Human CXCL3/GRO-γ ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:CXCL3, MIP-2b, GRO3, GROg
- 种属:人 (Human)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:100 μL(稀释液)
- 灵敏度:0.24 pg/mL
- 检测范围:1.56 pg/mL - 100 pg/mL
- 回收率:86% - 105%
在售SKU:70-EK1265-48, 70-EK1265-96
描述
商品名 |
Human CXCL3/GRO-γ ELISA Kit (人趋化因子 (C-X-C基序) 配体3 (CXCL3/GRO-γ) ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:2.6% - 5.5%;板间变异系数:2.3% - 11.5% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
100 μL(稀释液) |
灵敏度 |
0.24 pg/mL |
检测范围 |
1.56 pg/mL - 100 pg/mL |
回收率 |
86% - 105% |
平均回收率 |
0.95 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人CXCL3抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的CXCL3与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中CXCL3的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
CXCL3 分子靶点信息概述
- 分子名:CXCL3, C-X-C motif chemokine ligand 3
- 基因家族:Chemokine ligands
- 别名:SCYB3; GROg; MIP-2b; CINC-2b
- 曾用名:GRO3
- 全称:GRO3 oncogene; chemokine (C-X-C motif) ligand 3
CXCL3 分子靶点综述
CXCL1、CXCL2和CXCL3,分别称为GRO alpha, GRO beta (MIP-2 alpha) 和GRO gamma (MIP-2 beta),是趋化因子的CXC亚家族成员。成熟的CXCL1/2/3蛋白与B型IL-8受体高度亲和结合,是有效的中性粒细胞吸引剂和活化剂。趋化因子主要作用于白细胞,调节其运输。一般来说,趋化因子α家族成员优先趋化并激活中性粒细胞。重组人生长调节致癌基因γ(GRO-γ)是一种7.9kDa蛋白质,由73个氨基酸组成,包括与CXCR1或CXCR2结合的CXC趋化因子家族共有的“ELR”基序。成熟的人CXCL3 (aa 35-107)与小鼠和大鼠CXCL3的氨基酸序列同源性分别为70%和74%。CXCL3通过与CXCR2受体的相互作用,在单核细胞迁移和粘附中起着关键作用。
人 Human CXCL3 分子靶点信息
- 分子名:CXCL3, C-X-C motif chemokine ligand 3
- 别称:
- C-X-C motif chemokine 3
- chemokine (C-X-C motif) ligand 3
- CINC-2b
- GRO-gamma
- GRO-gamma(1-73)
- GRO3
- GRO3 oncogene
- GROg
- growth-regulated protein gamma
- macrophage inflammatory protein 2-beta
- melanoma growth stimulatory activity gamma
- MGSA gamma
- MIP-2b
- MIP2-beta
- MIP2B
- SCYB3
- 基因序列:NCBI_Gene: 2921
- 蛋白序列:UniProtKB: P19876
人 Human CXCL3靶点分子功能(预测)
Predicted to enable CXCR chemokine receptor binding activity and chemokine activity. Involved in antimicrobial humoral immune response mediated by antimicrobial peptide and killing of cells of other organism. Located in extracellular space.
操作步骤
文章目录[隐藏]
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- 真?假?ELISA试剂盒选择要小心 
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引用文献
文章目录[隐藏]
- Macrophage polarization toward M1 phenotype through NF-κB signaling in patients with Behçet’s disease 
- HPV11E7 inhibits IMQ-induced chemokine and colony-stimulating factor production in keratinocytes 
- Interleukin-26 Expression in Inflammatory Bowel Disease and Its Immunoregulatory Effects on Macrophages 
- Cancer-associated adipocytes promote the invasion and metastasis in breast cancer through LIF/CXCLs positive feedback loop 
- Deciphering m6A methylation in monocyte-mediated cardiac fibrosis and monocyte-hitchhiked erythrocyte microvesicle biohybrid therapy 
- Salmonella spvC gene suppresses macrophage/neutrophil antibacterial defense mediated by gasdermin D 
- The spike protein of SARS-CoV-2 induces inflammation and EMT of lung epithelial cells and fibroblasts through the upregulation of GADD45A 
- Self-recruited neutrophils trigger over-activated innate immune response and phenotypic change of cardiomyocytes in fulminant viral myocarditis 
- Single-cell transcriptomes reveal a molecular link between diabetic kidney and retinal lesions 
- An inflammatory checkpoint generated by IL1RN splicing offers therapeutic opportunity for KRAS mutant intrahepatic cholangiocarcinoma 
- CSF2 upregulates CXCL3 expression in adipocytes to promote metastasis of breast cancer via the FAK signaling pathway 
- Dedifferentiation-associated inflammatory factors of long-term expanded human hepatocytes exacerbate their elimination by macrophages during liver engraftment 
Macrophage polarization toward M1 phenotype through NF-κB signaling in patients with Behçet’s disease 
HPV11E7 inhibits IMQ-induced chemokine and colony-stimulating factor production in keratinocytes 
Interleukin-26 Expression in Inflammatory Bowel Disease and Its Immunoregulatory Effects on Macrophages 
Cancer-associated adipocytes promote the invasion and metastasis in breast cancer through LIF/CXCLs positive feedback loop 
Deciphering m6A methylation in monocyte-mediated cardiac fibrosis and monocyte-hitchhiked erythrocyte microvesicle biohybrid therapy 
Salmonella spvC gene suppresses macrophage/neutrophil antibacterial defense mediated by gasdermin D 
The spike protein of SARS-CoV-2 induces inflammation and EMT of lung epithelial cells and fibroblasts through the upregulation of GADD45A 
Self-recruited neutrophils trigger over-activated innate immune response and phenotypic change of cardiomyocytes in fulminant viral myocarditis 
Single-cell transcriptomes reveal a molecular link between diabetic kidney and retinal lesions 
An inflammatory checkpoint generated by IL1RN splicing offers therapeutic opportunity for KRAS mutant intrahepatic cholangiocarcinoma 
CSF2 upregulates CXCL3 expression in adipocytes to promote metastasis of breast cancer via the FAK signaling pathway 
- 细胞培养上清
Dedifferentiation-associated inflammatory factors of long-term expanded human hepatocytes exacerbate their elimination by macrophages during liver engraftment 
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