EK169S

Human G-CSF Standard (人粒细胞集落刺激因子标准品)

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¥580.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:CSF3, G-CSF
  • 种属:人 (Human)
  • 试剂盒:EK169
  • 保存:短期4℃保存,长期-20℃保存
  • 运输条件:4℃蓝冰运输

在售SKU:70-EK169S


描述

本产品只包含标准品试剂,如需购买试剂盒请点击下图

商品名

Human G-CSF Standard (人粒细胞集落刺激因子标准品)

组分

人 G-CSF标准品

检测方法

双抗夹心法

样本类型

血清,血浆,细胞培养上清及其他生物学样本

板式

保存

短期4℃,长期-20℃保存

运输条件

4℃蓝冰运输

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人G-CSF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的G-CSF与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中G-CSF的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human G-CSF ELISA Kit (人粒细胞集落刺激因子ELISA 试剂盒) - 标准曲线
标准曲线

分子信息

概述人 CSF3, G-CSF 靶点信息

CSF3 分子靶点信息概述

  • 分子名:CSF3, colony stimulating factor 3
  • 基因家族:Receptor ligands
  • 别名:MGC45931
  • 曾用名:GCSF; G-CSF; C17orf33
  • 全称:granulocyte colony stimulating factor; pluripoietin; filgrastim; lenograstim; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte)

CSF3 分子靶点综述

粒细胞集落刺激因子(G-CSF)是一种糖蛋白,可刺激骨髓产生粒细胞和干细胞,并把它们释放到血液中。从功能上来说,它是一种细胞因子和激素,是一种集落刺激因子,由内皮细胞、巨噬细胞和许多其它免疫细胞产生。G-CSF刺激中性粒细胞前体和成熟中性粒细胞的生存、增殖、分化及功能发挥。在肿瘤学和血液学上,重组G-CSF用于加速癌症患者化疗后引起的嗜中性白血球减少症的恢复。在白细胞去除前,G-CSF也可用来增加献血者血液中的造血干细胞数量,用于造血干细胞移植。

人 Human CSF3 分子靶点信息

  • 分子名:CSF3, colony stimulating factor 3
  • 别称:
    • C17orf33
    • chromosome 17 open reading frame 33
    • colony stimulating factor 3 (granulocyte)
    • CSF3OS
    • filgrastim
    • G-CSF
    • GCSF
    • granulocyte colony-stimulating factor
    • granulocyte-colony stimulating factor
    • lenograstim
    • MGC45931
    • pluripoietin
  • 基因序列:NCBI_Gene: 1440
  • 蛋白序列:UniProtKB: P09919

人 Human CSF3靶点分子功能(预测)

Enables cytokine activity and enzyme binding activity. Involved in several processes, including positive regulation of cytoskeleton organization; positive regulation of intracellular signal transduction; and positive regulation of protein phosphorylation. Predicted to be located in endocytic vesicle lumen; extracellular region; and lysosomal lumen. Predicted to be active in extracellular space. Implicated in several diseases, including allergic cutaneous vasculitis; artery disease (multiple); ischemia (multiple); leukemia (multiple); and lung disease (multiple). Biomarker of COVID-19; bacterial pneumonia; and myeloid leukemia.

操作步骤

ELISA操作常见问题

查看更多ELISA操作相关问题

引用文献


  1. Tumor associated neutrophils promote the metastasis of pancreatic ductal adenocarcinoma 

  2. Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 

  3. G-CSF associates with neurogenesis and predicts prognosis and sensitivity to chemotherapy in pancreatic ductal adenocarcinoma 

  4. Cancer-associated adipocyte-derived G-CSF promotes breast cancer malignancy via Stat3 signaling 

  5. Tumor-Infiltrating PD-L1+ Neutrophils Induced by GM-CSF Suppress T Cell Function in Laryngeal Squamous Cell Carcinoma and Predict Unfavorable Prognosis 

  6. A gain-of-function variation in PLCG1 causes a new immune dysregulation disease 

  7. 腹腔上清液

    Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 

  8. G-Csf Associates with Neurogenesis and Predicts Prognosis and Sensitivity to Chemotherapy in Pancreatic Ductal Adenocarcinoma 

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