Human Adiponectin ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:ADIPOQ, ACRP30, GBP28
- 种属:人 (Human)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:50 μL
- 灵敏度:0.44 pg/mL
- 检测范围:62.5 pg/mL - 4000 pg/mL
- 回收率:95% - 106%
在售SKU:70-EK195-48, 70-EK195-96
描述
商品名 |
Human Adiponectin/Acrp30 ELISA Kit (人脂联素 ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:2.2% - 5.8%;板间变异系数:2.3% - 6.0% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
50 μL |
灵敏度 |
0.44 pg/mL |
检测范围 |
62.5 pg/mL - 4000 pg/mL |
回收率 |
95% - 106% |
平均回收率 |
0.99 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人Adiponectin抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的Adiponectin与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中Adiponectin的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
ADIPOQ 分子靶点信息概述
- 分子名:ADIPOQ, adiponectin, C1Q and collagen domain containing
- 基因家族:C1q and TNF related
- 别名:ACRP30; apM1; GBP28; adiponectin
- 曾用名:ACDC
- 全称:adipose most abundant gene transcript 1; adiponectin precursor; adipocyte, C1Q and collagen domain containing
ADIPOQ 分子靶点综述
脂联素,又称脂肪细胞补体相关蛋白30(Acrp30),参与调节葡萄糖水平和脂肪酸分解。它组装成不同的复合物包括三聚体、六聚体和高阶寡聚体结构,进而可能影响生物活性。脂联素是一种蛋白质激素,调节许多代谢过程,包括葡萄糖调节和脂肪酸氧化。脂肪组织(以及妊娠期的胎盘)专门分泌脂联素到血液中,因此相对于其它许多激素,血浆中的脂联素含量很丰富。脂联素可能具有抗动脉粥样硬化和消炎的作用,可负向调节粒单祖细胞的生长和TNF-α在巨噬细胞中的产生。监测一些病理状态下的血清脂联素水平,如2型糖尿病、肥胖、动脉粥样硬化和非酒精性脂肪肝疾病,可为我们了解脂联素提供更详细的见解。
人 Human ADIPOQ 分子靶点信息
- 分子名:ADIPOQ, adiponectin, C1Q and collagen domain containing
- 别称:
- 30 kDa adipocyte complement-related protein
- ACDC
- ACRP30
- adipocyte complement-related 30 kDa protein
- adiponectin
- adipose most abundant gene transcript 1 protein
- adipose specific collagen-like factor
- ADIPQTL1
- ADPN
- APM-1
- APM1
- GBP28
- gelatin-binding protein 28
- 基因序列:NCBI_Gene: 9370
- 蛋白序列:UniProtKB: Q15848
人 Human ADIPOQ靶点分子功能(预测)
Enables hormone activity; protein homodimerization activity; and sialic acid binding activity. Involved in several processes, including negative regulation of cell communication; negative regulation of cell differentiation; and negative regulation of macromolecule metabolic process. Located in cell surface and extracellular space. Part of collagen-containing extracellular matrix. Implicated in several diseases, including carcinoma (multiple); cardiovascular system disease (multiple); non-alcoholic fatty liver disease; primary open angle glaucoma; and type 2 diabetes mellitus. Biomarker of several diseases, including autoimmune disease (multiple); cardiovascular system disease (multiple); diabetes mellitus (multiple); fatty liver disease (multiple); and inflammatory bowel disease (multiple).
操作步骤
文章目录[隐藏]
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引用文献
文章目录[隐藏]
- MiR-21 antagomir improves insulin resistance and lipid metabolism disorder in streptozotocin-induced type 2 diabetes mellitus rats 
- Insulin Resistance Is Associated with Interleukin 1β (IL-1β) in Non-Diabetic Hemodialysis Patients 
- Concentrated fish oil ameliorates non-alcoholic fatty liver disease by regulating fibroblast growth factor 21–adiponectin axis 
- Associations of Abdominal Visceral Fat Content and Plasma Adiponectin Level With Intracranial Atherosclerotic Stenosis: A Cross-Sectional Study 
- Single-cell transcriptome reveals highly complement activated microglia cells in association with pediatric tuberculous meningitis 
- NAMPT/SIRT1 Expression Levels in White Blood Cells Differentiate the Different Rheumatoid Arthritis Subsets: An Inspiration from Traditional Chinese Medicine 
- Early prediction of body composition parameters on metabolically unhealthy in the Chinese population via advanced machine learning 
MiR-21 antagomir improves insulin resistance and lipid metabolism disorder in streptozotocin-induced type 2 diabetes mellitus rats 
Insulin Resistance Is Associated with Interleukin 1β (IL-1β) in Non-Diabetic Hemodialysis Patients 
Concentrated fish oil ameliorates non-alcoholic fatty liver disease by regulating fibroblast growth factor 21–adiponectin axis 
Associations of Abdominal Visceral Fat Content and Plasma Adiponectin Level With Intracranial Atherosclerotic Stenosis: A Cross-Sectional Study 
Single-cell transcriptome reveals highly complement activated microglia cells in association with pediatric tuberculous meningitis 
NAMPT/SIRT1 Expression Levels in White Blood Cells Differentiate the Different Rheumatoid Arthritis Subsets: An Inspiration from Traditional Chinese Medicine 
Early prediction of body composition parameters on metabolically unhealthy in the Chinese population via advanced machine learning 
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