Human Cystatin C ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:CST3, cystatin C
- 种属:人 (Human)
- 样本类型:血清,血浆,细胞上清
- 检测样本体积:100 μL
- 灵敏度:10.57 pg/mL
- 检测范围:31.25 pg/mL - 2000 pg/mL
- 回收率:85% - 115%
在售SKU:70-EK1114-48, 70-EK1114-96
描述
商品名 |
Human Cystatin C ELISA Kit (人半胱氨酸蛋白酶抑制剂C ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:3.3% - 7.9%;板间变异系数:3.1% - 6.2% |
样本类型 |
血清,血浆,细胞上清 |
检测样本体积 |
100 μL |
灵敏度 |
10.57 pg/mL |
检测范围 |
31.25 pg/mL - 2000 pg/mL |
回收率 |
85% - 115% |
平均回收率 |
0.98 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人Cystatin C抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的Cystatin C与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中Cystatin C的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
CST3 分子靶点信息概述
- 分子名:CST3, cystatin C
- 基因家族:Cystatins, type 2
- 全称:cystatin C (amyloid angiopathy and cerebral hemorrhage)
CST3 分子靶点综述
半胱氨酸蛋白酶抑制剂C属于半胱氨酸蛋白酶抑制剂超家族,是溶酶体蛋白酶的有效抑制剂,可能是半胱氨酸蛋白酶最重要的胞外抑制剂之一。它几乎存在于所有组织和体液中,包括血浆、尿液和脑脊液。Cystatin C通过调节半胱氨酸蛋白酶活性参与多种疾病过程。Cystatin C主要作为肾功能的生物标志物。最近,研究显示它在预测新发或恶化的心血管疾病中起作用。另外,它在淀粉样蛋白有关的脑疾病如阿尔茨海默氏病中发挥作用。
人 Human CST3 分子靶点信息
- 分子名:CST3, cystatin C
- 别称:
- ARMD11
- bA218C14.4 (cystatin C)
- cystatin 3
- cystatin C (amyloid angiopathy and cerebral hemorrhage)
- cystatin-3
- cystatin-C
- epididymis secretory protein Li 2
- gamma-trace
- HEL-S-2
- MGC117328
- neuroendocrine basic polypeptide
- post-gamma-globulin
- 基因序列:NCBI_Gene: 1471
- 蛋白序列:UniProtKB: P01034
人 Human CST3靶点分子功能(预测)
Enables several functions, including amyloid-beta binding activity; cysteine-type endopeptidase inhibitor activity; and identical protein binding activity. Involved in several processes, including negative regulation of blood vessel remodeling; negative regulation of catabolic process; and negative regulation of extracellular matrix disassembly. Located in Golgi apparatus; extracellular space; and plasma membrane. Implicated in CST3-related cerebral amyloid angiopathy and age related macular degeneration 11. Biomarker of several diseases, including artery disease (multiple); autoimmune disease of the nervous system (multiple); brain ischemia; obesity; and type 2 diabetes mellitus.
操作步骤
文章目录[隐藏]
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引用文献
文章目录[隐藏]
- Effects of continuous negative pressure suction combined with autologous platelet-rich gel on the levels of CRP, IL-6, wound healing and length of stay in clients with diabetic foot 
- Clinical value of combined plasma brain natriuretic peptide and serum cystatin C measurement on the prediction of heart failure in patients after acute myocardial infarction 
- Microcystin-LR-Induced Interaction between M2 Tumor-Associated Macrophage and Colorectal Cancer Cell Promotes Colorectal Cancer Cell Migration through Regulating the Expression of TGF-β1 and CST3 
Effects of continuous negative pressure suction combined with autologous platelet-rich gel on the levels of CRP, IL-6, wound healing and length of stay in clients with diabetic foot 
Clinical value of combined plasma brain natriuretic peptide and serum cystatin C measurement on the prediction of heart failure in patients after acute myocardial infarction 
Microcystin-LR-Induced Interaction between M2 Tumor-Associated Macrophage and Colorectal Cancer Cell Promotes Colorectal Cancer Cell Migration through Regulating the Expression of TGF-β1 and CST3 
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