EasyGo!™ Rat IL-1β One-Step ELISA Kit 检测试剂盒(酶联免疫吸附法)
¥2,190.00 – ¥3,650.00
- 分子靶点:IL1B, IL1-β, IL1F2
- 种属:大鼠 (Rat)
- 样本类型:血清,血浆
- 检测样本体积:5 μL
- 灵敏度:1.80 pg/mL
- 检测范围:7.81 pg/mL - 500 pg/mL
- 回收率:81% - 123%
在售SKU:70-EK301BEHA-48, 70-EK301BEGA-96, EK301BEGA
描述
商品名 |
EasyGo!™ Rat IL-1β One-Step ELISA Kit 检测试剂盒(酶联免疫吸附法) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:9.4%;板间变异系数:5.8% - 9.0% |
样本类型 |
血清,血浆 |
检测样本体积 |
5 μL |
灵敏度 |
1.80 pg/mL |
检测范围 |
7.81 pg/mL - 500 pg/mL |
回收率 |
81% - 123% |
平均回收率 |
1.00 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,2 - 8℃保存。已拆开,标准品-20℃保存,其它2 - 8℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上,酶标板孔中预先加入生物素标记的微球和辣根过氧化物酶微球。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后,加入显色底物(TMB),避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应,在 450nm 波长(参考波长 5630nm)测定吸光度值。
分子信息
IL1B 分子靶点信息概述
- 分子名:IL1B, interleukin 1 beta
- 基因家族:Interleukins
- 别名:IL1F2; IL-1B; IL1-BETA
IL1B 分子靶点综述
白细胞介素1(IL-1)是17kDa的胞外多肽,分为IL-1α和IL-1β两种蛋白。IL-1β是活化的巨噬细胞分泌的蛋白前体,通过caspase1(CASP1/ICE)水解产生的活化形式。该细胞因子是炎症反应的重要介导者,参与许多细胞活性,包括细胞增殖、分化和凋亡。IL-1是一类重要的蛋白家族,它们由生物活化的单核细胞所衍生,参与炎症反应和免疫应答。IL-1β表达升高会产生各种不同的自身炎症综合征,最显著的是单基因条件称为CAPS,是由于触发IL-1β加工的炎性体受体NLRP3发生突变而引起的。本试剂盒用来检测人IL-1β的成熟形式和未裂解的前体形式。
大鼠 Rat Il1b 分子靶点信息
- 分子名:Il1b, interleukin 1 beta
- 别称:
- IL-1 beta
- IL-1F2
- interleukin-1 beta
- 基因序列:NCBI_Gene: 24494
- 蛋白序列:
大鼠 Rat Il1b 靶点分子功能(预测)
Enables cytokine activity. Involved in several processes, including positive regulation of intracellular signal transduction; regulation of gene expression; and regulation of neurogenesis. Located in extracellular space. Used to study several diseases, including artery disease (multiple); brain disease (multiple); glomerulonephritis (multiple); interstitial lung disease (multiple); and kidney failure (multiple). Biomarker of several diseases, including artery disease (multiple); auditory system disease (multiple); brain disease (multiple); kidney failure (multiple); and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); blood platelet disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Orthologous to human IL1B (interleukin 1 beta).
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
- 血清OR血浆,哪个是ELISA的菜 
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- ELISA通关必备丨基础知识 
- 真?假?ELISA试剂盒选择要小心 
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ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
文章目录[隐藏]
- Cardioprotective Effects of Phlorizin on Hyperlipidemia-induced Myocardial Injury: Involvement of Suppression in Pyroptosis via Regulating HK1/NLRP3/Caspase-1 Signaling Pathway 
- Discovery of CYP2E1 as a novel target in rheumatoid arthritis and validation by a new specific CYP2E1 inhibitor 
- Heat-killed Bifidobacterium longum BBMN68 and inulin protect against high-fat diet-induced obesity by modulating gut microbiota 
- Optical imaging detection of extracellular vesicles of miR-146 modified bone marrow mesenchymal stem cells promoting spinal cord injury repair 
- MICT ameliorates hypertensive nephropathy by inhibiting TLR4/NF-κB pathway and down-regulating NLRC4 inflammasome 
- PM10 exposure induces bronchial hyperresponsiveness by upreguating acetylcholine muscarinic 3 receptor 
- Acupuncture ameliorates depression-like behavior of poststroke depression model rats through the regulation of gut microbiota and NLRP3 inflammasome in the colon 
- Acupuncture ameliorates depression-like behavior of poststroke depression model rats through the regulation of gut microbiota and NLRP3 inflammasome in the colon 
- Acupuncture ameliorates depression-like behavior of poststroke depression model rats through the regulation of gut microbiota and NLRP3 inflammasome in the colon 
Cardioprotective Effects of Phlorizin on Hyperlipidemia-induced Myocardial Injury: Involvement of Suppression in Pyroptosis via Regulating HK1/NLRP3/Caspase-1 Signaling Pathway 
Discovery of CYP2E1 as a novel target in rheumatoid arthritis and validation by a new specific CYP2E1 inhibitor 
Heat-killed Bifidobacterium longum BBMN68 and inulin protect against high-fat diet-induced obesity by modulating gut microbiota 
Optical imaging detection of extracellular vesicles of miR-146 modified bone marrow mesenchymal stem cells promoting spinal cord injury repair 
MICT ameliorates hypertensive nephropathy by inhibiting TLR4/NF-κB pathway and down-regulating NLRC4 inflammasome 
PM10 exposure induces bronchial hyperresponsiveness by upreguating acetylcholine muscarinic 3 receptor 
Acupuncture ameliorates depression-like behavior of poststroke depression model rats through the regulation of gut microbiota and NLRP3 inflammasome in the colon 
Acupuncture ameliorates depression-like behavior of poststroke depression model rats through the regulation of gut microbiota and NLRP3 inflammasome in the colon 
Acupuncture ameliorates depression-like behavior of poststroke depression model rats through the regulation of gut microbiota and NLRP3 inflammasome in the colon 
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