EK210EGA

EasyGo!™ Mouse IL-10 One-Step ELISA Kit 检测试剂盒（酶联免疫吸附法）

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¥2,190.00 – ¥3,650.00

  • 分子靶点：IL10, CSIF, TGIF
  • 种属：小鼠 (Mouse)
  • 样本类型：血清，血浆
  • 检测样本体积：5 μL
  • 灵敏度：44.07 pg/mL
  • 检测范围：125 pg/mL - 8000 pg/mL
  • 回收率：78% - 107%

在售SKU:70-EK210EGA-48, 70-EK210EGA-96, EK210EGA

描述

商品名

EasyGo!™ Mouse IL-10 One-Step ELISA Kit 检测试剂盒（酶联免疫吸附法）
检测方法

双抗夹心法
精密度

板内变异系数：3.4% - 4.2%；板间变异系数：3.9% - 4.4%
样本类型

血清，血浆
检测样本体积

5 μL
灵敏度

44.07 pg/mL
检测范围

125 pg/mL - 8000 pg/mL
回收率

78% - 107%
平均回收率

0.99
板式

96孔板，可拆
保存

试剂盒未拆开，2 - 8℃保存。已拆开，标准品-20℃保存，其它2 - 8℃保存。
运输条件

4℃蓝冰运输
组分
  • 含微球的预包被酶标板
  • 标准品
  • 标准品稀释液
  • 显色底物
  • 终止液
  • 20×洗液

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上，酶标板孔中预先加入生物素标记的检测抗体微球和辣根过氧化物酶微球。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后，加入显色底物（TMB），避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应，在 450 nm 波长 (参考波长 5630 nm) 测定吸光度值。

EasyGo!™ Mouse IL-10 One-Step ELISA Kit EasyGo!™ 白细胞介素10 (IL-10) 一步法酶联免疫吸附测定试剂盒 - 标准曲线
标准曲线

分子信息

概述小鼠 IL10, CSIF, TGIF 靶点信息

IL10 分子靶点信息概述

  • 分子名：IL10, interleukin 10
  • 基因家族：Interleukins
  • 别名：CSIF; TGIF; IL10A; IL-10
  • 全称：cytokine synthesis inhibitory factor; T-cell growth inhibitory factor

IL10 分子靶点综述

白细胞介素10(IL-10)是由2个长178个氨基酸的亚基构成的同源二聚体。在人类中，它由位于1号染色体的IL-10基因编码，由5个外显子组成。IL-10主要由单核细胞分泌，淋巴细胞、II型辅助性T细胞(Th2)、肥大细胞、CD4+CD25+Foxp3+调节性T细胞和部分活化的T细胞和B细胞分泌较少。它对免疫调控和炎症具有多种效应。它下调巨噬细胞的Th1细胞因子、II类MHC抗原和共刺激分子。它还能增强B细胞存活、增殖和抗体分泌。IL-10能阻断NF-κB活性，并参与JAK-STAT信号通路调控。IL-10的免疫抑制特性提示其可能应用于临床，抑制器官移植后的排斥。IL-10还具有强烈的消炎特性。

小鼠 Mouse Il10 分子靶点信息

小鼠 Mouse Il10 靶点分子功能（预测）

Enables cytokine activity. Involved in several processes, including negative regulation of cell death; negative regulation of heterotypic cell-cell adhesion; and negative regulation of vascular associated smooth muscle cell proliferation. Acts upstream of or within several processes, including cellular response to hepatocyte growth factor stimulus; negative regulation of cytokine production; and response to bacterium. Located in extracellular space. Is expressed in colon; liver; thymus; and thymus primordium. Used to study inflammatory bowel disease. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Orthologous to human IL10 (interleukin 10).

操作步骤


ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


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  2. Glypican-3-targeted macrophages delivering drug-loaded exosomes offer efficient cytotherapy in mouse models of solid tumours 

  3. Phased Ions-Release Bilayer-Guided Bone Regeneration Membrane with Nanostructure-Mediated Antibacterial Adhesion 

  4. A nanotheranostics with hypoxia-switchable fluorescence and photothermal effect for hypoxia imaging-guided immunosuppressive tumor microenvironment modulation 

  5. Localized light-triggered release macrophage cytopharmaceuticals containing O-nitrobenzyl group for enhanced solid tumor cell-chemotherapy 

  6. Astragaloside IV microfibers assembling into injectable 3D-scaffolds with intrinsic immunoactivity for enhanced tumor vaccine efficacy 

  7. Loss of Annexin A1 in macrophages restrains efferocytosis and remodels immune microenvironment in pancreatic cancer by activating the cGAS/STING pathway 

  8. Metal-ion-chelating phenylalanine nanostructures reverse immune dysfunction and sensitize breast tumour to immune checkpoint blockade 

  9. Spatio-temporally controlled all-in-one hydrogel microspheres for ulcerative colitis treatment based on cocktail strategy 

  10. The aberrant tonsillar microbiota modulates autoimmune responses in rheumatoid arthritis 

  11. Fucoidan improving spinal cord injury recovery: Modulating microenvironment and promoting remyelination 

  12. A citrullinated antigenic vaccine in treatment of autoimmune arthritis 

  13. Overexpression of miR-20a-5p in mesenchymal stem cell derived-exosomes from systemic lupus erythematosus patients restored therapeutic effect and Treg immune regulation 

  14. A polymeric nanoplatform enhances the cGAS-STING pathway in macrophages to potentiate phagocytosis for cancer immunotherapy 

  15. Mmu_circ_0005373 and hsa_circ_0136255 participate in the pulmonary fibrosis of systemic sclerosis 

  16. Surface nanocoating of bacteria as a versatile platform to develop living therapeutics 

  17. Peimine promotes microglial polarization to the M2 phenotype to attenuate drug-resistant epilepsy through suppressing the TLR4/NF-κB/HIF-1α signaling pathway in a rat model and in BV-2 microglia 

  18. Hydrolyzed Chicken Meat Extract and Its Bioactive Cyclopeptides Protect Neural Function by Attenuating Inflammation and Apoptosis via PI3K/AKT and AMPK Pathways 

  19. Neuroimmune modulating and energy supporting nanozyme-mimic scaffold synergistically promotes axon regeneration after spinal cord injury 

  20. miR-455/GREM1 axis promotes colorectal cancer progression and liver metastasis by affecting PI3K/AKT pathway and inducing M2 macrophage polarization 

  21. Boron Dipyrromethene-Based Nanotheranostic System for Sonophotoassisted Therapy and Simultaneous Monitoring of Tumor Immune Microenvironment Reprogramming 

  22. A polymeric nanoplatform enhances the cGAS-STING pathway in macrophages to potentiate phagocytosis for cancer immunotherapy 

  23. A polymeric nanoplatform enhances the cGAS-STING pathway in macrophages to potentiate phagocytosis for cancer immunotherapy 

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