Goat Anti-Rabbit IgG(H+L) HRP 山羊抗兔二抗-HRP
¥289.00 – ¥350.00
在售SKU:70-GAR0072, 70-GAR007, Goat, Rabbit, IgG, HRP, 山羊, 二抗, IgG(H+L), 山羊抗兔, 羊抗兔, 二抗-HRP
描述
背景简介
第二抗体是能和抗体结合,即抗体的抗体,其主要作用是检测抗体的存在,放大一抗的信号。二抗是利用抗体是大分子的蛋白质具有抗原性的性质,去免疫异种动物,由异种动物的免疫系统产生的针对于此抗体的免疫球蛋白。
产品特点
- 性价比高,推荐稀释比例:1:500—1:5000(IHC)、1:5000—1:100000(WB/ELISA)
- 无需分装不冻融
- 价格便宜,常备现货
如何选择二抗
通常情况下,某一实验中可能同时有几种二抗可供选择,如何能选择到最适合该实验的二抗,需要综合以下几个方面进行考虑:
一、 一抗的种属来源
一抗是什么物种来源的,相应的二抗也要是抗该物种的抗体。
二、 一抗的免疫球蛋白亚型
二抗需与一抗的类别或亚型相匹配。例如一抗是IgM,则须选择抗IgM的二抗。如果一抗是某一亚型(如IgG1、IgG2a、IgG2b、IgG3、IgG4等),则可选择能与所有亚型结合的抗IgG二抗,也可选择专门针对该亚型的二抗。
三、 实验方案
根据实验,选择酶标记(如HRP)的二抗,还是荧光素标记的二抗。根据仪器的激发光和检测通道,选择合适的荧光素。
四、 选择哪种形式的二抗,是整个分子还是F[ab’]2片段?
- 整个IgG分子:此种抗体适用于多数情况。
- F[ab’]2片段:该抗体由 2 个二硫键连接的 Fab 片段组成,用于特定的情况中,如需要避免抗体与具有Fc 受体的细胞结合,降低非特异性背景。
引用文献
CGA ameliorates cognitive decline by regulating the PI3K/AKT signaling pathway and neurotransmitter systems in rats with multi-infarct dementia 
Activation of autophagy during farnesyl pyrophosphate synthase inhibition is mediated through PI3K/AKT/mTOR signaling 
Determining 5HT7R’s Involvement in Modifying the Antihyperalgesic Effects of Electroacupuncture on Rats With Recurrent Migraine 
Exploring and Verifying the Mechanism and Targets of Shenqi Pill in the Treatment of Nonalcoholic Steatohepatitis via Network Pharmacology and Experiments 
Blood-brain barrier integrity is the primary target of alcohol abuse 
Long-term consumption of alcohol exacerbates neural lesions by destroying the functional integrity of the blood–brain barrier 
Shenqi Yizhi Granule attenuates Aβ1–42 induced cognitive dysfunction via inhibiting JAK2/STAT3 activated astrocyte reactivity 
Effect of Forsythiaside A on the RLRs Signaling Pathway in the Lungs of Mice Infected with the Influenza A Virus FM1 Strain 
To Modulate Survival under Secondary Stress Conditions, Listeria monocytogenes 10403S Employs RsbX To Downregulate σB Activity in the Poststress Recovery Stage or Stationary Phase 
Drinking water temperature affects cognitive function and progression of Alzheimer’s disease in a mouse model 
Self-assembling Bioadhesive Inspired by the Fourth Repetitive Sequence of Balanus albicostatus Cement Protein 20 kDa (Balcp-20 k) 
Evaluation of the immunomodulatory effects of C9-13-CPs in macrophages 
Follistatin-like 3, an activin A binding protein, is involved in early pregnancy loss 
Study on the Chemical Constituents and Anti-Migraine Activity of Supercritical CO2 Extracts of Zanthoxylum schinifolium 
Effects of different principles of Traditional Chinese Medicine treatment on TLR7/NF-κB signaling pathway in influenza virus infected mice 
Chinese herbal component, Praeruptorin E, enhances anti-asthma efficacy and prevents toxicity of aminophylline by targeting the NF-κB/PXR/CYP3A4 pathway 
PDGFD switches on stem cell endothelial commitment 
Effect of rifaximin on gut-lung axis in mice infected with influenza A virus 
Autophagy in Retinal Ganglion Cells in a Rhesus Monkey Chronic Hypertensive Glaucoma Model 
Enhanced chemotherapeutic efficacy of the low-dose doxorubicin in breast cancer via nanoparticle delivery system crosslinked hyaluronic acid 
Osteocytic cells exposed to titanium particles increase sclerostin expression and inhibit osteoblastic cell differentiation mostly via direct cell-to-cell contact 
Rosuvastatin Reverses Hypertension-Induced Changes in the Aorta Structure and Endothelium-Dependent Relaxation in Rats Through Suppression of Apoptosis and Inflammation 
Selective preservation of cholinergic MeCP2 rescues specific Rett-syndrome-like phenotypes in MeCP2stop mice 
Environmentally relevant doses of tetrabromobisphenol A (TBBPA) cause immunotoxicity in murine macrophages 
SiBaoChongCao exhibited anti-fatigue activities and ameliorated cancer cachexia in mice 
Prenatal hypothyroidism diminished exogenous NO-mediated diastolic effects in fetal rat thoracic aorta smooth muscle via increased oxidative stress 
Oncolytic Vaccinia Virus Expressing Aphrocallistes vastus Lectin as a Cancer Therapeutic Agent 
Maternal Plasma l-Carnitine Reduction During Pregnancy Is Mainly Attributed to OCTN2-Mediated Placental Uptake and Does Not Result in Maternal Hepatic Fatty Acid β-Oxidation Decline 
Genome-wide screening of budding yeast with honokiol to associate mitochondrial function with lipid metabolism 
Establishment of a mouse model of cancer cachexia with spleen deficiency syndrome and the effects of atractylenolide I 
Bisdemethoxycurcumin alleviates vandetanib-induced cutaneous toxicity in vivo and in vitro through autophagy activation 
HMGB1 represses the anti-cancer activity of sunitinib by governing TP53 autophagic degradation via its nucleus-to-cytoplasm transport 
PLK1 (polo like kinase 1)-dependent autophagy facilitates gefitinib-induced hepatotoxicity by degrading COX6A1 (cytochrome c oxidase subunit 6A1) 
Mitochondrial pathway‑mediated apoptosis is associated with erlotinib‑induced cytotoxicity in hepatic cells 
Autophagic degradation of CCN2 (cellular communication network factor 2) causes cardiotoxicity of sunitinib 
Imperatorin alleviated endometriosis by inhibiting the activation of PI3K/Akt/NF-κB pathway in rats 
Pyrogallol enhances therapeutic effect of human umbilical cord mesenchymal stem cells against LPS-mediated inflammation and lung injury via activation of Nrf2/HO-1 signaling 
Dasatinib causes keratinocyte apoptosis via inhibiting high mobility group Box 1-mediated mitophagy 
Triptolide protects against white matter injury induced by chronic cerebral hypoperfusion in mice 
uc.338 targets p21 and cyclin D1 via PI3K/AKT pathway activation to promote cell proliferation in colorectal cancer 
TRIM27 functions as an oncogene by activating epithelial-mesenchymal transition and p-AKT in colorectal cancer 
Generation of an anti-angiogenic endothelial progenitor cell line via endostatin gene transfer 
Resveratrol protects HaCaT cells from ultraviolet B-induced photoaging via upregulation of HSP27 and modulation of mitochondrial caspase-dependent apoptotic pathway 
Functional Characterization of Human Peptide/Histidine Transporter 1 in Stably Transfected MDCK Cells 
Crosstalk between apoptosis and autophagy in prostate epithelial cells under androgen deprivation 
TNF-α promotes survival and migration of MSCs under oxidative stress via NF-κB pathway to attenuate intimal hyperplasia in vein grafts 
The anti-inflammatory NHE-06 restores antitumor immunity by targeting NF-κB/IL-6/STAT3 signaling in hepatocellular carcinoma 
Novel germline mutation KMT2A G3131S confers genetic susceptibility to familial myeloproliferative neoplasms 
Dichloroacetate blocks aerobic glycolytic adaptation to attenuated measles virus and promotes viral replication leading to enhanced oncolysis in glioblastoma 
Functional Role of SUV39H1 in Human Renal Tubular Epithelial Cells Under High-glucose Ambiance 
Human Umbilical Cord Mesenchymal Stem Cell Derived Exosomes Delivered Using Silk Fibroin and Sericin Composite Hydrogel Promote Wound Healing 
Netrin‑1 induces the proliferation of gastric cancer cells via the ERK/MAPK signaling pathway and FAK activation Corrigendum in /10.3892/or.2018.6863 
Energy sensor AMPK gamma regulates translation via phosphatase PPP6C independent of AMPK alpha 
Recruitment of tetraspanin TSP-15 to epidermal wounds promotes plasma membrane repair in C. elegans 
Tetrahydrocurcumin‑induced autophagy via suppression of PI3K/Akt/mTOR in non‑small cell lung carcinoma cells 
Oncolytic vaccine virus harbouring the IL-24 gene suppresses the growth of lung cancer by inducing apoptosis 
Reverse of Acute and Chronic Morphine Tolerance by Lithocholic Acid via Down-Regulating UGT2B7 
Tachypleus tridentatus Lectin Enhances Oncolytic Vaccinia Virus Replication to Suppress In Vivo Hepatocellular Carcinoma Growth 
N-Oleoylethanolamine Reduces Inflammatory Cytokines and Adhesion Molecules in TNF-α-induced Human Umbilical Vein Endothelial Cells by Activating CB2 and PPAR-α 
NBR1 mediates autophagic degradation of IRF3 to negatively regulate type I interferon production 
Inhibition of Sirtuin 3 prevents titanium particle-induced bone resorption and osteoclastsogenesis via suppressing ERK and JNK signaling 
Development and Validation of a Three-gene Prognostic Signature for Patients with Hepatocellular Carcinoma 
The Candidate Tumor Suppressor Gene SLC8A2 Inhibits Invasion, Angiogenesis and Growth of Glioblastoma 
Enhanced Expression of Markers for Astrocytes in the Brain of a Line of GFAP-TK Transgenic Mice 
Synergistic antitumor effects of CDK inhibitor SNS‑032 and an oncolytic adenovirus co‑expressing TRAIL and Smac in pancreatic cancer 
Attenuation of Activated eIF2α Signaling by ISRIB Treatment After Spinal Cord Injury Improves Locomotor Function 
DUSP1 induces apatinib resistance by activating the MAPK pathway in gastric cancer 
DL‑3‑n‑butylphthalide protects H9c2 cardiomyoblasts from ischemia/reperfusion injury by regulating HSP70 expression via PI3K/AKT pathway activation 
A novel HPV16 E7-affitoxin for targeted therapy of HPV16-induced human cervical cancer 
Bone morphogenetic protein 4 inhibits rat stem/progenitor Leydig cell development and regeneration via SMAD-dependent and SMAD-independent signaling 
In utero bisphenol AF exposure causes fetal Leydig cell dysfunction and induces multinucleated gonocytes by generating oxidative stress and reducing the SIRT1/PGC1α signals 
Revealing chemical processes and kinetics of drug action within single living cells via plasmonic Raman probes 
Inhibition of Histone Deacetylase 6 by Tubastatin A Attenuates the Progress of Osteoarthritis via Improving Mitochondrial Function 
Berberine Protects Against Palmitate-Induced Apoptosis in Tubular Epithelial Cells by Promoting Fatty Acid Oxidation 
Decreased Expression of HOXA10 May Activate the Autophagic Process in Ovarian Endometriosis 
Autophagy Protects against Palmitic Acid-Induced Apoptosis in Podocytes in vitro 
Amyloid β Is Not the Major Factor Accounting for Impaired Adult Hippocampal Neurogenesis in Mice Overexpressing Amyloid Precursor Protein 
Deletion of ribosomal paralogs Rpl39 and Rpl39l compromises cell proliferation via protein synthesis and mitochondrial activity 
Hypermethylation of PRKCZ Regulated by E6 Inhibits Invasion and EMT via Cdc42 in HPV-Related Head and Neck Squamous Cell Carcinoma 
Treatment of an autoimmune encephalomyelitis mouse model with nonmyeloablative conditioning and syngeneic bone marrow transplantation 
Subicular Caspase-1 Contributes to Pharmacoresistance in Temporal Lobe Epilepsy 
Uncovering the mechanism of Huanglian-Wuzhuyu herb pair in treating nonalcoholic steatohepatitis based on network pharmacology and experimental validation 
The signal peptide of Cry1Ia can improve the expression of eGFP or mCherry in Escherichia coli and Bacillus thuringiensis and enhance the host’s fluorescent intensity 
Reduced expression of PinX1 correlates to progressive features in patients with prostate cancer 
Mitochondrial dysfunction induces NLRP3 inflammasome activation during cerebral ischemia/reperfusion injury 
Anemoside B4 prevents chronic obstructive pulmonary disease through alleviating cigarette smoke-induced inflammatory response and airway epithelial hyperplasia 
Hydroxysafflor Yellow A Reprograms TLR9 Signalling Pathway in Ischaemic Cortex after Cerebral Ischaemia and Reperfusion 
EIF5A expression and its role as a potential diagnostic biomarker in hepatocellular carcinoma 
LncRNA OIP5-AS1 Promotes the Autophagy-Related Imatinib Resistance in Chronic Myeloid Leukemia Cells by Regulating miR-30e-5p/ATG12 Axis 
Aberrant Serotonergic Signaling Contributes to the Hyperexcitability of CA1 Pyramidal Neurons in a Mouse Model of Alzheimer's Disease 
5-Methoxyflavone alleviates LPS-mediated lung injury by promoting Nrf2-mediated the suppression of NOX4/TLR4 axis in bronchial epithelial cells and M1 polarization in macrophages 
Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts 
Overexpression of COPS3 promotes clear cell renal cell carcinoma progression via regulation of Phospho-AKT(Thr308), Cyclin D1 and Caspase-3 
NOVA1 promotes NSCLC proliferation and invasion by activating Wnt/β-catenin signaling 
Klotho downregulation contributes to myocardial damage of cardiorenal syndrome in sepsis 
25-HC decreases the sensitivity of human gastric cancer cells to 5-fluorouracil and promotes cells invasion via the TLR2/NF-κB signaling pathway 
The role of miR-431-5p in regulating pulmonary surfactant expression in vitro 
Increased Thioredoxin-1 Expression Promotes Cancer Progression and Predicts Poor Prognosis in Patients with Gastric Cancer 
Decreased HMGB1 expression contributed to cutaneous toxicity caused by lapatinib 
Evaluation of a bivalent recombinant vaccine candidate targeting norovirus and rotavirus: Antibodies to rotavirus NSP4 exert antidiarrheal effects without virus neutralization 
Transcriptional silencing of ETS-1 abrogates epithelial-mesenchymal transition resulting in reduced motility of pancreatic cancer cells 
Delivery of miR-675 by stem cell-derived exosomes encapsulated in silk fibroin hydrogel prevents aging-induced vascular dysfunction in mouse hindlimb 
Proteomic analysis of strawberry leaves infected with Colletotrichum fragariae 
High expression of TMEM40 contributes to progressive features of tongue squamous cell carcinoma 
Redox-sensitive CDC-42 clustering promotes wound closure in C. elegans 
Inhibition of farnesyl pyrophosphate synthase attenuates angiotensin II-induced fibrotic responses in vascular smooth muscle cells 
Seminal plasma miR-192a: a biomarker predicting successful resolution of nonobstructive azoospermia following varicocele repair 
Promoting effect of the Maillard reaction products produced during the stir-frying process of Hordei Fructus Germinatus on the intestinal absorption of active ingredients in Hordei Fructus Germinatus 
The Role of Caveolin-1 in Blood–Brain Barrier Disruption Induced by Focused Ultrasound Combined with Microbubbles 
Thiazolidinediones improve hepatic fibrosis in rats with non-alcoholic steatohepatitis by activating the adenosine monophosphate-activated protein kinase signalling pathway 
Rapid isolation and proteome analysis of urinary exosome based on double interactions of Fe3O4@TiO2-DNA aptamer 
Phenformin Down-Regulates c-Myc Expression to Suppress the Expression of Pro-Inflammatory Cytokines in Keratinocytes 
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(-)-Epigallocatechin-3-gallate Ameliorates Intervertebral Disc Degeneration Through Reprogramming of the Circadian Clock. 
Augmented anti-tumor activity of NK-92 cells expressing chimeric receptors of TGF-βR II and NKG2D 
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