Human HGF Standard (人肝细胞生长因子 标准品)
¥180.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
描述
文章目录[隐藏]
本产品只包含标准品试剂,如需购买试剂盒请点击下图
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- EK1H01
- ELISA试剂盒
Human HGF ELISA Kit检测试剂盒(酶联免疫吸附法)
- ¥1,600.00 – ¥2,650.00
商品名 |
Human HGF Standard (人肝细胞生长因子 标准品) |
---|---|
组分 |
人肝细胞生长因子标准品 |
检测方法 |
双抗夹心法 |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
板式 |
管 |
保存 |
短期4℃,长期-20℃保存 |
运输条件 |
4℃蓝冰运输 |
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人HGF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的HGF与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中HGF的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长5632 nm)测定吸光度值。
分子信息
HGF 分子靶点信息概述
- 分子名:HGF, hepatocyte growth factor
- 别名:SF; F-TCF; HGFB; HPTA
- 曾用名:DFNB39
- 全称:hepatopoietin A; fibroblast-derived tumor cytotoxic factor; scatter factor; lung fibroblast-derived mitogen; deafness, autosomal recessive 39; hepatocyte growth factor (hepapoietin A; scatter factor)
HGF 分子靶点综述
肝细胞生长因子(HGF)是旁分泌性细胞生长、运动和形态发生的因子,由间充质细胞分泌产生,可作为大部分上皮来源细胞的多功能细胞因子。HGF以单链形式存在,是一个带有29个氨基酸信号序列和25个氨基酸前体序列的728个氨基酸残基前肽。HGF结合原癌基因c-Met受体后就会激活酪氨酸激酶信号级联,进而调节细胞的生长、运动和形态发生。它在血管生成、肿瘤发生和组织再生中起核心作用。目前正在测试人HGF质粒DNA治疗心肌细胞是否可作为冠状动脉疾病和心肌梗死后心脏损伤的一种潜在治疗。HGF也可作为基孔肯雅病毒诱导的关节痛的预后指示。高水平的HGF与与快速康复有关。
人 Human HGF 分子靶点信息
- 分子名:HGF, hepatocyte growth factor
- 别称:
- deafness, autosomal recessive 39
- DFNB39
- F-TCF
- fibroblast-derived tumor cytotoxic factor
- hepatocyte growth factor (hepapoietin A, scatter factor)
- hepatopoeitin-A
- hepatopoietin-A
- HGFB
- HPTA
- lung fibroblast-derived mitogen
- SF
- 基因序列:NCBI_Gene: 3082
- 蛋白序列:UniProtKB: P14210
人 Human HGF靶点分子功能(预测)
Enables chemoattractant activity and identical protein binding activity. Involved in several processes, including cellular response to hepatocyte growth factor stimulus; negative regulation of programmed cell death; and positive regulation of nitrogen compound metabolic process. Acts upstream of or within regulation of branching involved in salivary gland morphogenesis by mesenchymal-epithelial signaling. Located in membrane. Implicated in several diseases, including artery disease (multiple); autosomal recessive nonsyndromic deafness 39; eye disease (multiple); neuropathy (multiple); and scleroderma (multiple). Biomarker of several diseases, including artery disease (multiple); biliary tract disease (multiple); myositis (multiple); retinal disease (multiple); and rheumatoid arthritis (multiple).
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
- 血清OR血浆,哪个是ELISA的菜 
- ELISA通关必备丨数据篇丨标准曲线不佳 
- ELISA通关必备丨操作篇丨常见问题及解决方案 
- ELISA通关必备丨操作篇丨溶解与稀释标准品 
- ELISA通关必备丨样本篇丨不常见样本 
- ELISA通关必备丨样本篇丨常见样本丨细胞 
- ELISA通关必备丨如何选择试剂盒 
- ELISA通关必备丨基础知识 
- 真?假?ELISA试剂盒选择要小心 
- ELISA常见类型一 | 双抗夹心法,你要的都在这里! 
- ELISA常见类型二 | 竞争法,五分钟搞定! 
- 叮!联科向您投递了个ELISA实验操作干货包,请查收~ 
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- 【视频】ELISA实验原理与常见问题分析 
- 查看更多ELISA操作相关问题
ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
文章目录[隐藏]
- Human Umbilical Cord-Derived Mesenchymal Stem Cells Ameliorate Skin Aging of Nude Mice Through Autophagy-Mediated Anti-Senescent Mechanism 
- SOX9 in prostate cancer is upregulated by cancer-associated fibroblasts to promote tumor progression through HGF/c-Met-FRA1 signaling 
- Near-infrared light-controllable MXene hydrogel for tunable on-demand release of therapeutic proteins 
- Human umbilical cord MSC‑derived hepatocyte growth factor enhances autophagy in AOPP‑treated HK‑2 cells 
- Establishment of Functional Liver Spheroids From Human Hepatocyte-Derived Liver Progenitor-Like Cells for Cell Therapy 
- Soluble interleukin-27 receptor alpha is a valuable prognostic biomarker for acute graft-versus-host disease after allogeneic haematopoietic stem cell transplantation 
- G6PD-NF-κB-HGF Signal in Gastric Cancer-Associated Mesenchymal Stem Cells Promotes the Proliferation and Metastasis of Gastric Cancer Cells by Upregulating the Expression of HK2 
- Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 
- A novel long-term intravenous combined with local treatment with human amnion-derived mesenchymal stem cells for a multidisciplinary rescued uremic calciphylaxis patient and the underlying mechanism 
- Transcription Factor SOX18 Promotes Clear Cell Renal Cell Carcinoma Progression and Alleviates Cabozantinib-Mediated Inhibitory Effects 
- Microvesicles mediate sorafenib resistance in liver cancer cells through attenuating p53 and enhancing FOXM1 expression 
- Polygonatum kingianum saponins delay cellular senescence through SASP down-regulation and prolong the healthy lifespan of Caenorhabditis elegans by activating sir-2.1/autophagy 
- Stem cell-derived hepatocyte therapy using versatile biomimetic nanozyme incorporated nanofiber-reinforced decellularized extracellular matrix hydrogels for the treatment of acute liver failure 
- Effect of fermentation modification on the physicochemical characteristics and anti-aging related activities of Polygonatum kingianum polysaccharides 
- Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 
- Soluble Interleukin-27 Receptor Alpha Is a Valuable Prognostic Biomarker for Acute Graft-Versus-Host Disease after Allogeneic Haematopoietic Stem Cell Transplantation 
Human Umbilical Cord-Derived Mesenchymal Stem Cells Ameliorate Skin Aging of Nude Mice Through Autophagy-Mediated Anti-Senescent Mechanism 
SOX9 in prostate cancer is upregulated by cancer-associated fibroblasts to promote tumor progression through HGF/c-Met-FRA1 signaling 
Near-infrared light-controllable MXene hydrogel for tunable on-demand release of therapeutic proteins 
Human umbilical cord MSC‑derived hepatocyte growth factor enhances autophagy in AOPP‑treated HK‑2 cells 
Establishment of Functional Liver Spheroids From Human Hepatocyte-Derived Liver Progenitor-Like Cells for Cell Therapy 
Soluble interleukin-27 receptor alpha is a valuable prognostic biomarker for acute graft-versus-host disease after allogeneic haematopoietic stem cell transplantation 
G6PD-NF-κB-HGF Signal in Gastric Cancer-Associated Mesenchymal Stem Cells Promotes the Proliferation and Metastasis of Gastric Cancer Cells by Upregulating the Expression of HK2 
Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 
A novel long-term intravenous combined with local treatment with human amnion-derived mesenchymal stem cells for a multidisciplinary rescued uremic calciphylaxis patient and the underlying mechanism 
Transcription Factor SOX18 Promotes Clear Cell Renal Cell Carcinoma Progression and Alleviates Cabozantinib-Mediated Inhibitory Effects 
Microvesicles mediate sorafenib resistance in liver cancer cells through attenuating p53 and enhancing FOXM1 expression 
Polygonatum kingianum saponins delay cellular senescence through SASP down-regulation and prolong the healthy lifespan of Caenorhabditis elegans by activating sir-2.1/autophagy 
Stem cell-derived hepatocyte therapy using versatile biomimetic nanozyme incorporated nanofiber-reinforced decellularized extracellular matrix hydrogels for the treatment of acute liver failure 
Effect of fermentation modification on the physicochemical characteristics and anti-aging related activities of Polygonatum kingianum polysaccharides 
- 腹腔上清液
Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 
Soluble Interleukin-27 Receptor Alpha Is a Valuable Prognostic Biomarker for Acute Graft-Versus-Host Disease after Allogeneic Haematopoietic Stem Cell Transplantation 
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