Human CCL20/MIP-3α ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:CCL20, MIP-3, LARC, ST38
- 种属:人 (Human)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:50 μL
- 灵敏度:8.55 pg/mL
- 检测范围:15.63 pg/mL - 1000 pg/mL
- 回收率:87% - 119%
在售SKU:70-EK1211-48, 70-EK1211-96
描述
商品名 |
Human CCL20/MIP-3α ELISA Kit (人趋化因子配体20 (CCL20) ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:3.8% - 4.5%;板间变异系数:3.2% - 5.3% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
50 μL |
灵敏度 |
8.55 pg/mL |
检测范围 |
15.63 pg/mL - 1000 pg/mL |
回收率 |
87% - 119% |
平均回收率 |
1.01 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人CCL20抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的CCL20与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中CCL20的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
CCL20 分子靶点信息概述
- 分子名:CCL20, C-C motif chemokine ligand 20
- 基因家族:Chemokine ligands
- 别名:LARC; MIP-3a; exodus-1; ST38; CKb4
- 曾用名:SCYA20
- 全称:small inducible cytokine subfamily A (Cys-Cys), member 20; chemokine (C-C motif) ligand 20
CCL20 分子靶点综述
趋化因子配体20(CCL20),又名巨噬细胞炎性蛋白3α(MIP-3α),是CC趋化因子家族的小分子量细胞因子。CCL20对淋巴细胞具有强烈的趋化作用,而对中性粒细胞的趋化作用较弱。单核细胞、巨噬细胞、小胶质细胞、星形胶质细胞、肥大细胞、角质细胞、肠上皮细胞、成骨细胞、成纤维细胞、内皮细胞和支气管上皮细胞都可表达CCL20。CCL20趋化淋巴细胞和树突状细胞到粘膜淋巴组织周围的上皮细胞,从而参与粘膜淋巴组织的形成和功能的发挥。CCL20结合并激活趋化因子受体CCR6来作用于靶细胞。CCL20在中枢神经系统的自身免疫性疾病发病过程中可能起到重要作用。
人 Human CCL20 分子靶点信息
- 分子名:CCL20, C-C motif chemokine ligand 20
- 别称:
- beta chemokine exodus-1
- beta-chemokine exodus-1
- C-C motif chemokine 20
- CC chemokine LARC
- chemokine (C-C motif) ligand 20
- CKb4
- Exodus
- exodus-1
- LARC
- liver and activation-regulated chemokine
- macrophage inflammatory protein 3 alpha
- MIP-3-alpha
- MIP-3a
- MIP3A
- SCYA20
- small inducible cytokine subfamily A (Cys-Cys), member 20
- small-inducible cytokine A20
- ST38
- 基因序列:NCBI_Gene: 6364
- 蛋白序列:UniProtKB: P78556
人 Human CCL20靶点分子功能(预测)
Enables CCR6 chemokine receptor binding activity. Involved in several processes, including antimicrobial humoral immune response mediated by antimicrobial peptide; calcium-mediated signaling using intracellular calcium source; and cell chemotaxis. Acts upstream of or within positive regulation of T cell migration. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Biomarker of several diseases, including IgA glomerulonephritis; Lyme disease; anogenital venereal wart; dermatitis (multiple); and skin papilloma.
操作步骤
文章目录[隐藏]
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引用文献
文章目录[隐藏]
- Enhance anti-lung tumor efficacy of chimeric antigen receptor-T cells by ectopic expression of C–C motif chemokine receptor 6 
- LR12 Promotes Liver Repair by Improving the Resolution of Inflammation and Liver Regeneration in Mice with Thioacetamide- (TAA-) Induced Acute Liver Failure 
- KMT2C Induced by FABP5P3 Aggravates Keratinocyte Hyperproliferation and Psoriasiform Skin Inflammation by Upregulating the Transcription of PIK3R3 
- Identification of CCL20 as a prognostic predictor for severe fever with thrombocytopenia syndrome based on plasma proteomics 
- LPCAT1 facilitates keratinocyte hyperproliferation and skin inflammation in psoriasis via regulating GLUT3 
- Association Between Serum Chemokine Ligand 20 Levels and Disease Activity and Th1/Th2/Th17-Related Cytokine Levels in Rheumatoid Arthritis 
Enhance anti-lung tumor efficacy of chimeric antigen receptor-T cells by ectopic expression of C–C motif chemokine receptor 6 
LR12 Promotes Liver Repair by Improving the Resolution of Inflammation and Liver Regeneration in Mice with Thioacetamide- (TAA-) Induced Acute Liver Failure 
KMT2C Induced by FABP5P3 Aggravates Keratinocyte Hyperproliferation and Psoriasiform Skin Inflammation by Upregulating the Transcription of PIK3R3 
Identification of CCL20 as a prognostic predictor for severe fever with thrombocytopenia syndrome based on plasma proteomics 
LPCAT1 facilitates keratinocyte hyperproliferation and skin inflammation in psoriasis via regulating GLUT3 
Association Between Serum Chemokine Ligand 20 Levels and Disease Activity and Th1/Th2/Th17-Related Cytokine Levels in Rheumatoid Arthritis 
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