Human CXCL-10/IP-10 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
- 分子靶点:CXCL10, INP10, IP-10
- 种属:人 (Human)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:50 μL
- 灵敏度:3.50 pg/mL
- 检测范围:31.25 pg/mL - 2000 pg/mL
- 回收率:78% - 114%
在售SKU:70-EK168-48, 70-EK168-96
描述
商品名 |
Human CXCL10/IP-10 ELISA Kit (人趋化因子CXC配体10/γ-干扰素诱导蛋白10 ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:3.0% - 5.0%;板间变异系数:3.8% - 6.1% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
50 μL |
灵敏度 |
3.50 pg/mL |
检测范围 |
31.25 pg/mL - 2000 pg/mL |
回收率 |
78% - 114% |
平均回收率 |
0.93 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IP-10抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IP-10与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IP-10的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
CXCL10 分子靶点信息概述
- 分子名:CXCL10, C-X-C motif chemokine ligand 10
- 基因家族:Chemokine ligands
- 别名:IFI10; IP-10; crg-2; mob-1; C7; gIP-10
- 曾用名:INP10; SCYB10
- 全称:small inducible cytokine subfamily B (Cys-X-Cys), member 10; chemokine (C-X-C motif) ligand 10
CXCL10 分子靶点综述
趋化因子配体10(CXCL10)又称为γ-干扰素诱导蛋白10(IP-10)或微小诱导细胞因子B10,是属于CXC趋化因子家族的小分子量细胞因子。多种类型的细胞对IFN-γ产生应答后可分泌IP-10,包括单核细胞、内皮细胞和成纤维细胞等。IP-10具有多种功能,如对单核/巨噬细胞、T细胞、NK细胞和树突细胞的趋化作用,促进T细胞黏附于内皮细胞,抗肿瘤活性和抑制骨髓集落形成和血管生成。
人 Human CXCL10 分子靶点信息
- 分子名:CXCL10, C-X-C motif chemokine ligand 10
- 别称:
- 10 kDa interferon gamma-induced protein
- C-X-C motif chemokine 10
- C7
- chemokine (C-X-C motif) ligand 10
- crg-2
- gamma IP10
- gamma-IP10
- gIP-10
- IFI10
- INP10
- interferon-inducible cytokine IP-10
- IP-10
- mob-1
- protein 10 from interferon (gamma)-induced cell line
- SCYB10
- small inducible cytokine subfamily B (Cys-X-Cys), member 10
- small-inducible cytokine B10
- 基因序列:NCBI_Gene: 3627
- 蛋白序列:UniProtKB: P02778
人 Human CXCL10靶点分子功能(预测)
Enables chemoattractant activity; chemokine receptor binding activity; and heparin binding activity. Involved in several processes, including positive regulation of release of sequestered calcium ion into cytosol; regulation of leukocyte chemotaxis; and response to virus. Acts upstream of or within cellular response to lipopolysaccharide and positive regulation of T cell migration. Located in extracellular region. Implicated in hepatitis B and middle cerebral artery infarction. Biomarker of several diseases, including artery disease (multiple); diabetes mellitus (multiple); fatty liver disease (multiple); liver disease (multiple); and lung disease (multiple).
操作步骤
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引用文献
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- SLC4A4 as a novel biomarker involved in immune system response and lung adenocarcinoma progression 
- LncRNA lnc-SPRR2G-2 contributes to keratinocyte hyperproliferation and inflammation in psoriasis by activating the STAT3 pathway and downregulating KHSRP 
- HIF-1α Mediates Immunosuppression and Chemoresistance in Colorectal Cancer by Inhibiting CXCL9, ?10 and ?11 
- The TOPK inhibitor HI-TOPK-032 enhances CAR T-cell therapy of hepatocellular carcinoma by upregulating memory T cells 
- Role of Chemotaxis of Vδ2 T Cells to the Synovium in the Pathogenesis of Acute Gouty Arthritis 
- LPCAT1 facilitates keratinocyte hyperproliferation and skin inflammation in psoriasis via regulating GLUT3 
- eIF4E plays the role of a pathogenic gene in psoriasis, and the inhibition of eIF4E phosphorylation ameliorates psoriasis-like skin damage 
- Triphenyl phosphate exposure impairs colorectal health by altering host immunity and colorectal microbiota 
- Metabolic Reprogramming via ACOD1 depletion enhances function of human induced pluripotent stem cell-derived CAR-macrophages in solid tumors 
- OTUB1's role in promoting OSCC development by stabilizing RACK1 involves cell proliferation, migration, invasion, and tumor-associated macrophage M1 polarization 
- Polygonatum kingianum saponins delay cellular senescence through SASP down-regulation and prolong the healthy lifespan of Caenorhabditis elegans by activating sir-2.1/autophagy 
- NK cell-derived exosomes enhance the anti-tumor effects against ovarian cancer by delivering cisplatin and reactivating NK cell functions 
SLC4A4 as a novel biomarker involved in immune system response and lung adenocarcinoma progression 
LncRNA lnc-SPRR2G-2 contributes to keratinocyte hyperproliferation and inflammation in psoriasis by activating the STAT3 pathway and downregulating KHSRP 
HIF-1α Mediates Immunosuppression and Chemoresistance in Colorectal Cancer by Inhibiting CXCL9, ?10 and ?11 
The TOPK inhibitor HI-TOPK-032 enhances CAR T-cell therapy of hepatocellular carcinoma by upregulating memory T cells 
Role of Chemotaxis of Vδ2 T Cells to the Synovium in the Pathogenesis of Acute Gouty Arthritis 
LPCAT1 facilitates keratinocyte hyperproliferation and skin inflammation in psoriasis via regulating GLUT3 
eIF4E plays the role of a pathogenic gene in psoriasis, and the inhibition of eIF4E phosphorylation ameliorates psoriasis-like skin damage 
Triphenyl phosphate exposure impairs colorectal health by altering host immunity and colorectal microbiota 
Metabolic Reprogramming via ACOD1 depletion enhances function of human induced pluripotent stem cell-derived CAR-macrophages in solid tumors 
OTUB1's role in promoting OSCC development by stabilizing RACK1 involves cell proliferation, migration, invasion, and tumor-associated macrophage M1 polarization 
Polygonatum kingianum saponins delay cellular senescence through SASP down-regulation and prolong the healthy lifespan of Caenorhabditis elegans by activating sir-2.1/autophagy 
NK cell-derived exosomes enhance the anti-tumor effects against ovarian cancer by delivering cisplatin and reactivating NK cell functions 
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