Human HSP27/HSPB1 Standard (人热休克蛋白 (HSP) 标准品)
¥280.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
描述
文章目录[隐藏]
本产品只包含标准品试剂,如需购买试剂盒请点击下图
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- EK1244
- ELISA试剂盒
Human HSP27/HSPB1 ELISA Kit检测试剂盒(酶联免疫吸附法)
- ¥1,600.00 – ¥2,650.00
商品名 |
Human HSP27/HSPB1 Standard (人热休克蛋白 (HSP) 标准品) |
---|---|
组分 |
人HSP27/HSPB1标准品 |
检测方法 |
双抗夹心法 |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
板式 |
管 |
保存 |
短期4℃,长期-20℃保存 |
运输条件 |
4℃蓝冰运输 |
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人HSP27预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和辣根过氧化物酶标记的检测抗体,经过孵育,样本中存在的HSP27与固相抗体和检测抗体结合。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中HSP27的浓度成正比。加入终止液终止反应,在450 nm波长 (参考波长570 - 630 nm)测定吸光度值。
分子信息
HSPB1 分子靶点信息概述
- 分子名:HSPB1, heat shock protein family B (small) member 1
- 基因家族:Small heat shock proteins
- 别名:HSP27; HSP28; Hs.76067; Hsp25; CMT2F
- 全称:heat shock 27kD protein 1; heat shock 27kDa protein 1
HSPB1 分子靶点综述
热休克蛋白(HSP)是一组高度保守的应激蛋白,主要功能是作为分子伴侣,并参与多种生物学过程包括耐热性、抑制细胞凋亡、调节细胞发育和细胞分化、以及信号转导。 HSP27,又名HSPB1,是小分子热休克蛋白组中的分子伴侣。它存在于许多细胞类型中,尤其是所有类型的肌细胞,在细胞分化和发育的不同阶段过度表达。HSP27参与细胞凋亡信号途径,抑制procaspase-9的活化。此外,HSP27可保护肌动蛋白丝免受破碎,还可激活蛋白酶。HSP27可能是一个潜在的诊断标志物,高水平的HSP27存在于乳腺癌患者的血清中。磷酸化的HSP27增加人类前列腺癌细胞的浸润、促进细胞增殖并抑制Fas诱导的细胞凋亡。
人 Human HSPB1 分子靶点信息
- 分子名:HSPB1, heat shock protein family B (small) member 1
- 别称:
- 28 kDa heat shock protein
- CMT2F
- DKFZp586P1322
- epididymis secretory protein Li 102
- estrogen-regulated 24 kDa protein
- heat shock 27 kDa protein
- heat shock 27kD protein 1
- heat shock 27kDa protein 1
- heat shock protein beta-1
- HEL-S-102
- HMN2B
- HS.76067
- HSP 27
- Hsp25
- HSP27
- HSP28
- putative heat shock protein family B member 1 isoform
- SRP27
- stress-responsive protein 27
- 基因序列:NCBI_Gene: 3315
- 蛋白序列:UniProtKB: P04792
人 Human HSPB1靶点分子功能(预测)
Enables several functions, including protein folding chaperone; protein homodimerization activity; and protein kinase binding activity. Involved in several processes, including anterograde axonal protein transport; positive regulation of angiogenesis; and positive regulation of endothelial cell migration. Located in cytoplasm and nucleus. Implicated in Charcot-Marie-Tooth disease axonal type 2F; distal hereditary motor neuropathy type 2B; pancreatic cancer; and sarcoma. Biomarker of focal segmental glomerulosclerosis and rheumatoid arthritis.
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
- 血清OR血浆,哪个是ELISA的菜 
- ELISA通关必备丨数据篇丨标准曲线不佳 
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- ELISA通关必备丨操作篇丨溶解与稀释标准品 
- ELISA通关必备丨样本篇丨不常见样本 
- ELISA通关必备丨样本篇丨常见样本丨细胞 
- ELISA通关必备丨如何选择试剂盒 
- ELISA通关必备丨基础知识 
- 真?假?ELISA试剂盒选择要小心 
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- 叮!联科向您投递了个ELISA实验操作干货包,请查收~ 
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- 查看更多ELISA操作相关问题
ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
文章目录[隐藏]
- Bmal1 Regulates the Redox Rhythm of HSPB1, and Homooxidized HSPB1 Attenuates the Oxidative Stress Injury of Cardiomyocytes 
- HSPB1 Regulates Autophagy and Apoptosis in Vascular Smooth Muscle Cells in Arteriosclerosis Obliterans 
- Quantitative assessment of serum heat shock protein 27 for the diagnosis of epithelial ovarian cancer using targeted proteomics coupled with immunoaffinity enrichment 
Bmal1 Regulates the Redox Rhythm of HSPB1, and Homooxidized HSPB1 Attenuates the Oxidative Stress Injury of Cardiomyocytes 
HSPB1 Regulates Autophagy and Apoptosis in Vascular Smooth Muscle Cells in Arteriosclerosis Obliterans 
Quantitative assessment of serum heat shock protein 27 for the diagnosis of epithelial ovarian cancer using targeted proteomics coupled with immunoaffinity enrichment 
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