EK193

Human EGF ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:EGF, epidermal growth factor
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:血清血浆:20 μL;细胞培养上清:100 μL
  • 灵敏度:0.41 pg/mL
  • 检测范围:7.81 pg/mL - 500 pg/mL
  • 回收率:71% - 106%

在售SKU:70-EK193-48, 70-EK193-96


描述

商品名

Human EGF ELISA Kit (人表皮生长因子体 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:1.1% - 3.7%;板间变异系数:3.2% - 6.9%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

血清血浆:20 μL;细胞培养上清:100 μL

灵敏度

0.41 pg/mL

检测范围

7.81 pg/mL - 500 pg/mL

回收率

71% - 106%

平均回收率

0.96

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗EGF单克隆抗体的96孔聚苯乙烯酶标板
  • 人EGF冻干标准品
  • EGF检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人EGF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的EGF与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中EGF的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human EGF ELISA Kit (人表皮生长因子体 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 EGF, epidermal growth factor 靶点信息

EGF 分子靶点信息概述

  • 分子名:EGF, epidermal growth factor
  • 全称:Pro-epidermal growth factor; epidermal growth factor (beta-urogastrone)

EGF 分子靶点综述

表皮生长因子(EGF)是一种通过与其受体EGFR结合,刺激细胞生长、增殖和分化的生长因子。EGF是小分子量多肽,最迟从小鼠下颌下腺纯化得到,随后在很多人的组织中均有发现,包括下颌下腺和腮腺。由饮食中的无机碘调控的唾液EGF在维持奥罗食管和胃组织健全方面具有重要的生理作用。唾液EGF的生理作用包括治愈口腔和胃溃疡、抑制胃酸分泌、刺激DNA合成以及保护粘膜免受腔内有害因素(如胃酸、胆汁酸、胃蛋白酶和胰蛋白酶)和物理、化学和细菌因素破坏。在某些通常与受体的突变和功能异常(如不依赖于EGF水平或与EGF结合的组成性受体信号传导)有关的癌症中,可观察到EGF受体活性增强。

人 Human EGF 分子靶点信息

  • 分子名:EGF, epidermal growth factor
  • 别称:
    • beta-urogastrone
    • HOMG4
    • pro-epidermal growth factor
    • URG
  • 基因序列:NCBI_Gene: 1950
  • 蛋白序列:UniProtKB: P01133

人 Human EGF靶点分子功能(预测)

Enables growth factor activity and guanyl-nucleotide exchange factor activity. Involved in several processes, including ERK1 and ERK2 cascade; positive regulation of macromolecule metabolic process; and positive regulation of signal transduction. Acts upstream of or within positive regulation of cell population proliferation. Located in extracellular exosome. Implicated in several diseases, including Zollinger-Ellison syndrome; hepatobiliary system cancer (multiple); high grade glioma (multiple); lung non-small cell carcinoma (multiple); and primary hypomagnesemia (multiple). Biomarker of several diseases, including acute kidney failure; lupus nephritis; neurodegenerative disease (multiple); pancreatic cancer (multiple); and pancreatitis.

操作步骤

ELISA操作常见问题

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引用文献

文章目录[隐藏]


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  2. Human Umbilical Cord-Derived Mesenchymal Stem Cells Ameliorate Skin Aging of Nude Mice Through Autophagy-Mediated Anti-Senescent Mechanism 

  3. Expression of miR-182 in patients with fracture of tibial plateau and its regulative effects on the fracture healing 

  4. FOXA1 promotes prostate cancer angiogenesis by inducing multiple pro-angiogenic factors expression 

  5. Autocrine TGF-alpha is associated with Benzo(a)pyrene-induced mucus production and MUC5AC expression during allergic asthma 

  6. Growth factors-based platelet lysate rejuvenates skin against ageing through NF-κB signalling pathway: In vitro and in vivo mechanistic and clinical studies 

  7. Transverse Tibial Bone Transport Enhances Distraction Osteogenesis and Vascularization in the Treatment of Diabetic Foot 

  8. Utilizing 3D bioprinted platelet-rich fibrin-based materials to promote the regeneration of oral soft tissue 

  9. Advanced-platelet-rich fibrin extract promotes adipogenic and osteogenic differentiation of human adipose-derived stem cells in a dose-dependent manner in vitro 

  10. Growth factors-based beneficial effects of platelet lysate on umbilical cord-derived stem cells and their synergistic use in osteoarthritis treatment 

  11. Transcription Factor SOX18 Promotes Clear Cell Renal Cell Carcinoma Progression and Alleviates Cabozantinib-Mediated Inhibitory Effects 

  12. KLF5/MDM2 Axis Modulates Oxidative Stress and Epithelial-Mesenchymal Transition in Human Lens Epithelial Cells: The Role In Diabetic Cataract 

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