GAR007

Goat Anti-Rabbit IgG(H+L) HRP 山羊抗兔二抗-HRP

X

¥289.00¥350.00

在售SKU:70-GAR0072, 70-GAR007, Goat, Rabbit, IgG, HRP, 山羊, 二抗, IgG(H+L), 山羊抗兔, 羊抗兔, 二抗-HRP


描述

背景简介

第二抗体是能和抗体结合,即抗体的抗体,其主要作用是检测抗体的存在,放大一抗的信号。二抗是利用抗体是大分子的蛋白质具有抗原性的性质,去免疫异种动物,由异种动物的免疫系统产生的针对于此抗体的免疫球蛋白。

产品特点

  • 性价比高,推荐稀释比例:1:500—1:5000(IHC)、1:5000—1:100000(WB/ELISA)
  • 无需分装不冻融
  • 价格便宜,常备现货

如何选择二抗

通常情况下,某一实验中可能同时有几种二抗可供选择,如何能选择到最适合该实验的二抗,需要综合以下几个方面进行考虑:

一、 一抗的种属来源

一抗是什么物种来源的,相应的二抗也要是抗该物种的抗体。

二、 一抗的免疫球蛋白亚型

二抗需与一抗的类别或亚型相匹配。例如一抗是IgM,则须选择抗IgM的二抗。如果一抗是某一亚型(如IgG1、IgG2a、IgG2b、IgG3、IgG4等),则可选择能与所有亚型结合的抗IgG二抗,也可选择专门针对该亚型的二抗。

三、 实验方案

根据实验,选择酶标记(如HRP)的二抗,还是荧光素标记的二抗。根据仪器的激发光和检测通道,选择合适的荧光素。

四、 选择哪种形式的二抗,是整个分子还是F[ab’]2片段?

  • 整个IgG分子:此种抗体适用于多数情况。
  • F[ab’]2片段:该抗体由 2 个二硫键连接的 Fab 片段组成,用于特定的情况中,如需要避免抗体与具有Fc 受体的细胞结合,降低非特异性背景。

引用文献

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  2. Activation of autophagy during farnesyl pyrophosphate synthase inhibition is mediated through PI3K/AKT/mTOR signaling 

  3. Determining 5HT7R’s Involvement in Modifying the Antihyperalgesic Effects of Electroacupuncture on Rats With Recurrent Migraine 

  4. Exploring and Verifying the Mechanism and Targets of Shenqi Pill in the Treatment of Nonalcoholic Steatohepatitis via Network Pharmacology and Experiments 

  5. Blood-brain barrier integrity is the primary target of alcohol abuse 

  6. Long-term consumption of alcohol exacerbates neural lesions by destroying the functional integrity of the blood–brain barrier 

  7. Shenqi Yizhi Granule attenuates Aβ1–42 induced cognitive dysfunction via inhibiting JAK2/STAT3 activated astrocyte reactivity 

  8. Effect of Forsythiaside A on the RLRs Signaling Pathway in the Lungs of Mice Infected with the Influenza A Virus FM1 Strain 

  9. To Modulate Survival under Secondary Stress Conditions, Listeria monocytogenes 10403S Employs RsbX To Downregulate σB Activity in the Poststress Recovery Stage or Stationary Phase 

  10. Drinking water temperature affects cognitive function and progression of Alzheimer’s disease in a mouse model 

  11. Self-assembling Bioadhesive Inspired by the Fourth Repetitive Sequence of Balanus albicostatus Cement Protein 20 kDa (Balcp-20 k) 

  12. Evaluation of the immunomodulatory effects of C9-13-CPs in macrophages 

  13. Follistatin-like 3, an activin A binding protein, is involved in early pregnancy loss 

  14. Study on the Chemical Constituents and Anti-Migraine Activity of Supercritical CO2 Extracts of Zanthoxylum schinifolium 

  15. Effects of different principles of Traditional Chinese Medicine treatment on TLR7/NF-κB signaling pathway in influenza virus infected mice 

  16. Chinese herbal component, Praeruptorin E, enhances anti-asthma efficacy and prevents toxicity of aminophylline by targeting the NF-κB/PXR/CYP3A4 pathway 

  17. PDGFD switches on stem cell endothelial commitment 

  18. Effect of rifaximin on gut-lung axis in mice infected with influenza A virus 

  19. Autophagy in Retinal Ganglion Cells in a Rhesus Monkey Chronic Hypertensive Glaucoma Model 

  20. Enhanced chemotherapeutic efficacy of the low-dose doxorubicin in breast cancer via nanoparticle delivery system crosslinked hyaluronic acid 

  21. Osteocytic cells exposed to titanium particles increase sclerostin expression and inhibit osteoblastic cell differentiation mostly via direct cell-to-cell contact 

  22. Rosuvastatin Reverses Hypertension-Induced Changes in the Aorta Structure and Endothelium-Dependent Relaxation in Rats Through Suppression of Apoptosis and Inflammation 

  23. Selective preservation of cholinergic MeCP2 rescues specific Rett-syndrome-like phenotypes in MeCP2stop mice 

  24. Environmentally relevant doses of tetrabromobisphenol A (TBBPA) cause immunotoxicity in murine macrophages 

  25. SiBaoChongCao exhibited anti-fatigue activities and ameliorated cancer cachexia in mice 

  26. Prenatal hypothyroidism diminished exogenous NO-mediated diastolic effects in fetal rat thoracic aorta smooth muscle via increased oxidative stress 

  27. Oncolytic Vaccinia Virus Expressing Aphrocallistes vastus Lectin as a Cancer Therapeutic Agent 

  28. Maternal Plasma l-Carnitine Reduction During Pregnancy Is Mainly Attributed to OCTN2-Mediated Placental Uptake and Does Not Result in Maternal Hepatic Fatty Acid β-Oxidation Decline 

  29. Genome-wide screening of budding yeast with honokiol to associate mitochondrial function with lipid metabolism 

  30. Establishment of a mouse model of cancer cachexia with spleen deficiency syndrome and the effects of atractylenolide I 

  31. Bisdemethoxycurcumin alleviates vandetanib-induced cutaneous toxicity in vivo and in vitro through autophagy activation 

  32. HMGB1 represses the anti-cancer activity of sunitinib by governing TP53 autophagic degradation via its nucleus-to-cytoplasm transport 

  33. PLK1 (polo like kinase 1)-dependent autophagy facilitates gefitinib-induced hepatotoxicity by degrading COX6A1 (cytochrome c oxidase subunit 6A1) 

  34. Mitochondrial pathway‑mediated apoptosis is associated with erlotinib‑induced cytotoxicity in hepatic cells 

  35. Autophagic degradation of CCN2 (cellular communication network factor 2) causes cardiotoxicity of sunitinib 

  36. Imperatorin alleviated endometriosis by inhibiting the activation of PI3K/Akt/NF-κB pathway in rats 

  37. Pyrogallol enhances therapeutic effect of human umbilical cord mesenchymal stem cells against LPS-mediated inflammation and lung injury via activation of Nrf2/HO-1 signaling 

  38. Dasatinib causes keratinocyte apoptosis via inhibiting high mobility group Box 1-mediated mitophagy 

  39. Triptolide protects against white matter injury induced by chronic cerebral hypoperfusion in mice 

  40. uc.338 targets p21 and cyclin D1 via PI3K/AKT pathway activation to promote cell proliferation in colorectal cancer 

  41. TRIM27 functions as an oncogene by activating epithelial-mesenchymal transition and p-AKT in colorectal cancer 

  42. Generation of an anti-angiogenic endothelial progenitor cell line via endostatin gene transfer 

  43. Resveratrol protects HaCaT cells from ultraviolet B-induced photoaging via upregulation of HSP27 and modulation of mitochondrial caspase-dependent apoptotic pathway 

  44. Functional Characterization of Human Peptide/Histidine Transporter 1 in Stably Transfected MDCK Cells 

  45. Crosstalk between apoptosis and autophagy in prostate epithelial cells under androgen deprivation 

  46. TNF-α promotes survival and migration of MSCs under oxidative stress via NF-κB pathway to attenuate intimal hyperplasia in vein grafts 

  47. The anti-inflammatory NHE-06 restores antitumor immunity by targeting NF-κB/IL-6/STAT3 signaling in hepatocellular carcinoma 

  48. Novel germline mutation KMT2A G3131S confers genetic susceptibility to familial myeloproliferative neoplasms 

  49. Dichloroacetate blocks aerobic glycolytic adaptation to attenuated measles virus and promotes viral replication leading to enhanced oncolysis in glioblastoma 

  50. Functional Role of SUV39H1 in Human Renal Tubular Epithelial Cells Under High-glucose Ambiance 

  51. Human Umbilical Cord Mesenchymal Stem Cell Derived Exosomes Delivered Using Silk Fibroin and Sericin Composite Hydrogel Promote Wound Healing 

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  53. Energy sensor AMPK gamma regulates translation via phosphatase PPP6C independent of AMPK alpha 

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  55. Tetrahydrocurcumin‑induced autophagy via suppression of PI3K/Akt/mTOR in non‑small cell lung carcinoma cells 

  56. Oncolytic vaccine virus harbouring the IL-24 gene suppresses the growth of lung cancer by inducing apoptosis 

  57. Reverse of Acute and Chronic Morphine Tolerance by Lithocholic Acid via Down-Regulating UGT2B7 

  58. Tachypleus tridentatus Lectin Enhances Oncolytic Vaccinia Virus Replication to Suppress In Vivo Hepatocellular Carcinoma Growth 

  59. N-Oleoylethanolamine Reduces Inflammatory Cytokines and Adhesion Molecules in TNF-α-induced Human Umbilical Vein Endothelial Cells by Activating CB2 and PPAR-α 

  60. NBR1 mediates autophagic degradation of IRF3 to negatively regulate type I interferon production 

  61. Inhibition of Sirtuin 3 prevents titanium particle-induced bone resorption and osteoclastsogenesis via suppressing ERK and JNK signaling 

  62. Development and Validation of a Three-gene Prognostic Signature for Patients with Hepatocellular Carcinoma 

  63. The Candidate Tumor Suppressor Gene SLC8A2 Inhibits Invasion, Angiogenesis and Growth of Glioblastoma 

  64. Enhanced Expression of Markers for Astrocytes in the Brain of a Line of GFAP-TK Transgenic Mice 

  65. Synergistic antitumor effects of CDK inhibitor SNS‑032 and an oncolytic adenovirus co‑expressing TRAIL and Smac in pancreatic cancer 

  66. Attenuation of Activated eIF2α Signaling by ISRIB Treatment After Spinal Cord Injury Improves Locomotor Function 

  67. DUSP1 induces apatinib resistance by activating the MAPK pathway in gastric cancer 

  68. DL‑3‑n‑butylphthalide protects H9c2 cardiomyoblasts from ischemia/reperfusion injury by regulating HSP70 expression via PI3K/AKT pathway activation 

  69. A novel HPV16 E7-affitoxin for targeted therapy of HPV16-induced human cervical cancer 

  70. Bone morphogenetic protein 4 inhibits rat stem/progenitor Leydig cell development and regeneration via SMAD-dependent and SMAD-independent signaling 

  71. In utero bisphenol AF exposure causes fetal Leydig cell dysfunction and induces multinucleated gonocytes by generating oxidative stress and reducing the SIRT1/PGC1α signals 

  72. Revealing chemical processes and kinetics of drug action within single living cells via plasmonic Raman probes 

  73. Inhibition of Histone Deacetylase 6 by Tubastatin A Attenuates the Progress of Osteoarthritis via Improving Mitochondrial Function 

  74. Berberine Protects Against Palmitate-Induced Apoptosis in Tubular Epithelial Cells by Promoting Fatty Acid Oxidation 

  75. Decreased Expression of HOXA10 May Activate the Autophagic Process in Ovarian Endometriosis 

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  80. Treatment of an autoimmune encephalomyelitis mouse model with nonmyeloablative conditioning and syngeneic bone marrow transplantation 

  81. Subicular Caspase-1 Contributes to Pharmacoresistance in Temporal Lobe Epilepsy 

  82. Uncovering the mechanism of Huanglian-Wuzhuyu herb pair in treating nonalcoholic steatohepatitis based on network pharmacology and experimental validation 

  83. The signal peptide of Cry1Ia can improve the expression of eGFP or mCherry in Escherichia coli and Bacillus thuringiensis and enhance the host’s fluorescent intensity 

  84. Reduced expression of PinX1 correlates to progressive features in patients with prostate cancer 

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  87. Hydroxysafflor Yellow A Reprograms TLR9 Signalling Pathway in Ischaemic Cortex after Cerebral Ischaemia and Reperfusion 

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  92. Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts 

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  112. Phenformin Down-Regulates c-Myc Expression to Suppress the Expression of Pro-Inflammatory Cytokines in Keratinocytes 

  113. DNA Methylation and SNPs in VCX are Correlated with Sex Differences in the Response to Chronic Hepatitis B 

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  115. Inhibition of farnesyl pyrophosphate synthase attenuates high glucose‑induced vascular smooth muscle cells proliferation 

  116. Chronic ethanol treatment of human hepatocytes inhibits the activation of the insulin signaling pathway by increasing cytosolic free calcium levels 

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