EK201BEGA

EasyGo!™ Mouse IL-1β One-Step ELISA Kit 检测试剂盒（酶联免疫吸附法）

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¥2,190.00 – ¥3,650.00

  • 分子靶点：IL1B, IL1-β, IL1F2
  • 种属：小鼠 (Mouse)
  • 样本类型：血清，血浆
  • 检测样本体积：100 μL（稀释液）
  • 灵敏度：40.45 pg/mL
  • 检测范围：46.87 pg/mL - 3000 pg/mL
  • 回收率：97% - 114%

在售SKU:70-EK201BEGA-48, 70-EK201BEGA-96, EK201BEGA

描述

商品名

EasyGo!™ Mouse IL-1β One-Step ELISA Kit 检测试剂盒（酶联免疫吸附法）
检测方法

双抗夹心法
精密度

板内变异系数：6.4% - 7.9%；板间变异系数：3.5% - 4.3%
样本类型

血清，血浆
检测样本体积

100 μL（稀释液）
灵敏度

40.45 pg/mL
检测范围

46.87 pg/mL - 3000 pg/mL
回收率

97% - 114%
平均回收率

1.05
板式

96孔板，可拆
保存

试剂盒未拆开，2 - 8℃保存。已拆开，标准品-20℃保存，其它2 - 8℃保存。
运输条件

4℃蓝冰运输
组分
  • 含微球的预包被酶标板
  • 标准品
  • 标准品稀释液
  • 显色底物
  • 终止液
  • 20×洗液

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上，酶标板孔中预先加入生物素标记的检测抗体微球和辣根过氧化物酶微球。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后，加入显色底物（TMB），避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应，在 450 nm 波长 (参考波长 5630 nm) 测定吸光度值。

EasyGo!™Mouse IL-1β One-Step ELISA Kit EasyGo!™ 小鼠白细胞介素1β (IL-1β) 一步法酶联免疫吸附测定试剂盒 - 标准曲线
标准曲线

分子信息

概述小鼠 IL1B, IL1-β, IL1F2 靶点信息

IL1B 分子靶点信息概述

  • 分子名：IL1B, interleukin 1 beta
  • 基因家族：Interleukins
  • 别名：IL1F2; IL-1B; IL1-BETA

IL1B 分子靶点综述

白细胞介素1(IL-1)是17kDa的胞外多肽，分为IL-1α和IL-1β两种蛋白。IL-1β是活化的巨噬细胞分泌的蛋白前体，通过caspase1(CASP1/ICE)水解产生的活化形式。该细胞因子是炎症反应的重要介导者，参与许多细胞活性，包括细胞增殖、分化和凋亡。IL-1是一类重要的蛋白家族，它们由生物活化的单核细胞所衍生，参与炎症反应和免疫应答。IL-1β表达升高会产生各种不同的自身炎症综合征，最显著的是单基因条件称为CAPS，是由于触发IL-1β加工的炎性体受体NLRP3发生突变而引起的。本试剂盒用来检测人IL-1β的成熟形式和未裂解的前体形式。

小鼠 Mouse Il1b 分子靶点信息

小鼠 Mouse Il1b 靶点分子功能（预测）

Enables cytokine activity. Involved in several processes, including negative regulation of cell communication; regulation of defense response; and regulation of lipid metabolic process. Acts upstream of or within several processes, including ectopic germ cell programmed cell death; positive regulation of intracellular signal transduction; and positive regulation of macromolecule metabolic process. Located in extracellular space and secretory granule. Is expressed in several structures, including dorsal aorta; early conceptus; hemolymphoid system; integument; and reproductive system. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); blood platelet disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Orthologous to human IL1B (interleukin 1 beta).

操作步骤


ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. Bionic sulfated glycosaminoglycan-based hydrogel inspired by snail mucus promotes diabetic chronic wound healing via regulating macrophage polarization 

  2. Quercetin-primed BMSC-derived extracellular vesicles ameliorate chronic liver damage through miR-136-5p and GNAS/STAT3 signaling pathways 

  3. Aberrant copper metabolism and hepatic inflammation cause neurological manifestations in a mouse model of Wilson’s disease 

  4. Targeted Delivery of STING Agonist via Albumin Nanoreactor Boosts Immunotherapeutic Efficacy against Aggressive Cancers 

  5. EGB761 ameliorates mild cognitive impairment by inhibiting the pyroptosis and apoptosis in both in vivo and in vitro experiments 

  6. Serum amyloid A contributes to radiation-induced lung injury by activating macrophages through FPR2/Rac1/NF-κB pathway 

  7. ROS exhaustion reverses the effects of hyperbaric oxygen on hemorrhagic transformation through reactivating microglia in post-stroke hyperglycemic mice 

  8. Capsaicin mitigates ventilator-induced lung injury by suppressing ferroptosis and maintaining mitochondrial redox homeostasis through SIRT3-dependent mechanisms 

  9. QSOX1 exerts anti-inflammatory effects in sepsis-induced acute lung injury: regulation involving EGFR phosphorylation mediated M1 polarization of macrophages 

  10. Astragaloside IV microfibers assembling into injectable 3D-scaffolds with intrinsic immunoactivity for enhanced tumor vaccine efficacy 

  11. 2D Differential Metallic Immunopotentiators Drive High Diversity and Capability of Antigen-specific Immunity Against Tumor 

  12. Loss of Annexin A1 in macrophages restrains efferocytosis and remodels immune microenvironment in pancreatic cancer by activating the cGAS/STING pathway 

  13. Parkin deficiency exacerbates particulate matter-induced injury by enhancing airway epithelial necroptosis 

  14. Discovery of novel 5-phenyl-1H-pyrrole-2-carboxylic acids as Keap1-Nrf2 inhibitors for acute lung injury treatment 

  15. Proinflammatory Effect of Membrane Vesicles Derived from Enterococcus faecalis at Neutral and Alkaline pH 

  16. Exosomes from Hypoxic Pretreatment ADSCs Ameliorate Cardiac Damage Post-MI via Activated circ-Stt3b/miR-15a-5p/GPX4 Signaling and Decreased Ferroptosis 

  17. Construction of drug-free sodium bicarbonate nanoparticles with high water-tolerance for gas therapy to selectively induce non-apoptotic death of cancer cells 

  18. Metal-ion-chelating phenylalanine nanostructures reverse immune dysfunction and sensitize breast tumour to immune checkpoint blockade 

  19. Imaging Identifies Superior Inflammation Targeting of M1 Macrophages for Cryo-Shocked Cell Pulmonary Drug Delivery to Treat Acute Lung Injury 

  20. Tanshinone IIA Inhibits the Endoplasmic Reticulum Stress-Induced Unfolded Protein Response by Activating the PPARα/FGF21 Axis to Ameliorate Nonalcoholic Steatohepatitis 

  21. A non-Bactericidal Cathelicidin with Antioxidant Properties Ameliorates UVB-Induced Mouse Skin Photoaging via Intracellular ROS Scavenging and Keap1/Nrf2 Pathway Activation 

  22. Discovery of a Covalent Inhibitor of Pro-Caspase-1 Zymogen Blocking NLRP3 Inflammasome Activation and Pyroptosis 

  23. Novel analgesic peptide derived from Cinobufacini injection suppressing inflammation and pain via ERK1/2/COX-2 pathway 

  24. Development of a Self-Luminescent Living Bioreactor for Enhancing Photodynamic Therapy in Breast Cancer 

  25. Oleanane triterpenoids with C-14 carboxyl group from Astilbe grandis inhibited LPS-induced macrophages activation by suppressing the NF-κB signaling pathway 

  26. M2 macrophage infusion ameliorates diabetic glomerulopathy via the JAK2/STAT3 pathway in db/db mice 

  27. CD8?+?CD103?+?iTregs protect against ischemia-reperfusion-induced acute kidney Injury by inhibiting pyroptosis 

  28. Enzyme/ROS dual-sensitive nanoplatform with on-demand Celastrol release capacity for enhanced ulcerative colitis therapy by ROS scavenging, microbiota rebalancing, inflammation alleviating 

  29. Hydrolyzed Chicken Meat Extract and Its Bioactive Cyclopeptides Protect Neural Function by Attenuating Inflammation and Apoptosis via PI3K/AKT and AMPK Pathways 

  30. The Structural Characterization of a Polysaccharide from the Dried Root of Salvia miltiorrhiza and Its Use as a Vaccine Adjuvant to Induce Humoral and Cellular Immune Responses 

  31. Mid1 aggravates hepatic ischemia–reperfusion injury by inducing immune cell infiltration 

  32. DLK1 regulates periodontal inflammation by inhibiting NF-κB p65 and JNK signaling pathways 

  33. FGL2 Improves Experimental Colitis Related to Gut Microbiota Structure and Bile Acid Metabolism by Regulating Macrophage Autophagy and Apoptosis 

  34. miR-455/GREM1 axis promotes colorectal cancer progression and liver metastasis by affecting PI3K/AKT pathway and inducing M2 macrophage polarization 

  35. PCLAF induces bone marrow adipocyte senescence and contributes to skeletal aging 

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