of IL-6 and IL-21 Receptors and Modulate Host Immunity 

  67. Changes in peripheral blood cytokines in patients with severe fever with thrombocytopenia syndrome 

  68. Participation of Increased Circulating MAIT Cells in Lung Cancer: a Pilot Study 

  69. Impaired myeloid-derived suppressor cells are associated with recurrent implantation failure: A case-control study 

  70. Regulation of CBL and ESR1 expression by microRNA-22‑3p, 513a-5p and 625-5p may impact the pathogenesis of dust mite-induced pediatric asthma 

  71. Changes in circulating microRNA-126 levels are associated with immune imbalance in children with acute asthma 

  72. ILC2s Induce Adaptive Th2-Type Immunity in Acute Exacerbation of Chronic Obstructive Pulmonary Disease 

  73. Discovery of modulators for the PD-1/PD-L1 interaction by molecular simulation and bioassay 

  74. Hyperbaric Oxygen Alleviates the Inflammatory Response Induced by LPS Through Inhibition of NF-κB/MAPKs-CCL2/CXCL1 Signaling Pathway in Cultured Astrocytes 

  75. Increased antitumor activities of glypican-3-specific chimeric antigen receptor-modified T cells by coexpression of a soluble PD1–CH3 fusion protein 

  76. Significantly increased anti-tumor activity of carcinoembryonic antigen-specific chimeric antigen receptor T cells in combination with recombinant human IL-12 

  77. Antigen presentation of the Oct4 and Sox2 peptides by CD154-activated B lymphocytes enhances the killing effect of cytotoxic T lymphocytes on tumor stem-like cells derived from cisplatin-resistant lung cancer cells 

  78. Accurate control of dual-receptor-engineered T cell activity through a bifunctional anti-angiogenic peptide 

  79. Armored Inducible Expression of IL-12 Enhances Antitumor Activity of Glypican-3–Targeted Chimeric Antigen Receptor–Engineered T Cells in Hepatocellular Carcinoma 

  80. Recognition and killing of cancer stem-like cell population in hepatocellular carcinoma cells by cytokine-induced killer cells via NKG2d-ligands recognition 

  81. Efficacy and safety of low-dose interleukin-2 in combination with methotrexate in patients with active rheumatoid arthritis: a randomized, double-blind, placebo-controlled phase 2 trial 

  82. Infant immune response to hepatitis B vaccine after fetal exposure to telbivudine 

  83. IFN-γ and TNF-α aggravate endothelial damage caused by CD123-targeted CAR T cell 

  84. A novel membrane-bound interleukin-2 promotes NK-92 cell persistence and anti-tumor activity 

  85. Development of T cells carrying two complementary chimeric antigen receptors against glypican-3 and asialoglycoprotein receptor 1 for the treatment of hepatocellular carcinoma 

  86. EGLIF-CAR-T Cells Secreting PD-1 Blocking Antibodies Significantly Mediate the Elimination of Gastric Cancer 

  87. Secretion of human soluble programmed cell death protein 1 by chimeric antigen receptor-modified T cells enhances anti-tumor efficacy 

  88. Enhanced Safety and Antitumor Efficacy of Switchable Dual Chimeric Antigen Receptor-Engineered T Cells against Solid Tumors through a Synthetic Bifunctional PD-L1-Blocking Peptide 

  89. A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide. 

  90. Interleukin-25 is upregulated in patients with systemic lupus erythematosus and ameliorates murine lupus by inhibiting inflammatory cytokine production 

  91. Expression and significance of peripheral myeloid-derived suppressor cells in chronic hepatitis B patients 

  92. Overexpression of early T cell differentiation-specific transcription factors transforms the terminally differentiated effector T cells into less differentiated state 

  93. Distinct roles of programmed death ligand 1 alternative splicing isoforms in colorectal cancer 

  94. Metabolic Reprogramming of CD4+ T Cells by Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuates Autoimmune Hepatitis Through Mitochondrial Protein Transfer 

  95. A nanobody-guided multifunctional T cell engager promotes strong anti-tumor responses via synergistic immuno-photothermal effects 

  96. Blocking the PD-1 signal transduction by occupying the phosphorylated ITSM recognition site of SHP-2 

  97. A citrullinated antigenic vaccine in treatment of autoimmune arthritis 

  98. Regulation of the PD-1/PD-L1 Axis and NK Cell Dysfunction by Exosomal miR-552-5p in Gastric Cancer 

  99. miR-744-5p promotes T-cell differentiation via inhibiting STK11 

  100. Self-reinforcing nano-spearhead drives the efficacy of CAR-T cells against progressive triple negative breast cancer 

  101. circATAD2 mitigates CD8+ T cells antitumor immune surveillance in breast cancer via IGF2BP3/m6A/PD-L1 manner 

  102. CD19/CD20 dual-targeted chimeric antigen receptor-engineered natural killer cells exhibit improved cytotoxicity against acute lymphoblastic leukemia 

  103. Enhanced T cell immune activity mediated by Drp1 promotes the efficacy of PD-1 inhibitors in treating lung cancer 

  104. Mnox Nanoenzyme Armed CAR-NK Cells Enhance Solid Tumor Immunotherapy by Alleviating the Immunosuppressive Microenvironment 

  105. Periodontitis aggravates COPD through the activation of γδ T cell and M2 macrophage 

  106. Prophylactic treatment with Bacteroides uniformis and Bifidobacterium bifidum counteracts hepatic NK cell immune tolerance in nonalcoholic steatohepatitis induced by high fat diet 

  107. Characteristics of Serum Autoantibody Repertoire and Immune Subgroup Variation of Tuberculosis-Associated Obstructive Pulmonary Disease 

  108. (–)-Syringaresinol Attenuates Ulcerative Colitis By Improving Intestinal Epithelial Barrier Function And Inhibiting Inflammatory Responses 

  109. Fe-curcumin nanozyme-mediated immunosuppression and anti-inflammation in experimental autoimmune uveitis 

  110. Single-cell transcriptomics reveals variations in monocytes and Tregs between gout flare and remission 

  111. Develop a PD-1-blockade peptide to reinvigorate T-cell activity and inhibit tumor progress 

  112. N6-methyladenosine reader protein IGF2BP1 suppresses CD8?+?T cells-mediated tumor cytotoxicity and apoptosis in colon cancer 

  113. N6-Methyladenosine (m6A) Reader IGF2BP1 Accelerates Gastric Cancer Development and Immune Escape by Targeting PD-L1 

  114. Targeting and cytotoxicity of chimeric antigen receptor T cells grafted with PD1 extramembrane domain 

  115. A dendritic/tumor fusion cell vaccine enhances efficacy of nanobody-based CAR-T cells against solid tumor 

  116. Novel Benzimidazoles as Potent Small-Molecule Inhibitors and Degraders of V-Domain Ig Suppressor of T-Cell Activation (VISTA) 

  117. The RNA-Splicing Ligase RTCB Promotes Influenza A Virus Replication by Suppressing Innate Immunity via Interaction with RNA Helicase DDX1 

  118. CD24 is a novel target of chimeric antigen receptor T cells for the treatment of triple negative breast cancer 

  119. Novaferon gene modification promotes NK92 cell anti-tumor activity 

  120. Potential salivary and serum biomarkers for burning mouth syndrome and their relationship with anxiety/depression 

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