Human Angiopoietin-1/ANG-1 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:ANGPT1, angiopoietin 1
- 种属:人 (Human)
- 样本类型:血清,血浆,细胞上清
- 检测样本体积:50 μL
- 灵敏度:1.30 pg/mL
- 检测范围:31.25 pg/mL - 2000 pg/mL
- 回收率:80% - 110%
在售SKU:70-EK1122-48, 70-EK1122-96
描述
商品名 |
Human Angiopoietin-1/ANG-1 ELISA Kit (人血管生成素 ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:3.3% - 5.4%;板间变异系数:5.0% - 8.2% |
样本类型 |
血清,血浆,细胞上清 |
检测样本体积 |
50 μL |
灵敏度 |
1.30 pg/mL |
检测范围 |
31.25 pg/mL - 2000 pg/mL |
回收率 |
80% - 110% |
平均回收率 |
0.94 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人ANG-1抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的ANG-1与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中ANG-1的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
ANGPT1 分子靶点信息概述
- 分子名:ANGPT1, angiopoietin 1
- 基因家族:Receptor ligands; Fibrinogen C domain containing
- 别名:KIAA0003; Ang1
ANGPT1 分子靶点综述
血管生成素是血管生长因子家族的一部分,在胚胎和新生血管生成中发挥作用。血管生成素细胞因子通过向血管周围的平滑肌细胞传递信号来调控微血管通透性、血管扩张和血管收缩。血管生成素-1 (ANG-1)编码498个氨基酸多肽,分子量为57 kDa,可形成二聚体、三聚体和四聚体。它在血管成熟、粘附、迁移和生存中发挥重要作用。研究显示血清中ANG-2和ANG-1的比例是增加的,这表明血管生成素和酪氨酸激酶通路的反常在血液相关疾病如糖尿病、疟疾、败血症和肺动脉高压中是常见的。
人 Human ANGPT1 分子靶点信息
- 分子名:ANGPT1, angiopoietin 1
- 别称:
- AGP1
- AGPT
- ANG-1
- ANG1
- angiopoietin-1
- HAE5
- 基因序列:NCBI_Gene: 284
- 蛋白序列:UniProtKB: Q15389
人 Human ANGPT1靶点分子功能(预测)
Enables receptor tyrosine kinase binding activity. Involved in several processes, including positive regulation of intracellular signal transduction; positive regulation of macromolecule metabolic process; and regulation of vascular permeability. Located in several cellular components, including extracellular space; membrane raft; and microvillus. Implicated in brain ischemia; diabetic retinopathy; and hypertension. Biomarker of carcinoma (multiple); hypertension; and ovarian cancer.
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
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- ELISA通关必备丨如何选择试剂盒 
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ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
文章目录[隐藏]
- A novel long-term intravenous combined with local treatment with human amnion-derived mesenchymal stem cells for a multidisciplinary rescued uremic calciphylaxis patient and the underlying mechanism 
- HIF-1α overexpression in mesenchymal stem cell-derived exosomes mediates cardioprotection in myocardial infarction by enhanced angiogenesis 
- Human amnion-derived mesenchymal stem cells improved the reproductive function of age-related diminished ovarian reserve in mice through Ampk/FoxO3a signaling pathway 
- α-Mangostin Alleviated HIF-1α-Mediated Angiogenesis in Rats With Adjuvant-Induced Arthritis by Suppressing Aerobic Glycolysis. 
- System Analysis Based on Lipid-Metabolism-Related Genes Identifies AGT as a Novel Therapy Target for Gastric Cancer with Neoadjuvant Chemotherapy 
A novel long-term intravenous combined with local treatment with human amnion-derived mesenchymal stem cells for a multidisciplinary rescued uremic calciphylaxis patient and the underlying mechanism 
HIF-1α overexpression in mesenchymal stem cell-derived exosomes mediates cardioprotection in myocardial infarction by enhanced angiogenesis 
Human amnion-derived mesenchymal stem cells improved the reproductive function of age-related diminished ovarian reserve in mice through Ampk/FoxO3a signaling pathway 
α-Mangostin Alleviated HIF-1α-Mediated Angiogenesis in Rats With Adjuvant-Induced Arthritis by Suppressing Aerobic Glycolysis. 
System Analysis Based on Lipid-Metabolism-Related Genes Identifies AGT as a Novel Therapy Target for Gastric Cancer with Neoadjuvant Chemotherapy 
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