EK1129

Human CCL5/RANTES ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:CCL5, RANTES, SISd
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:20 μL
  • 灵敏度:0.53 pg/mL
  • 检测范围:15.63 pg/mL - 1000 pg/mL
  • 回收率:84% - 117%

在售SKU:70-EK1129-48, 70-EK1129-96


描述

商品名

Human CCL5/RANTES ELISA Kit (人趋化因子配体5 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:6.9%-7.8%;板间变异系数:5.5% - 8.3%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

20 μL

灵敏度

0.53 pg/mL

检测范围

15.63 pg/mL - 1000 pg/mL

回收率

84% - 117%

平均回收率

0.97

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗CCL5/RANTESA单克隆抗体的96孔聚苯乙烯酶标板
  • 人CCL5/RANTESA冻干标准品
  • CCL5/RANTESA检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人CCL5抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的CCL5与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中CCL5的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human CCL5/RANTES ELISA Kit (人趋化因子配体5 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 CCL5, RANTES, SISd 靶点信息

CCL5 分子靶点信息概述

  • 分子名:CCL5, C-C motif chemokine ligand 5
  • 基因家族:Chemokine ligands
  • 别名:RANTES; SISd; TCP228; MGC17164
  • 曾用名:D17S136E; SCYA5
  • 全称:T-cell specific protein p288; T-cell specific RANTES protein; SIS-delta; regulated upon activation, normally T-expressed, and presumably secreted; beta-chemokine RANTES; small inducible cytokine subfamily A (Cys-Cys), member 5; small inducible cytokine A5 (RANTES); chemokine (C-C motif) ligand 5

CCL5 分子靶点综述

趋化因子配体5(CCL5),又名RANTES,是一种趋化细胞因子。它可趋化T细胞、嗜酸性粒细胞和嗜碱性粒细胞,在招募白细胞到炎症位点发挥着积极的作用。在T细胞释放的细胞因子如IL-2和IFN-γ的帮助下,CCL5可诱导某些自然杀伤细胞(NK)增殖和活化形成CC趋化因子活化的杀伤(CHAK)细胞。CCL5也是一种由CD8+T细胞释放的HIV抑制因子。最近,研究者正在开发完善乳酸菌体内产生CCL5的方案,以期其成为抑制HIV进入的局部杀菌剂。

人 Human CCL5 分子靶点信息

  • 分子名:CCL5, C-C motif chemokine ligand 5
  • 别称:
    • beta-chemokine RANTES
    • C-C motif chemokine 5
    • chemokine (C-C motif) ligand 5
    • D17S136E
    • eoCP
    • eosinophil chemotactic cytokine
    • MGC17164
    • RANTES
    • regulated upon activation, normally T-expressed, and presumably secreted
    • SCYA5
    • SIS-delta
    • SISd
    • small inducible cytokine A5 (RANTES)
    • small inducible cytokine subfamily A (Cys-Cys), member 5
    • small-inducible cytokine A5
    • t cell-specific protein P228
    • T-cell specific protein p288
    • T-cell-specific protein RANTES
    • TCP228
  • 基因序列:NCBI_Gene: 6352
  • 蛋白序列:UniProtKB: P13501

人 Human CCL5靶点分子功能(预测)

Enables several functions, including enzyme activator activity; phosphatidylinositol phospholipase C activity; and signaling receptor binding activity. Involved in several processes, including cellular response to cytokine stimulus; positive regulation of cell migration; and positive regulation of cell-cell adhesion. Acts upstream of or within positive regulation of T cell migration. Predicted to be located in cytoplasm. Predicted to be active in extracellular space. Implicated in several diseases, including hepatitis B; hepatitis C; liver disease (multiple); pulmonary tuberculosis; and severe acute respiratory syndrome. Biomarker of several diseases, including fatty liver disease (multiple); glucose metabolism disease (multiple); hypertension (multiple); liver cirrhosis (multiple); and lung disease (multiple).

操作步骤


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引用文献

文章目录[隐藏]


  1. Endothelial cells promote triple-negative breast cancer cell metastasis via PAI-1 and CCL5 signaling 

  2. Periodontal inflammation recruits distant metastatic breast cancer cells by increasing myeloid-derived suppressor cells 

  3. The combined action of monocytic myeloid-derived suppressor cells and mucosal-associated invariant T cells promotes the progression of cervical cancer 

  4. β-Adrenoceptor regulates contraction and inflammatory cytokine expression of human bladder smooth muscle cells via autophagy under pathological hydrostatic pressure 

  5. Cytokine antibody array-based analysis of IL-37 treatment effects in asthma 

  6. CCR5/CCR5 ligand-induced myeloid-derived suppressor cells are related to the progression of endometriosis 

  7. Metabolic Enzyme Triosephosphate Isomerase 1 and Nicotinamide Phosphoribosyltransferase, Two Independent Inflammatory Indicators in Rheumatoid Arthritis: Evidences From Collagen-Induced Arthritis and Clinical Samples 

  8. FABP6 Expression Correlates with Immune Infiltration and Immunogenicity in Colorectal Cancer Cells 

  9. Increased Expression of Mitochondrial UQCRC1 in Pancreatic Cancer Impairs Antitumor Immunity of Natural Killer Cells via Elevating Extracellular ATP 

  10. FAP-α+ immunofibroblasts in oral lichen planus promote CD4+ T-cell infiltration via CCL5 secretion 

  11. circCYP24A1 facilitates esophageal squamous cell carcinoma progression through binding PKM2 to regulate NF-κB-induced CCL5 secretion 

  12. Changes in peripheral blood cytokines in patients with severe fever with thrombocytopenia syndrome 

  13. Impaired myeloid-derived suppressor cells are associated with recurrent implantation failure: A case-control study 

  14. The kinase activity of integrin-linked kinase regulates cellular senescence in gastric cancer 

  15. Monocytes Undergo Functional Reprogramming to Generate Immunosuppression through HIF-1α Signaling Pathway in the Late Phase of Sepsis 

  16. STING agonist cGAMP enhances anti-tumor activity of CAR-NK cells against pancreatic cancer 

  17. CCL5 promotes the epithelial-mesenchymal transition of circulating tumor cells in renal cancer 

  18. Role of Chemotaxis of Vδ2 T Cells to the Synovium in the Pathogenesis of Acute Gouty Arthritis 

  19. MiR-9 promotes G-MDSC recruitment and tumor proliferation by targeting SOCS3 in breast cancer 

  20. NAMPT/SIRT1 Expression Levels in White Blood Cells Differentiate the Different Rheumatoid Arthritis Subsets: An Inspiration from Traditional Chinese Medicine 

  21. Original research: Chordoma recruits and polarizes tumor-associated macrophages via secreting CCL5 to promote malignant progression 

  22. 腹腔积液 血浆

    CCR5/CCR5 Ligand-Induced Myeloid-Derived Suppressor Cells Are Related to the Progression of Endometriosis 

  23. 细胞培养上清

    FAP-α + immunofibroblasts in oral lichen planus promote CD4 + T-cell infiltration via CCL5 secretion 

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