EK195

Human Adiponectin ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:ADIPOQ, ACRP30, GBP28
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:50 μL
  • 灵敏度:0.44 pg/mL
  • 检测范围:62.5 pg/mL - 4000 pg/mL
  • 回收率:95% - 106%

在售SKU:70-EK195-48, 70-EK195-96


描述

商品名

Human Adiponectin/Acrp30 ELISA Kit (人脂联素 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:2.2% - 5.8%;板间变异系数:2.3% - 6.0%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

50 μL

灵敏度

0.44 pg/mL

检测范围

62.5 pg/mL - 4000 pg/mL

回收率

95% - 106%

平均回收率

0.99

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗Adiponectin/Acrp30单克隆抗体的96孔聚苯乙烯酶标板
  • 人 Adiponectin/Acrp30冻干标准品
  • Adiponectin/Acrp30检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人Adiponectin抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的Adiponectin与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中Adiponectin的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human Adiponectin/Acrp30 ELISA Kit (人脂联素 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 ADIPOQ, ACRP30, GBP28 靶点信息

ADIPOQ 分子靶点信息概述

  • 分子名:ADIPOQ, adiponectin, C1Q and collagen domain containing
  • 基因家族:C1q and TNF related
  • 别名:ACRP30; apM1; GBP28; adiponectin
  • 曾用名:ACDC
  • 全称:adipose most abundant gene transcript 1; adiponectin precursor; adipocyte, C1Q and collagen domain containing

ADIPOQ 分子靶点综述

脂联素,又称脂肪细胞补体相关蛋白30(Acrp30),参与调节葡萄糖水平和脂肪酸分解。它组装成不同的复合物包括三聚体、六聚体和高阶寡聚体结构,进而可能影响生物活性。脂联素是一种蛋白质激素,调节许多代谢过程,包括葡萄糖调节和脂肪酸氧化。脂肪组织(以及妊娠期的胎盘)专门分泌脂联素到血液中,因此相对于其它许多激素,血浆中的脂联素含量很丰富。脂联素可能具有抗动脉粥样硬化和消炎的作用,可负向调节粒单祖细胞的生长和TNF-α在巨噬细胞中的产生。监测一些病理状态下的血清脂联素水平,如2型糖尿病、肥胖、动脉粥样硬化和非酒精性脂肪肝疾病,可为我们了解脂联素提供更详细的见解。

人 Human ADIPOQ 分子靶点信息

  • 分子名:ADIPOQ, adiponectin, C1Q and collagen domain containing
  • 别称:
    • 30 kDa adipocyte complement-related protein
    • ACDC
    • ACRP30
    • adipocyte complement-related 30 kDa protein
    • adiponectin
    • adipose most abundant gene transcript 1 protein
    • adipose specific collagen-like factor
    • ADIPQTL1
    • ADPN
    • APM-1
    • APM1
    • GBP28
    • gelatin-binding protein 28
  • 基因序列:NCBI_Gene: 9370
  • 蛋白序列:UniProtKB: Q15848

人 Human ADIPOQ靶点分子功能(预测)

Enables hormone activity; protein homodimerization activity; and sialic acid binding activity. Involved in several processes, including negative regulation of cell communication; negative regulation of cell differentiation; and negative regulation of macromolecule metabolic process. Located in cell surface and extracellular space. Part of collagen-containing extracellular matrix. Implicated in several diseases, including carcinoma (multiple); cardiovascular system disease (multiple); non-alcoholic fatty liver disease; primary open angle glaucoma; and type 2 diabetes mellitus. Biomarker of several diseases, including autoimmune disease (multiple); cardiovascular system disease (multiple); diabetes mellitus (multiple); fatty liver disease (multiple); and inflammatory bowel disease (multiple).

引用文献统计

该产品被引用的文献总数为:7

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引用文献


  1. MiR-21 antagomir improves insulin resistance and lipid metabolism disorder in streptozotocin-induced type 2 diabetes mellitus rats 

  2. Insulin Resistance Is Associated with Interleukin 1β (IL-1β) in Non-Diabetic Hemodialysis Patients 

  3. Concentrated fish oil ameliorates non-alcoholic fatty liver disease by regulating fibroblast growth factor 21–adiponectin axis 

  4. Associations of Abdominal Visceral Fat Content and Plasma Adiponectin Level With Intracranial Atherosclerotic Stenosis: A Cross-Sectional Study 

  5. Single-cell transcriptome reveals highly complement activated microglia cells in association with pediatric tuberculous meningitis 

  6. NAMPT/SIRT1 Expression Levels in White Blood Cells Differentiate the Different Rheumatoid Arthritis Subsets: An Inspiration from Traditional Chinese Medicine 

  7. Early prediction of body composition parameters on metabolically unhealthy in the Chinese population via advanced machine learning