EK187

Human MCP-1/CCL2 ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：CCL2, MCP-1, MCAF, SMC-CF
  • 种属：人 (Human)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：50 μL
  • 灵敏度：2.18 pg/mL
  • 检测范围：31.25 pg/mL - 2000 pg/mL
  • 回收率：95% - 118%

在售SKU:70-EK187-48, 70-EK187-96

描述

商品名

Human CCL2/MCP-1 ELISA Kit (人趋化因子配体2 ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：3.8% - 8.8%；板间变异系数：5.3% - 7.1%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

50 μL
灵敏度

2.18 pg/mL
检测范围

31.25 pg/mL - 2000 pg/mL
回收率

95% - 118%
平均回收率

1.07
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗CCL2/MCP-1单克隆抗体的96孔聚苯乙烯酶标板
  • 人CCL2/MCP-1冻干标准品
  • CCL2/MCP-1检测抗体
  • 标准品稀释液
  • 辣根过氧化物酶标记的链霉亲和素
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人CCL2抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的CCL2与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中CCL2的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human CCL2/MCP-1 ELISA Kit (人趋化因子配体2 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 CCL2, MCP-1, MCAF, SMC-CF 靶点信息

CCL2 分子靶点信息概述

  • 分子名：CCL2, C-C motif chemokine ligand 2
  • 基因家族：Chemokine ligands
  • 别名：MCP1; MCP-1; MCAF; SMC-CF; GDCF-2; HC11; MGC9434
  • 曾用名：SCYA2
  • 全称：monocyte chemotactic protein 1, homologous to mouse Sig-je; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; monocyte secretory protein JE; small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); chemokine (C-C motif) ligand 2

CCL2 分子靶点综述

趋化因子配体2(CCL2)，又名单核细胞趋化蛋白1(MCP-1)，是CC趋化因子家族的小分子量细胞因子。CCL2通过蛋白聚糖的粘多糖侧链锚定到内皮细胞的质膜上，主要由单核细胞、巨噬细胞和树突状细胞分泌。CCL2可招募单核细胞、记忆性T细胞和树突状细胞到组织损伤或感染引起的炎症位点。CCL2参与以单核细胞浸润为特征的一些疾病发病机制，如银屑病、类风湿性关节炎和动脉粥样硬化。CCL2参与发生在中枢神经系统的多种疾病的神经炎症过程，表现为神经元变性。

人 Human CCL2 分子靶点信息

  • 分子名：CCL2, C-C motif chemokine ligand 2
  • 别称：
    • C-C motif chemokine 2
    • chemokine (C-C motif) ligand 2
    • GDCF-2
    • HC11
    • HSMCR30
    • MCAF
    • MCP-1
    • MCP1
    • MGC9434
    • monocyte chemoattractant protein 1
    • monocyte chemoattractant protein-1
    • monocyte chemotactic and activating factor
    • monocyte chemotactic protein 1
    • monocyte secretory protein JE
    • SCYA2
    • small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)
    • small inducible cytokine subfamily A (Cys-Cys), member 2
    • small-inducible cytokine A2
    • SMC-CF
  • 基因序列：NCBI_Gene: 6347
  • 蛋白序列：UniProtKB: P13500

人 Human CCL2靶点分子功能（预测）

Predicted to enable CCR2 chemokine receptor binding activity and chemokine activity. Involved in several processes, including cellular response to cytokine stimulus; leukocyte chemotaxis; and regulation of apoptotic process. Located in extracellular region. Implicated in several diseases, including Henoch-Schoenlein purpura; aggressive periodontitis; autoimmune disease (multiple); gastrointestinal system cancer (multiple); and tuberculosis (multiple). Biomarker of several diseases, including autoimmune disease (multiple); liver disease (multiple); lung disease (multiple); non-alcoholic fatty liver disease (multiple); and retinal disease (multiple).

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. Suppression of NOD-like receptor protein 3 inflammasome activation and macrophage M1 polarization by hederagenin contributes to attenuation of sepsis-induced acute lung injury in rats 

  2. Timosaponin B-II alleviates osteoarthritis-related inflammation and extracellular matrix degradation through inhibition of mitogen-activated protein kinases and nuclear factor-κB pathways in vitro 

  3. Fluorous-Tagged Peptide Nanoparticles Ameliorate Acute Lung Injury via Lysosomal Stabilization and Inflammation Inhibition in Pulmonary Macrophages 

  4. Platelet-derived growth factor B attenuates lethal sepsis through inhibition of inflammatory responses 

  5. Effect of ORF7 of SARS-CoV-2 on the Chemotaxis of Monocytes and Neutrophils In Vitro 

  6. Estrogen-Responsive Gene MAST4 Regulates Myeloma Bone Disease 

  7. Periodontal inflammation recruits distant metastatic breast cancer cells by increasing myeloid-derived suppressor cells 

  8. The combined action of monocytic myeloid-derived suppressor cells and mucosal-associated invariant T cells promotes the progression of cervical cancer 

  9. β-Adrenoceptor regulates contraction and inflammatory cytokine expression of human bladder smooth muscle cells via autophagy under pathological hydrostatic pressure 

  10. Single Administration of Ultra-Low-Dose Lipopolysaccharide in Rat Early Pregnancy Induces TLR4 Activation in the Placenta Contributing to Preeclampsia 

  11. Galectin-3 exacerbates ox-LDL-mediated endothelial injury by inducing inflammation via integrin β1-RhoA-JNK signaling activation 

  12. HIC-5 in cancer-associated fibroblasts contributes to esophageal squamous cell carcinoma progression 

  13. Monocyte Chemotactic Protein-1 Regulates Proliferation and Contractility of Human Bladder Smooth Muscle Cells Under Hydrostatic Pressure 

  14. M2 macrophages reduce the effect of gefitinib by activating AKT/mTOR in gefitinib-resistant cell lines HCC827/GR 

  15. Crosstalk of renal cell carcinoma cells and tumor-associated macrophages aggravates tumor progression by modulating muscleblind-like protein 2/B-cell lymphoma 2/beclin 1-mediated autophagy 

  16. Gypenosides Protect Orbital Fibroblasts in Graves Ophthalmopathy via Anti-Inflammation and Anti-Fibrosis Effects 

  17. Baicalein inhibits inflammatory response and promotes osteogenic activity in periodontal ligament cells challenged with lipopolysaccharides 

  18. Insulin Exacerbates Inflammation in Fibroblast-Like Synoviocytes 

  19. Fusobacterium nucleatum stimulates monocyte adhesion to and transmigration through endothelial cells 

  20. Ex vivo lung perfusion with perfusate purification for human donor lungs following prolonged cold storage 

  21. OxLDL/β2GPI/anti‑β2GPI Ab complex induces inflammatory activation via the TLR4/NF‑κB pathway in HUVECs 

  22. Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 

  23. Fenofibrate, a PPARα agonist, protect proximal tubular cells from albumin-bound fatty acids induced apoptosis via the activation of NF-kB 

  24. Changes in peripheral blood cytokines in patients with severe fever with thrombocytopenia syndrome 

  25. The relationship between dyslipidemia and inflammation among adults in east coast China: A cross-sectional study 

  26. Comprehensive Analysis of NAFLD and the Therapeutic Target Identified 

  27. Mesenchymal stem cells pretreated with proinflammatory cytokines accelerate skin wound healing by promoting macrophages migration and M2 polarization 

  28. Isorhamnetin inhibits amplification of influenza A H1N1 virus inflammation mediated by interferon via the RIG-I/JNK pathway 

  29. HIF-1α/JMJD1A signaling regulates inflammation and oxidative stress following hyperglycemia and hypoxia-induced vascular cell injury 

  30. Elevated latent transforming growth factor beta binding protein 2 in endometriosis promotes endometrial stromal cell invasion and proliferation via the NF-kB signaling pathway 

  31. Loss of periodontal ligament fibroblasts by RIPK3-MLKL-mediated necroptosis in the progress of chronic periodontitis 

  32. Porphyromonas gingivalis triggers inflammatory responses in periodontal ligament cells by succinate-succinate dehydrogenase–HIF–1α axis 

  33. NCOA4-mediated ferritinophagy promoted inflammatory responses in periodontitis 

  34. Nesfatin-1 inhibits free fatty acid (FFA)-induced endothelial inflammation via Gfi1/NF-κB signaling 

  35. Impaired myeloid-derived suppressor cells are associated with recurrent implantation failure: A case-control study 

  36. MicroRNA-122-5p regulates coagulation and inflammation through MASP1 and HO-1 genes 

  37. MiR-340 suppresses CCl4-induced acute liver injury through exerting anti-inflammation targeting Sigirr 

  38. Negative impact of maternal depressive symptoms on infancy neurodevelopment: a moderated mediation effect of maternal inflammation 

  39. Development and validation of a nomogram for premature coronary artery disease patients in Guangzhou 

  40. Epithelial cells derived exosomal miR-203a-3p facilitates stromal inflammation of type IIIA chronic prostatitis/chronic pelvic pain syndrome by targeting DUSP5 and increasing MCP-1 generation 

  41. Procyanidin B2 alleviates oxidized low-density lipoprotein-induced cell injury, inflammation, monocyte chemotaxis, and oxidative stress by inhibiting the nuclear factor kappa-B pathway in human umbilical vein endothelial cells 

  42. LncRNA MALAT1 suppresses monocyte-endothelial cell interactions by targeting miR-30b-5p and enhancing ATG5-mediated autophagy 

  43. Innate immune responsive inflammation in development of progressive myopia 

  44. Knockdown of Galectin-9 alleviates rheumatoid arthritis through suppressing TNF-α-induced activation of fibroblast-like synoviocytes 

  45. BMI-specific inflammatory response to phthalate exposure in early pregnancy: findings from the TMCHESC study 

  46. Oxoaporphine Pr(III) complex inhibits hepatocellular carcinoma progression and metastasis by disrupting tumor cell–macrophage crosstalk 

  47. Allicin ameliorates sepsis-induced acute kidney injury through Nrf2/HO-1 signaling pathway 

  48. ATP-citrate lyase controls endothelial gluco-lipogenic metabolism and vascular inflammation in sepsis-associated organ injury 

  49. Angiotensin II type 2 receptor agonist attenuates LPS-induced acute lung injury through modulating THP-1-derived macrophage reprogramming 

  50. Blocking IL-6 signaling improves glucose tolerance via SLC39A5-mediated suppression of glucagon secretion 

  51. LMCD1 is involved in tubulointerstitial inflammation in the early phase of renal fibrosis by promoting NFATc1-mediated NLRP3 activation 

  52. CDYL knockdown reduces glioma development through an antitumor immune response in the tumor microenvironment 

  53. Silibinin attenuated pseudo-allergic reactions and mast cell degranulation via PLCγ and PI3K/Akt signaling pathway 

  54. Multifunctional chondroitin sulfate based hydrogels for promoting infected diabetic wounds healing by chemo-photothermal antibacterial and cytokine modulation 

  55. Dopamine D1 receptor agonist alleviates acute lung injury via modulating inflammatory responses in macrophages and barrier function in airway epithelial cells 

  56. Telotristat Etiprate alleviates rheumatoid arthritis by targeting LGALS3 and affecting MAPK signaling 

  57. Glucokinase Inactivation Ameliorates Lipid Accumulation and Exerts Favorable Effects on Lipid Metabolism in Hepatocytes 

  58. Acteoside alleviates asthma by modulating ROS-responsive NF-κB/MAPK signaling pathway 

  59. Hexokinase 2-mediated glycolysis supports inflammatory responses to Porphyromonas gingivalis in gingival fibroblasts 

  60. 腹腔上清液

    Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 

  61. 细胞培养上清

    Dedifferentiation-associated inflammatory factors of long-term expanded human hepatocytes exacerbate their elimination by macrophages during liver engraftment 

  62. Salvianolic Acid B Inhibits Platelets-Mediated Inflammatory Response in Vascular Endothelial Cells 

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