Human EGF ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:EGF, epidermal growth factor
- 种属:人 (Human)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:血清血浆:20 μL;细胞培养上清:100 μL
- 灵敏度:0.41 pg/mL
- 检测范围:7.81 pg/mL - 500 pg/mL
- 回收率:71% - 106%
在售SKU:70-EK193-48, 70-EK193-96
描述
商品名 |
Human EGF ELISA Kit (人表皮生长因子体 ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:1.1% - 3.7%;板间变异系数:3.2% - 6.9% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
血清血浆:20 μL;细胞培养上清:100 μL |
灵敏度 |
0.41 pg/mL |
检测范围 |
7.81 pg/mL - 500 pg/mL |
回收率 |
71% - 106% |
平均回收率 |
0.96 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人EGF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的EGF与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中EGF的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
EGF 分子靶点信息概述
- 分子名:EGF, epidermal growth factor
- 全称:Pro-epidermal growth factor; epidermal growth factor (beta-urogastrone)
EGF 分子靶点综述
表皮生长因子(EGF)是一种通过与其受体EGFR结合,刺激细胞生长、增殖和分化的生长因子。EGF是小分子量多肽,最迟从小鼠下颌下腺纯化得到,随后在很多人的组织中均有发现,包括下颌下腺和腮腺。由饮食中的无机碘调控的唾液EGF在维持奥罗食管和胃组织健全方面具有重要的生理作用。唾液EGF的生理作用包括治愈口腔和胃溃疡、抑制胃酸分泌、刺激DNA合成以及保护粘膜免受腔内有害因素(如胃酸、胆汁酸、胃蛋白酶和胰蛋白酶)和物理、化学和细菌因素破坏。在某些通常与受体的突变和功能异常(如不依赖于EGF水平或与EGF结合的组成性受体信号传导)有关的癌症中,可观察到EGF受体活性增强。
人 Human EGF 分子靶点信息
- 分子名:EGF, epidermal growth factor
- 别称:
- beta-urogastrone
- HOMG4
- pro-epidermal growth factor
- URG
- 基因序列:NCBI_Gene: 1950
- 蛋白序列:UniProtKB: P01133
人 Human EGF靶点分子功能(预测)
Enables growth factor activity and guanyl-nucleotide exchange factor activity. Involved in several processes, including ERK1 and ERK2 cascade; positive regulation of macromolecule metabolic process; and positive regulation of signal transduction. Acts upstream of or within positive regulation of cell population proliferation. Located in extracellular exosome. Implicated in several diseases, including Zollinger-Ellison syndrome; hepatobiliary system cancer (multiple); high grade glioma (multiple); lung non-small cell carcinoma (multiple); and primary hypomagnesemia (multiple). Biomarker of several diseases, including acute kidney failure; lupus nephritis; neurodegenerative disease (multiple); pancreatic cancer (multiple); and pancreatitis.
操作步骤
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引用文献
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- Autocrine TGF-alpha is associated with Benzo(a)pyrene-induced mucus production and MUC5AC expression during allergic asthma 
- Growth factors-based platelet lysate rejuvenates skin against ageing through NF-κB signalling pathway: In vitro and in vivo mechanistic and clinical studies 
- Transverse Tibial Bone Transport Enhances Distraction Osteogenesis and Vascularization in the Treatment of Diabetic Foot 
- Utilizing 3D bioprinted platelet-rich fibrin-based materials to promote the regeneration of oral soft tissue 
- Advanced-platelet-rich fibrin extract promotes adipogenic and osteogenic differentiation of human adipose-derived stem cells in a dose-dependent manner in vitro 
- Growth factors-based beneficial effects of platelet lysate on umbilical cord-derived stem cells and their synergistic use in osteoarthritis treatment 
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Tumor associated neutrophils promote the metastasis of pancreatic ductal adenocarcinoma 
Human Umbilical Cord-Derived Mesenchymal Stem Cells Ameliorate Skin Aging of Nude Mice Through Autophagy-Mediated Anti-Senescent Mechanism 
Expression of miR-182 in patients with fracture of tibial plateau and its regulative effects on the fracture healing 
FOXA1 promotes prostate cancer angiogenesis by inducing multiple pro-angiogenic factors expression 
Autocrine TGF-alpha is associated with Benzo(a)pyrene-induced mucus production and MUC5AC expression during allergic asthma 
Growth factors-based platelet lysate rejuvenates skin against ageing through NF-κB signalling pathway: In vitro and in vivo mechanistic and clinical studies 
Transverse Tibial Bone Transport Enhances Distraction Osteogenesis and Vascularization in the Treatment of Diabetic Foot 
Utilizing 3D bioprinted platelet-rich fibrin-based materials to promote the regeneration of oral soft tissue 
Advanced-platelet-rich fibrin extract promotes adipogenic and osteogenic differentiation of human adipose-derived stem cells in a dose-dependent manner in vitro 
Growth factors-based beneficial effects of platelet lysate on umbilical cord-derived stem cells and their synergistic use in osteoarthritis treatment 
Transcription Factor SOX18 Promotes Clear Cell Renal Cell Carcinoma Progression and Alleviates Cabozantinib-Mediated Inhibitory Effects 
KLF5/MDM2 Axis Modulates Oxidative Stress and Epithelial-Mesenchymal Transition in Human Lens Epithelial Cells: The Role In Diabetic Cataract 
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