Human CXCL2/GRO-β ELISA Kit 检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:CXCL2, MIP-2a, GRO2, GROb
- 种属:人 (Human)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:10 μL
- 灵敏度:0.53 pg/mL
- 检测范围:4.69 pg/mL - 300 pg/mL
- 回收率:85% - 110%
在售SKU:70-EK1264-48, 70-EK1264-96
描述
商品名 |
Human CXCL2/GRO-β ELISA Kit 检测试剂盒(酶联免疫吸附法) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:4.8% - 5.0%;板间变异系数:3.8% - 4.7% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
10 μL |
灵敏度 |
0.53 pg/mL |
检测范围 |
4.69 pg/mL - 300 pg/mL |
回收率 |
85% - 110% |
平均回收率 |
0.99 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人GRO-β抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的GRO-β与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中GRO-β的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
CXCL2 分子靶点信息概述
- 分子名:CXCL2, C-X-C motif chemokine ligand 2
- 基因家族:Chemokine ligands
- 别名:SCYB2; GROb; MIP-2a; MGSA-b; CINC-2a
- 曾用名:GRO2
- 全称:GRO2 oncogene; chemokine (C-X-C motif) ligand 2
CXCL2 分子靶点综述
人生长调节致癌基因β (GRO-β)也称为 CXCL2,是 CXC 蛋白趋化因子家族成员之一。GRO-β 在小鼠中也称为 MIP-2,在大鼠中称为 CINC-3。人 GRO-β由 107 个氨基酸(aa)构成,其分子量约 为 11 kDa。GRO-β是由炎症部位的多种细胞(包括单核细胞和巨噬细胞)分泌,对粒细胞包括中性粒 细胞具有趋化作用。GRO-β在炎症反应和损伤修复过程中起着重要作用。研究逐渐发现 GRO 为一种原癌基因。GRO-β作为表达产物在血管新生、肿瘤形成转移和肿瘤与免疫细胞相互作用中起到关键作用。
人 Human CXCL2 分子靶点信息
- 分子名:CXCL2, C-X-C motif chemokine ligand 2
- 别称:
- C-X-C motif chemokine 2
- chemokine (C-X-C motif) ligand 2
- CINC-2a
- gro-beta
- GRO2
- GRO2 oncogene
- GROb
- growth-regulated protein beta
- macrophage inflammatory protein 2-alpha
- melanoma growth stimulatory activity beta
- MGSA beta
- MGSA-b
- MIP-2a
- MIP2
- MIP2-alpha
- MIP2A
- SCYB2
- 基因序列:NCBI_Gene: 2920
- 蛋白序列:UniProtKB: P19875
人 Human CXCL2靶点分子功能(预测)
Predicted to enable CXCR chemokine receptor binding activity and chemokine activity. Involved in antimicrobial humoral immune response mediated by antimicrobial peptide; killing of cells of other organism; and response to molecule of bacterial origin. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Biomarker of COVID-19 and cystic fibrosis.
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
- 血清OR血浆,哪个是ELISA的菜 
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- ELISA通关必备丨样本篇丨不常见样本 
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- ELISA通关必备丨如何选择试剂盒 
- ELISA通关必备丨基础知识 
- 真?假?ELISA试剂盒选择要小心 
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- 查看更多ELISA操作相关问题
ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
文章目录[隐藏]
- Macrophage polarization toward M1 phenotype through NF-κB signaling in patients with Behçet’s disease 
- Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 
- Alteration in the Cytokine Secretion of Bone Marrow Stromal Cells from Patients with Chronic Myelomonocytic Leukemia Contribute to Impaired Hematopoietic Supportive Activity 
- PGF2α facilitates pathological retinal angiogenesis by modulating endothelial FOS-driven ELR+ CXC chemokine expression 
- Hypoxia-CXCL6 axis affects arteriolar niche remodeling in acute myeloid leukemia. 
- Cancer-associated adipocytes promote the invasion and metastasis in breast cancer through LIF/CXCLs positive feedback loop 
- Cisplatin-stimulated macrophages promote ovarian cancer migration via the CCL20-CCR6 axis 
- Deciphering m6A methylation in monocyte-mediated cardiac fibrosis and monocyte-hitchhiked erythrocyte microvesicle biohybrid therapy 
- The role of CXCL2-mediated crosstalk between tumor cells and macrophages in Fusobacterium nucleatum-promoted oral squamous cell carcinoma progression 
- Salmonella spvC gene suppresses macrophage/neutrophil antibacterial defense mediated by gasdermin D 
- Single-cell transcriptomes reveal a molecular link between diabetic kidney and retinal lesions 
- An inflammatory checkpoint generated by IL1RN splicing offers therapeutic opportunity for KRAS mutant intrahepatic cholangiocarcinoma 
- CSF2 upregulates CXCL3 expression in adipocytes to promote metastasis of breast cancer via the FAK signaling pathway 
Macrophage polarization toward M1 phenotype through NF-κB signaling in patients with Behçet’s disease 
Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 
Alteration in the Cytokine Secretion of Bone Marrow Stromal Cells from Patients with Chronic Myelomonocytic Leukemia Contribute to Impaired Hematopoietic Supportive Activity 
PGF2α facilitates pathological retinal angiogenesis by modulating endothelial FOS-driven ELR+ CXC chemokine expression 
Hypoxia-CXCL6 axis affects arteriolar niche remodeling in acute myeloid leukemia. 
Cancer-associated adipocytes promote the invasion and metastasis in breast cancer through LIF/CXCLs positive feedback loop 
Cisplatin-stimulated macrophages promote ovarian cancer migration via the CCL20-CCR6 axis 
Deciphering m6A methylation in monocyte-mediated cardiac fibrosis and monocyte-hitchhiked erythrocyte microvesicle biohybrid therapy 
The role of CXCL2-mediated crosstalk between tumor cells and macrophages in Fusobacterium nucleatum-promoted oral squamous cell carcinoma progression 
Salmonella spvC gene suppresses macrophage/neutrophil antibacterial defense mediated by gasdermin D 
Single-cell transcriptomes reveal a molecular link between diabetic kidney and retinal lesions 
An inflammatory checkpoint generated by IL1RN splicing offers therapeutic opportunity for KRAS mutant intrahepatic cholangiocarcinoma 
CSF2 upregulates CXCL3 expression in adipocytes to promote metastasis of breast cancer via the FAK signaling pathway 
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