EK1119

Human CXCL12/SDF-1 ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：CXCL12, SDF1, PBSF
  • 种属：人 (Human)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：20 μL
  • 灵敏度：18.13 pg/mL
  • 检测范围：62.5 pg/mL - 4000 pg/mL
  • 回收率：89% - 110%

在售SKU:70-EK1119-48, 70-EK1119-96

描述

商品名

Human CXCL12/SDF-1 ELISA Kit (人趋化因子CXC配体12 ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：2.4% - 2.6%；板间变异系数：3.0% - 4.5%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

20 μL
灵敏度

18.13 pg/mL
检测范围

62.5 pg/mL - 4000 pg/mL
回收率

89% - 110%
平均回收率

1.02
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗CXCL12/SDF-1单克隆抗体的96孔聚苯乙烯酶标板
  • 人CXCL12/SDF-1冻干标准品
  • CXCL12/SDF-1检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人CXCL12抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的CXCL12与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中CXCL12的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human CXCL12/SDF-1 ELISA Kit (人趋化因子CXC配体12 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 CXCL12, SDF1, PBSF 靶点信息

CXCL12 分子靶点信息概述

  • 分子名：CXCL12, C-X-C motif chemokine ligand 12
  • 基因家族：Chemokine ligands
  • 别名：SCYB12; SDF-1a; SDF-1b; PBSF; TLSF-a; TLSF-b; TPAR1
  • 曾用名：SDF1A; SDF1B; SDF1
  • 全称：stromal cell-derived factor 1; chemokine (C-X-C motif) ligand 12

CXCL12 分子靶点综述

趋化因子CXC配体12(CXCL12)也称为基质细胞衍生因子1(SDF-1)，是一种可活化白细胞的趋化因子蛋白，通常由促炎性刺激剂诱导产生，如LPS、TNF或IL-1。CXCL12对淋巴细胞具有强烈的趋化作用。在胚胎发生期，它可促使造血细胞从胎肝向骨髓迁移和大血管的形成。在成年人中，CXCL12在血管生成中具有重要作用，它通过CXCR4依赖的机制招募骨髓中的内皮祖细胞(EPCs)。CXCL12对肿瘤转移也有作用，表达CXCR4受体的癌细胞可被吸引至靶组织。然而在乳腺癌中，CXCL12表达升高则提示远端转移的风险降低。

人 Human CXCL12 分子靶点信息

  • 分子名：CXCL12, C-X-C motif chemokine ligand 12
  • 别称：
    • chemokine (C-X-C motif) ligand 12
    • intercrine reduced in hepatomas
    • IRH
    • PBSF
    • pre-B cell growth-stimulating factor
    • SCYB12
    • SDF-1a
    • SDF-1b
    • SDF1
    • SDF1A
    • SDF1B
    • stromal cell-derived factor 1
    • TLSF
    • TLSF-a
    • TLSF-b
    • TPAR1
  • 基因序列：NCBI_Gene: 6387
  • 蛋白序列：UniProtKB: P48061

人 Human CXCL12靶点分子功能（预测）

Enables CXCR chemokine receptor binding activity; chemokine activity; and integrin binding activity. Involved in several processes, including integrin activation; negative regulation of apoptotic process; and regulation of leukocyte migration. Acts upstream of or within positive regulation of T cell migration and positive regulation of cell adhesion. Located in extracellular exosome. Part of collagen-containing extracellular matrix. Implicated in breast carcinoma; diabetic angiopathy; human immunodeficiency virus infectious disease; and type 1 diabetes mellitus. Biomarker of diabetic retinopathy; insulinoma; periodontitis; and type 2 diabetes mellitus.

操作步骤


下载本产品说明书

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. Curcumin suppresses the proliferation and tumorigenicity of Cal27 by modulating cancer-associated fibroblasts of TSCC 

  2. Periodontal inflammation recruits distant metastatic breast cancer cells by increasing myeloid-derived suppressor cells 

  3. Insulin Exacerbates Inflammation in Fibroblast-Like Synoviocytes 

  4. Functional decellularized fibrocartilaginous matrix graft for rotator cuff enthesis regeneration: A novel technique to avoid in-vitro loading of cells 

  5. SDF-1α peptide tethered polyester facilitates tissue repair by endogenous cell mobilization and recruitment 

  6. CREB activation affects mesenchymal stem cell migration and differentiation in periodontal tissues due to orthodontic force 

  7. Exogenous stromal derived factor-1 releasing silk scaffold combined with intra-articular injection of progenitor cells promotes bone-ligament-bone regeneration 

  8. Sustained release of collagen-affinity SDF-1α from book-shaped acellular fibrocartilage scaffold enhanced bone-tendon healing in a rabbit model 

  9. Comprehensive Analysis of Immune Responses to Neoadjuvant Immunotherapy in Resectable Non-small Cell Lung Cancer 

  10. Engineered extracellular vesicles for targeted reprogramming of cancer-associated fibroblasts to potentiate therapy of pancreatic cancer 

  11. Intratumoral CXCR4hi neutrophils display ferroptotic and immunosuppressive signatures in hepatoblastoma 

  12. Dysregulated CXCL12 expression in osteoblasts promotes B-lymphocytes preferentially homing to the bone marrow in MRL/lpr mice 

  13. Optimized Allogenic Decellularized Meniscal Scaffold Modified by Collagen Affinity Stromal Cell–Derived Factor SDF1α for Meniscal Regeneration: A 6- and 12-Week Animal Study in a Rabbit Model 

  14. Bruceine D suppresses CAF-promoted TNBC metastasis under TNF-α stimulation by inhibiting Notch1-Jagged1/NF-κB(p65) signaling 

  15. CDYL knockdown reduces glioma development through an antitumor immune response in the tumor microenvironment 

  16. Cascade Regulation of the Proliferation, Recruitment, and Differentiation of Stem Cells to Prevent Aseptic Loosening by GNPs/ECPP Particles Responding to Macrophages: In Vitro and In Vivo 

  17. Exogenous Stromal Derived Factor-1 Releasing Silk Scaffold Combined with Intra-Articular Injection of Progenitor Cells Promotes Bone-Ligament-Bone Regeneration 

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