EK1129

Human CCL5/RANTES ELISA Kit检测试剂盒(酶联免疫吸附法)

¥1,600.00¥2,650.00

在售SKU:70-EK1129-48, 70-EK1129-96, EK1129

  • 分子靶点:CCL5
  • 种属:
  • 样本类型:血清、血浆、细胞培养上清及其他生物学样本
  • 检测样本体积:血清、血浆样本5μL
  • 灵敏度:0.53pg/mL
  • 检测范围:15.63-1000pg/mL
  • 回收率:84%-117%

ELISA试剂盒详细信息

商品名人趋化因子 CC 配体5/调节活化的正常T细胞表达和分泌的因子酶联免疫检测试剂盒
种属
靶点CCL5
检测方法双抗体夹心法
检测样本类型血清、血浆、细胞培养上清及其他生物学样本
检测样本体积血清、血浆样本5μL
灵敏度0.53pg/mL
线性范围15.63-1000pg/mL
精密度板内变异系数:6.9%-7.8%;板间变异系数:5.5%-8.3%
回收率84%-117%
平均回收率97%
板式96孔板,可拆
保存条件2-8℃保存。已拆开:标准品-20℃保存,其它4℃。
运输条件2-8℃冰袋运输
检测原理本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人CCL5抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的CCL5与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中CCL5的浓度成正比。加入终止液终止反应,在450nm波长(参考波长570 - 630nm)测定吸光度值。

分子信息

概述人 CCL5, RANTES, SISd 靶点信息

CCL5 分子靶点信息概述

  • 分子名:CCL5, C-C motif chemokine ligand 5
  • 基因家族:Chemokine ligands
  • 别名:RANTES; SISd; TCP228; MGC17164
  • 曾用名:D17S136E; SCYA5
  • 全称:T-cell specific protein p288; T-cell specific RANTES protein; SIS-delta; regulated upon activation, normally T-expressed, and presumably secreted; beta-chemokine RANTES; small inducible cytokine subfamily A (Cys-Cys), member 5; small inducible cytokine A5 (RANTES); chemokine (C-C motif) ligand 5

CCL5 分子靶点综述

趋化因子配体5(CCL5),又名RANTES,是一种趋化细胞因子。它可趋化T细胞、嗜酸性粒细胞和嗜碱性粒细胞,在招募白细胞到炎症位点发挥着积极的作用。在T细胞释放的细胞因子如IL-2和IFN-γ的帮助下,CCL5可诱导某些自然杀伤细胞(NK)增殖和活化形成CC趋化因子活化的杀伤(CHAK)细胞。CCL5也是一种由CD8+T细胞释放的HIV抑制因子。最近,研究者正在开发完善乳酸菌体内产生CCL5的方案,以期其成为抑制HIV进入的局部杀菌剂。

人 Human CCL5 分子靶点信息

  • 分子名:CCL5, C-C motif chemokine ligand 5
  • 别称:
    • beta-chemokine RANTES
    • C-C motif chemokine 5
    • chemokine (C-C motif) ligand 5
    • D17S136E
    • eoCP
    • eosinophil chemotactic cytokine
    • MGC17164
    • RANTES
    • regulated upon activation, normally T-expressed, and presumably secreted
    • SCYA5
    • SIS-delta
    • SISd
    • small inducible cytokine A5 (RANTES)
    • small inducible cytokine subfamily A (Cys-Cys), member 5
    • small-inducible cytokine A5
    • t cell-specific protein P228
    • T-cell specific protein p288
    • T-cell-specific protein RANTES
    • TCP228
  • 基因序列:NCBI_Gene: 6352
  • 蛋白序列:UniProtKB: P13501

人 Human CCL5靶点分子功能(预测)

Enables several functions, including enzyme activator activity; phosphatidylinositol phospholipase C activity; and signaling receptor binding activity. Involved in several processes, including cellular response to cytokine stimulus; positive regulation of cell migration; and positive regulation of cell-cell adhesion. Acts upstream of or within positive regulation of T cell migration. Predicted to be located in cytoplasm. Predicted to be active in extracellular space. Implicated in several diseases, including hepatitis B; hepatitis C; liver disease (multiple); pulmonary tuberculosis; and severe acute respiratory syndrome. Biomarker of several diseases, including fatty liver disease (multiple); glucose metabolism disease (multiple); hypertension (multiple); liver cirrhosis (multiple); and lung disease (multiple).

引用文献统计

该产品被引用的文献总数为:23

Featured ELISA Kit操作常见问题

查看更多Featured ELISA Kit操作步骤技术文章