EK204EGA

EasyGo!™ Mouse IL-4 One-Step ELISA Kit 检测试剂盒(酶联免疫吸附法)

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¥2,190.00¥3,650.00

  • 分子靶点:IL4, IL-4, BSF1, BCGF1
  • 种属:小鼠 (Mouse)
  • 样本类型:血清,血浆
  • 检测样本体积:5 μL
  • 灵敏度:5.75 pg/mL
  • 检测范围:31.25 pg/mL - 2000 pg/mL
  • 回收率:76% - 123%

在售SKU:70-EK204EG-48, 70-EK204EG-96, EK204EG


描述

商品名

EasyGo!™ Mouse IL-4 One-Step ELISA Kit 检测试剂盒(酶联免疫吸附法)

检测方法

双抗夹心法

精密度

板内变异系数:3.5% - 6.6%;板间变异系数:6.8% - 8.3%

样本类型

血清,血浆

检测样本体积

5 μL

灵敏度

5.75 pg/mL

检测范围

31.25 pg/mL - 2000 pg/mL

回收率

76% - 123%

平均回收率

0.96

板式

96孔板,可拆

保存

试剂盒未拆开,2 - 8℃保存。已拆开,标准品-20℃保存,其它2 - 8℃保存。

运输条件

4℃蓝冰运输

组分
  • 含微球的预包被酶标板
  • 标准品
  • 标准品稀释液
  • 显色底物
  • 终止液
  • 20×洗液

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上,酶标板孔中预先加入生物素标记的检测抗体微球和辣根过氧化物酶微球。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后,加入显色底物(TMB),避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应,在 450 nm 波长(参考波长 5630 nm)测定吸光度值。

EasyGo!™ Mouse IL-4 One-Step ELISA Kit EasyGo!™ 白细胞介素4 (IL-4) 一步法酶联免疫吸附测定试剂盒 - 标准曲线
标准曲线

分子信息

概述小鼠 IL4, IL-4, BSF1, BCGF1 靶点信息

IL4 分子靶点信息概述

  • 分子名:IL4, interleukin 4
  • 基因家族:Interleukins
  • 别名:BSF1; IL-4; BCGF1; BCGF-1; MGC79402
  • 全称:B_cell stimulatory factor 1; lymphocyte stimulatory factor 1; B cell growth factor 1

IL4 分子靶点综述

IL-4是II型辅助T细胞(Th2细胞)分泌的细胞因子。IL-4是一种多效性细胞因子,在调节T、B淋巴细胞和其他类型细胞的增殖、分化、凋亡,促进以Th2细胞为特征的免疫应答过程中发挥重要作用。IL-4多重生物效应依赖于细胞类型和分化状态,研究表明其在介导过敏性疾病、自身免疫性疾病、感染性疾病、肿瘤等疾病的免疫反应中有多重作用,对肿瘤、自身免疫性疾病和感染性疾病等有治疗作用,并且IL-4对疫苗免疫应答具有调节作用。

小鼠 Mouse Il4 分子靶点信息

小鼠 Mouse Il4 靶点分子功能(预测)

Enables cytokine activity. Involved in several processes, including innate immune response in mucosa; negative regulation of white fat cell proliferation; and regulation of gene expression. Acts upstream of or within several processes, including T-helper cell differentiation; positive regulation of macromolecule metabolic process; and regulation of leukocyte activation. Located in external side of plasma membrane and extracellular space. Is expressed in several structures, including brain; colon; hemolymphoid system; liver; and placenta. Used to study Sjogren's syndrome; atopic dermatitis; and type 1 diabetes mellitus. Human ortholog(s) of this gene implicated in several diseases, including asthma (multiple); autoimmune disease (multiple); hepatitis B; hepatitis C; and pancreatic cancer (multiple). Orthologous to human IL4 (interleukin 4).

操作步骤


ELISA操作常见问题

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引用文献


  1. Bionic sulfated glycosaminoglycan-based hydrogel inspired by snail mucus promotes diabetic chronic wound healing via regulating macrophage polarization 

  2. c-CBL/LCK/c-JUN/ETS1/CD28 axis restrains childhood asthma by suppressing Th2 differentiation 

  3. Enhancing protective immunity against bacterial infection via coating nano-Rehmannia glutinosa polysaccharide with outer membrane vesicles 

  4. Silencing TRIM8 alleviates allergic asthma and suppressing Th2 differentiation through inhibiting NF-κB/NLRP3 signaling pathway 

  5. Lentinan attenuates allergic airway inflammation and epithelial barrier dysfunction in asthma via inhibition of the PI3K/AKT/NF-κB pathway 

  6. Revealing the regulation of allergic asthma airway epithelial cell inflammation by STEAP4 targeting MIF through machine learning algorithms and single-cell sequencing analysis 

  7. A citrullinated antigenic vaccine in treatment of autoimmune arthritis 

  8. Necroptosis plays a role in TL1A-induced airway inflammation and barrier damage in asthma 

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