Human CXCL-10/IP-10 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:CXCL10, INP10, IP-10
- 种属:人 (Human)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:50 μL
- 灵敏度:3.50 pg/mL
- 检测范围:31.25 pg/mL - 2000 pg/mL
- 回收率:78% - 114%
在售SKU:70-EK168-48, 70-EK168-96
描述
商品名 |
Human CXCL10/IP-10 ELISA Kit (人趋化因子CXC配体10/γ-干扰素诱导蛋白10 ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:3.0% - 5.0%;板间变异系数:3.8% - 6.1% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
50 μL |
灵敏度 |
3.50 pg/mL |
检测范围 |
31.25 pg/mL - 2000 pg/mL |
回收率 |
78% - 114% |
平均回收率 |
0.93 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IP-10抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IP-10与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IP-10的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
CXCL10 分子靶点信息概述
- 分子名:CXCL10, C-X-C motif chemokine ligand 10
- 基因家族:Chemokine ligands
- 别名:IFI10; IP-10; crg-2; mob-1; C7; gIP-10
- 曾用名:INP10; SCYB10
- 全称:small inducible cytokine subfamily B (Cys-X-Cys), member 10; chemokine (C-X-C motif) ligand 10
CXCL10 分子靶点综述
趋化因子配体10(CXCL10)又称为γ-干扰素诱导蛋白10(IP-10)或微小诱导细胞因子B10,是属于CXC趋化因子家族的小分子量细胞因子。多种类型的细胞对IFN-γ产生应答后可分泌IP-10,包括单核细胞、内皮细胞和成纤维细胞等。IP-10具有多种功能,如对单核/巨噬细胞、T细胞、NK细胞和树突细胞的趋化作用,促进T细胞黏附于内皮细胞,抗肿瘤活性和抑制骨髓集落形成和血管生成。
人 Human CXCL10 分子靶点信息
- 分子名:CXCL10, C-X-C motif chemokine ligand 10
- 别称:
- 10 kDa interferon gamma-induced protein
- C-X-C motif chemokine 10
- C7
- chemokine (C-X-C motif) ligand 10
- crg-2
- gamma IP10
- gamma-IP10
- gIP-10
- IFI10
- INP10
- interferon-inducible cytokine IP-10
- IP-10
- mob-1
- protein 10 from interferon (gamma)-induced cell line
- SCYB10
- small inducible cytokine subfamily B (Cys-X-Cys), member 10
- small-inducible cytokine B10
- 基因序列:NCBI_Gene: 3627
- 蛋白序列:UniProtKB: P02778
人 Human CXCL10靶点分子功能(预测)
Enables chemoattractant activity; chemokine receptor binding activity; and heparin binding activity. Involved in several processes, including positive regulation of release of sequestered calcium ion into cytosol; regulation of leukocyte chemotaxis; and response to virus. Acts upstream of or within cellular response to lipopolysaccharide and positive regulation of T cell migration. Located in extracellular region. Implicated in hepatitis B and middle cerebral artery infarction. Biomarker of several diseases, including artery disease (multiple); diabetes mellitus (multiple); fatty liver disease (multiple); liver disease (multiple); and lung disease (multiple).
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
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- ELISA通关必备丨基础知识 
- 真?假?ELISA试剂盒选择要小心 
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引用文献
文章目录[隐藏]
- The Expression of CXCL10/CXCR3 and Effect of the Axis on the Function of T Lymphocyte Involved in Oral Lichen Planus 
- Prognostic and predictive values of CXCL10 in colorectal cancer 
- Immune infiltration profiling in gastric cancer and their clinical implications 
- Changes in peripheral blood cytokines in patients with severe fever with thrombocytopenia syndrome 
- Potential biomarkers for diagnosis and assessment of disease activity in systemic lupus erythematosus 
- IgD-activated mast cells induce IgE synthesis in B cells in nasal polyps 
- STING agonist cGAMP enhances anti-tumor activity of CAR-NK cells against pancreatic cancer 
- SLC4A4 as a novel biomarker involved in immune system response and lung adenocarcinoma progression 
- LncRNA lnc-SPRR2G-2 contributes to keratinocyte hyperproliferation and inflammation in psoriasis by activating the STAT3 pathway and downregulating KHSRP 
- HIF-1α Mediates Immunosuppression and Chemoresistance in Colorectal Cancer by Inhibiting CXCL9, ?10 and ?11 
- The TOPK inhibitor HI-TOPK-032 enhances CAR T-cell therapy of hepatocellular carcinoma by upregulating memory T cells 
- Role of Chemotaxis of Vδ2 T Cells to the Synovium in the Pathogenesis of Acute Gouty Arthritis 
- LPCAT1 facilitates keratinocyte hyperproliferation and skin inflammation in psoriasis via regulating GLUT3 
- eIF4E plays the role of a pathogenic gene in psoriasis, and the inhibition of eIF4E phosphorylation ameliorates psoriasis-like skin damage 
- Triphenyl phosphate exposure impairs colorectal health by altering host immunity and colorectal microbiota 
- Metabolic Reprogramming via ACOD1 depletion enhances function of human induced pluripotent stem cell-derived CAR-macrophages in solid tumors 
- OTUB1's role in promoting OSCC development by stabilizing RACK1 involves cell proliferation, migration, invasion, and tumor-associated macrophage M1 polarization 
- Polygonatum kingianum saponins delay cellular senescence through SASP down-regulation and prolong the healthy lifespan of Caenorhabditis elegans by activating sir-2.1/autophagy 
- NK cell-derived exosomes enhance the anti-tumor effects against ovarian cancer by delivering cisplatin and reactivating NK cell functions 
The Expression of CXCL10/CXCR3 and Effect of the Axis on the Function of T Lymphocyte Involved in Oral Lichen Planus 
Prognostic and predictive values of CXCL10 in colorectal cancer 
Immune infiltration profiling in gastric cancer and their clinical implications 
Changes in peripheral blood cytokines in patients with severe fever with thrombocytopenia syndrome 
Potential biomarkers for diagnosis and assessment of disease activity in systemic lupus erythematosus 
IgD-activated mast cells induce IgE synthesis in B cells in nasal polyps 
STING agonist cGAMP enhances anti-tumor activity of CAR-NK cells against pancreatic cancer 
SLC4A4 as a novel biomarker involved in immune system response and lung adenocarcinoma progression 
LncRNA lnc-SPRR2G-2 contributes to keratinocyte hyperproliferation and inflammation in psoriasis by activating the STAT3 pathway and downregulating KHSRP 
HIF-1α Mediates Immunosuppression and Chemoresistance in Colorectal Cancer by Inhibiting CXCL9, ?10 and ?11 
The TOPK inhibitor HI-TOPK-032 enhances CAR T-cell therapy of hepatocellular carcinoma by upregulating memory T cells 
Role of Chemotaxis of Vδ2 T Cells to the Synovium in the Pathogenesis of Acute Gouty Arthritis 
LPCAT1 facilitates keratinocyte hyperproliferation and skin inflammation in psoriasis via regulating GLUT3 
eIF4E plays the role of a pathogenic gene in psoriasis, and the inhibition of eIF4E phosphorylation ameliorates psoriasis-like skin damage 
Triphenyl phosphate exposure impairs colorectal health by altering host immunity and colorectal microbiota 
Metabolic Reprogramming via ACOD1 depletion enhances function of human induced pluripotent stem cell-derived CAR-macrophages in solid tumors 
OTUB1's role in promoting OSCC development by stabilizing RACK1 involves cell proliferation, migration, invasion, and tumor-associated macrophage M1 polarization 
Polygonatum kingianum saponins delay cellular senescence through SASP down-regulation and prolong the healthy lifespan of Caenorhabditis elegans by activating sir-2.1/autophagy 
NK cell-derived exosomes enhance the anti-tumor effects against ovarian cancer by delivering cisplatin and reactivating NK cell functions 
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