EK1141S

Human β-NGF Standard (人神经生长因子 (NGF) 标准品)

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¥280.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:NGF, NGFB
  • 种属:人 (Human)
  • 试剂盒:EK1141
  • 保存:短期4℃保存,长期-20℃保存
  • 运输条件:4℃蓝冰运输

在售SKU:70-EK1141S


描述

本产品只包含标准品试剂,如需购买试剂盒请点击下图

商品名

Human β-NGF Standard (人神经生长因子 (NGF) 标准品)

组分

人β-NGFA 标准品

检测方法

双抗夹心法

样本类型

血清,血浆,细胞培养上清及其他生物学样本

板式

保存

短期4℃,长期-20℃保存

运输条件

4℃蓝冰运输

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人β-NGF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的β-NGF与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中β-NGF的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human β-NGF ELISA Kit (人神经生长因子 (NGF) ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 NGF, NGFB 靶点信息

NGF 分子靶点信息概述

  • 分子名:NGF, nerve growth factor
  • 基因家族:Neurotrophins
  • 曾用名:NGFB
  • 全称:nerve growth factor (beta polypeptide)

NGF 分子靶点综述

神经生长因子(NGF)是一种神经肽,主要参与调节某些靶神经元的生长、增殖和存活。当表达时,NGF最初是以7S、分子量为130kDa的复合物存在,这个复合物由α-NGF、β-NGF和γ-NGF(2:1:2比例)三个蛋白组成。“神经生长因子”通常指的是2.5S、分子量为26kDa的β亚基蛋白。β亚基是7SNGF复合物中唯一一个有生物活性的成分(如作为信号分子)。NGF在先天性和获得性免疫中都起着重要作用。研究表明NGF通过血浆在机体中循环,对整体的稳态维持很重要。NGF可促进髓鞘的修复,亦可参与多种精神疾病,如老年痴呆症、抑郁症、精神分裂症、自闭症、雷特综合征、神经性厌食症,神经性贪食症。NGF信号失调与阿尔茨海默病有关。同样,NGF在许多心血管疾病中发挥作用,如冠状动脉粥样硬化、肥胖、2型糖尿病、代谢综合征。NGF也可促进伤口愈合。

人 Human NGF 分子靶点信息

  • 分子名:NGF, nerve growth factor
  • 别称:
    • beta-nerve growth factor
    • Beta-NGF
    • HSAN5
    • MGC161426
    • MGC161428
    • nerve growth factor (beta polypeptide)
    • nerve growth factor, beta polypeptide
    • nerve growth factor, beta subunit
    • NGFB
    • pro-nerve growth factor long
  • 基因序列:NCBI_Gene: 4803
  • 蛋白序列:UniProtKB: P01138

人 Human NGF靶点分子功能(预测)

Enables peptidase regulator activity and signaling receptor binding activity. Involved in extrinsic apoptotic signaling pathway via death domain receptors; neuron apoptotic process; and positive regulation of macromolecule metabolic process. Acts upstream of or within neuron projection morphogenesis. Predicted to be located in neuron projection terminus. Predicted to be active in several cellular components, including axon; dendrite; and synaptic vesicle. Implicated in IgA glomerulonephritis; asthma; end stage renal disease; hereditary sensory and autonomic neuropathy type 5; and interstitial cystitis. Biomarker of several diseases, including artery disease (multiple); interstitial cystitis; lung disease (multiple); neurogenic bladder; and obstructive sleep apnea.

操作步骤

ELISA操作常见问题

查看更多ELISA操作相关问题

引用文献


  1. Colitis aggravated by Mrgprb2 knockout is associated with altered immune response, intestinal barrier function and gut microbiota 

  2. Autocrine TGF-alpha is associated with Benzo(a)pyrene-induced mucus production and MUC5AC expression during allergic asthma 

  3. Effect Evaluation of Strychnos nux-vomica L. with Integrative Methods for Bortezomib-Induced Peripheral Neuropathy in Multiple Myeloma Patients: A Self-Controlled Clinical Trial 

  4. Autophagic Schwann cells promote perineural invasion mediated by the NGF/ATG7 paracrine pathway in pancreatic cancer 

  5. Peripheral Blood Circular RNAs as a Biomarker for Major Depressive Disorder and Prediction of Possible Pathways 

  6. Analgesic Effects of Directed Repetitive Transcranial Magnetic Stimulation in Acute Neuropathic Pain After Spinal Cord Injury 

  7. Serum‑derived exosomes from house dust mite‑sensitized guinea pigs contribute to inflammation in BEAS‑2B cells via the TLR4‑NF‑κB pathway 

  8. Providing biomimetic microenvironment for pulp regeneration via hydrogel-mediated sustained delivery of tissue-specific developmental signals 

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