Human CXCL5/ENA-78 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:CXCL5, ENA-78
- 种属:人 (Human)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:10 μL
- 灵敏度:13.45 pg/mL
- 检测范围:31.25 pg/mL - 2000 pg/mL
- 回收率:82% - 125%
在售SKU:70-EK1219-48, 70-EK1219-96
描述
商品名 |
Human CXCL5/ENA-78 ELISA Kit (人趋化因子CXC配体5/上皮来源的中性粒细胞激活肽78 ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:4.9% - 6.4%;板间变异系数:2.8% - 3.9% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
10 μL |
灵敏度 |
13.45 pg/mL |
检测范围 |
31.25 pg/mL - 2000 pg/mL |
回收率 |
82% - 125% |
平均回收率 |
1.01 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人 CXCL5 抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的 CXCL5 与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后,加入显色底物 TMB,避光显色。颜色反应的深浅与样本中 CXCL5 的浓度成正比。加入终止液终止反应,在 450nm 波长(参考波长 570 - 630 nm)测定吸光度值。
分子信息
CXCL5 分子靶点信息概述
- 分子名:CXCL5, C-X-C motif chemokine ligand 5
- 基因家族:Chemokine ligands
- 别名:ENA-78
- 曾用名:SCYB5
- 全称:small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78); chemokine (C-X-C motif) ligand 5
CXCL5 分子靶点综述
C-X-C基序趋化因子5(CXCL5),又名上皮来源的中性粒细胞激活肽78(ENA-78),是CXC趋化因子家族中的小分子量细胞因子。炎性细胞因子IL-1或TNF-α刺激细胞后可产生CXCL5,嗜酸性细胞也可表达CXCL5,而II型干扰素IFN-γ可抑制其表达。CXCL5通过与细胞表面趋化因子受体CXCR2相互作用来发挥其作用效果,可刺激具有血管生成特性的中性粒细胞的趋化性,并参与结缔组织重塑。CXCL5同样具有调节中性粒细胞稳态的作用。CXCL5在动脉粥样硬化(一种慢性炎症状态)中表达较高,但与中性粒细胞的浸润无关。相反,CXCL5可直接控制巨噬细胞的泡沫细胞形成,从而对动脉粥样硬化起保护作用。
人 Human CXCL5 分子靶点信息
- 分子名:CXCL5, C-X-C motif chemokine ligand 5
- 别称:
- C-X-C motif chemokine 5
- chemokine (C-X-C motif) ligand 5
- ENA-78
- ENA-78(1-78)
- epithelial-derived neutrophil activating protein 78
- epithelial-derived neutrophil-activating peptide 78
- epithelial-derived neutrophil-activating protein 78
- neutrophil-activating peptide ENA-78
- neutrophil-activating protein 78
- SCYB5
- small inducible cytokine B5
- small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78)
- small-inducible cytokine B5
- 基因序列:NCBI_Gene: 6374
- 蛋白序列:UniProtKB: P42830
人 Human CXCL5靶点分子功能(预测)
Enables identical protein binding activity. Predicted to be involved in several processes, including cellular response to lipopolysaccharide; chemokine-mediated signaling pathway; and neutrophil chemotaxis. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Implicated in idiopathic pulmonary fibrosis. Biomarker of several diseases, including common cold; diffuse scleroderma; hyperhomocysteinemia; lung disease (multiple); and sinusitis.
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
- 血清OR血浆,哪个是ELISA的菜 
- ELISA通关必备丨数据篇丨标准曲线不佳 
- ELISA通关必备丨操作篇丨常见问题及解决方案 
- ELISA通关必备丨操作篇丨溶解与稀释标准品 
- ELISA通关必备丨样本篇丨不常见样本 
- ELISA通关必备丨样本篇丨常见样本丨细胞 
- ELISA通关必备丨如何选择试剂盒 
- ELISA通关必备丨基础知识 
- 真?假?ELISA试剂盒选择要小心 
- ELISA常见类型一 | 双抗夹心法,你要的都在这里! 
- ELISA常见类型二 | 竞争法,五分钟搞定! 
- 叮!联科向您投递了个ELISA实验操作干货包,请查收~ 
- 【视频】ELISA实验操作步骤演示视频教程 
- ELISA 组织样本的处理—大鼠组织 
- 【视频】ELISA实验原理与常见问题分析 
- 查看更多ELISA操作相关问题
ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
文章目录[隐藏]
- Periodontal inflammation recruits distant metastatic breast cancer cells by increasing myeloid-derived suppressor cells 
- HPV11E7 inhibits IMQ-induced chemokine and colony-stimulating factor production in keratinocytes 
- The kinase activity of integrin-linked kinase regulates cellular senescence in gastric cancer 
- An inflammatory checkpoint generated by IL1RN splicing offers therapeutic opportunity for KRAS mutant intrahepatic cholangiocarcinoma 
- Constraint-induced movement therapy alleviates motor impairment by inhibiting the accumulation of neutrophil extracellular traps in ischemic cortex 
- Dedifferentiation-associated inflammatory factors of long-term expanded human hepatocytes exacerbate their elimination by macrophages during liver engraftment 
Periodontal inflammation recruits distant metastatic breast cancer cells by increasing myeloid-derived suppressor cells 
HPV11E7 inhibits IMQ-induced chemokine and colony-stimulating factor production in keratinocytes 
The kinase activity of integrin-linked kinase regulates cellular senescence in gastric cancer 
An inflammatory checkpoint generated by IL1RN splicing offers therapeutic opportunity for KRAS mutant intrahepatic cholangiocarcinoma 
Constraint-induced movement therapy alleviates motor impairment by inhibiting the accumulation of neutrophil extracellular traps in ischemic cortex 
- 细胞培养上清
Dedifferentiation-associated inflammatory factors of long-term expanded human hepatocytes exacerbate their elimination by macrophages during liver engraftment 
评价
目前还没有评价