EK163

Human GM-CSF ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:CSF2, GM-CSF
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:20 μL
  • 灵敏度:4.76 pg/mL
  • 检测范围:1.56 pg/mL - 100 pg/mL
  • 回收率:97% - 110%

在售SKU:70-EK163-48, 70-EK163-96


描述

商品名

Human GM-CSF ELISA Kit (人粒-巨噬细胞集落刺激因子 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:2.5% - 2.9%;板间变异系数:3.7% - 4.9%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

20 μL

灵敏度

4.76 pg/mL

检测范围

1.56 pg/mL - 100 pg/mL

回收率

97% - 110%

平均回收率

1.04

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗GM-CSF单克隆抗体的96孔聚苯乙烯酶标板
  • 人GM-CSF冻干标准品
  • GM-CSF检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人GM-CSF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的GM-CSF与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中GM-CSF的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human GM-CSF ELISA Kit (人粒-巨噬细胞集落刺激因子 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 CSF2, GM-CSF 靶点信息

CSF2 分子靶点信息概述

  • 分子名:CSF2, colony stimulating factor 2
  • 别名:GM-CSF; GMCSF
  • 全称:sargramostim; molgramostim; granulocyte-macrophage colony stimulating factor; colony stimulating factor 2 (granulocyte-macrophage)

CSF2 分子靶点综述

粒细胞-巨噬细胞集落刺激因子(GM-CSF)是一种调节白细胞生长的细胞因子。GM-CSF刺激干细胞产生粒细胞(中性粒细胞、嗜酸性粒细胞和嗜碱性粒细胞)及单核细胞。此外,它对造血和非造血谱系细胞也具有活性。GM-CSF用作药物来刺激白细胞的产生,从而预防化疗后引起的中性粒细胞减少症。近来,人们正在临床试验评估GM-CSF作为一种潜在的疫苗佐剂对HIV感染患者的疗效。相关研究报道一些癌症(包括肺癌和急性髓细胞白血病)患者的血清中具有较高水平的GM-CSF,同样在支气管哮喘患者外周血和鳞屑提取物中也具有较高水平的GM-CSF。

人 Human CSF2 分子靶点信息

  • 分子名:CSF2, colony stimulating factor 2
  • 别称:
    • colony stimulating factor 2 (granulocyte-macrophage)
    • colony-stimulating factor
    • CSF
    • GMCSF
    • granulocyte macrophage-colony stimulating factor
    • granulocyte-macrophage colony stimulating factor
    • granulocyte-macrophage colony-stimulating factor
    • MGC131935
    • MGC138897
    • molgramostim
    • molgramostin
    • sargramostim
  • 基因序列:NCBI_Gene: 1437
  • 蛋白序列:UniProtKB: P04141

人 Human CSF2靶点分子功能(预测)

Enables cytokine activity. Involved in several processes, including cellular response to granulocyte macrophage colony-stimulating factor stimulus; myeloid leukocyte differentiation; and positive regulation of macromolecule metabolic process. Located in extracellular space and intracellular membrane-bounded organelle. Implicated in acute myeloid leukemia; juvenile myelomonocytic leukemia; neutropenia; pulmonary alveolar proteinosis; and visceral leishmaniasis. Biomarker of COVID-19; asthma; chronic obstructive pulmonary disease; and temporal arteritis.

操作步骤


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引用文献

文章目录[隐藏]


  1. Tumor associated neutrophils promote the metastasis of pancreatic ductal adenocarcinoma 

  2. Human natural killer cells for targeting delivery of gold nanostars and bimodal imaging directed photothermal/photodynamic therapy and immunotherapy 

  3. Decidua-derived granulocyte macrophage colony-stimulating factor induces polymorphonuclear myeloid-derived suppressor cells from circulating CD15+ neutrophils 

  4. Nanobody-based CAR T cells targeting intracellular tumor antigens 

  5. Knockdown of Cadherin 26 Prevents the Inflammatory Responses of Allergic Rhinitis 

  6. Tumor-Infiltrating PD-L1+ Neutrophils Induced by GM-CSF Suppress T Cell Function in Laryngeal Squamous Cell Carcinoma and Predict Unfavorable Prognosis 

  7. Chimeric anti-GPC3 sFv-CD3ε receptor-modified T cells with IL7 co-expression for the treatment of solid tumors 

  8. An Oncolytic Adenovirus Encoding Decorin and Granulocyte Macrophage Colony Stimulating Factor Inhibits Tumor Growth in a Colorectal Tumor Model by Targeting Pro-Tumorigenic Signals and via Immune Activation 

  9. GM-CSF-miRNA-Jak2/Stat3 Signaling Mediates Chemotherapy-Induced Cancer Cell Stemness in Gastric Cancer. 

  10. Impaired myeloid-derived suppressor cells are associated with recurrent implantation failure: A case-control study 

  11. IDH2/R140Q mutation confers cytokine-independent proliferation of TF-1 cells by activating constitutive STAT3/5 phosphorylation 

  12. CSF2 upregulates CXCL3 expression in adipocytes to promote metastasis of breast cancer via the FAK signaling pathway 

  13. 细胞培养上清

    Human natural killer cells for targeting delivery of gold nanostars and bimodal imaging directed photothermal/photodynamic therapy and immunotherapy 

  14. A Vascular Disrupting Agent Overcomes Tumor Multidrug Resistance by Skewing Macrophage Polarity toward the M1 Phenotype 

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